Reproductive Anatomy Research Articles

Reproductive Systems, Transfer and Digestion of Spermatophores in Two Asian Luciolinae Fireflies (Coleoptera: Lampyridae).

Simple SummaryDuring mating, fireflies may transfer sperm to the female wrapped in food materials (spermatophores). We investigate in two firefly species structures in both male and female that indicate production and receipt of spermatophores. Their structure, how they attach and discharge the sperm inside the female, and how long they persist might indicate if these fireflies mate only once or more. Potential differences between males and females of both fully winged and species with flightless females are determined. An overview of present knowledge of female reproductive anatomy is given. An argument is mounted suggesting direct observation of biological structures like spermatophores might be the only reliable way of determining their presence.The internal reproductive anatomy of males and females of two Asian Luciolinae fireflies Emeia pseudosauteri (Geisthardt, 2004) and Abscondita chinensis (L., 1767) is described, and the time course for spermatophore transfer and digestion examined. E. pseudosauteri is sexually dimorphic, with a flightless female, and Abs. chinensis is sexually monomorphic, with the female flighted. Both are monandrous. Possible female accessory glands are described for the first time for both species. An overview of present knowledge of female reproductive anatomy in the Luciolinae reveals males of 18 species in 10 genera may produce spermatophores and permits speculation about spermatophore production in another 16 genera.

Options for acquiring motherhood in absolute uterine factor infertility; adoption, surrogacy and uterine transplantation.

Key content Following the diagnosis of absolute uterine factor infertility (AUFI), women may experience considerable psychological harm as a result of a loss of reproductive function and the realisation of permanent and irreversible infertility.Adoption enables women with AUFI, and their partners, to experience social and legal parenthood, also often providing benefits for the adopted child.Surrogacy offers the opportunity to have genetically related offspring. Outcomes are generally positive in both surrogates and the children born as a result.Uterine transplantation is the only option to restore reproductive anatomy and functionality. While associated with considerable risk, it allows the experience of gestation and the achievement of biological, social and legal parenthood. Learning objectives To gain an understanding of the routes to parenthood available for women with AUFI experiencing involuntary childlessness, such as adoption, surrogacy and, most recently, uterine transplantationTo consider a suggested management plan to facilitate counselling in women with AUFI who experience involuntary childlessness. Ethical issues In the UK, while the number of children requiring adoption continues to increase, the number being adopted from care is decreasing.Some cultures may hold ethical or religious beliefs that surrogacy is unacceptable, and its legal position in many jurisdictions is problematic.Restrictive selection criteria and high costs may limit future availability of uterine transplantation

Food attractants for field population monitoring of Spodoptera exigua (Hübner)

Spodoptera exigua (Hübner) is an important agricultural pest with worldwide distribution. Monitoring the population dynamics of adults and predicting the occurrence of next generation are the basis of prevention and control. Insect attractants are based on preferences for foods or their volatiles and have great potential for pest monitoring and management. To evaluate the monitoring success of food attractants on S. exigua populations in the field, we first quantitatively analyzed the reproductive organ development of adults in a laboratory population. The ovarian development of females was divided into four stages. The length range of the testis major axis of males at different ages was established according to the principle of testis development. This allowed documentation of the reproductive status of adults trapped in the field. In 2019 and 2020, we used food attractant trapping combined with reproductive system examination to monitor the population dynamics and reproductive development of S. exigua adults in natural environment. The results showed obvious generational changes in the population density dynamics of males and females of S. exigua. During the two-year monitoring period, the ovarian development level and mating times of trapped females in the second generation increased at first and then decreased with time. The number of eggs within ovarioles trapped initially decreased and then increased over time. The ovarian development level and mating times of trapped females in the third-generation adults increased gradually over time, and the number of eggs in female ovarioles decreased gradually over time. The age of trapped males in the second generation initially increased and then decreased over time. The age of males in the third generation increased gradually over time. There were significant differences in the reproductive development of female and male moths trapped in different generations in 2019. This study showed that trapping using food attractants can monitor field population dynamics of S. exigua. Combined with examination of reproductive system anatomy, it can monitor the reproductive organ development, mating, and oviposition status of wild adults.

Computational in Silico Modelling of Phytochemicals as a Potential Cure

Urinary tract infection (UTI) is one of the most severe public health problems affecting both sexes but females are more susceptible due to the differences in urogenital and reproductive anatomy, physiology and lifestyle. Due to multi?drug resistant strains and high recurrence rate, UTI has become a major socioeconomic burden. It was found that microbial infections including Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa and Proteus mirabilis species are the major causes of UTI with different signs and symptoms including painful urination or dysuria, haematuria, urinary urgency, burning micturition, frequent urination, nausea, and vomiting. Phytochemicals are effective to combat bacterial resistance with high efficacy, and easy availability with minimal or no side effects. For this reason, we present a docking?based screening using a quantum mechanical scoring of a library built from approved drugs and compounds that Curcumin, Anolignan B, Piperine, Carvacrol, Quercetin, Kaempferol, Citral, Allyl Isothiocyanate with Proteins with PDB id’s 2N50, 4C4V, 6H1X, 4UU4 could display antibacterial activity against UTI. Clearly, these compounds should be further evaluated in experimental assays and clinical trials to confirm their actual activity against the disease. We hope that these findings may contribute to the rational drug design against UTI.

Artificial insemination of cattle: Description and assessment of a training program for veterinary students

The objective was to assess the effectiveness of a comprehensive artificial insemination (AI) training program designed to facilitate an understanding of the breadth of the AI process, including AI skill acquisition, for preclinical veterinary students. Participants (n = 303) were enrolled at the Ross University School of Veterinary Medicine (Basseterre, St. Kitts, West Indies). The 2-d AI training program (n = 20) consisted of ∼8 h of instruction and ∼8 h of demonstration and hands-on activity. Oral presentations were used to deliver educational content, followed by video clips, discussion, demonstrations, and hands-on activity. Reproductive anatomy and physiology of the estrous cycle, AI sire acquisition, collection, evaluation, cryopreservation and distribution of conventional and sexed semen, storage and handling of frozen semen, use of synchronization protocols, accurate and efficient detection of estrus, and correct AI technique were discussed. True or false pre- and posttests were used to determine the level of knowledge gained by participants during the AI training program. Preclinical veterinary students were required to complete a semen handling and AI technique practical exam to achieve a certificate of completion. Participant program evaluations conducted at the conclusion of the program indicated that veterinary students found the content, structure, discussion, demonstrations, and hands-on activities to be appropriate and useful. No negative comments were offered about the training program, instructor, or activity coinstructors. The AI training program increased the posttest knowledge scores of veterinary students by 22 percentage points. Only 1 participant was unable to achieve a certificate of completion due to failure of the semen handling and AI technique practical exam. These results provide evidence that the AI training program was relevant and effective and that it offered information and skill acquisition with immediate field application.

Correlates of reproductive tract anatomy and uterine histomorphometrics with fertility in swine

Economic potential of the swine industry hinges upon the reproductive performance of sows, which may be enhanced by improving uterine capacity, a component trait of litter size and piglet productivity. Previous attempts at characterizing morphological traits indicative of high uterine volume have not been completely successful, resulting in the continued need for a reliable method of predicting reproductive value to improve production efficiency of the sow. Hence, the main objective of this study was to scrutinize macro- and micro-morphology of the sow’s reproductive tract for quantitative correlations with fertility indices. Reproductive records from Polish Landrace × Polish Large White sows were used to examine the associations between fertility and ovarian/uterine morphology (n = 34) or uterine histomorphometry (n = 10). Several measures related to the ovary, including right and left ovarian weight (r = 0.50, p = 0.005 and r = 0.49, p = 0.006, respectively), were positively correlated with the litter size, while left ovarian number of corpora lutea (r = −0.38, p = 0.04) was negatively correlated with the mean litter size. Analysis of histomorphological characteristics of the uterine wall collected during the luteal phase of the estrous cycle revealed correlations between mean litter size and myometrial vascular content (r = 0.75, p = 0.03), the proportion of myometrial stroma (r = −0.68, p = 0.03), and the variability of endometrial thickness (r = −0.72, p = 0.02) in sows. Eight ovarian, vaginal and uterine characteristics were significantly correlated with mean lifetime numbers of live born and stillborn piglets/litter or the last litter size before slaughter. In conclusion, several anatomical and histomorphological metrics that relate to reproductive performance of swine may be used to inform production protocols and as a tool for selection of elite breeding sows, warranting future research into non-invasive or minimally invasive techniques for obtaining such measures.

Developing a visual aid to improve women’s knowledge of hysterectomy

BackgroundFemale reproductive anatomy and physiology, compounded by low health literacy, can result in limited patient understanding of gynecologic procedures, complicating the already challenging patient decision-making process. ObjectiveDescribe a process of developing an effective visual aid that accurately communicates the risks, benefits, and possible outcomes of hysterectomy surgical approaches. Patient InvolvementNone. MethodsThis qualitative study was performed in a private practice and an academic institution with a total of 8 female non-clinical employees in the English-speaking group and 9 non-employees in the Spanish-speaking group. A 5-minute video focusing on the hysterectomy procedure was developed and shown to focus group participants. A discussion led by a sociologist obtained feedback regarding understanding and perceptions of video content to validate the tool, which was analyzed to extract themes that could guide refinements. ResultsFocus group participants wanted more information on post-hysterectomy quality of life and desired a longer video that included more general information about hysterectomies. All participants felt the video would be a valuable aid if watched prior to undergoing a hysterectomy and would be an effective tool for improving patient communication. DiscussionWhile there was strong support for the video-based patient education program, participants found some elements confusing (e.g., type and magnitude of risk, scope of aftercare) and expressed interest in more in-depth information. Our development process would have been enhanced by involving patients before the initial version of the video was created, holding more and more diverse focus groups and ensuring that translation was both accurate and culturally appropriate. Practical ValueThis study offers lessons learned in the process of developing a video-based visual aid for improving women's knowledge of hysterectomy and may inform other efforts to help patients understand complex medical pathology and procedures.

Genotypic variations in reproductive morphology and anatomy of hot chilli (Capsicum chinense Jacq.) grown under elevated CO2 and temperature

Genotypic variations in reproductive morphology and anatomy of hot chilli (Capsicum chinense Jacq.) grown under elevated CO2 and temperature

43 Correlates of reproductive tract anatomy and uterine histomorphometrics with fertility in swine

Economic potential of the swine industry hinges upon the reproductive performance of sows, which may be enhanced by improving uterine capacity, a component trait of litter size and piglet productivity. Previous attempts at characterising morphological traits indicative of high uterine volume have not been completely successful, resulting in the continued need for a reliable method of predicting reproductive value to improve production efficiency of the sow. Hence, the main objective of this study was to scrutinize macro- and micro-morphology of the sow’s reproductive tract for quantitative correlations with fertility indices. Reproductive records from Polish Landrace×Polish Large White sows (mean±s.e.m. parity: 4.3±0.6, range: 2–8) were used to examine the associations between fertility and ovarian/uterine morphology (n=34) or uterine histomorphometry (n=10) posthumously. Simple linear regression was performed to determine the relationship between anatomical or histological parameters and various measures of reproductive performance. Several measures related to the ovary, including right and left ovarian weight (r=0.50, P=0.005, and r=0.49, P=0.006, respectively), were positively correlated with the litter size, whereas left ovarian number of corpora lutea (r=−0.38, P=0.04) was negatively correlated with the mean litter size. Analysis of histomorphological characteristics of the uterine wall collected during the luteal phase of the oestrous cycle revealed correlations between mean litter size and myometrial vascular content (r=0.75, P=0.03), the proportion of myometrial stroma (r=−0.68, P=0.03), and the variability of endometrial thickness (r=−0.72, P=0.02) in sows. Eight ovarian, vaginal, and uterine characteristics were significantly correlated with mean lifetime numbers of live born and stillborn piglets/litter or the last litter size before slaughter. In conclusion, several anatomical and histomorphological metrics that relate to reproductive performance of swine may be used to inform production protocols and as a tool for selection of elite breeding sows, warranting future research into noninvasive or minimally invasive techniques for obtaining such measures.

The Evolution of Viviparity in Vertebrates.

In the vertebrate tree of life, viviparity or live birth has independently evolved many times, resulting in a rich diversity of reproductive strategies. Viviparity is believed to be a mode of reproduction that evolved from the ancestral condition of oviparity or egg laying, where most of the fetal development occurs outside the body. Today, there is not a simple model of parity transition to explain this species-specific divergence in modes of reproduction. Most evidence points to a gradual series of evolutionary adaptations that account for this phenomenon of reproduction, elegantly displayed by various viviparous squamates that exhibit placentae formed by the appositions of maternal and embryonic tissues, which share significant homology with the tissues that form the placenta in therian mammals. In an era where the genomes of many vertebrate species are becoming available, studies are now exploring the molecular basis of this transition from oviparity to viviparity, and in some rare instances its possible reversibility, such as the Australian three-toed skink (Saiphos equalis). In contrast to the parity diversity in squamates, mammals are viviparous with the notable exception of the egg-laying monotremes. Advancing computational tools coupled with increasing genome availability across species that utilize different reproductive strategies promise to reveal the molecular underpinnings of the ancestral transition of oviparity to viviparity. As a result, the dramatic changes in reproductive physiology and anatomy that accompany these parity changes can be reinterpreted. This chapter will briefly explore the vertebrate modes of reproduction using a phylogenetic framework and where possible highlight the role of potential candidate genes that may help explain the polygenic origins of live birth.

Corrigendum to: 43 Correlates of reproductive tract anatomy and uterine histomorphometrics with fertility in swine.

Corrigendum to: 43 Correlates of reproductive tract anatomy and uterine histomorphometrics with fertility in swine.

The Embryonic re-Development of an Anatomy Museum : Visualising Anatomical Collections: Human Female Reproductive Anatomy and Biology and Foetal Development.

This chapter discusses the history of the Museum of Anatomy at the University of Glasgow in the context of a planned themed display on obstetrics and pregnancy, centred around human female reproductive anatomy, to support a showcase of Plaster Casts made and used by William Hunter. This exhibition aims to enhance the audience's experience with an educational display of historical specimens as well as anatomical artwork and medical models. It is anticipated that the resultant exhibition will include a series of visualisations and diagrams for use within the collection display to support the audience's understanding of the biological processes involved in reproduction, foetal development and women's experiences of childbirth. The chapter considers historical and contemporary methods of visualising embryos, as well as the developing discourse around menstruation, the gendered body and thelack of diverse representation in gynaecological images, and reflects on some of the historical, scientific, situational and societal considerations needed to achieve an inclusive and accessible exhibition. It also reflects on the artist's role in theembryonic development of thisexhibition. The artworks in this chapter and the more that are planned should guide viewers with intentionally inclusive visual content. The project requires considerable further development and discussion with the team of experts involved. It is hoped that this intervention will broaden the impact of the collections in this space and provide opportunities to improve audience engagement by creating content that reflects and includes the voices of society in its creation.

Diagnostic Hysterolaparoscopy in the Evaluation of Female Factor Infertility: A Cross-sectional Study at a Tertiary Care Hospital in Central India

ABSTRACT Background Infertility is a worldwide problem affecting people of all geographical location and socioeconomic status. It is estimated that globally 60–80 million couples suffer from infertility every year. The main objective of the study is to detect the diagnostic efficacy of hysterolaparoscopy in evaluating the tubal, uterine, ovarian, and pelvic pathologies causing infertility and also compare the findings of a hysterosalpingogram with diagnostic laparoscopy in evaluating the tubal factor infertility. Materials and methods This hospital-based cross-sectional study included an evaluation of 1,456 couples presenting with complaints of infertility at the Outpatient Department of Government Medical College, Nagpur during the period of February 2016 to September 2018. After thorough gynecological examination and investigations, 130 infertile women were posted for diagnostic hysterolaparoscopy. All the data were collected on predesigned proforma and the results were tabulated and raw percentages calculated to describe the results. Results Among the 130 infertile women, 102 women (78.46%) had primary and 28 (21.53%) had secondary infertility. The maximum number of patients belonged to the age group of 26–30 years (39.23%). About 56% of patients were in the active marriage life of 3–5 years duration. Most of them had multiple factors affecting their fertility. Out of the 130 infertile women, 32 patients (24.62%) had uterine factors, 44 patients (33.84%) had peritoneal factors, 67 patients (51.5%) had ovarian factors, and 89 patients (68.46%) had tubal factors. Only eight patients (6.15%) had normal findings in laparoscopy. Out of the 130 patients, 122 (93.84%) had more than one factor causing infertility. About 26.15% of patients had positive findings in diagnostic hysteroscopy. Conclusion Hysterolaparoscopy provides both a panoramic view of the pelvic reproductive anatomy and also a magnified view of the pelvic organs and peritoneal surfaces. It is generally regarded as the most reliable tool in the diagnosis of tubal pathology and other intra-abdominal causes of infertility because of the correctable abnormalities like endometriosis, adnexal adhesions, and septate uterus which are usually missed, are usually identified that helps in formulating a specific plan of management. How to cite this article Selvaraj P, Parpillewar M, Fidvi J. Diagnostic Hysterolaparoscopy in the Evaluation of Female Factor Infertility: A Cross-sectional Study at a Tertiary Care Hospital in Central India. Int J Infertil Fetal Med 2020;11(2):37–41.

The learning continuum of living reproduction: Generating a curriculum grid based on students' cognitive levels

One of the overlapping curriculum designs indicates the mismatch of teaching materials with the cognitive level process and student development, impacting the unstructured qualification of mastery of the material. On the other hand, the breadth and depth of teaching materials in the curriculum must be following students' mental development and level of cognitive processes. This study aims to collect the opinions of science teachers in junior high schools and biology teachers in high schools about the learning continuum design on reproducing living things. The method used is a survey. The study population is a hypothetical population with a convenience sample. The sample involved in this study were 111 teachers in Bantul Regency and Yogyakarta City. The data collection method used a questionnaire distributed through four subject teachers' conference (MGMP). The data obtained were analyzed using descriptive analysis. The results showed several sub-aspects of reproduction of living things that cannot be taught in elementary schools. The material for propagating fungi, protists, bacteria, and viruses starts in grade VII with a cognitive level of C1 (remembering), after previously students received material about types of animals and plants in grade VI (elementary school). Furthermore, the material on reproductive anatomy and physiology can only be given to class IX with cognitive level C2 (understanding) and continued in class X and XI with cognitive level C4 (analyzing). Especially for reproductive anatomy and physiology, fungi were introduced to class VII with C1 level cognitive processes (remembering). This teacher opinion generates a learning continuum grid that can help policymakers improve educational curriculum.

73 Engaging youth through bovine reproductive practices

Abstract Two programs have been established at Virginia Tech with the purpose of engaging youth and showcasing agriculture. The Governor’s School for Agriculture (GSA) program began in 2001, and has over 1,850 graduates. High school juniors and seniors from different areas of the Commonwealth’s schools spend time on campus exploring the life sciences. The Virginia 4-H State Congress (4HC) is the premier, statewide annual 4-H event for outstanding teens, providing educational experiences to Virginia teens and helping them develop life skills and leadership abilities. Our extension group has focused on exposing teens enrolled in the GSA and 4HC to beef production, particularly bovine reproduction and reproductive biotechnologies. The 4-hr workshop, titled “Do it yourself cows”, consists of a mixture of in-class presentations and hands-on laboratories relating to bovine reproductive anatomy and physiology and reproductive biotechnologies. The wet-laboratory consists of bovine female reproductive tracts anatomy dissection and artificial insemination and rectal palpation simulation with a mannequin, as well as demonstrations of equipment used in the field including, nitrogen tank for semen storage, ultrasound and microscope visualization of bovine embryos. The GSA “Bovine Reproduction” course is 4 days long and allows for in-depth discussion of factors influencing fertility and case discussions on beef reproductive management. The hands-on portion of this course is similar to the 4HC workshop, however students are able to perform rectal palpation and ultrasonography in cattle. So far, 40 students from the GSA and 80 students from the 4HC (2 GSA, and 4 4HC programs) have graduated in the course. At the completion of both programs, a survey was performed and 100% of the students considered the program positive and gained knowledge and interest in bovine reproduction. Furthermore, 20% of students from the GSA program developed a desire to pursue a career in livestock production, and 92% of 4HC students claimed to have learned information they can apply in their communities. Our data indicate that we were successful in engaging youth in animal agriculture, specifically beef production.

Honeybush (Cyclopia spp.) anther-stigma distance and intraspecies cross compatibilities

Species of Cyclopia are used to produce honeybush tea, a uniquely South African beverage that is rapidly growing in popularity locally and abroad. In response to warnings about ecological sustainability, the honeybush industry is transitioning from a wild-harvested source model to one relying primarily on commercial farming. To this end, honeybush genotypes are needed with improved homogeneity, sensory qualities, productivity, vigour and resistance to such pest and disease conditions as may arise in massed plantings. In support of existing honeybush breeding programmes, studies have been undertaken to answer pressing questions about honeybush reproductive anatomy and sexual compatibility. Anther-stigma distance was measured within and between honeybush genotypes in order to rule out flower heteromorphism as a cause for low seed set following hand pollination. Continuous variation in anther-stigma distance was observed for the homomorphic, leguminous flowers, suggesting the existence of a mixed-mating system. Diallel crosses were then performed by hand pollination between genotypes within three honeybush species. All three species were found to be partially self-compatible, with large variations in self-incompatibility between genotypes within species. This study provides practical recommendations with regard to the sexual propagation of select honeybush species and expands the platform of scientific research available to honeybush breeders.

Descriptions of four new dextral land snails of the genus Camaena (Gastropoda, Eupulmonata, Camaenidae) from south China.

In this study, four new dextral camaenid from China are reported, based on shell morphology, reproductive system anatomy, and molecular phylogenetic analyses: Camaenafuningensis Zhou, Wang & Lin, sp. nov., Camaenagaolongensis Zhou, Wang & Lin, sp. nov., Camaenamaguanensis Zhou, Wang & Hu, sp. nov., and Camaenayulinensis Zhou, Wang & Hu, sp. nov. Detailed descriptions of the morphological characteristics including shells and genitalia, DNA sequences, and living environments of the four new species are provided, with further comparisons with congeners.

Pediatric granular cell tumor of vulva: A rare benign tumor

We present a rare case of a school-aged girl with granular cell tumor of the vulva. The patient presented with a right labial swelling of unknown duration. Initial diagnostic workup with ultrasound suggested a gonadal structure in the right labia, which was read as ovarian herniation through the Canal of Nuck into the right labia majora. Given concerns for a concurrent Mullerian dysgenesis syndrome predisposing the patient to ovarian herniation, the patient was scheduled for a diagnostic laparoscopy and hernia repair with possible excision of vulvar mass. Laparoscopy revealed normal reproductive anatomy with two visualized normal appearing pelvic ovaries. Subsequent excision of the mass was determined to be granular cell tumor by pathology. There are fewer than 20 reported cases of pediatric vulvar granular cell tumors in the literature and no reports of granular cell tumors presenting as ovarian structure on imaging. This case highlights the importance of including this rare tumor in the differential diagnosis for vulvar mass which may be misread as ovary on ultrasound. • Granular cell tumors of the vulva are extremely rare, especially in children. • Ultrasound imaging of the tumor may present as ovarian structures. • Clinicians should include granular cell tumors within the differential for a vulvar mass. • In children wide local excision is curative and follow up is necessary for minimum of two years due to high recurrence rates.

A Review of the Ecomorphology of Pinnotherine Pea Crabs (Brachyura: Pinnotheridae), with an Updated List of Symbiont-Host Associations

Almost all pea crab species in the subfamily Pinnotherinae (Decapoda: Brachyura: Pinnotheridae) are considered obligatory endo- or ectosymbionts, living in a mutualistic or parasitic relationship with a wide variety of invertebrate hosts, including bivalves, gastropods, echinoids, holothurians, and ascidians. While the subfamily is regarded as one of the most morphologically adapted groups of symbiotic crabs, the functionality of these adaptations in relation to their lifestyles has not been reviewed before. Available information on the ecomorphological adaptations of various pinnotherine crab species and their functionality was compiled in order to clarify their ecological diversity. These include the size, shape, and ornamentations of the carapace, the frontal appendages and mouthparts, the cheliped morphology, the ambulatory legs, and the reproductive anatomy and larval characters. The phylogenetic relevance of the adaptations is also reviewed and suggestions for future studies are made. Based on an updated list of all known pinnotherine symbiont–host associations and the available phylogenetic reconstructions, it is concluded that, due to convergent evolution, unrelated species with a similar host interaction might display the same morphological adaptations.

Attitudes toward fertility preservation among transgender youth and their parents

Abstract Purpose While gender-affirming hormones (GAH) may impact the fertility of transgender and gender diverse (TGGD) youth, few pursue fertility preservation (FP). The objective of this study is to understand youth and parent attitudes toward FP decision-making. Methods This study is a cross-sectional survey of youth and parents in a pediatric, hospital-based gender clinic from April to December 2017. Surveys were administered electronically, containing 34 items for youth and 31 items for parents regarding desire for biological children, willingness to delay GAH for FP, and factors influencing FP decisions. Results The mean age of youth (n = 64) was 16.8 years, and 64% assigned female at birth; 46 parents participated. Few youth (20%) and parents (13%) found it important to have biological children or grandchildren, and 3% of youth and 33% of parents would be willing to delay GAH for FP. The most common factor influencing youth FP decision-making was discomfort with a body part they do not identify with (69%), and for the parents, whether it was important to their child (61%). In paired analyses, youth and their parents answered similarly regarding youth desire for biological children and willingness to delay GAH for FP. Conclusions The majority of TGGD youth and parents did not find having biological offspring important and were not willing to delay GAH for FP. Discomfort with reproductive anatomy was a major influencing factor for youth FP decision-making and their child's wishes was a major factor for parents. Future qualitative research is needed to understand TGGD youth and parent attitudes toward FP and to develop shared decision-making tools.