Total Phosphorus Removal Efficiencies Research Articles

Stimulating carbon and nitrogen metabolism of Chlorella pyrenoidosa to treat aquaculture wastewater and produce high-quality protein in plate photobioreactors

Wastewater treatment by microalgae is the economical and environmentally friendly strategy, but is still challenged with the strict discharge standards and valuable biomass exploitations. The carbon and nitrogen metabolism of Chlorella pyrenoidosa was improved by the red LED light and starch addition to treat Tilapia aquaculture wastewater (T-AW) and produce protein simultaneously in a plate photobioreactor. The red LED light was applied to improve the nutrient removals at an outdoor temperature, but the concentrations except total nitrogen did not satisfy the discharge standards. After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L. At a molecular level, the metabolic flux and transcriptome analyses showed that red light promoted carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle, and upregulated the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis. The harvesting C. pyrenoidosa possessed the 62 % essential amino acids and great lipid composition for biofuels. This study provided a new orientation for outdoor wastewater treatment and protein production by collaboratively regulating the carbon and nitrogen metabolism of microalgae.

Integration of microalgae cultivation and anaerobic co-digestion with dairy wastewater to enhance bioenergy and biochemicals production

A sequential anaerobic digestion and phycoremediation process was employed to recover nutrients and remove pollutants from dairy wastewater (DW), while simultaneously producing biomethane and biochemicals. Anaerobic digestion of 100% DW achieved a methane content and production rate of 53.7% and 0.17 L/L/d, respectively. This was accompanied by the removal of 65.5% chemical oxygen demand (COD), 86% total solid (TS), and 92.8% volatile fatty acids (VFAs). The anaerobic digestate was then used to grow Chlorella sorokiniana SU-1. Using 25% diluted digestate as the medium, SU-1 could reach 4.64 g/L biomass concentration, with total nitrogen (TN), total phosphorus (TP) and COD removal efficiencies of 77.6%, 87.1% and 70.4%, respectively. The obtained microalgal biomass (contained 38.5% carbohydrates, 24.9% proteins, 8.8% lipids) was used to co-digest with DW, resulting in good methane production performance. Co-digestion with 25% (w/v) algal biomass obtained a higher CH4 content (65.2%) and production rate (0.16 L/L/d) than other ratios.

Effects of carbon sources on phosphorus speciation transformation process, characteristics of extracellular polymeric substances, and microecology in anaerobic systems

The effects of different carbon sources on the transformation process of the form of phosphorus and microecology were examined in an anaerobic system using glucose and L-malic acid as carbon sources. Results showed that for these two carbon sources (glucose and L-malic acid), the average removal efficiencies of chemical oxygen demand by up-flow anaerobic sludge bed reactors were 97.16 % and 88.63 %, and the average removal efficiencies of total phosphorus were 11.38 % and 5.68 %, respectively. The average concentrations of phosphine in anaerobic reactors using glucose and L-malic acid as carbon sources were 160.73 mg/m3 and 90.80 mg/m3 over days 24–40, respectively. When glucose was used as carbon source, the proportions of biologically available phosphorus (i.e., the sum of non-apatite inorganic phosphorus and organic phosphorus content) within total phosphorus in sludge on days 0, 20, and 40 were 76.22 %, 77.90 %, and 85.04 %, respectively. Glucose could better promote the enhancement of dehydrogenase activity than L-malic acid. The carbon source affected the activity of alkaline phosphatase, and the enzyme activity was stronger when glucose was used as carbon source. Kyoto Encyclopedia of Genes and Genomes functional analysis showed that amino acid metabolism and carbohydrate metabolism dominated. On days 0, 30 and 60, the relative abundances of carbohydrate metabolism in microorganisms using glucose as carbon source were 0.26 %, 0.36 %, and 0.34 % higher than those with L-malic acid as carbon source, respectively.

Evaluation of biogas production and pollutant removal efficiency of two-phase anaerobic digestion treating slaughterhouse effluent

Treatment of slaughterhouse and other agro-processing industry effluent has become important due to continuous global population growth and meat demand, particularly in developing countries, for sustainable biowaste management as well as value additions such as biofuel. Therefore, the objective of the study was to evaluate the performance of two-phase anaerobic digestion of slaughterhouse wastewater in terms of biogas production, methane yield, and removal efficiencies of organic matter, total nitrogen, and total phosphorus. Two consecutively connected 40 L galvanized metal anaerobic batch bioreactors were used to conduct the experiment. The total phosphorus removal efficiencies of hydrolytic-acidogenic reactor and methanogenic reactor were 13.34% and 16.58%, respectively. Total chemical oxygen demand, soluble chemical oxygen demand, biological oxygen demand, total dissolved solids, total solids, total suspended solids, volatile solids, and turbidity had overall removal efficiencies of 82.87, 88.53, 93.32, 75.35, 95.55, 98.95, 97.42 and 98.06%, respectively. Biogas production of 189.45 mL/day with methane and carbon dioxide compositions of 67.69% and 29.9%, respectively, was also achieved. It was concluded that the two-phase anaerobic digestion of slaughterhouse wastewater shows substantial organic matter removal efficiencies and biogas production.

Insights on nitrogen and phosphorus removal mechanism in a single-stage Membrane Aeration Biofilm Reactor (MABR) dominated by denitrifying phosphorus removal coupled with anaerobic/aerobic denitrification

In the study, the denitrifying phosphorus removal process coupled with anaerobic/aerobic denitrification was started in a single-stage Membrane Aeration Biofilm Reactor (MABR). When the influent NO3−-N was 50.2 mg/L and the total phosphorus (TP) was 30.5 mg/L, the maximum Total Inorganic Nitrogen Removal Efficiency (TINRE) can reach 99.79 % and the Total Phosphorus Removal Efficiency (TPRE) can reach 82.62 % without the addition of any flocculant or precipitant. The phosphorus content in the substrate sludge was 4.10 %–6.15 %, which was higher than that in the initial biofilm or activated sludge (1.27 %–1.83 %). Based on 16S rRNA sequencing technology, it was found that Phosphorus-Accumulating Organisms (PAOs, Acinetobacter, relative abundance 0.02 % to 1.92 %) and Denitrifying Phosphorus-Accumulating Organisms (DPAOs, Flavobacterium (0.26 % to 5.39 %), Bdellovibrio (0.12 % to 1.48 %), and Pseudomonas (2.29 % to 1.28 %)) played pivotal roles in phosphorus removal. The enzyme gene reads of Complex I and Complex V processes were much more than that of Complex II- Complex IV, which indicated that Complex I and Complex V can be the main process for oxidative phosphorylation in the MABR. Moreover, four inorganic nitrogen metabolic processes, Dissimilatory Nitrate Reduction to Ammonium (DNRA), Assimilatory Nitrate Reduction Ammonium (ANRA), nitrification, and denitrification were found. Nitrifiers (Nitrosomonas (0.09 % to 0.19 %), Nitrospira (2.48 % to 0.06 %)), denitrifiers (Dokdonella (6.95 % to 2.11 %), Denitratisoma (0.42 % to1.48 %)), and aerobic denitrifiers (Pseudomonas (2.29 % to 1.28 %) and Thauera (4.75 % to 1.78 %)) contributed to nitrogen removal in the MABR. This study provided insights into the removal mechanism of nitrogen and phosphorus in a single-stage MABR.

Effects of powdered activated carbon dosage on the performance of membrane bioreactors treating biochemical tail water

As a promising treatment technology for wastewater, the promotion of membrane bioreactors (MBR) is restricted by biological fouling. Among the measures used to mitigate membrane fouling, the addition of powdered activated carbon (PAC) to MBRs has been recognized as an effective practice. However, the effects of PAC dosage on the performance of MBRs that treat highly biochemical influent from wastewater treatment plants remain unclear. In this study, by investigating the treatment of biochemical tailwater by PAC–MBRs, we thoroughly analyzed the effects of PAC dosage on the contamination removal efficiency, membrane operation cycles, sludge mixture properties, and microorganism distributions. The results indicated that the addition of PAC enhanced the removal efficiency of MBRs depending on the contaminant of interest. For example, the removal efficiency of total nitrogen can be boosted from 30 % to 60 % with PAC addition, while the removal efficiencies of total phosphorus were comparable with or without PAC addition. Furthermore, the application of PAC in MBRs can prolong the duration of membranes by suppressing biological fouling. This was supported by the decreased microbial products, reduced smaller solid particles, and stronger stability of sludge particles. PAC addition also boosts the proportion of Proteus and decreases the proportion of Bacteroides, which helps to improve the removal efficiencies of contaminants. Finally, among the PAC dosages tested in our study, 1.5 g/L PAC was proposed as the optimal candidate for treating highly biochemical influents. For example, the corresponding time for transmembrane pressure to reach 0.03 MPa was 19 d at 1.5 g/L PAC, while these periods were 7 and 14 d at dosages of 0 and 0.5 g/L, respectively. Overall, the findings of this study will aid in the selection of optimal dosages for other systems with different types of influents.

Enhanced efficiency and mechanism of low-temperature biochar on simultaneous removal of nitrogen and phosphorus by combined heterotrophic nitrification-aerobic denitrification bacteria

In this study, three strains of heterotrophic nitrification-aerobic denitrification (HN-AD) capable of simultaneously removing phosphorus were isolated from activated sludge, and low-temperature coconut shell biochar was prepared. The metabolic effects of combined HN-AD bacteria on the total nitrogen (TN) and total phosphorus (TP) were investigated, and the enhanced efficiency and mechanism of low-temperature biochar on the combined bacteria were also explored. The results indicated that the combined bacteria could adapt to environmental impacts and multiple nitrogen sources. The low-temperature biochar containing more aliphatic carbon and oxygen-containing functional groups enhanced the metabolic activity of combined HN-AD bacteria and accelerated the electron transfer process during nitrogen and phosphorus degradation. The removal efficiencies of TN and TP increased by 68% and 88%, respectively, in the treatment of actual sewage by biochar attached with combined bacteria. The findings form a basis for the engineering utilization of HN-AD and are of great practical significance.

The Pretreatment of Micro-Polluted Source Water through Phototrophic Biofilms under Variant Light Conditions

The imbalance of inorganic nutrients in micro-polluted source water poses a huge threat to aquatic environments and human health. To pretreat micro-polluted source water, some biological reactors have been conducted at lab-scale. However, using phototrophic biofilms to pretreat micro-polluted source water at pilot-scale has yet to be explored, and the effects of light on the practical operation of phototrophic biofilms are poorly understood. In this study, the potential of pretreating micro-polluted source water by phototrophic biofilms was explored. The high light intensity (4500 lx, 60.75 μmol/m2/s) promoted the growth of phototrophic biofilms and the secretion of extracellular polymeric substance. The removal efficiency of inorganic nitrogen and total phosphorus in synthetic micro-polluted water was 56.82% and 40.90%, respectively. When interacting with actual micro-polluted source water, the nutrients in the actual micro-polluted source water were reduced by the stable pilot-scale phototrophic biofilms. The final concentration of effluent nutrients was lower than the Grade II surface water quality standard in China. Therefore, using phototrophic biofilms as a pretreatment facility in water treatment plants is a promising solution to this issue.

Performance of different bacteria-microalgae-fungi consortium cultivation in nutrient removal and biogas upgrading by induction of GR24 and 5-Deoxystrigol

This study evaluated the effects of 5-Deoxystrigol (5 DS) concentration on the growth characteristics, the photosynthetic performance of different bacterial-microalgae-fungal symbiotic systems, and the simultaneous treatment of biogas slurry and biogas. Suitable 5 DS concentrations enhanced the metabolism and photosynthesis of microalgae in the symbiotic system, leading to the rapid growth and good purification performance of the symbionts. The growth and purification performance of symbiotic systems were affected more by fungal than algae species. Specifically, Ganoderma lucidum (G. lucidum) performed better than Pleurotus ostreatus (P. ostreatus), while Chlorella vulgaris (C. vulgaris) performed better than Scenedesmus obliquus (S. obliquus). The Bacillus licheniformis (B. licheniformis)-C. vulgaris-G. lucidum symbiote exhibited the best purification effectiveness for biogas slurry and biogas, with a removal efficiency of chemical oxygen demand, total nitrogen, total phosphorus, and CO2 of 84.71 ± 8.23%, 83.94 ± 8.21%, 86.48 ± 8.36%, and 68.74 ± 6.36%, respectively, under the recommended 10−11 M 5 DS. Moreover, the purification effectiveness was positively correlated with the growth characteristics and photosynthesis performance of the bacterial-microalgae-fungal co-culture system. The findings of this study will facilitate the wide application and development of symbiotic systems in biogas slurry treatment and biogas upgrading.

Effect of thermal hydrolyzed sludge filtrate as an external carbon source on biological nutrient removal performance of A2/O system

Thermal hydrolyzed sludge filtrate (THSF) rich in biodegradable organics could be a promising external carbon source for biological nutrient removal (BNR). The use of THSF can effectively reduce wastewater treatment plants operating costs and recover bioresources and bioenergy from the waste activated sludge. In this study, the effect of THSF on the BNR process was investigated using a lab-scale anaerobic/anoxic/oxic (A2/O) system. Total nitrogen (TN) and total phosphorus (TP) removal efficiencies of 74.26 ± 3.36% and 92.20 ± 3.13% at a 0.3% dosing ratio were achieved, respectively. Moreover, 20.42% of the chemical oxygen demand (COD) contained in THSF contributed to denitrification, enhancing nitrogen removal efficiency from 55.30 to 74.26%. However, the effluent COD increased by approximately 36.80%, due to 18.39% of the COD contained in THSF discharged with effluent. In addition, the maximum denitrification rate was approximately 16.01 mg N g VSS-1 h-1, while the nitrification rate was not significantly affected by THSF. Nitrosomonas, a common chemoautotrophic nitrifier, was not detected after the introduction of THSF. The aerobic denitrifier Rubellimicrobium was stimulated, and its relative abundance increased from 0.16 to 3.03%. Moreover, the relative abundance of Dechloromonas was 3.93%, indicating that the denitrifying phosphorus removal process was enhanced. This study proposes an engineering application route of THSF, and the chemical phosphate removal pretreatment might be a means to suppress the phosphate recirculation.

Chitosan-coated oleaginous microalgae-fungal pellets for improved bioremediation of non-sterile secondary effluent and application in carbon dioxide sequestration in bubble column photobioreactors

Oleaginous microalga Scenedesmus sp. SPP was rapidly immobilized in oleaginous fungal pellets by their opposite-surface-charges. Microalgae-fungal (MF) pellets were more effective in bioremediation of non-sterile secondary effluent than mono-culture. The optimal hydraulic retention time for dual bioremediation in semi-continuous mode was 72 h. The MF pellets coated with 0.4 %-chitosan improved removal efficiencies of COD, total nitrogen (TN), and total phosphorus (TP) up to 96.2±0.0 %, 88.2±2.8 % and 71.5±0.7 %, respectively, likely because of better cell retention and more nutrient adsorption and assimilation. Dual bioremediation by coated MF pellets was also successfully scaled up in 30-L bubble-column photobioreactors with improved COD, TN, and TP removal efficiencies of 98.5±0.0 %, 90.2±0.0 % and 79.5±2.1 %, respectively. This system also effectively removed CO2 from simulated flue gas at 71.2±0.4 % and produced biomass with high lipid content. These results highlight the effectiveness of bio-immobilization by fungal pellets; chitosan coating; and their practical applications in bioremediation and CO2 sequestration.

Removal efficiency prediction model based on the artificial neural network for pollution prevention in wastewater treatment plants

PurposeArtificial intelligence (AI) models are demonstrating day by day that they can find long-term solutions to improve wastewater treatment efficiency. Artificial neural networks (ANNs) are one of the most important of these models, and they are increasingly being used to forecast water resource variables. The goal of this study was to create an ANN model to estimate the removal efficiency of biological oxygen demand (BOD), total nitrogen (TN), total phosphorus (TP) and total suspended solids (TSS) at the effluent of various primary and secondary treatment methods in a wastewater treatment plant (WWTP).Design/methodology/approachThe MATLAB App Designer model was used to generate the data set. Various combinations of wastewater quality data, such as temperature(T), TN, TP and hydraulic retention time (HRT) are used as inputs into the ANN to assess the degree of effect of each of these variables on BOD, TN, TP and TSS removal efficiency. Two of the models reflect two different types of primary treatment, while the other nine models represent different types of subsequent treatment. The ANN model’s findings are compared to the MATLAB App Designer model. For evaluating model performance, mean square error (MSE) and coefficient of determination statistics (R2) are utilized as comparative metrics.FindingsFor both training and testing, the R values for the ANN models were greater than 0.99. Based on the comparisons, it was discovered that the ANN model can be used to estimate the removal efficiency of BOD, TN, TP and TSS in WWTP and that the ANN model produces very similar and satisfying results to the APPDESIGNER model. The R-value (Correlation coefficient) of 0.9909 and the MSE of 5.962 indicate that the model is accurate. Because of the many benefits of the ANN models used in this study, it has a lot of potential as a general modeling tool for a range of other complicated process systems that are difficult to solve using conventional modeling techniques.Originality/valueThe objective of this study was to develop an ANN model that could be used to estimate the removal efficiency of pollutants such as BOD, TN, TP and TSS at the effluent of various primary and secondary treatment methods in a WWTP. In the future, the ANN could be used to design a new WWTP and forecast the removal efficiency of pollutants.

Simultaneous treatment of phosphorus and fluoride wastewater using acid-modified iron-loaded electrode capacitive deionization: Preparation and performance

Here, capacitive deionization technology (CDI) using modified activated carbon fiber felt (ACF) electrodes was proposed to provide a new strategy for the challenge of simultaneous phosphorus and fluoride wastewater treatment. The acid-modified iron-loaded ACF (A@Fe-ACF) was obtained by modifying ACF through a two-step impregnation method. After the modification, the oxygen-containing functional groups on ACF increased and provided more adsorption sites. The electron transfer efficiency on the A@Fe-ACF was increased by introducing Fe and synergistically promoted the adsorption of phosphorus and fluorine. Results showed that the removal efficiencies of total phosphorus (TP) and total fluorine (TF) in wastewater reached 89.4% and 85% under optimal conditions (voltage intensity 1.5 V, pH 7, plate spacing 1 cm), while the adsorption mechanism of phosphorus and fluorine was dominated by chemical adsorption. Meanwhile, A@Fe-ACF electrode has good recyclability and stability after five cycles.

Application Potential of Constructed Wetlands on Different Operation Mode for Biologically Pre-Treatment of Rural Domestic Wastewater

In order to satisfy the requirements of rural domestic sewage, a bio-ecological combination system was proposed, including a biological treatment section (anaerobic hydrolysis tank and aerobic tank) and an ecological post-treatment section. This study observed the application potential of constructed wetlands (CW) on different operation modes for biologically pre-treated rural domestic wastewater. The organics and nutrient removal efficiency of the tidal flow constructed wetland (TFCW) and the horizontal subsurface flow constructed wetland (HFCW) were compared at a temperature range of 20–40 °C. During the stable phase, the higher chemical oxygen demand (COD), ammonia nitrogen (NH4+-N), and total phosphorus (TP) removal efficiencies existed in TFCW than HFCW, corresponding to the efficiency of COD 69.46%, NH4+-N 96.47%, and TP 57.38%, but lower performance on COD (61.43%), NH4+-N (84.99%), and TP (46.75%) removal in HFCW, which should be attributed to the increasement of aerobic heterotrophic bacteria (Arthrobact and Sphingomonas), nitrifiers (Nitrospira), and phosphate accumulating organisms (PAOs) (Pseudomonas). The microbial biomass was also increased from 2.13 ± 0.14 mg/g (HFCW) to 4.64 ± 0.18 mg/g (TFCW), which proved to strengthen the formation and growth of biofilm under a better oxygen supplement. Based on the relative abundance of functional genera in the microbial community, it showed that TFCW was more favorable for promoting the growth of heterotrophic bacteria, nitrifiers, and phosphate-accumulating organisms (PAOs). When temperature changed from −4 °C to 15 °C, the two-stage constructed wetlands (TFCW-HFCW and HFCW-TFCW) were used for improving the performance of pollutants removal. The results demonstrated that the effluent concentrations of TFCW-HFCW and HFCW-TFCW met the Class 1A discharge standard of DB32/3462-2020 in JiangSu Province, China. Therefore, this study will provide a useful and easy-to-implement technology for the operation as an ecological post-treatment section.

Towards advanced nutrient removal by microalgae-bacteria symbiosis system for wastewater treatment

In this study, the microalgae-bacteria symbiosis (ABS) system by co-culturing Chlorella sorokiniana with activated sludge was constructed for pollutants removal, and the according interaction mechanism was investigated. The results showed that the ABS system could almost completely remove ammonia nitrogen, and the removal efficiency of total nitrogen and total phosphorus could accordingly reach up to 65.3 % and 42.6 %. Brevundimonas greatly promoted microalgal biomass growth (maximum chlorophyll-a concentration of 9.4 mg/L), and microalgae contributed to the increase in the abundance of Dokdonella and Thermomonas in ABS system, thus facilitating nitrogen removal. The extended Derjaguin-Landau-Verwey-Overbeek theory indicated a repulsive potential barrier of 561.7 KT, while tryptophan-like proteins and tyrosine-like proteins were key extracellular polymeric substances for the formation of flocs by microalgae and activated sludge. These findings provide an in-depth understanding of interaction mechanism between microalgae and activated sludge for the removal of contaminants from wastewater.

Application of iron–carbon microbial galvanic activated sludge combined with MBR process in the treatment of wastewater from comprehensive railway station

The application of iron–carbon microbial cell activated sludge (ICMC-AS) was carried out in a membrane bioreactor (MBR) processor to treat wastewater from an integrated railway station. Results showed that the chemical oxygen demand (COD), total nitrogen (TN), and total phosphorus (TP) removal efficiencies of the original MBR processor increased from 80%, 30%, and 10% to 92%, 93.5%, and 92%, respectively. Further research showed that the combined sewage treatment system also had a strong impact resistance ability. The combined sewage treatment system ran stably when the COD, TN, and TP concentrations changed greatly. The in-depth analysis of the reaction process and reaction rate of the combined sewage treatment system revealed that the combined system is dominated by COD removal with high nitrogen removal efficiency. The removal rate constants of various pollutants were in the order: KCOD (0.647 ± 0.017) > KNO3−−N (0.416 ± 0.044) > KNH4+−N (0.275 ± 0.014) > KTN (0.258 ± 0.083). Calculations of the energy saving and carbon emission reduction of the combined system showed that the system’s annual carbon emission reduction could reach more than 388,203.55 kg CO2e, which remarkably improves the carbon emission reduction effect and obtains a good green effect. The results indicate that adding ICMC-AS to the MBR processor for combined wastewater treatment can substantially improve the efficiency of wastewater treatment and obtain better energy-saving and emission-reducing effects. This combined application provides an effective way for the transformation and upgrading of small- and medium-scale water treatment systems.

Nutrient removal from biogas slurry and biogas upgrading by microalgae-fungi-bacteria co-cultivation under different carbon nanotubes concentration.

In this study, Chlorella vulgaris, Ganoderma lucidum, and endophytic bacteria were co-cultivated with the stimulation of strigolactone analogs GR24 to prepare pellets. During the purification of biogas slurry and biogas, multi-walled carbon nanotubes (MWCNTs) were introduced to enhance the removal efficiencies of nutrients and CO2. The results showed that both GR24 and MWCNTs affected the purification of biogas slurry and biogas. The maximum chemical oxygen demand, total nitrogen, total phosphorus, and CO2 removal efficiencies of the Chlorella vulgaris-Ganoderma lucidum-endophytic bacterial symbionts were 82.57 ± 7.96% (P < 0.05), 82.14 ± 7.87% (P < 0.05), 84.27 ± 7.96% (P < 0.05), and 63.93 ± 6.22% (P < 0.05), respectively, with the induction of 10-9M GR24 and 1mg L-1 MWCNTs. Moreover, the growth and photosynthetic performance of the symbionts were consistent with the removal effects. The Chlorella vulgaris-Ganoderma lucidum-endophytic bacterial symbionts obtained high growth rates and enzyme activity with the maximum growth rate of 0.365 ± 0.03d-1, mean daily productivity of 0.182 ± 0.016g L-1 d-1, and carbonic anhydrase activity of 31.07 ± 2.75 units, respectively. These results indicated that an appropriate concentration of GR24 and MWCNTs could promote the growth of symbionts, reinforce the purification effects of biogas slurry and biogas, and provide a new idea for the simultaneous purification of wastewater and biogas.

Investigation of electrochemical properties, leachate purification, organic matter characteristics, and microbial diversity in a sludge treatment wetland- microbial fuel cell

Sludge treatment wetland-microbial fuel cell (STW-MFC) is a unique sludge treatment process that produces bioelectricity, but its technology is still in its infancy. This study investigated the electrochemical properties, organic matter characteristics, leachate purification, and microbial community structure of STW-MFCs as affected by electrode location. When electrodes were placed in the filler layer, the STW-MFC system presented a higher power generation capacity (maximum output power density: 0.498 W/m3; peak cell voltage: 0.879 V) and organic matter degradation efficiency. The hydrophilic fraction was the main dissolved organic carbon fraction in sludge extracellular biological organic matter (EBOM) and leachate dissolved organic matter (DOM). Aromatics were mainly concentrated in the hydrophobic acid fraction. The UV-254 content of sludge EBOM decreased mainly in the hydrophilic and transphilic acid fractions. The excitation-emission matrix analysis showed that tryptophan-like protein was more easily eliminated than tyrosine-like protein. In addition, there was a strong correlation between voltage and NH4+ removal efficiency; a negative correlation between total chemical oxygen demand (TCOD), total nitrogen (TN), and total phosphorus (TP) removal efficiency, and a negative correlation between pH and TN, TP, and NH4+ removal efficiencies. High-throughput sequencing showed that the system was most abundant in Thermomonas, Geothrix and Geobacter when the electrodes were placed in the filled layer, while the levels of genes for membrane transport, carbohydrate metabolism and energy metabolism functions were higher than in other systems. This work will support STW- MFC widespread implementation by illuminating the underlying mechanics of different anode positions.

Application of Modified Biochar in the Treatment of Pesticide Wastewater by Constructed Wetland

To explore the synergistic effects of modified biochar in the purification of herbicide-containing wastewater, the effect of biochar addition on the removal effect of the herbicide atrazine in wastewater was verified by the addition of biochar bags in a small reed bed-constructed wetland in the laboratory. The results showed that the addition of sulfuric acid-modified biochar could increase the removal rate of atrazine in wastewater from 50% to 70%, and the COD elimination rate in wastewater was from 66.7% to 86.7%. The addition of biochar to the constructed reed bed wetland improved the removal efficiency of total nitrogen and total phosphorus in the wastewater, and the outlet water from the constructed wetland reached the Class III level of China’s surface water quality standard (the inlet water was inferior to Class V). The experimental design met the requirements of low-cost, generalized atrazine-containing wastewater treatment and thus could have the potential for wide application. The results reflected the application potential of modified biochar as a synergist in the treatment of herbicide wastewater in constructed wetlands.

Comparison of Nitrogen and Phosphorus Removal between Two Typical Processes under Low Temperature in a Full-Scale Municipal Wastewater Treatment Plant

Given its strict discharge standards for wastewater treatment plants (WWTPs), China focuses on improving the removal effect of nitrogen and phosphorus in biological treatment processes under low temperatures. The variations in nitrogen and phosphorus during the anaerobic–anoxic-oxic (AAO) and AAO-sequencing batch reactor (SBR) processes in a full-scale WWTP were compared by sampling. Results showed that the removal efficiencies of total nitrogen (TN) and total phosphorus (TP) in both processes exceeded 85% and 91%, respectively, when the water temperature was lower than 15 °C. The wastewater treatment potential capacity of the AAO process was larger than that of AAO-SBR, indicating that the AAO process could realize the subjective demand of nitrogen or phosphorus removal by adjusting its operation mode. The anaerobic phosphorus release of the AAO process was affected when part of the internal reflux entered the anaerobic tank. Thus, the biological phosphorus removal of the AAO process was worse than that of the AAO-SBR process. Since the nitrification and denitrification rates of the AAO process were all higher than that of the AAO-SBR process, the TN removal efficiency of AAO was higher than that of AAO-SBR. These results could provide some advice for the upgrade, operation optimization, and process selection of both processes in the future.