In this work, an adaptive nonlinear error feedback controller based on fuzzy state observer is proposed to a novel rubber pneumatic vibration isolator (PVI) system. First, a novel rubber PVI system is designed and developed. Harmonic balance method is applied to establish the dynamic model of the PVI system, so as to analyze the frequency response characteristics of the system. Second, to improve the PVI performance in low-frequency vibration, a nonlinear state error feedback control system based on fuzzy state observer is designed for the active PVI system. Furthermore, the experiment system is established. Finally, through the experimental comparison under 5 Hz, 7 Hz, 20 Hz, and 40 Hz, an adaptive PVI strategy is proposed. The experimental results demonstrate that the adaptive PVI shows better performance in vibration isolation.