Repeated Measures Design Research Articles

Greenwashing in food labelling: Consumer deception by claims of climate neutrality and the importance of an interpretative labelling approach

Approaches to climate labelling are gaining significant importance in food marketing. Labels that are based on carbon offsets are becoming increasingly popular. However, offsetting-labels have been criticized as misleading and are subject to accusations of greenwashing. The proposal of the EU Green Claims Directive therefore requires companies to substantiate claims. This study employs a consumer survey (n = 2,109) using a between-subjects and within-subjects design to explore how German participants evaluate the climate impact of six food products (ranging from low to very high) through five distinct climate labels: (1) ‘climate-neutral’ (without any declaration), (2) ‘climate-compensated’, (3) ‘climate-neutral and CO2-compensated product’ (declaration according to the proposed EU Green Claims Directive), (4) informative labels indicating the actual climate impact as a numeric carbon footprint (kg CO2eq/kg of food) or (5) as an interpretative traffic light-like label. Except for the numeric indication of the carbon footprint (4) and the traffic light (5), all climate labels significantly skewed perceptions of a food’s climate impact positively, compared to the control group without any label. The effect was sometimes even stronger for highly involved consumers. In contrast, the interpretative traffic light climate label helps to correctly assess the climate impact. In summary, green claims such as ‘climate-neutral’ can be misleading by fostering a false perception of a food’s climate impact, even when the compensatory character is explained or justified close to the claim. This challenges the approach of the draft European Green Claims Directive, which posits that additional information (‘substantiation’) is sufficient to avoid misconceptions.

Acute analgesic effect of nicotine vaping using three experimental pain induction tasks: a randomized, placebo-controlled laboratory study.

Pain and nicotine use are co-occurring conditions with a significant impact on health. Experimental evidence supports an acute analgesic effect of nicotine which may reinforce nicotine use among those with chronic pain. Evidence for nicotine analgesia have primarily been gathered in combustible cigarette users and have not been extended to electronic nicotine delivery systems (ENDS or vaping). Furthermore, the mechanisms of nicotine analgesia in humans are not well understood. Assess the effect of acute vaped nicotine on subjective and behavioral indices of pain sensitivity using three tasks designed to probe distinct mechanisms of analgesia. This study recruited ENDS users (N = 86) to undergo a paced vaping protocol followed by pain tasks in counterbalanced order. Across four sessions, participants vaped e-liquid containing nicotine or placebo, and flavor or no-flavor in a 2 × 2 within-subject design. Assessments included cold pressor, submaximal effort tourniquet to induce ischemic pain, and temporal summation of heat pain, an index of central sensitization. Compared to placebo, nicotine increased cold pressor pain tolerance (ηp2 = 0.031), ischemic pain threshold (ηp2 = 0.073) and tolerance (ηp2 = 0.056) but had no effect on temporal summation of pain. Flavor did not affect pain sensitivity. Females reported greater ischemic pain sensitivity (ηp2 = 0.027) and greater reductions in craving (ηp2 = 0.086). Consistent with research from tobacco smoking, analgesia may be reinforcing and contribute to nicotine dependence among ENDS users. More research on sex differences is warranted.

Machine learning approaches to predict whether MEPs can be elicited via TMS

BackgroundTranscranial magnetic stimulation (TMS) is a valuable technique for assessing the function of the motor cortex and cortico-muscular pathways. TMS activates the motoneurons in the cortex, which after transmission along cortico-muscular pathways can be measured as motor-evoked potentials (MEPs). The position and orientation of the TMS coil and the intensity used to deliver a TMS pulse are considered central TMS setup parameters influencing the presence/absence of MEPs. New methodWe sought to predict the presence of MEPs from TMS setup parameters using machine learning. We trained different machine learners using either within-subject or between-subject designs. ResultsWe obtained prediction accuracies of on average 77 % and 65 % with maxima up to up to 90 % and 72 % within and between subjects, respectively. Across the board, a bagging ensemble appeared to be the most suitable approach to predict the presence of MEPs. ConclusionsAlthough within a subject the prediction of MEPs via TMS setup parameter-based machine learning might be feasible, the limited accuracy between subjects suggests that the transfer of this approach to experimental or clinical research comes with significant challenges.

Effect of standardized ileal digestible arginine:lysine on growth performance of 6- to 13-kg nursery pigs.

A total of 480 newly weaned pigs (PIC 337×1050; Genus, Hendersonville, TN) with an initial body weight (BW) of 6.20 ± 0.61kg were used in a dose-response study to investigate the impact of increasing standardized ileal digestible (SID) Arg:Lys on nursery pig growth performance. At weaning, pigs were placed into 48 pens with 5 barrows and 5 gilts per pen. Pens were randomly assigned to 1 of 6 dietary treatments. The experimental diets were formulated with increasing SID Arg:Lys, achieved by substituting corn starch, glycine, and l-alanine with l-arginine, resulting in SID Arg:Lys ranging from 45% to 145%. Diets were sublimiting in SID Lys and exceeded all other essential amino acid requirements. The experimental diets were fed across two feeding phases from days 0 to 10 and 10 to 27, with adjustments made to account for the Lys requirement of the pigs. All pens were placed on a common diet for the remaining 14 d of the study to evaluate carryover effects. Pigs and feeders were weighed at the start and end of each phase to calculate average daily gain (ADG), average daily feed intake (ADFI), and feed efficiency (G:F). Data were analyzed according to a linear regression model, which included the linear and quadratic effects of SID Arg:Lys and initial BW. Pen was the experimental unit, and results were considered significant at P ≤ 0.05 and a tendency at 0.50 < P ≤ 0.10. From days 0 to 27, Arg:Lys tended to have a quadratic effect on ADFI (P = 0.058), where 97.00 ± 7.631% SID Arg:Lys maximized feed intake. Similarly, Arg:Lys had a quadratic impact on ADG (P = 0.046), where ADG was maximized at a SID Arg:Lys of 95.65 ± 7.165. Correspondingly, Arg:Lys had a quadratic effect on pig BW on day 27 (P = 0.014). These effects were carried through the end of the study, where Arg:Lys quadratically impacted days 0 to 41 ADFI (P = 0.006), ADG (P = 0.077), and day 41 BW (P = 0.028). There was no evidence of an effect of SID Arg:Lys on G:F throughout the study (P ≥ 0.315). In conclusion, SID Arg:Lys quadratically impacted ADFI and ADG in 6- to 13-kg nursery pigs, where ADFI was maximized at a SID Arg:Lys of 97.00% (95% CI [81.6%, 112.4%]), and ADG was maximized at a SID Arg:Lys of 95.65% (95% CI [81.2%, 110.1%]). Together, these data suggest that the SID Arg:Lys requirement of nursery pigs is at least 81%, based on the lower bounds of the 95% CI for maximum ADG and ADFI, and excessive Arg supplementation may negatively affect growth performance.

Assessing the influence of sleep and sampling time on metabolites in oral fluid: implications for metabolomics studies

IntroductionThe human salivary metabolome is a rich source of information for metabolomics studies. Among other influences, individual differences in sleep-wake history and time of day may affect the metabolome.ObjectivesWe aimed to characterize the influence of a single night of sleep deprivation compared to sufficient sleep on the metabolites present in oral fluid and to assess the implications of sampling time points for the design of metabolomics studies.MethodsOral fluid specimens of 13 healthy young males were obtained in Salivette® devices at regular intervals in both a control condition (repeated 8-hour sleep) and a sleep deprivation condition (total sleep deprivation of 8 h, recovery sleep of 8 h) and their metabolic contents compared in a semi-targeted metabolomics approach.ResultsAnalysis of variance results showed factor ‘time’ (i.e., sampling time point) representing the major influencer (median 9.24%, range 3.02–42.91%), surpassing the intervention of sleep deprivation (median 1.81%, range 0.19–12.46%). In addition, we found about 10% of all metabolic features to have significantly changed in at least one time point after a night of sleep deprivation when compared to 8 h of sleep.ConclusionThe majority of significant alterations in metabolites’ abundances were found when sampled in the morning hours, which can lead to subsequent misinterpretations of experimental effects in metabolomics studies. Beyond applying a within-subject design with identical sample collection times, we highly recommend monitoring participants’ sleep-wake schedules prior to and during experiments, even if the study focus is not sleep-related (e.g., via actigraphy).

Unraveling the dynamics of loneliness and cognition in late life: a cross-lagged panel model.

Loneliness and cognitive decline are pressing concerns among older adults, yet little research has explored cognition as a predictor of loneliness. This study investigates the dynamic relationship between loneliness and cognitive function in older adults using the random intercept cross-lagged panel model (RI-CLPM). Data were drawn from Waves 9-14 of the Health and Retirement Study (HRS), encompassing 8,473 individuals aged 65 years and older. Loneliness was assessed using the UCLA Loneliness Scale, and cognitive function was measured using immediate and delayed word recall and serial 7s from the HRS RAND file. Age, gender, education, marital status, self-health report, and depression were included as covariates. Using Mplus, we computed RI-CLPMs. The first three models were conducted on loneliness and cognitive functions. Then unconditional RI-CLPMs with no exogenous predictors were computed. Three conditional model results showed that age, gender, marital status, self-health report, and depression were significantly associated with loneliness in the first wave, but only age and self-health report were significantly associated with immediate and delayed word recall at the first wave, not with serial 7s. For carry-over effects, loneliness showed significant positive associations across consecutive waves, but cognitive functions showed significant positive associations just in the last two waves. Some spill-over effects were found between loneliness and cognitive functions. For within-person effects, although initially non-significant, a negative association between loneliness and immediate and delayed word recall emerged in later waves (11-12 and 13-14). The conditional models indicated that older age, not being married, male gender, low self-reported health, and high depression levels were positively associated with loneliness. However, only older age and lower self-reported health were positively linked to cognitive functions. This study underscores the link between loneliness and cognitive function decline in older adults, emphasizing the need to address loneliness to potentially reduce cognitive decline. Insights into demographic predictors of loneliness and cognitive function could inform targeted interventions for promoting successful aging.

Remote self-testing for adult cochlear implant users: the effect of wireless streaming on speech recognition in noise

Objectives Wireless sound transfer methods for cochlear implant sound processors have become popular for remote self-assessed hearing tests. The aim of this study was to determine (1) spectral differences in stimuli between different wireless sound transfer options and (2) the effect on outcomes of speech recognition tests in noise. Design In study 1, the frequency response of different streaming options (Phonak Roger Select, Cochlear Mini Mic 2+, telecoil and Bluetooth connection) was measured by connecting headphones to CI sound processors. Study 2 followed a repeated measures design in which digits-in-noise (DIN) tests were performed using wireless streaming to sound processors from Cochlear, Advanced Bionics, and MED-EL. Study Sample 20 normal hearing participants. Results Differences in frequency response between loudspeaker and wireless streaming conditions were minimal. We did not find significant difference in DIN outcome (F4,194 = 1.062, p = 0.376) between the wireless transfer options with the Cochlear Nucleus 7 processor. No significant difference in DIN outcomes was found between Bluetooth streaming and the loudspeaker condition for all of the three tested brands. The mean standard error of measurement was 0.72 dB. Conclusions No significant differences in DIN test outcomes between wireless sound transfer and the reference method were found.

Does Cannabis-based Medicine Improve Pain and Sleep Quality in Patients With Traumatic Brachial Plexus Injuries? A Triple-blind, Crossover, Randomized Controlled Trial.

After traumatic brachial plexus injuries, despite treatment with appropriate medications, some patients experience uncontrollable pain, which can be devastating. Cannabis-based medicine is considered to have pain-relieving benefits in this situation, but the evidence is limited. Is cannabis-based medicine effective compared with the placebo in (1) reducing pain (measured by the VAS for pain) and neuropathic pain (measured by the DN4 questionnaire), and (2) improving sleep quality (measured by the VAS for sleep quality) in patients with traumatic brachial plexus injury? This prospective, triple-blinded (the researcher administering the substance, the patients, and the evaluator were all blinded to the substance used), two-period crossover, placebo-controlled, randomized controlled trial was conducted at a single center. Between January 2020 and January 2022, we treated 147 patients for neuropathic pain related to a traumatic brachial plexus injury. Our inclusion criteria were age between 20 and 60 years and moderate-to-severe pain (VAS for pain equal to or greater than 4 of 10 for more than 6 months), even with the use of appropriate medications. Based on these criteria, 20% (30) of patients were eligible. They were randomly allocated to receive either cannabis-based medicine followed by the placebo or vice versa. Fifteen patients received cannabis-based medicine first, and 15 patients received the placebo first. The groups did not differ at baseline in terms of demographic parameters. Participants received both the cannabis-based medicine and the placebo; they started with 10 days of the initial intervention, followed by a 14-day washout period, and then a 10-day period with the second intervention. The dosage regimen adhered strictly to the protocol. The outcomes were the (1) VAS for pain, which ranges from 0 to 10 and where 0 represents no pain and 10 signifies the worst pain; (2) the DN4 questionnaire which ranges from 1 to 10 and where a score of 4 or higher indicates a positive result for neuropathic pain; and (3) VAS for sleep quality, from 0 (worst) to 10 (best). The minimum clinically important difference of VAS for pain was defined as a 2-point improvement. After enrollment, 7% (2 of 30) of patients (one patient received the cannabis-based medicine first and another received the placebo first) were lost before the minimum study follow-up, leaving 93% (28 of 30) for analysis. With 28 patients in each group, the study was powered a priori at 90% to detect a clinically important difference of 2 points in the VAS for pain. No carryover or period effects were observed in the study. Four patients experienced mild dizziness during the cannabis-based medicine period but were able to continue the intervention. When comparing the use of cannabis-based medicine alongside pain control medications with the combination of placebo and pain control medications, the reduction in pain VAS from the preintervention resulted in a mean difference of 1 (99% CI -0.03 to 2.1; p = 0.01). Neuropathic pain was reported by 75% (21 of 28) of patients in both interventions (OR 1 [99% CI 0.07 to 14.1]; p > 0.99). The VAS for sleep quality favored cannabis-based medicine with a mean difference of 1.5 (99% CI 0.7 to 2.4; p < 0.001). Our study findings indicate that cannabis-based medicine did not improve pain by a clinically important margin. Consequently, our study advises against the addition of cannabis-based medicine to the standard medication treatment for pain in patients with traumatic brachial plexus injury. Level 1, therapeutic study.

Impact of Hearing Aid Processing Delay on Stop Consonant Voicing Perception in Open Fittings.

Hearing aid (HA) processing delay results in asynchronous overlap of the input sound and the delayed amplified sound at the eardrum in open-ear fittings. This may distort the temporal cues used for stop-consonant voicing distinctions. The current study evaluated the impact of HA processing delay on voiced-voiceless categorization of syllable initial consonants /d/ and /t/ for a range of voice onset times (VOTs). Nineteen older listeners (Mage = 73 years) with mild-moderate sensorineural hearing loss participated. All listeners performed the voiced-voiceless categorization task in double-blind within-subjects design. Thirteen stimulus tokens along the /di:/ - /ti:/ continuum were created by varying VOTs. Stimuli were then processed using an HA simulator, which simulated the overall sound pressure levels measured at the eardrum in open-ear fittings with four processing delay times (0, 0.5, 5, and 8 ms). Individualized stimuli were generated for each listener based on their audiogram and presented via calibrated ear inserts at the most comfortable listening level. Performance across all VOT intervals was fitted with psychometric functions, which were then used to estimate the voiced-voiceless crossover point and the slope parameter for each simulated delay condition. The crossover point along the voiced-voiceless continuum shifted systematically with increased processing delay toward voiced /di:/ over unvoiced /ti:/ percepts. The shift in the crossover point between the 0-ms reference condition and the 8-ms processing delay condition corresponded to 5.8 ms of change in VOT. The 8-ms processing delay condition resulted in significantly shallower categorization slopes compared to the 0- and 0.5-ms delay conditions. Temporal distortions created by HA processing delay in open-ear fittings impacted voicing perception of syllable initial stop-consonant stimuli near the voiced-voiceless crossover point. Short HA processing delay should be considered for open-ear fittings to preserve the natural VOT cues used for voicing detection.

A Life Cycle and Economic Assessment of the Peggy Guggenheim Collection in Venice for Environmental and Economic Sustainability

This paper applies selected methodologies for the measurement of the environmental and economic sustainability of the Peggy Guggenheim Collection (PGC) in Venice with a view to assessing the PGC’s sustainability and commitment to implementing selected SDGs. To assess environmental sustainability, a life cycle assessment (LCA) has been carried out. The museum is conceptualized as a “firm” that produces several outputs and needs several inputs. The results provide the number of annual CO2e (and other pollutants) emissions linked to the regular activity of the museum. The environmental cost (in EUR), linked to the impacts obtained from LCA, has been calculated. To assess economic sustainability, a survey and econometric methods were used to value services directly generated by the museum, and input/output methods were used to compute the direct and indirect impacts on the local economy. Nonetheless, PGC visitors (those who travel to Venice with the main objective of visiting the PGC) contribute to around 1.2%/1.4% of Venice’s GDP. The results from input–output tables show that, although the final demand generated by the PGC’s own activities amounted to about EUR 620 million in 2022, the economic benefits of the PGC beyond this final demand are significant and very positive due to carry-over effects. Specifically, the PGC leads to an increase in GDP of around EUR 1.200 million, with a multiplier of 1.9. In terms of employment, around 8200 jobs are associated with the presence of the PGC. The net public finance revenue also clearly benefits, with a net income of around EUR 150 million in 2022. Comparing both the environmental and economic impacts of the PGC, one can conclude that the annual activities performed by the museum are highly sustainable, with the economic pillar strongly offsetting the costs generated using natural resources. The creation of economic value, therefore, is generated in respect of environmental boundaries, even if some minor flaws can be highlighted. The connection between museums and sustainable development goals is highly recognized. The findings show the PGC’s commitment to achieving and implementing selected SDGs, including SDG 4, SDG 11, and SDG 16, by implementing actions and strategies that are aligned with these goals.

Determination of ivermectin in plasma and whole blood using LC-MS/MS.

Ivermectin is a widely used drug for the treatment of helminthiasis and filariasis worldwide, and it has also shown promise for malaria elimination through its potent mosquito-lethal activity. The objective of this study was to develop and validate a high-throughput and sensitive method to quantify ivermectin in plasma and whole blood samples, using automated sample extraction followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Phospholipids were removed in patient whole blood (100 µl) and plasma (100 µl) samples using a 96-well plate Hybrid-solid phase extraction technique. Ivermectin and its isotope-labelled internal standard (ivermectin-D2) were separated on an Agilent Poroshell 120 EC-C18 50mm × 3.0mm I.D. 2.7µm, using a mobile phase of acetonitrile: ammonium formate 2 mM containing 0.5% formic acid (90: 10, v/v). Detection was performed using a triple quadrupole mass spectrometer in the positive ionization mode. The method was validated in the concentration range 0.970 - 384 ng/ml in both plasma and whole blood matrices. Intra- and inter-batch precisions during the validation were below 15%. There was no carryover or matrix effects detected. Ivermectin is a stable compound and results showed no degradation in the different stability tests. The validated method proved to have high sensitivity and precision, good selectivity and to be suitable for clinical application or laboratory quantification of ivermectin in plasma or whole blood samples.

The Impact of Lunch Timing on Nap Quality.

Previous research has established that food intake is a biological regulator of the human sleep-wake cycle. As such, the timing of eating relative to sleep may influence the quality of sleep, including daytime naps. Here, we examine whether the timing of lunch (1 h vs. 2 h interval between lunch and a napping opportunity) impacts the quality of an afternoon nap. Using a randomized within-subject design over two separate experimental sessions (7 days apart), participants (n = 40, mean age = 25.8 years) consumed lunch 1 h and 2 h prior to an afternoon nap opportunity. Polysomnography and subjective self-reports were used to assess sleep architecture, sleepiness levels, and nap quality. Results revealed no significant differences in subjective ratings of sleep quality and sleepiness, or in sleep architecture (total sleep time, sleep efficiency, sleep onset latency, sleep stages) between the 1 h and 2-h lunch conditions. All sleep measures were similar when napping followed eating by either 1 h or 2 h, suggesting that eating closer to nap onset may not negatively impact sleep architecture and quality. Future research should continue to identify conditions that improve nap quality, given the well-documented benefits of naps to reduce sleep pressure and improve human performance.

A Pilot Study Investigating the Feasibility, Reach-Out, Acceptability, Fidelity, and Efficacy of a Group Laughie Prescription on the Well-Being of Earthquake Survivors in Türkiye

Mental health and quality of life may be negatively impacted among earthquake survivors. This pilot study evaluates the use of and reports on the preliminary effects of participating in a 1-minute Laughie (Laugh Intentionally Everyday) Laughter Prescription on well-being in earthquake survivors in Türkiye. A Group Laughie intervention was delivered using a within-subject (n = 20; M age = 34.78 ± 6.65; 14 female) pretest-posttest design. Group Laughies were delivered once a day over 2 weeks with participants prescribed to further laugh with the Group Laughie recording twice a day, resulting in 3 minutes of intentional laughter daily. Data were collected using a range of questionnaires to track feasibility, reach-out, acceptability, fidelity, and efficacy, including Laughie Checklists, a Post-Intervention Perceived Impact Measure (PIPIM) in the form of the Positive Psychology One-off Post-intervention measure (PPOP), and the World Health Organization (WHO-5) well-being index. Results indicated high intervention fidelity using Laughie Checklists and positive post-intervention perceived impact using the 11-point Likert-scaled PPOP (x = 7.62 ± 1.44). After the intervention, a statistically significant difference (p < 0.001) was found between WHO-5 well-being index pretest scores (x = 2.16 ± 1.00) and post-test (x = 4.08 ± 0.24). This study demonstrated beneficial effects of the Laughie prescription on earthquake survivors. This is the first intervention to explore an online Group Laughie intervention. Health professionals and especially nurses can use the 1-minute Laughie prescription in the form of a Group Laughie intervention to increase the well-being of individuals and improve perceived mental health.

Efficacy, crop response, and economics of alternatives to glyphosate for pre‐plant weed control in small grains

AbstractThe value of glyphosate to growers practicing minimum or no‐till farming has been primarily a function of three factors: broad‐spectrum weed control, little to no carryover effect, and cost effectiveness. A 2‐year field study was conducted in 2021 and 2022 at the University of Idaho Research and Extension Centers at Kimberly and Aberdeen, ID, to evaluate the efficacy of alternative pre‐plant burndown herbicide treatments as compared to glyphosate treatment, the industry standard in small grain production systems. Herbicide cost comparison and crop injury observation were also conducted. Most herbicide treatments provided 90% control of the predominant weed species at 3 weeks after herbicide application. At least six different herbicide treatments had an equivalent cost to that of glyphosate at $26.50 ha−1. No observable damage from herbicide treatments was observed and crop yield was not affected by the treatments. These factors indicate that there are alternatives to pre‐plant burndown herbicides that are equally effective as glyphosate. With combinations of herbicides, each having a different site of action, weed control can be achieved while simultaneously reducing the risk of herbicide resistance.

Effects of labyrinth-like path designs on mitigating stress response to traffic noise

Traffic noise poses significant mental health risks primarily through stress responses. Although labyrinths have been proven to reduce stress and promote relaxation, their efficacy in mitigating stress induced by traffic noise remains unclear. To address this issue, this study conducted repeated-measures experiments to investigate the effects of different labyrinth-like path designs on stress responses to traffic noise. Heart rate variability and electrodermal activity were measured using the E4 wristband to evaluate participants’ physiological stress levels. The results suggest that: (1) Walking on a continuous curved path layout (CS) effectively mitigated stress responses to traffic noise, as indicated by multiple physiological stress measures; (2) Regarding stress-mitigating path continuity attributes, 180-degree turns were positive attributes (p < 0.05), while 90-degree turns were negative attributes (p < 0.01); (3) Regarding stress-mitigating path layout features, curved segments (p < 0.01) and switchbacks (p < 0.05) were positive features, while U shapes were negative features (p < 0.01). This study indicates that labyrinth-like path designs can effectively mitigate stress responses to traffic noise; however, complex path layouts with frequent directional changes should be avoided in noisy environments, particularly in areas lacking resources for sound barriers and noise-absorbing vegetation. Our findings contribute to the development of evidence-based guidelines for designing stress-mitigating streets in high-traffic environments. When mainstreaming these guidelines through public codes, further comprehensive analyses including feasibility assessments, economic evaluations, and community engagement are warranted to ensure practical and sustainable implementation.

Lysine from Whole Wheat Bread Consumed by Healthy Adult Males Has High Metabolic Availability When Assessed Using the Indicator Amino Acid Oxidation Method

BackgroundThe protein quality of wheat is limited by its low content of the indispensable amino acid (AA) lysine and the metabolic availability (MA) of lysine in wheat bread for humans is unknown. ObjectivesThe study objective was to determine the MA of lysine in whole wheat bread. MethodsFive healthy young males (≤30 y, body mass index <25) were studied in a repeated-measures design using the indicator amino acid oxidation (IAAO) method, with L-[1-13C] phenylalanine as the indicator. Each received 7 levels of lysine intakes in random order; 4 levels of L-lysine; 5, 8, 12, and 15 mg/kg/d (reference diet), and 3 intakes of lysine from whole wheat bread (test diet). The MA of lysine in whole wheat bread was assessed by comparing the IAAO response to the test diet (whole wheat bread) with the IAAO response to the reference diet using the slope ratio method. ResultsThe MA of lysine from whole wheat bread was 90%. ConclusionsLysine has a high MA but it is still limiting in whole wheat bread due to its low concentration. A combination of wheat with a complementary protein source (that is, lentils which are sufficient in lysine) is recommended to meet the lysine requirement in a wheat-based diet for healthy young males.This trial was registered at clinicaltrials.gov as NCT03674736 and NCT03200652.

Lunar rhythms and their carry-over effects may shape environmental sex determination in a coral reef fish.

Lunar rhythms shape spawning phenology and subsequent risks and rewards for early life-history stages in the sea. Here, we consider a perplexing spawning phenology of the sixbar wrasse (Thalassoma hardwicke), in which parents spawn disproportionately around the new moon, despite the low survival of these larvae. Because primary sex determination in this system is highly plastic and sensitive to social environments experienced early in development, we ask whether this puzzling pattern of spawning is explained by fitness trade-offs associated with primary sexual maturation. We used otoliths from 871 fish to explore how spawning on different phases of the moon shapes the environments and phenotypes of settling larvae. Offspring that were born at the new moon were more likely to settle (i) before other larvae, (ii) at a larger body size, (iii) at an older age, (iv) to the best quality sites, and (v) as part of a social group-all increasing the likelihood of primary maturation to male. Selection of birthdates across life stage transitions suggests that the perplexing spawning phenology of adults may reflect an evolutionarily stable strategy that includes new moon spawning for compensatory benefits later in life, including preferential production of primary males at certain times.

The coach bots are coming: exploring global coaches’ attitudes and responses to the threat of AI coaching

ABSTRACT Artificial intelligence (AI) in business coaching, as in other human resource development professional service roles, opens up the possibility of multiple chances, such as cost- and time-effective gains. However, as AI can act autonomously and may surpass human performance, it can both lead to unforeseen risks as well as create a threat for professional service workers, including business coaches. Using a within-subject threat manipulation design, the present research investigated whether business coaches (N = 436; from over 50 different countries) respond to the topic of AI in coaching with heightened threat-related affective states and how this change affects their attitude towards the topic. Expectedly, the topic evoked higher behavioral inhibition and lower behavioral activation threat-related affective states, leading to lower curiosity in and a more negative opinion of AI in coaching. Theoretical and practical implications are addressed to lower the coaches’ threat-related affective states towards AI application in professional services. A hybrid approach between responsible AI and an ethically skilled and professionally trained coach is recommended.

Somatic nuclear mitochondrial DNA insertions are prevalent in the human brain and accumulate over time in fibroblasts.

The transfer of mitochondrial DNA into the nuclear genomes of eukaryotes (Numts) has been linked to lifespan in nonhuman species and recently demonstrated to occur in rare instances from one human generation to the next. Here, we investigated numtogenesis dynamics in humans in 2 ways. First, we quantified Numts in 1,187 postmortem brain and blood samples from different individuals. Compared to circulating immune cells (n = 389), postmitotic brain tissue (n = 798) contained more Numts, consistent with their potential somatic accumulation. Within brain samples, we observed a 5.5-fold enrichment of somatic Numt insertions in the dorsolateral prefrontal cortex (DLPFC) compared to cerebellum samples, suggesting that brain Numts arose spontaneously during development or across the lifespan. Moreover, an increase in the number of brain Numts was linked to earlier mortality. The brains of individuals with no cognitive impairment (NCI) who died at younger ages carried approximately 2 more Numts per decade of life lost than those who lived longer. Second, we tested the dynamic transfer of Numts using a repeated-measures whole-genome sequencing design in a human fibroblast model that recapitulates several molecular hallmarks of aging. These longitudinal experiments revealed a gradual accumulation of 1 Numt every ~13 days. Numtogenesis was independent of large-scale genomic instability and unlikely driven by cell clonality. Targeted pharmacological perturbations including chronic glucocorticoid signaling or impairing mitochondrial oxidative phosphorylation (OxPhos) only modestly increased the rate of numtogenesis, whereas patient-derived SURF1-mutant cells exhibiting mtDNA instability accumulated Numts 4.7-fold faster than healthy donors. Combined, our data document spontaneous numtogenesis in human cells and demonstrate an association between brain cortical somatic Numts and human lifespan. These findings open the possibility that mito-nuclear horizontal gene transfer among human postmitotic tissues produces functionally relevant human Numts over timescales shorter than previously assumed.

Isokinetic Peak Torque Improvement and Shoulder Muscle Ratios Imbalance Correction After Specific Strength Training on a New Ballistic Throwing Device: A Randomized Controlled Trial.

The aim of this study was to investigate the effects of 8-week ballistic-strength-training program using a validated specific throwing device (ie,Arm/Shoulder Specific Strength Device), on isokinetic shoulders' rotation muscle-torques and ratios as well as range of motion in team handball players. A repeated-measures experimental design with a randomized controlled trial was used. Twenty-six high-level competitive male U-19 team handball players were randomly assigned into training (TG, n = 15) and control (n = 11) groups. The TG undertook a twice a week for 8-week periodized throws program with an individually predetermined optimal load. The program incorporated shackled eccentric and concentric exercises using the Arm/Shoulder Specific Strength Device. Peak torques, functional, and conventional ratios for both arms at different angular velocities (60°·s-1, 180°·s-1, and 300°·s-1) were assessed over time and between groups, using an isokinetic dynamometer. A significant improvement for TG (P < .01; d = 1.13 [moderate]; +20.2%) of the concentric peak torques for dominant arm in external rotation was observed at 300°·s-1. Significant (P < .05-.01) increases were also noted for nondominant arm at the 3 studied angular velocities. In addition, 300°·s-1 eccentric peak torques of the dominant arm and nondominant arm have significantly improved for both external and internal rotations (P < .05; d = .99 [moderate] and d = 1.21 [large]; +15.7% and + 17.9%, respectively) with small changes at the other angular assessed velocities. Posttraining, TG's dominant arm showed significant improvements (P < .05-.01) in functional and conventional ratios at all velocities. Notably, significant differences (P < .05-.01) were observed at 60°·s-1 and across all velocities when comparing the TG with the control group. TG showed significant increase for internal rotation and external rotation shoulder range of motions (P < .05; d = 1.22-1.27 [large]), +5.0% and +7.7%, respectively). The specific 8-week throwing training program on the Arm/Shoulder Specific Strength Device showed significant performance improvements in almost all assessed isokinetic concentric and eccentric peak torques as well as internal and external range of motion increase for both arms while ensuring rotator cuff torque ratios and shoulder mobility in team handball real sport-specific condition.