A field experiment to test the optimization of chemical fertilizer use through Integrated Nutrient Management (INM) approach and its effect on growth and yield of Mustard (Brassica juncea L.) was carried out at Rampur agricultural field of Doon (P.G) College of Agriculture Science and Technology, Selaqui, Dehradun, Uttarakhand during the rabi season 2021-2022. The experiment comprised of nine treatments of organic and inorganic nutrient combination, viz T1- Control (No Fertilizer), T2- Recommended Dose of Fertilizer (RDF) (80:60:40 kg/ha), T3- 100 % vermicompost (6.0 t/ha), T4- 75% Farm Yard Manure (FYM) (15.0 t/ha) + 25% RDF (20:15:10 kg/ha), T5- 50% RDF (40:30:20 kg/ha) + FYM (5.0 t/ha) + green manure (2.5 t/ha) + gypsum (10.0 kg/ha), T6-50% RDF (40:30:20 kg/ha) + vermicompost (3.0 t/ha)+ gypsum (5.0 kg/ha), T7- 75% RDF (60:45:30 kg/ha) + vermicompost (2.5 t/ha), T8- 75% RDF (60:45:30 kg/ha) + vermicompost (1.5 t/ha) + green manure (1.5 t/ha), T9- 75% RDF (60:45:30 kg/ha) + FYM (1.5 t/ha) + vermicompost (1.0 t/ha) + green manure (1 t/ha) + gypsum (10.0 kg/ha), laid out in randomized block design with three replication. The results of experiment revealed that there was increase in plant growth parameters in the treatment T9, followed by treatment T8 and T5, respectively. Moreover, the maximum seed yield (2240.39 kg/ha) was observed under T9 over control, where no fertilizer application was applied. The present investigation established the fact that integrated use both organic and inorganic fertilizer can be recommended to the farmers as one of the key strategies for enhancing crop response in terms of growth and yield of mustard.