Sequencing centres differ not only in terms of their scale and output, but also their requirements for information management. Sequencing platforms are becoming more accessible, and the efficient storage of genomic metadata is vital for large and small sequencing centres alike. Off-the-shelf solutions are often very expensive and not cost-effective for the smaller centre. Furthermore, support contracts are often required, and the extensibility of these systems is not in the hands of the metadata generators. In terms of implementation, as well as the desire to tailor an information system in-house, data formats can change and platforms can evolve rapidly. These are valid concerns for both large centres characterised by high-throughout data production and smaller scale laboratories with constrained expenditure for IT solutions, and potentially project specific metadata requirements. Hence, we are developing MISO, an open-source LIMS for recording sequencing metadata. We are using freely available tools that are industry standard, well documented, and easy to set up on minimal hardware. As a bare system, MISO can store relevant metadata based on the most common sequencing platforms (e.g. Illumina GA and HiSeq, Roche 454 and ABI SOLiD) and public repository data submission schemas (e.g. the Sequence Read Archive at the EBI), and has many features common to bespoke and proprietary LIMS, such as secure authentication, fine-grained access control, barcode tracking, and reporting. Features MISO accomplishes all of these elements: ● Authentication – user-centric access control to designated areas ● Laboratory tracking – project description, sample receipt, library preparation, run construction, and barcoding ● Printing – can directly connect to barcode printers and print barcodes for the relevant objects inside MISO ● Bioinformatics pipelines – monitoring and reporting of analytical processes, interacting directly with the computing cluster ● Reporting – accurate statistics from library preparation and sequencing processes (Figure 1) ● Data Visualisation – through “traffic light” indicators and treestructured status diagrams, MISO can easily show the status of each project and its elements (Figure 1) ● Submission – automated packaging of sequencing data and metadata, deployment of these data to remote public repositories ● Notification – automated import of run metadata and notification of change of run status ● SRA – MISO is heavily modelled upon the submission schemas specified by the SRA (http://www.ebi.ac.uk/ena/) and is therefore able to automatically generate and deploy the required XML and data files (Figure 2) We are working on new plugin features to enable MISO to be extendable, making it easier for the community to contribute more features.