Resin Essential Oil Research Articles

Antifungal and Antibacterial Potential of Essential Oil of Boswellia sacra Resin in the Biological Control

<p><span lang="EN-US" style="font-size:12.0pt"><span style="background:white"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif"">This study focuses on extraction of Boswillia sacra resin essential oil that was performed using the hydrodistillation method, resulting in a yield of 2.45%. Phytochemical screening of the essential oil provided information about its major compounds, which include terpenes and anthocyanins. To assess the antioxidant and antimicrobial effects of the essential oil, multiple methods were employed. Positive results were obtained from tests such as DPPH and ABTS free radical scavenging, inhibition of bovine serum albumin protein denaturation, and antifungal and antibacterial assays. These tests confirmed the presence of antifungal, antibacterial and antioxidant activities and </span></span></span></span><span lang="EN-US" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif"">suggest numerous biotechnological applications of the essential oil.</span></span></span></p>

Chemical Composition, Repellent, and Phytotoxic Potentials of the Fractionated Resin Essential Oil from Araucaria heterophylla Growing in Tunisia.

The resin essential oil (REO) of the Tunisian Araucaria heterophylla trunk bark was investigated for its chemical composition. Then, it was evaluated for its insecticidal and allelopathic activities. The REO was obtained by hydrodistillation for 9 h (yield of 4.2 % w/w). Moreover, fractional hydrodistillation was carried out at 3-hour intervals, resulting in 3 fractions (R1-R3), to facilitate chemical identification and localization of the aforementioned biological activities. GC/MS analysis of the obtained samples allowed the identification of 25 compounds, representing between 91.2 and 96.3 % of their total constituents, which consisted predominantly of sesquiterpene hydrocarbons, oxygenated sesquiterpenes and diterpene hydrocarbons. α-Copaene (10.8 %), γ-muurolene (5.8 %), α-copaen-11-ol (7.8 %), spathulenol (10.5 %), 15-copaenol (8.2 %), ylangenal (10.3 %), dehydrosaussurea lactone (7.7 %), and sandaracopimaradiene (11.4 %) were identified as major compounds. The second part aimed to assess the impact of the A. heterophylla EO and its three fractions for their insecticidal and repellent activity against Tribolium castaneum (Herbst), a stored grain pest, of which a strong repellent activity was noted. In addition, the studied samples showed high phytotoxic effects against Lactuca sativa. The third fraction (R3) performed a total inhibitory potential on seed germination and seedling growth of the target plant. Furthermore, alongside this discovery, an estimation was conducted through molecular docking analysis. Wherein the main compounds of the studied samples were docked into the active pocket of protoporphyrinogen IX oxidase (PDB: 1SEZ), a key enzyme in chlorophyll biosynthesis. Thus, it is recommended to use the REO of A. heterophylla as a natural herbicide.

Araucaria heterophylla oleogum resin essential oil is a novel aldose reductase and butyryl choline esterase enzymes inhibitor: in vitro and in silico evidence

The essential oil isolated by hydrodistillation of the oleogum resin of Araucaria heterophylla has been analyzed by GC–MS. Twenty-four components accounting to 99.89% of the total detected constituents of this essential oil were identified. The major ones were: caryophyllene oxide (14.8%), ( +)-sabinene (12.07%), D-limonene (11.22%), caryophyllene (10.36%), α-copaene (8.00%), β-pinene (6.44%), trans-verbenol (5.88%) and α-pinene oxide (5.18%). The in vitro inhibitory activities of this oil against aldose reductase, BuCHE, COX-2 and SARS-CoV-2 Mpro enzymes were evaluated. This revealed promising inhibitory activity of the essential oil against both aldose reductase and BuCHE enzymes. The molecular docking study of the major components of the Araucaria heterophylla essential oil was carried out to correlate their binding modes and affinities for aldose reductase and BuCHE enzymes with the in vitro results. In conclusion, the in vitro inhibitory activity of the essential oil attributed to the synergistic effect between its components and the in silico study suggested that compounds containing epoxide and hydroxyl groups may be responsible for this activity. This study is preliminary screening for the oil to be used as antidiabetic cataract and Alzheimer’s disease therapeutics and further investigations may be required.

Evaluating the Potential of Boswellia rivae to Provide Sustainable Livelihood Benefits in Eastern Ethiopia.

Frankincense is an oleo-gum-resin collected from wild Boswellia spp. trees, and widely used in perfumery, cosmetics, aromatherapy, incense, and other industries. Boswellia rivae, growing in Ethiopia, Somalia, and Kenya, is one source of frankincense, but is little-commercialized compared to species such as B. sacra, B. frereana, and B. papyrifera. In this study, we examine the resin essential oil chemistry and harvesting systems of B. rivae in order to evaluate its potential for increased trade and potential positive livelihood benefits. Boswellia rivae produces an essential oil rich in α-thujene (0.1-12.4%), α-pinene (5.5-56.4%), β-pinene (0.3-13.0%), δ-3-carene (0.1-31.5%), p-cymene (1.4-31.2%), limonene (1.8-37.3%), β-phellandrene (tr-5.6%), trans-pinocarveol (0.1-5.0%), trans-verbenol (0.1-11.2%), and trans-β-elemene (0-5.7%), similar to major commercial species, although it is difficult to detect mixing of B. rivae and Commiphora africana resins from chemistry alone. The B. rivae trees are not actively tapped, so resin collection has a neutral impact on the health of the trees, and resin production is unaffected by drought. Consequently, collecting resins acts as a key income supplementing livestock herding, as well as a safety net protecting pastoral communities from the severe negative effects of climate change-exacerbated drought on livestock. Therefore, Boswellia rivae is well positioned chemically, ecologically, and socially to support expanded trade.

Inter-Tree Variation in the Chemical Composition of Boswellia papyrifera Oleo-Gum-Resin

Frankincense is a fragrant resin produced by Boswellia species, and has been used for centuries as a perfume, medicine, and incense, and is an important cosmetic and therapeutic product today. A number of studies have been conducted on the resin essential oils, but many have used commercial sources outside of the country of origin, leading to potential taxonomic confusion or misidentification. Individual Boswellia papyrifera resin samples were each obtained directly from 11 individual trees in Sudan, hydrodistilled, the volatile phytochemicals determined by gas chromatographic methods, and the chemical compositions subjected to cluster analysis. All samples were very similar, with high levels of octyl acetate (49.5%-81.0%) and octanol (6.5%-13.7%), and varying levels of diterpenoids (6.6%-32.7%). The cluster analysis indicated 3 highly similar groups, defined by (1) relatively higher levels of octyl acetate (58.9%-81.0%), but with low levels of diterpenoids (6.6%-18.6%); (2) relatively lower levels of octyl acetate (49.5%-61.3%), but with a higher proportion of diterpenoids (19.0%-22.8%); and (3) with octyl acetate (51.6%) and diterpenoids (32.7%).

Chemical Composition and Antimicrobial Properties of Essential Oils Obtained from the Resin of Picea orientalis L.

This study aims to evaluate the chemical composition and antimicrobial (antibacterial and antifungal) properties of the essential oil subtracted from the oleoresins, which were obtained from the stem of the Picea orientalis naturally grown in Turkey. The essential oil of the resin was obtained through the hydrodistillation method in a Clevenger type device, and it was analyzed with GC-MS/FID. The structure of 74 compounds that were found in the essential oil was elucidated by comparing with literature data and the database in the appliance. Limonene (53.40%), β-pinene (20.74%) and α-pinene (14.51%) were determined to be the major components. The highest class of compounds in resin essential oils was identified as monoterpene hydrocarbons. The antimicrobial activity of the essential oil was investigated with the agar well diffusion method against 25 microorganisms, including 13 gram-negative, 7 gram-positive bacteria and 5 yeast-molds. As the results of the analysis, the highest antibacterial activity was recorded against S. typhimurium from gram-negative bacteria with inhibition zone ranging as (13.70±0.10-9.70±0.10 mm) and S. aureus from gram-positive bacteria with inhibition zone ranging as (9.30±0.10-6.80±0.10 mm). Additionally, the highest antifungal activity was obtained against C. albicans from yeast-molds with inhibition zone ranging as (10.10±0.10-6.10±0.10 mm).

Evaluation of volatile constituents, exudation of resin and occurrence of galls of Protium aracouchini (Aubl.) Marchand

Protium aracouchini (Aubl.) Marchand [sin Icica aracouchini Aubl.], which occurs in the Adolpho Ducke Forest Reserve, in the Brazilian Amazon, was evaluated for the presence of galls, for resin exudation and the composition of the essential oils from the aerial parts and the resin. The experiment to stimulate the exudation of resin from the trunk was conducted using a 2-chloroethylphosphonic acid solution. The resin produced after 40 days and the aerial parts had their essential oils extracted in a Clevenger apparatus and the volatile chemical constituents were analyzed using GC/MS. The non-oxygenated sesquiterpenes α-copaene (21.15%) and α-gurjunene (13.69%), in addition to the oxygenated sesquiterpene spathulenol (10.32%), were detected as the majority constituents of the essential oil of the leaves, and a concentration similar to that of α-gurjunene was found in the branches (13.28%). The resin essential oil showed a high concentration of hydrocarbon monoterpenes (76.49%) with a predominance of α-pinene (17.57%) and limonene (46.11%). Four gall morphotypes were found associated with this species. The present study reports for the first time information on the volatile constituents and the resinous potential of P. aracouchini, and registers the morphotypes of the galls that help in the taxonomy of the species.

Protective Effect of Boswellic Resin Against Memory Loss and Alzheimer’s Induced by Aluminum Tetrachloride and D-Galactose (Experimental study in Mice)

Nowadays, because of the industrialization, a lot of contaminant were available ; the consequences of this availability are apparition of diseases including neurodegeneration. Neurodegenerative diseases of the human brain comprise a variety of disorders that affect an increasing percentage of the population. This study is based on the effect of the Boswellic resin, which is from a medicinal plant and known for its antioxidant effects on nerve cell damage. The objective of this work was to evaluate the in vitro and in vivo effects of the Boswellic resin on anticholinesterase activity and Alzheimer’s disease (AD) induced by D-galactose and aluminum tetrachloride in Swiss mice. Chemical composition of the resin essential oil was identified by the CG-MS analysis. The antioxidant activity was also assessed by the DMPD and metal chelation methods. In order to understand the mechanism of memory improvement, the acetylcholinesterase, AChE, and butyrylcholinesterase, BChE, inhibitory assays were performed. In vivo part of the study was achieved on Swiss mice divided into four groups: control, AD model, treated AD, and treated control group. The identification of chemical composition by CG-MS reach the 89.67% of the total extract compounds presented some very important molecules (p-Cymene, n-Octyl acetate, α-Pinene…). The present study proves that Boswellic resin improves memory and learning in treated Alzheimer’s group, modulates the oxidative stress and be involved in the protective effect against amyloid deposition and neurodegeneration, and stimulates the immune system in mice’s brain.

Population Status and Resin Quality of Frankincense Boswellia neglecta (Burseraceae) Growing in South Omo, Southwestern Ethiopia

ABSTRACT A study was conducted in South Omo Zone, Ethiopia with the aim of assessing the population status of the frankincense tree Boswellia neglecta and investigating its resin essential oil chemical composition. The status of populations of B. neglecta was assessed by examining the density, abundance, frequency, dominance, importance value index, and population structure. Resin sample was analyzed for the physicochemical properties. The composition of the essential oil was analyzed with Gas Chromatography-Mass Spectrometry. The high values of density, abundance, frequency, dominance, and importance value index for B. neglecta showed the potential of the tree for bulk resin collection. Boswellia neglecta had a bell-shaped diameter distribution indicating a hampered regeneration. The B. neglecta resin had a moisture content of 2.68%, ash content of 0.99%, pH of 5.7, and oil yield of 5.92%. The resin possessed good quality as compared to resins in other reports. The essential oil was optically active (−31.6° at 23.2°C). The essential oil contained several compounds, but 71.1% of the composition were formed mainly from methyl oleate, methyl linoleate, methyl palmitate, which have not been reported from B. neglecta. Sustainable management must be enacted since the agro-pastoralist mode of life hinders regeneration of the species and its resin resources.

Зміни вмісту монотерпенів живиці сосни у звʼязку з ураженням кореневою губкою (Heterobasidion annosum (Fr.) Bref.)

За допомогою газорідинної хроматографії виявлено тенденції змін вмісту монотерпенів у складі живиці дерев сосни IV класу віку, уражених кореневою губкою у різному ступені. Виявлено, що з розвитком хвороби вміст α-пінену і камфену знижується. Найменший їхній уміст, якщо порівняти зі здоровими деревами, виявлено у дерев, що знаходяться в активній зоні осередку всихання, ступінь ураження коріння яких становить 11–30 %. Уміст ∆3-карену в складі живиці цих дерев проти неуражених є, навпаки, вищим на 15–16 %. Уміст β-пінену в уражених кореневою губкою дерев був в 1,8–2,0 разу вищим, ніж у неуражених. Відзначено, що зміни в складі ефірних олій дерев в активній зоні осередку хвороби є сигналом щодо ослаблення дерева та можливості його заселення лубоїдами. Зміни в складі ефірних олій живиці в осередках кореневої губки провокують як патологічні процеси, так і зміни екології дерев унаслідок розвитку хвороби.

Chemical Composition of the Oleogum Resin Essential Oils of Boswellia dalzielii from Burkina Faso.

Frankincense, the oleogum resin from members of Boswellia, has been used as medicine and incense for thousands of years, and essential oils derived from frankincense are important articles of commerce today. A new source of frankincense resin, Boswellia dalzielii from West Africa has been presented as a new, alternative source of frankincense. In this work, the oleogum resins from 20 different Boswellia dalzielii trees growing in Burkina Faso, West Africa were collected. Hydrodistillation of the resins gave essential oils that were analyzed by GC-MS and GC-FID. The essential oils were dominated by α-pinene (21.0%–56.0%), followed by carvone (2.1%–5.4%) and α-copaene (1.8%–5.0%). Interestingly, there was one individual tree that, although rich in α-pinene (21.0%), also had a substantial concentration of myrcene (19.2%) and α-thujene (9.8%). In conclusion, the oleogum resin essential oil compositions of B. dalzielii, rich in α-pinene, are comparable in composition to other frankincense essential oils, including B. sacra, B. carteri, and B. frereana. Additionally, the differences in composition between samples from Burkina Faso and those from Nigeria are very slight. There is, however, a rare chemotype of B. dalzielii that is dominated by myrcene, found both in Burkina Faso as well as Nigeria.

Electrochemical detection of ursolic acid from spruce (Picea abies) essential oils using modified amperometric microsensors

Ursolic acid (UA) is a natural pentacyclic triterpenoid carboxylic acid found in some medicinal plant species. In this paper, amperometric microsensors based on a powder which contained graphite (G) and carbon nanoparticles (CN) (G-CN) unmodified and modified with chitosan (CHIT) and tetraphenyl-porphine cobalt(II) (Co(II)TPP) are proposed for the analysis of UA plant source essential oils obtained from spruce (Picea Abies). Differential pulse voltammetry (DPV) was used to optimize the method and for the determination of ursolic acid from different types of botanical samples. The optimum working pH was 5.00 for the G-CN and (Co(II)TPP)/G-CN microsensors and pH 3.00 for CHIT/G-CN in the presence of a 0.1 mol L−1 KCl supporting electrolyte. The linear concentration ranges for ursolic acid (UA) were between 0.1 and 100 µmol L−1 for the unmodified microsensor (G-CN), 0.01, 1 µmol L−1 for the microsensor modified with chitosan (CHIT/G-CN), and 0.01 and 10 µmol L−1 for the microsensor modified with (Co(II)TPP)/G-CN). It is the first time these amperometric microsensors have been used for the reliable analysis of ursolic acid (UA) in three original botanical samples obtained from different parts of spruce (Picea abies): resin essential oil, cons essential oil, cons and sprouts essential oil, with recovery rate values up to 99.29%.

Chemical Variation in Essential Oils from the Oleo-gum Resin of Boswellia carteri: A Preliminary Investigation.

Frankincense, the oleo-gum resin of Boswellia species, has been an important element of traditional medicine for thousands of years. Frankincense is still used for oral hygiene, to treat wounds, and for its calming effects. Different Boswellia species show different chemical profiles, and B. carteri, in particular, has shown wide variation in essential oil composition. In order to provide insight into the chemical variability in authentic B. carteri oleoresin samples, a hierarchical cluster analysis of 42 chemical compositions of B. carteri oleo-gum resin essential oils has revealed at least three different chemotypes, i) an α-pinene-rich chemotype, ii) an α-thujene-rich chemotype, and iii) a methoxydecane-rich chemotype.

Biological activity of Boswellia serrata Roxb. oleo gum resin essential oil: effects of extraction by supercritical carbon dioxide and traditional methods

ABSTRACTThe findings of this study suggests that chemical composition, essential oil yield, antioxidant and antimicrobial activity of Boswellia serrata oleo gum resin essential oils extracted by hydro distillation, steam distillation and supercritical fluid carbon dioxide methods vary greatly from each other. The optimum essential oil yield was obtained using hydro distillation method (8.18 ± 0.15 %). The essential oils isolated through different extraction methods contained remarkable amounts of total phenolics and total flavonoids. Essential oil isolated through supercritical fluid carbon dioxide extraction exhibited better antioxidant activity with highest free radical scavenging potential (96.16 ± 1.57 %), inhibition of linoleic acid oxidation (94.18 ± 1.47 %) and hydrogen peroxide free radical scavenging potential (68.25 ± 1.02 %). Moreover, the antimicrobial activity of essential oils was performed through well diffusion, resazurin microtiter plate and micro dilution broth assay assays. The essential oil isolated through steam distillation method revealed highest antimicrobial activity with maximum inhibition zone (24.21 ± 0.34 to12.08 ± 0.30 mm) and least MIC values (35.18 ± 0.77 to 281.46 ± 7.03 µg/mL). The comparison of chemical composition of essential oils isolated at different extraction methods have shown that the concentration of α-thujene, camphene, β-pinene, myrcene, limonene, m-cymene and cis-verbenol was higher in steam distilled essential oil as compared to hydro and supercritical fluid carbon dioxide extracted essential oils. These compounds may be responsible for the higher antimicrobial activity of Boswellia serrata oleo gum resin steam distilled essential oil.

Chemical Composition and Antibacterial Activity of the Essential Oil of Kauri Pine [ Agathis robusta (C. Moore ex F. Muell.) F.M. Bailey] from India

Agathis robusta (C. Moore ex F. Muell.) F.M. Bailey, commonly known as Queensland Kauri or smooth-barked Kauri, is a coniferous tree in the family Araucariaceae. This study aimed to investigate the chemical composition and antibacterial activity of the essential oil of A. robusta from India. The hydrodistilled resin and leaf essential oils were analyzed using GC-FID and GC-MS. Altogether, 34 constituents (forming 98.2% of the resin oil) and 43 constituents (forming 91.2% of the leaf oil composition) were identified. Major constituents of the resin oil were isobornyl acetate (37.9%), limonene (12.3%), bornyl acetate (7.4%), and myrtenol (5.8%), while the leaf oil contained β-selinene (18.1%), rimuene (14.2%), caryophyllene oxide (11.5%), spathulenol (10.5%), α-selinene (9.8%), and γ-muurolene (5.8%) as main constituents. The resin essential oil showed minimum inhibitory concentration (MIC) in the range of 250–500 μg/mL and minimum bactericidal concentration (MBC) in the range of 500 to >1000 μg/mL against pathogenic strains of eight bacteria.

Chemical Composition and in vitro Antibacterial Activity of the Essential Oils of the Leaves, Resin and Stem-Barks of Dacryodes edulis (G. Don) H. J Lam growing in Cameroon on Diarrhea Associated Strains

Chemical Constituents of the Essential Oil of Laggera pterodonta (DC.) Sch. Bip. From North-Central Nigeria Egharevba Henry Omoregie, Kunle Folashade Oluyemisi, Okwute Simon Koma, Okogun Joseph Ibumeh

Chemical composition, antimicrobial, insecticidal, phytotoxic and antioxidant activities of Mediterranean Pinus brutia and Pinus pinea resin essential oils

Essential oils of the resins of Pinus brutia and Pinus pinea were evaluated for their biological potential. Essential oils were characterized using GC-MS and GC/FID. in vitro antimicrobial, phytotoxic, antioxidant, and insecticidal activities were carried out using the direct contact and the fumigant assays, respectively. The chemical profile of the essential oils of the resins of P. pinea and P. brutia included mainly α-pinene (21.39% and 25.40%), β-pinene (9.68% and 9.69%), and caryophyllene (9.12% and 4.81%). The essential oils of P. pinea and P. brutia exerted notable antimicrobial activities on Micrococcus luteus and Bacillus subtilis, insecticidal activities on Ephestia kuehniella eggs, phytotoxic activities on Lactuca sativa, Lepidium sativum, and Portulaca oleracea, as well as antioxidant potential. Indications of the biological activities of the essential oils suggest their use in the formulation of ecofriendly and biocompatible pharmaceuticals.

Chemical composition and antibacterial activity of foliage and resin essential oils of Araucaria cunninghamii Aiton ex D.Don and Araucaria heterophylla (Salisb.) Franco from India

The essential oils of foliage and resin of Araucaria cunninghamii Aiton ex D.Don and Araucaria heterophylla (Salisb.) Franco were isolated by hydrodistillation and subsequently analyzed using gas chromatography (GC/FID) and GC–mass spectrometry (GC/MS). Altogether 113 constituents, representing 84.0–97.6% of the total oil compositions were identified. Major constituents of the foliage oil of A. cunninghamii were beyerene (34.6–44.4%), caryophyllene oxide (0.5–17.9%), α-pinene (3.3–16.2%), germacrene D (0.1–9.8%), kaurene (1.7–5.1%), and 13-epi-dolabradiene (4.2–4.8%). However, the resin oil of A. cunninghamii was characterized by higher amounts of (E)-caryophyllene (60.8%), caryophyllene oxide (13.4%), and (E)-β-farnesene (4.9%). The foliage oil of A. heterophylla was dominated by 13-epi-dolabradiene (42.7%), beyerene (22.2%), rimuene (13.7%), and dolabradiene (3.9%), whereas the resin oil of A. heterophylla contained α-copaene (29.9%), germacrene D (21.4%), γ-gurjunene (9.7%), δ-cadinene (7.1%), and sandaracopimara-8(14),15-diene (6.5%) as main constituents. The foliage and resin essential oils of both species showed minimum inhibitory concentration (MIC) in the range of 250–500μg/mL and minimum bactericidal concentration (MBC) in the range of 1000 to >1000μg/mL against tested bacterial strains.

Antifungal and Antibacterial activities of Aucoumea klaineana Pierre Essential Oil From Gabon

Aucoumea klaineana Pierre (Burseraceae) is largely distributed in equatorial forest from Gabon to Equatorial Guinea. It is a medicinal plant that resin, roots and leaves are used to treat fever, constipation, malaria, diarrhea, jaundice and sexual transmissible infection treated by traditional healers. The predominant constituents in the essential oil were δ-3-carene. The antimicrobial activities of both essential oils were tested against Gram-positive and Gram -negative bacteria by using agar disc diffusion and broth microdilution methods; anticandidal effect was also tested on different strains of Candida albicans. The essential oil exhibited antibacterial and antifungal activities against the all strains tested. The best inhibition zones were obtained for Shigella dysenteria CIP 5451 and for Candida albicans ATCC90028. The results suggest that Aucoumea klaineana resin essential oil could be a natural antimicrobial agent.

LARVICIDAL EFFECT OF EUCALYPTUS GRANDIS ESSENTIAL OIL AND TURPENTINE AND THEIR MAJOR COMPONENTS ON AEDES AEGYPTI LARVAE

In the search for new alternatives for the control of Aedes aegypti the larvicidal activity of Eucalyptus grandis essential oil and pine resin essential oil (turpentine) and their major components (alpha- and beta-pinene and 1,8-cineole) was determined. Gas chromatography-mass spectroscopy analysis of E. grandis essential oil revealed that its major components are alpha-pinene and 1,8-cineole. Similar analysis of turpentine obtained by distillation of the resin pitch of conifers showed that alpha- and beta-pinene are the only major components. Third and early 4th instars of the CIPEIN-susceptible strain of Ae. aegypti were exposed to acetonic solutions of E. grandis essential oil, turpentine, and their major components for 24 h. Turpentine, with an LC50 of 14.7 ppm, was more active than the essential oil of E. grandis (LC50: 32.4 ppm). Larvicidal activity of the essential oil components showed that alpha- and beta-pinene present low LC50 values (15.4 and 12.1 ppm, respectively), whereas pure 1,8-cineole showed an LC50 of 57.2 ppm. These results suggest that alpha-pinene in E. grandis and alpha- and beta-pinene in turpentine serve as the principal larvicidal components of both oils. Results obtained on larvicidal effects of essential oil of Eucalyptus grandis and turpentine could be considered a contribution to the search for new biodegradable larvicides of natural origin.