Sustainable Agriculture Resource Research Articles

Mechanism of betterment towards growth and induction of defense in rice (Oryza sativa L.) by biopriming with bacterial endophytes isolated from wild rice

Utilizing beneficial microorganisms associated with plants, particularly endophytes, is becoming more and more prevalent since it supports the physiological health and evolutionary adaption of the host. The range of enhanced endophytic bacteria found in wild rice makes it a promising resource for sustainable agriculture. Current study focused on benefits of bacterial endophytes isolated from tissues of wild rice plants' roots, stems, and leaves for managing the health and development of rice (Oryza sativa L.) plants. Bacterial endophytes were characterized using 16 S rRNA. Treatments with Priestia megaterium (NRRI EB 1) and Priestia aryabhattai (NRRI EB 2) outperformed the other isolates in rice growth enhancement activities significantly. The biocontrol efficacy of bacterial endophytes was tested against Xanthomonas oryzae pv. oryzae and Rhizoctonia solani and percentage of inhibition was higher in NRRI EB 1 by 79.32–80.83 % and in NRRI EB 2 by 79.69–80.45 %. Bio-priming the seeds with specific endophytic bacterial strains led to a decrease in average germination time, an increase in seedling vigor, and total chlorophyll content. Additionally, they generated greater amounts of soluble phosphate (40.91–83.70 μg/mL) and indole acetic acid (28.10–60.18 μg/mL), which are in the midst of encouraging more plant development. Higher expressions of defense enzymes in comparison to the control, including catalase (>220 % in root and shoot), peroxidase (>200 % in shoot and root), and superoxide dismutase (>150 % in shoot and root) illustrates the rice crop's resilience to withstand stress. The activity of the mentioned enzymes was further validated through the activation of corresponding defense genes such as DEFENSIN (>2-fold), PAL (>3-fold), PR-3 (>2-fold), POX (>2-fold) and LOX (>1-fold) in relation to the untreated plants. The possibility exists to extract advantageous endophytic bacteria from wild rice species, potentially rewilding the microbiome of cultivated rice cultivars and fostering their deployment.

Toxic and non-toxic cyanobacterial biomass as a resource for sustainable agriculture: A lettuce cultivation experiment

Cyanobacteria represent a promising resource for sustainable agriculture, as they have demonstrated the ability to restore soil fertility even after death and decay. However, several cyanobacteria can also release secondary metabolites, such as cyanotoxins, which may compromise the quality of agricultural products and pose a potential risk to human health. Depending on the concentration of exposure, few studies reported deleterious effects on plant species when irrigated with cylindrospermopsin (CYN) contaminated water, impairing plant growth and leading to food product contamination, while other studies show promoting effects on plant yield. To evaluate the potential of cyanobacterial biomass (cyanotoxin-containing or not) as a sustainable resource for soil amendment, biostimulants or fertilizers for lettuce cultivation, a study was carried out that consisted of the culture of lettuce plants under controlled conditions, in soil: (1) with no extra nutrient addition (control) and supplemented with 0.6 g of freeze-dried Raphidiopsis raciborskii biomass of (2) a non-CYN-producing strain, (3) a CYN-producing strain, and (4) the same CYN-producing strain pasteurized. Results showed no significant differences in photosystem II efficiency with the amendment addition. On the contrary, shoot fresh weight significantly increased in lettuce plants grown with the cyanobacterial biomass addition, especially in condition (3). In addition, there were significant differences in mineral concentrations in lettuce leaves after the cyanobacterial biomass addition, such as K, Na, Ca, P, Mg, Mn, Zn, Cu, Mo, and Co. CYN accumulation was detected under conditions (3) and (4), with concentrations observed in descending order from roots > soil > shoot. Nevertheless, the CYN concentration in edible tissues did not exceed the WHO-proposed tolerable daily intake of 0.03 μg/kg/day. These findings suggest that incorporating cyanobacterial biomass as a soil amendment, biostimulant or fertilizer for lettuce cultivation, even with trace amounts of CYN (1–40 μg/g), may enhance plant yield without leading to cyanotoxin accumulation in edible tissues above the WHO-recommended tolerable daily intake.

Modeling climate change impacts under future CCM3 scenario on sorghum (Sorghum bicolor) as an drought resilient crop in tropical arid Lombok Island, Indonesia

Abstract. Wibowo AA, Meylani V. 2024. Modeling climate change impacts under future CCM3 scenario on sorghum (Sorghum bicolor) as an drought resilient crop in tropical arid Lombok Island, Indonesia. Intl J Trop Drylands 8: 35-43. The arid ecosystems and drought conditions exacerbated by climate change and rising CO2 levels necessitate the identification of alternative drought-tolerant crops. Sorghum bicolor L. has emerged as a promising option due to its resilience to drought. However, there is dearth of information regarding its future potential distribution, particularly in arid regions like Lombok Island, Indonesia, where sorghum is being considered as a viable alternative to ensure food security. This study employs Maximum Entropy (MaxEnt) modeling, incorporating environmental and bioclimatic variables, along with the National Center for Atmospheric Research (NCAR) Community Climate Model (CCM3) scenario reflecting doubled CO2 levels, to model the future potential distribution of S. bicolor. The model projects a total suitable habitat area of 1,875 km2, constituting 39.56% of Lombok Island’s land area. Notably, very high-suitability areas of 175 km2, and high-suitability areas of 200 km2 encompass 3.69% and 4.22% of the island’s territory, respectively, predominantly concentrated in the southern region of the island and characterized by low precipitation and high temperatures, particularly at altitudes ranging from 0 to 1,000 meters. The model's performance, evaluated using the Area Under the Curve (AUC), yields a score of 0.725, indicating a good level of accuracy. Key factors influencing sorghum distribution include annual precipitation (68.69%), isothermality (9.56%), temperature seasonality (9.56%), precipitation seasonality (8.69%), and annual mean temperature (3.47%). The CCM3 model forecasts an expansion of sorghum distribution toward the north, occupying approximately 6.25% of Lombok's total area. These findings highlight sorghum’s adaptability and resilience to future climate changes, positioning it as a valuable resource for sustainable agriculture in arid environments.

Unveiling the Potential of Coffee Grounds, Banana Peels and Egg Shells as an Eco‐Friendly and Sustainable Fertilizer

AbstractCoffee grounds, along with other organic materials like eggshells and banana peels, offer a promising solution for improving food security. This research brings innovation to the forefront by reimagining kitchen waste as a valuable resource for sustainable agriculture, introducing a multicomponent fertilizer approach, exploring different application methods, and focusing on a staple crop, ultimately contributing to the broader discourse on food security and environmental conservation. This study evaluated the effect of coffee grounds, eggshells, and banana peels as fertilizers on the growth and germination of Phaseolus vulgaris (common bean) plants. Two types of fertilizers are prepared: a dry mixture and a wet mixture obtained by boiling the dry mixture in water for 15 min. The height of the plants is measured weekly over a period of four weeks. The results indicated that the wet fertilizer mixture is more effective in promoting plant growth than the dry mixture. Plants that received the wet fertilizer mixture exhibited a higher growth rate and reached a greater height than those that received the dry mixture. It can be concluded that the wet fertilizer mixture is more efficient in promoting the growth of Phaseolus vulgaris plants than the dry mixture.

Neem's promise: The way to a sustainable future and eco-friendly biopesticides

Modern agriculture must include pest management since pests can seriously harm crops and lower yields, resulting in considerable financial losses. Conventional chemical insecticides have been used as the main method of pest management, but their detrimental effects on the environment and human health have inspired the quest for new environmentally friendly options. In this situation, Azadirachta indica showed promise as a sustainable and eco-friendly biopesticide. This research shows many aspects for Azadirachta indica as an environment friendly biopesticide. Neem is well known for having antiviral, antiseptic, and antifungal characteristics, which make it a crucial component in remedies for many illnesses, skin disorders, and mouth care. It is also beneficial to the environment. When added to the soil, its seeds and leaves help increase the fertility of the soil and structure. Additionally, the tree's large root system helps stop soil erosion. Azadirachta indica, is a great asset in the field of pest control and agricultural sustainability due to its repellent and antifeedant qualities. These characteristics are a result of neem's bioactive substances, known as Azadirachtin which has been the subject of in-depth study and use because of its function as a natural repellent and antifeedant Azadirachtin is a potent natural insect growth regulator because it interferes with beetle feeding, molting, and reproduction. Furthermore, the eco-friendliness of azadirachtin and its safety for people and non-target animals highlight its essential function in sustainable pest management techniques, aiding in the advancement of environmentally friendly and healthier agricultural systems. The neem plant is a unique botanical gem that has enormous medicinal, farming, and cultural significance. Its adaptability and environmental friendliness make it an important resource for sustainable agriculture and human well-being. Three commercialized neem-based formulations were tested aphid, Myzus persicae, including Azatrol, Triple Action Neem Oil (70% neem oil), and Pure Neem Oil (100% neem oil) in greenhouse and laboratory settings. A bioassay using a leaf disc choice test indicated that of the established neem-based insecticides tested were effective against the aphid at the recommended concentrations. However, a doubling in the quantity of Azatrol and Triple Action Neem Oil resulted in a 50% decrease in the number of aphids landing on handled leaf tissue compared to untreated leaf tissue.

Draft genome sequences of representative Paenibacillus polymyxa, Bacillus cereus, Fictibacillus sp., and Brevibacillus agri strains isolated from Amazonian dark earth.

Here, we report 10 distinct bacterial genomes from Amazonian dark earths, including six identified as Paenibacillus polymyxa, while the remaining four were unique representatives of Paenibacillus vini, Bacillus cereus, Brevibacillus agri, and Fictibacillus sp., respectively. Each strain exhibited antagonistic activity against Fusarium oxysporum, underscoring their potential as sustainable agriculture resources.

A New Composite Material with Environmental Implications for Sustainable Agriculture.

Sewage sludge, also referred to as biosolids, consists of the by-products of wastewater treatment, which are a mixture of the water and organic and inorganic materials eliminated from wastewater sourced from domestic sewage industries during physical, biological, and/or chemical treatments. Biosolids are nutrient-rich organic materials resulting from the treatment of domestic wastewater in treatment plants. Sewage sludge can be considered a significant biological resource for sustainable agriculture. A new composite has been made consisting of soil, sewage sludge collected from the Galati Wastewater Treatment Plant, and slag from the Galati Steel Plant. This study aimed to investigate the structural, morphological, and chemical parameters of this composite through FTIR, SEM-EDX analysis, and XRF analysis. The samples had values of potential toxic elements that were within normal limits according to Directive 86/278/CEE, and, in terms of the iron-to-calcium ratio (I/C), all samples were of a low grade. This is the first time that slag has been added in a sewage sludge-soil combination, which can be an effective fertilizer replacement. Sewage sludge contains substantial amounts of organic matter, and slag reduces the contents of potentially toxic elements. In addition to these attributes, they may provide an opportunity for the beneficial re-use of sewage sludge and slag as resources in agriculture.

Miscanthus-Derived Biochar Enhanced Soil Fertility and Soybean Growth in Upland Soil

As biochar improves soil fertility and crop productivity, there is a growing interest in it as a resource for sustainable agriculture. Miscanthus sacchariflorus has promising applications in various industries because it has a large amount of biomass. However, research on the agricultural utilization of Miscanthus-derived biochar is insufficient. The aim of this study was to demonstrate the effects of Miscanthus biochar on the soil environment and soybean growth. First, Miscanthus biochar was amended at different levels (3 or 10 tons/ha) in upland soil, after which the soil properties, root development, and yield of soybeans were compared with the control (without biochar). In the soil amended with 10 tons/ha of biochar (BC10), organic matter (OM) and available phosphate increased 1.6 and 2.0 times, respectively, compared with that in the control soil (CON). In addition, the soil dehydrogenase activity increased by 70% in BC10, and 16S rRNA gene sequence analysis revealed that the structure of the microbial community changed after amendment with biochar. The bacterial phyla that differed between CON and BC10 were Acidobacteria and Chloroflexi, which are known to be involved in carbon cycling. Owing to these changes in soil properties, the root dry weight and number of nodules in soybeans increased by 23% and 27%, respectively, and the seed yield increased 1.5-fold in BC10. In conclusion, Miscanthus biochar increased the fertility of soybean-growing soil and consequently increased seed yield. This study is valuable for the practical application of biochar for sustainable agriculture.

Endophytic bacterial communities in wild rice (Oryza officinalis) and their plant growth-promoting effects on perennial rice.

Endophytic bacterial microbiomes of plants contribute to the physiological health of the host and its adaptive evolution and stress tolerance. Wild rice possesses enriched endophytic bacteria diversity, which is a potential resource for sustainable agriculture. Oryza officinalis is a unique perennial wild rice species in China with rich genetic resources. However, endophytic bacterial communities of this species and their plant growth-promoting (PGP) traits remain largely unknown. In this study, endophytic bacteria in the root, stem, and leaf tissues of O. officinalis were characterized using 16S rRNA gene Illumina sequencing. Culturable bacterial endophytes were also isolated from O. officinalis tissues and characterized for their PGP traits. The microbiome analysis showed a more complex structure and powerful function of the endophytic bacterial community in roots compared with those in other tissue compartments. Each compartment had its specific endophytic bacterial biomarkers, including Desulfomonile and Ruminiclostridium for roots; Lactobacillus, Acinetobacter, Cutibacterium and Dechloromonas for stems; and Stenotrophomonas, Chryseobacterium, Achromobacter and Methylobacterium for leaves. A total of 96 endophytic bacterial strains with PGP traits of phosphate solubilization, potassium release, nitrogen fixation, 1-aminocyclopropane-1-carboxylate (ACC) deaminase secretion, and siderophore or indole-3-acetic acid (IAA) production were isolated from O. officinalis. Among them, 11 strains identified as Enterobacter mori, E. ludwigii, E. cloacae, Bacillus amyloliquefaciens, B. siamensis, Pseudomonas rhodesiae and Kosakonia oryzae were selected for inoculation of perennial rice based on their IAA production traits. These strains showed promising PGP effects on perennial rice seedlings. They promoted plants to form a strong root system, stimulate biomass accumulation, and increase chlorophyll content and nitrogen uptake, which could fulfil the ecologically sustainable cultivation model of perennial rice. These results provide insights into the bacterial endosphere of O. officinalis and its application potential in perennial rice. There is the prospect of mining beneficial endophytic bacteria from wild rice species, which could rewild the microbiome of cultivated rice varieties and promote their growth.

Genome and Genetic Engineering of the House Cricket (Acheta domesticus): A Resource for Sustainable Agriculture.

Background: The house cricket, Acheta domesticus, is one of the most farmed insects worldwide and the foundation of an emerging industry using insects as a sustainable food source. Edible insects present a promising alternative for protein production amid a plethora of reports on climate change and biodiversity loss largely driven by agriculture. As with other crops, genetic resources are needed to improve crickets for food and other applications. Methods: We present the first high quality annotated genome assembly of A. domesticus from long read data and scaffolded to chromosome level, providing information needed for genetic manipulation. Results: Gene groups related to immunity were annotated and will be useful for improving value to insect farmers. Metagenome scaffolds in the A. domesticus assembly, including Invertebrate Iridescent Virus 6 (IIV6), were submitted as host-associated sequences. We demonstrate both CRISPR/Cas9-mediated knock-in and knock-out of A. domesticus and discuss implications for the food, pharmaceutical, and other industries. RNAi was demonstrated to disrupt the function of the vermilion eye-color gene producing a useful white-eye biomarker phenotype. Conclusions: We are utilizing these data to develop technologies for downstream commercial applications, including more nutritious and disease-resistant crickets, as well as lines producing valuable bioproducts, such as vaccines and antibiotics.

Distinctive Structure and Assembly of Phyllosphere Microbial Communities between Wild and Cultivated Rice.

Wild rice has been demonstrated to possess enriched genetic diversity and multiple valuable traits involved in disease/pest resistance and abiotic stress tolerance, which provides a potential resource for sustainable agriculture. However, unlike the plant compartments such as rhizosphere, the structure and assembly of phyllosphere microbial communities of wild rice remain largely unexplored. Through amplicon sequencing, this study compared the phyllosphere bacterial and fungal communities of wild rice and its neighboring cultivated rice. The core phyllosphere microbial taxa of both wild and cultivated rice are dominated with Pantoea, Methylobacterium, Nigrospora, and Papiliotrema, which are potentially beneficial to rice growth and health. Compared to the cultivated rice, Methylobacterium, Sphingomonas, Phaeosphaeria, and Khuskia were significantly enriched in the wild rice phyllosphere. The potentially nitrogen-fixing Methylobacterium is the dominated wild-enriched microbe; Sphingomonas is the hub taxon of wild rice networks. In addition, the microbiota of wild rice was more governed by deterministic assembly with a more complicated and stable community network than the cultivated rice. Our study provides a list of the beneficial microbes in the wild rice phyllosphere and reveals the microbial divergence between wild rice and cultivated rice in the original habitats, which highlights the potential selective role of wild rice in recruiting specific microbiomes for enhancing crop performance and promoting sustainable food production. IMPORTANCE Plant microbiota are being considered a lever to increase the sustainability of food production under a changing climate. In particular, the microbiomes associated with ancestors of modern cultivars have the potential to support their domesticated cultivars. However, few efforts have been devoted to studying the biodiversity and functions of microbial communities in the native habitats of ancestors of modern crop species. This study provides a list of the beneficial microbes in the wild rice phyllosphere and explores the microbial interaction patterns and the functional profiles of wild rice. This information could be useful for the future utilization of the plant microbiome to enhance crop performance and sustainability, especially in the framework of sustainable agroecosystems.

Thank you for smoking: Potential of biochar in sustainable agriculture

Biochar is an emerging material critical for sustainable agriculture and climate change mitigation. Here, I discuss the potential advantages of biochar and propose improvement of this material by integrating other biostimulants of combustion, providing a robust and cost-effective resource for sustainable agriculture.

ENSURING SUSTAINABLE DEVELOPMENT OF AGRICULTURE BASED ON THE USE OF SEWAGE SLUDGE

Topicality. The events of recent years (the Covid-19 pandemic, hostilities in Ukraine) have led to a significant increase in world food prices. Thus, the FAO price index in February 2022 reached a historic high of 140.7 percentage points. One of the significant factors in this situation is a significant increase in prices for fertilizers for agriculture, which leads to their shortage. This actualizes the search for a solution to the problem of using sewage sludge as biological resource for sustainable agriculture and ensuring the implementation of sustainable development goals, including goals 2, 6, 11, 12, 15.Aim and tasks. The purpose of the article is to analyze various aspects of the use of sewage sludge in agricultural production as one of the promising ways to achieve sustainable development goals.Research results. The content of organic matter in the sludge can improve the physical, chemical, and biological properties of the soil by ensuring better soil cultivation and water retention capacity, especially when dehydrated sewage sludge is used. Biosolids reduce runoff and increase surface water retention. Organic nitrogen in sludge is much less likely to cause groundwater pollution than chemical nitrogen fertilizers. The use of sewage sludge on agricultural land is the best way to recycle the nutrients it contains. Sewage sludge can therefore be considered an important biological resource for sustainable agriculture. Physico-chemical characteristics of sewage sludge of different origin were compared. The concentration of carbon, nitrogen, and phosphorus were similar. Nevertheless, there are some site-specific factors that make each sewage sludge unique and must be taken into account to predict the outcome of sewage sludge treatment. To achieve the goal, an analysis was made of the effect of sewage sludge on the physico-chemical and microbial properties of the soil; its impact on plants and their productivity; the problems of using soil sludge in agriculture, taking into account its contamination with various substances (heavy metals, microplastics and pharmaceuticals), have been studied.Conclusions. Sewage sludge is a promising fertilizer for sustainable agriculture, taking into account the need for an effective technology for its purification from hazardous substances.

An Actinobacterium Strain From Soil of Cerrado Promotes Phosphorus Solubilization and Plant Growth in Soybean Plants.

The huge biological diversity of the Brazilian Cerrado is an important source of economically interesting microbial agents. The phylum Actinobacteria plays an important role in nutrient cycling, potentially improving their availability to plants. In this study, we isolated an actinobacteria (strain 3AS4) from wheat rhizospheres of crops cultivated in the Cerrado biome. Strain 3AS4 was identified as belonging to the genus Streptomyces and had phosphorus mobilization ability, mineralizing approximately 410 μg ml–1 from phytate, 300 μg ml–1 from calcium phosphate, and 200 μg ml–1 from rock phosphate. The analysis of the actinobacteria crude extract by spectrometric techniques revealed the presence of gluconic and 2-ketogluconic acid, and a greenhouse experiment was carried out to evaluate its plant growth promotion activity in soybean. Soil in its natural condition (with no phosphorus addition), 40 kg ha–1 rock phosphate from Bayovar (RP) added to soil, and triple super phosphate (SPT) added to soil were used. Significant differences in plant height were observed at 6 weeks when the plants were inoculated with the 3AS4 strain. The growth of inoculated plants in natural condition was promoted in 17% compared with the RP and SPT non-inoculated conditions, suggesting that inoculation can enable plants to grow with lower chemical P fertilizers. In the plants that were inoculated with the 3AS4 strain in the RP condition, the plant height increased by approximately 80% and the shoot:root ratio was approximately 30% higher compared to control conditions (non-inoculated plants in natural conditions). 3AS4 has P-solubilizing potential and can be exploited as an inoculant for soybean cultivation. These results suggest that this actinobacterium is a valuable resource for sustainable agriculture and will allow the reduction of phosphate fertilization in the future.

Mechanism of Plant Growth Promotion and Disease Suppression by Chitosan Biopolymer

The chitosan (CHT) biopolymer is a de-acetylated chitin derivative that exists in the outer shell of shrimp, shellfish, lobster or crabs, as well as fungal cell walls. Because of its biodegradability, environmental non-toxicity, and biocompatibility, it is an ideal resource for sustainable agriculture. The CHT emerged as a promising agent used as a plant growth promoter and also as an antimicrobial agent. It induces plant growth by influencing plant physiological processes like nutrient uptake, cell division, cell elongation, enzymatic activation and synthesis of protein that can eventually lead to increased yield. It also acts as a catalyst to inhibit the growth of plant pathogens, and alter plant defense responses by triggering multiple useful metabolic pathways. This review emphasizes the role and mechanisms of CHT as a plant growth promoter and disease suppressor, and its future implications in agriculture.

Characterization of Soil Microorganism from Humus and Indigenous Microorganism Amendments

This study was conducted to understand the dynamics of microbial communities of soil microorganisms, and their distribution and abundance in the indigenous microorganisms (IMOs) manipulated from humus collected from the forest near the crop field. The soil microorganisms originated from humus and artificially cultured microbial-based soil amendments were characterized by molecular and biochemical analyses. The bacterial population (2 × 106∼13 × 106 CFU/g sample) was approximately 100-fold abundant than the fungal population (2 × 104∼8 × 104 CFU/g sample). The 16S rDNA and ITS sequence analyses showed that the bacterial and fungal communities in humus and IMOs were mainly composed of Bacillus and Pseudomonas, and Trichoderma and Aspergillus species, respectively. Some of the bacterial isolates from the humus and IMOs showed strong inhibitory activity against soil-borne pathogenic fungi Fusarium oxysporum and Sclerotinia sclerotiorum. These bacteria also showed the siderophore production activity as well as phosphate solubilizing activity, which are requisite traits for biological control of plant pathogenic fungi. These results suggest that humus and IMOs could be a useful resource for sustainable agriculture.

Preface

The International Conference on Sustainable Cereals and Crops Production Systems in the Tropics (ICFST) was held on 23-25 September 2019 in Makassar, Indonesia. The Conference was organized by Indonesian Agency for Agricultural Research and Development (IAARD)-Ministry of Agriculture of Indonesia, collaborated with International Maize and Wheat Improvement Center (CIMMYT) and Ministry of Research, Technology and Higher Education of Indonesia.The theme of the conference is “Sustainable Agricultural Resources Management to Support Food Security and Industry 4.0” with the sub themes of Breeding and Biotechnology, Crop Production Systems, Pest and Disease Management, Post Harvest, Socio-Economy and Community Development. The conference was conducted in three days, two days of scientific session and one day of field visit to the Indonesian Cereals Research Institute. The conference facilitate the research community focusing in food crops and provide platform for scientists to meet and interact with each other to share their knowledge and their research results along with the obstacles and challenges they faced in their development, achievement as well as experiences through the presentation of papers and discussion. This international conference is also an event to establish cooperation in the development of food crops research in the future as well as enhancing the knowledge of environmental protection with the current agricultural technologies.We would like to convey our deepest gratitude to the Minister of Agriculture of Indonesia, Keynote Speakers: Dr B.M. Prasanna (Director of CIMMYT’s Global Maize Program & the CGIAR Research Program), Prof. Dr Machito Mihara (Tokyo Agricultural University), Thomas E. Anderson, PhD (US Department of Agriculture), Prof Roger Shivas (University of Southern Queensland), Prof Bunyamin Tar’an (University of Saskatchewan Canada), sponsors, organizing committee and also to all participants.We also would like to express our deepest gratitude to the Indonesian Agency for Agricultural Research and Development (IAARD) conducted such conference. We are looking forward to the 2nd ICFST that will be held on September 2020 in Bali Island . We expect that these future ICFST conference will be as stimulating as this most recent one was, as indicated by the contributions presented in this proceedings volume.List of Committees Steering Committee (SC) and conference photographs are available in this PDF.

Unsustainability Syndrome—From Meteorological to Agricultural Drought in Arid and Semi-Arid Regions

Water is the most important resource for sustainable agriculture in arid and semi-arid regions, where agriculture is the mainstay for rural societies. By relating the water usage to renewable water resources, we define three stages from sustainable to unsustainable water resources: (1) sustainable, where water use is matched by renewable water capacity, ensuring sustainable water resources; (2) transitional, where water use occasionally exceeds renewable water capacity; and (3) unsustainable, with lack of water resources for agriculture, society, and the environment. Using available drought indicators (standardized precipitation index (SPI) and streamflow drought index (SDI)) and two new indices for agricultural drought (overall agricultural drought index (OADI) and agricultural drought index (ADI)), we evaluated these stages using the example of Fars province in southern Iran in the period 1977–2016. A hyper-arid climate prevailed for an average of 32% of the province’s spatio-temporal coverage during the study period. The area increased significantly from 30.6% in the first decade (1977–1986) to 44.4% in the last (2006–2015). The spatiotemporal distribution of meteorological drought showed no significant negative trends in annual precipitation during 1977–2016, but the occurrence of hydrological droughts increased significantly in the period 1997–2016. The expansion of irrigated area, with more than 60% of rainfed agriculture replaced by irrigated agriculture (especially between 1997 and 2006), exerted substantial pressure on surface water and groundwater resources. Together, climate change, reduced river flow, and significant declines in groundwater level in major aquifers led to unsustainable use of water resources, a considerable reduction in irrigated area, and unsustainability in agricultural production in the period 2006–2015. Analysis of causes and effects of meteorological, hydrological, and agricultural drought in the area identified three clear stages: before 1997 being sustainable, 1997–2006 being transitional, and after 2006 being unsustainable.

Isolation and identification of indole acetic acid producing bacteria from the coasts of Ben Tre and Tra Vinh Provinces

Beneficial plant-growth-promoting bacteria (PGPB) have been reasonably applied to rescue crucial issue for agriculture by salinity soil. Observed most of PGPB was found in endophyte, rhizosphere and soil. Indole acetic acid (IAA)-producing bacteria could naturally stimulate and facilitate plant growth. The knowledge of IAA production and content of bacteria resident in the marine environment has been typically insufficient and limited to date. In recent years, unwarrantable intrusions of sea water have been enlarged in the Mekong River Delta of Vietnam, threatening productive rice fields, local fruits, and cash crops. Therefore, finding PGPB in the coastal regions in the Mekong River Delta as a creative resource for sustainable agriculture is necessary and is a prompt challenge. In this study, IAA-producing bacteria from coastal regions of Ben Tre and Tra Vinh Provinces were isolated and adequately identified. Out of 202 bacterial isolates, 10 isolates showed the possible ability to produce IAA from L-tryptophan. These 10 isolates were objectively evaluated the capacity to produce IAA under 5% (w/v) NaCl in King B and marine broths. The results revealed that IAA production decreased in 5% NaCl, even though bacterial growth increased. These 10 IAA-producing bacteria were classified at the species level, Marinobacter hydrocarbonoclasticus, M. pelagius, M. daepoensis, and Mameliella phaeodactyli by 16S rRNA gene analysis. The most IAA producer in King’s B broth, the isolate C7, was investigated in more detail. The isolate C7 produced the maximum IAA amount (192.2 ± 1.14 µg/ml) under the presence of 20 g/l yeast extract, 2 g/l of L-tryptophan and 1% NaCl. The isolate C7 was able to grow at 1–17% (w/v) NaCl (optimum, 4%), but not in the absence of NaCl, indicating it is a moderate halophilic bacteria. This study highlighted the considerable ability to produce IAA of marine bacteria, which could be thoughtfully considered to use naturally as biofertilizers to promote plant growth in saline intrusion lands.

Isolation and identification of indole acetic acid producing bacteria from the coasts of Ben Tre and Tra Vinh Provinces

Beneficial plant-growth-promoting bacteria (PGPB) have been reasonably applied to rescue crucial issue for agriculture by salinity soil. Observed most of PGPB was found in endophyte, rhizosphere and soil. Indole acetic acid (IAA)-producing bacteria could naturally stimulate and facilitate plant growth. The knowledge of IAA production and content of bacteria resident in the marine environment has been typically insufficient and limited to date. In recent years, unwarrantable intrusions of sea water have been enlarged in the Mekong River Delta of Vietnam, threatening productive rice fields, local fruits, and cash crops. Therefore, finding PGPB in the coastal regions in the Mekong River Delta as a creative resource for sustainable agriculture is necessary and is a prompt challenge. In this study, IAA-producing bacteria from coastal regions of Ben Tre and Tra Vinh Provinces were isolated and adequately identified. Out of 202 bacterial isolates, 10 isolates showed the possible ability to produce IAA from L-tryptophan. These 10 isolates were objectively evaluated the capacity to produce IAA under 5% (w/v) NaCl in King B and marine broths. The results revealed that IAA production decreased in 5% NaCl, even though bacterial growth increased. These 10 IAA-producing bacteria were classified at the species level, Marinobacter hydrocarbonoclasticus, M. pelagius, M. daepoensis, and Mameliella phaeodactyli by 16S rRNA gene analysis. The most IAA producer in King’s B broth, the isolate C7, was investigated in more detail. The isolate C7 produced the maximum IAA amount (192.2 ± 1.14 µg/ml) under the presence of 20 g/l yeast extract, 2 g/l of L-tryptophan and 1% NaCl. The isolate C7 was able to grow at 1–17% (w/v) NaCl (optimum, 4%), but not in the absence of NaCl, indicating it is a moderate halophilic bacteria. This study highlighted the considerable ability to produce IAA of marine bacteria, which could be thoughtfully considered to use naturally as biofertilizers to promote plant growth in saline intrusion lands.