This paper investigates the effect of steel reinforcement configured with different strengths after different high temperatures on the shear performance of the test beams, a total of 20 reinforced concrete (RC) beams, through experiments, numerical analyses and theoretical studies, in order to understand the key factors affecting the shear performance of the beams. The test revealed that the mechanical properties of concrete and steel reinforcement showed a trend of increasing and then decreasing after high temperatures. Increasing the stirrup ratio and stirrup strength can significantly improve the crack resistance and shear capacity of the beams, as well as the deformation performance of the beams. The residual shear strengths of test beams with high-strength stirrups after exposure to high temperatures are significantly greater than those of ordinary reinforced concrete beams. Specifically, the shear capacity of beams with high-strength stirrups after exposure to 800°C increased by 23.7 % compared to ordinary beams, and the mid-span deflection performance increased by 28.7 %. The stresses in the shear span region of the test beams after high temperature were more concentrated at the diagonal and the number of cracks was significantly reduced. In this paper, a simplified formula for calculating the residual shear capacity of RC beams after room and high temperatures is proposed, which can well predict the test results.