Dentin Remineralization Research Articles

Remineralisation of mineral-deficient dentine induced by experimental ion-releasing materials in combination with a biomimetic dual-analogue primer

ObjectivesEvaluate the remineralisation ability of experimental resin-based materials containing Fluoride-Doped Calcium Phosphate (FDCP), applied in mineral-deficient dentine in combination with a biomimetic dual-analogue primer. MethodsArtificial dentine carious lesions were created in occlusal cavities of human molars. An experimental resin-based adhesive and flowable composite, containing FDCP (10wt% and 20wt%, respectively), were applied (±) with a biomimetic primer containing polyacrylic acid and sodium tripolyphosphate. A glass-ionomer cement and a conventional adhesive/composite were used as comparative materials. All specimens were submitted to chewing simulation in artificial saliva (AS). Subsequently, these were cut into slabs and submitted to Knoop microhardness testing and Raman spectroscopy (AS: 0, 15, 30 and 60 days). Further specimens were analysed through confocal scanning microscopy (AS: 0, 60 days). Total demineralised dentine and disc-shape materials were analysed through FTIR, SEM and EDX (Ca/P ratio) after prolonged contact in AS. Data were statistically analysed (two-way ANOVA and multiple comparison Bonferroni; α=0.05). ResultsOnly the experimental FDCP materials induced a significant increase in microhardness after storage in AS, especially in those specimens pre-treated with the biomimetic primer, except at 50 µm underneath the interface. Raman and CLSM analysis corroborated such results. The ability of the experimental FDCP resin-based materials to induce apatite-like deposition was demonstrated through SEM-EDX and FTIR. ConclusionsResin-based materials containing FDCP can mineralise the mineral-deficient dentine, especially when combined with a biomimetic dual-analogue primer. Clinical SignificanceSuch Innovative ion-releasing restorative systems applied after selective caries removal might promote remineralisation of fully and partially demineralised dentine and increase the durability of dental restorations.

Influence of Phase Composition and Morphology on the Calcium Ion Release of Several Classical and Hybrid Endodontic Cements.

The ability of the cement to release calcium ions, which participate in the remineralization of dentin by forming apatite which improves root canal sealing with time, is of particular importance. Five recently introduced calcium-silicate commercial dental cements were investigated with a view to the influence of the physicochemical characteristics on the possibility of releasing calcium ions in an aqueous medium. Two hybrid calcium-silicate cements in the form of a paste-like ready mix (BioCal® Cap and TheraCal LC) and three calcium-silicate cements consisting of two components-powder and liquid (Harvard MTA Universal, Rootdent, and BioFactor) were subjected to powder XRD, SEM, and EDS for detailed examination. The cements were immersed in water for 28 days and the phase composition and morphology of the cements before and after soaking were studied. The total calcium release for each cement was determined by ICP-OES. BioFactor and BioCal® Cap release the highest amount of calcium ions, while the lowest release is registered with Rootdent and TheraCal LC. The PDT treatment of BioFactor does not influence substantially the calcium release. The impact of the elemental and phase composition on the calcium release and calcium carbonate formation was discussed. A reciprocal relation between the aluminum content and the quantity of the released calcium has been found.

Effects of Silver Diamine Fluoride and Potassium Iodide Solutions on the Anti-Caries Properties, Surface Hardness, and Bond Strength of Resin Modified Glass Ionomer in Dentin

This study evaluated the effects of silver diamine fluoride (SDF) and potassium iodide (PI) on carious dentin. The antibacterial efficacy, surface hardness, and bond strength of resin-modified glass ionomer cement (RMGI) were assessed. Dentin specimens were prepared and subjected to artificial caries formation. The antibacterial effect of SDF/PI was compared to sodium fluoride varnish using Streptococcus mutans colony counts. Surface microhardness was measured using the Vickers hardness test. Shear bond strength of RMGI to treated surfaces was evaluated after thermocycling. Results showed that SDF/PI had superior antibacterial effects compared to NaF varnish. Surface hardness significantly increased one week after SDF/PI application. While SDF/PI treatment did not immediately affect RMGI bond strength to carious dentin, delayed bonding after one week significantly improved bond strength. These findings suggest that SDF/PI is effective in arresting caries and enhancing remineralization of carious dentin. The study also indicates that delaying restoration placement for one week after SDF/PI application may be beneficial for improving bond strength. This research provides valuable insights into the clinical application of SDF/PI for caries management and subsequent restorative procedures.

Dentinal Tubule Occlusion and Dentin Permeability Efficacy of Silver Diamine Fluoride Solutions.

This invitro study compared the effect of two commercially available silver diamine fluoride (SDF) products on dentinal tubule (DT) occlusion and dentin permeability. Sixty-six dentin discs (1.0 mm thick) were divided equally into six groups: acid-etched with no treatment (negative control-NC), etched and sealed with nail varnish (positive control-PC), non-etched and treated with Advantage Arrest (AA), non-etched and treated with Topamine (TP), etched and treated with AA, and etched and treated with TP. Eight specimens from each group were tested for permeability using the dye percolation method. The remaining three samples from each group were assessed for dentinal tubule occlusion, depth of SDF penetration, and elemental analysis using a scanning electron microscope coupled with an energy-dispersive X-ray (SEM/EDX) system. One-way ANOVA and Tukey's post hoc test were used to analyze the results. TP exhibited lower (p < 0.05) dye percolation compared to AA. While both SDF materials showed a statistically similar (p > 0.05) penetration depth in the DT, none of them had the ability to completely occlude all the DTs. AA exhibited more silver and fluoride ion deposition on etched dentin than TP, and both materials showed a similar increase in the deposition of Ca and P compared to NC. Whereas the effectiveness of dentin tubule (DT) occlusion varies based on the material used, both tested SDF products exhibit the capability to seal dentinal tubules, decrease dentin permeability, and promote dentin remineralization. These findings suggest their potential advantage in managing dentin hypersensitivity.

Effects of glutathione and potassium iodide on silver diamine fluoride application on remineralisation and colour change in dentine caries of primary teeth: an in vitro study.

The aim of this study is to compare the effect of GSH (reduced glutathione) and KI (potassium iodide) on SDF (silver diamine fluoride) discolouration and dentine remineralisation. Sixteen primary molars were utilised, yielding 4 dentine specimens each. Three specimens per tooth were allocated: one as a control and the others to experimental groups. Initial microhardness measurements were taken from one remaining dentine specimen per tooth. Subsequently, all groups underwent exposure to a demineralisation solution. Colorimetry assessed specimen colour, and post-second microhardness measurements on demineralised specimens, treatments were administered as follows: group 1 (control, n = 16): 38% SDF, group 2 (n = 16): 38% SDF followed by KI, group 3 (n = 16): 38% SDF with 5% GSH added by weight. Following pH cycling across all groups, colorimetry reassessed 48 dentine specimens. Final microhardness measurements ensued, followed by statistical analysis. Normality was checked via Shapiro-Wilk, and homogeneity via Levene's test. Independent samples t test compared normally distributed groups; Mann-Whitney U compared non-normally distributed groups. ANOVA compared means of normally distributed groups, and Kruskal-Wallis for non-normally distributed ones. Repeated measures ANOVA compared dependent groups with normal distribution, and Friedman test for non-normal. Post hoc Bonferroni analyses identified significant differences. IBM SPSS 25 was used to conduct analyses. The mean ΔE* values for SDF and SDF + GSH groups were significantly higher than those of the SDF + KI group (p < 0.05). Significant differences in L* values during final colour measurement were noted between the SDF + KI group and both SDF and SDF + GSH groups (p < 0.05). Although mean remineralisation microhardness measurements were higher than mean demineralisation microhardness measurements in all groups, statistical significance was observed only in the SDF and SDF + KI groups (p < 0.05). The study found that the addition of 5% GSH by weight to SDF does not significantly affect discolouration. Moreover, the addition of 5% GSH to the SDF solution may have a minor impact on the remineralisation potential of SDF. The application of KI after SDF reduces discolouration and does not affect the expected remineralisation process.

Hagfish-inspired hydrogel for root caries: A multifunctional approach including immediate protection, antimicrobial phototherapy, and remineralization

Root caries is the main cause of oral pain and tooth loss in the elderly. Protecting root lesions from environmental disturbances, resisting pathogens, and facilitating remineralization over time are essential for addressing root caries, but are challenging due to the irregular root surface and the complex oral environment. Hagfish secretes slime when facing danger, which converts into gels upon contact with seawater, suffocating the predators. Inspired by hagfish's defense mechanism, a fluid-hydrogel conversion strategy is proposed to establish a mechanical self-regulating multifunctional platform for root caries treatment. The fluid system (silk fibroin-tannic acid-black phosphorene-urea, ST-BP-U), in which urea disrupts the hydrogen bonds between silk fibroin and tannic acid, can easily spread on the irregular root surface and permeate into dentinal tubules. Upon contact with the surrounding water, urea diffuses, prompting the hydrogel re-formation and creating intimate attachments with micromechanical inlay locks. Meanwhile, BP increases the crosslinking of the re-formed hydrogel network, resulting in reinforced cohesion for robust wet adhesion to the tooth root. This process establishes a structured platform for effective antimicrobial phototherapy and dentin remineralization promotion. This water-responsive fluid-hydrogel conversion system adapts to the irregular root surface in the dynamic wet environment, holding promise for addressing root caries. Statement of significanceRoot caries bring a heavy burden to the aging society, but the irregular root surface and dynamic moist oral environment always hinder non-surgical therapeutic effects. Here, we propose a water-responsive fluid-hydrogel conversion strategy aimed at mechanical self-regulation on the irregular and wet root interface to construct a functional structural platform. The liquid system (ST-BP-U) that prebreak intermolecular hydrogen bonds can easily spread on irregular surfaces and dentin tubules. When encountering water, hydrogen bonds re-form, and BP increases the crosslinking of the hydrogel formed in situ. Based on this firm wet-adhesion platform, it provides powerful phototherapy effects and promotes dentin remineralization. This fluid-hydrogel conversion system turns the disadvantages of wet environment into advantages, offering a promising strategy for root caries.

Promotion effect of proanthocyanidin on dentin remineralization via the polymer induced liquid precursor process

Proanthocyanidin (PA) has demonstrated promise as a dental biomodifier for maintaining dentin collagen integrity, yet there is limited evidence regarding its efficacy in dentin repair. The aim of this study was to investigate the effect of PA on dentin remineralization through the polymer induced liquid precursor (PILP) process, as well as to assess the mechanical properties of the restored dentin. Demineralized dentin was treated with a PA-contained remineralization medium, resulting in the formation of PA-amorphous calcium phosphate (ACP) nanoparticles via the PILP process. The kinetics and microstructure of remineralized dentin were examined through the use of Fourier transform infrared spectroscopy(FTIR), attenuated total reflectance-FTIR, scanning electron microscopy, transmission electron microscopy. The results showed that the application of PA facilitated the process of dentin remineralization, achieving completion within 48 h, demonstrating a notable reduction in time required. Following remineralization, the mechanical properties of the dentin exhibited an elastic modulus of 15.89 ± 1.70 GPa and a hardness of 0.47 ± 0.08 GPa, which were similar to those of natural dentin. These findings suggest that combining PA with the PILP process can promote dentin remineralization and improve its mechanical properties, offering a promising new approach for dentin repair in clinical practice.

Effect of the anti-Alzheimer drug GSK-3β antagonist on numerical modeling of the energy dissipation through the resin-dentin interface

ObjectivesThe aim of this study was to determine the viscoelastic performance and energy dissipation of conditioned dentin infiltrated with polymeric nanoparticles (NPs) doped with tideglusib (TDg) (TDg-NPs). MethodsDentin conditioned surfaces were infiltrated with NPs and TDg-NPs. Bonded interfaces were created, stored for 24 h and submitted to mechanical and thermal challenging. Resin-dentin interfaces were evaluated through nano-DMA/complex-loss-storage moduli-tan delta assessment and atomic force microscopy (AFM) analysis. ResultsDentin infiltrated with NPs and load cycled attained the highest complex modulus at hybrid layer and bottom of hybrid layer. Intertubular dentin treated with undoped NPs showed higher complex modulus than peritubular dentin, after load cycling, provoking energy concentration and breakdown at the interface. After infiltrating with TDg-NPs, complex modulus was similar between peri-intertubular dentin and energy dissipated homogeneously. Tan delta at intertubular dentin was higher than at peritubular dentin, after using TDg-NPs and load cycling. This generated the widest bandwidth of the collagen fibrils and bridge-like mineral structures that, as sight of energy dissipation, fastened active dentin remodeling. TDg-NPs inducted scarce mineralization after thermo-cycling, but these bridging processes limited breakdown zones at the interface. SignificanceTDg-based NPs are then proposed for effective dentin remineralization and tubular seal, from a viscoelastic approach.

A bifunctional lactoferrin-derived amyloid coating prevents bacterial adhesion and occludes dentinal tubules via deep remineralization

Dentin hypersensitivity (DH) manifests as sharp and uncomfortable pain due to the exposure of dentinal tubules (DTs) following the erosion of tooth enamel. Desensitizing agents commonly used in clinical practice have limitations such as limited depth of penetration, slow remineralization and no antimicrobial properties. To alleviate these challenges, our study designed a lactoferrin-derived amyloid nanofilm (PTLF nanofilm) inspired by the saliva-acquired membrane (SAP). The nanofilm utilises Tris(2-carboxyethyl)phosphine (TCEP) to disrupt the disulfide bonds of lactoferrin (LF) under physiological conditions. The PTLF nanofilm modifies surfaces across various substrates and effectively prevents the early and stable adhesion of cariogenic bacteria, such as Streptococcus mutans and Lactobacillus acidophilus. Simultaneously, it adheres rapidly and securely to demineralized dentin surfaces, facilitating in-situ remineralization of HAP through a simple immersion process. This leads to the formation of a remineralized layer resembling natural dentin, with an occlusion depth of dentinal tubules exceeding 80 µm after three days. The in vivo and vitro results confirm that the PTLF nanofilm possesses good biocompatibility and its ability to exert simultaneous antimicrobial effects and dentin remineralization. Accordingly, this innovative bifunctional PTLF amyloid coating offers promising prospects for the management of DH-related conditions. Statement of significance1.We design a simple, fast, inexpensive, and easy-to-process PTLF nanofilm for nearly any material surface or shape.2.The PTLF nanofilm modifies surfaces across various substrates and effectively prevents the adhesion of cariogenic bacteria, such as Streptococcus mutans and Lactobacillus acidophilus.3.The abundant functional groups on the surface of PTLF nanofilm facilitate bioactive hydroxyapatite (HAP) formation and maintain stability at the HAP remineralization interface.

Evaluation of Surface Topography and Biomimetic Remineralization Capacity of Dendrimers in Comparison With Calcium Silicate Cement: An In Vitro Study.

Biodentine, a calcium silicate-based material, is known for its biocompatibility and ability to promote dentin regeneration. With their unique branching structure, polyamidoamine (PAMAM) dendrimers have shown promise in facilitating biomimetic remineralization processes. This study investigates the synergistic effects of combining PAMAM with Biodentine on root dentin remineralization, aiming to develop a novel bioactive compound that offers superior protective and regenerative properties. The following predictions were made: (1) In a cyclic artificial saliva/acid regimen, among the test groups, the combination of Biodentine and PAMAM would cause the most root dentin remineralization (2). Biodentine alone would increase Ca and P concentrations, neutralize acid, and promote root dentin remineralization (3). PAMAM, on the other hand, can remineralize the demineralized root dentin. Minimal mineral regeneration was accomplished in demineralized root dentin when treated with Biodentine or PAMAM alone. Root dentin remineralization was most pronounced when Biodentine and PAMAM were used together, and the hardness of demineralized root dentin was raised to an equivalent level to that of healthy root dentin. The study demonstrated the exceptional ability of PAMAM + Biodentine to promote root dentin remineralization. In an acid-challenging environment, PAMAM + Biodentine promoted full and efficient root dentin remineralization. Restorations made using innovative PAMAM + Biodentine technology show promise in remineralizing and protecting tooth structures.

Evaluation of Physical Properties in Carboxymethyl Chitosan Modified Glass Ionomer Cements and the Effect for Dentin Remineralization: SEM/EDX, Compressive Strength, and Ca/P Ratio.

The aim of this article was to evaluate the effects of modifying glass ionomer cement (GIC) with carboxymethyl chitosan (CMC) on surface morphology and remineralization outcomes by examining dentin morphology and calcium ion composition changes. Thirty holes in a cylindrical acrylic mold were filled with three groups of restorative materials: GIC, GIC modified with CMC (GIC-CMC) 5%, and GIC-CMC10%. The surface morphology of each group's materials was observed using scanning electron microscopy (SEM). The compressive strength measurement was performed using a universal testing machine. The dentin remineralization process was performed by applying GIC, GIC-CMC5%, and GIC-CMC10% materials for 14 days on demineralized dentin cavities treated with 17% ethylenediamine tetraacetic acid (EDTA) for 7 days. A morphological evaluation was conducted using SEM. The calcium ion composition and calcium-to-phosphorous (Ca/P) ratio were examined using an energy-dispersive X-ray (EDX). The Kruskal-Wallis and post-hoc Mann-Whitney U tests were performed to compare all four groups of calcium ions (p < 0.05). The modification of GIC with CMC affected the morphological changes in the materials in the form of reduced porosity and increased fractures. A significant difference was found in compressive strength between the GIC-CMC modification materials of GIC-CMC5% and GIC-CMC10% and the GIC control group. The dentin tubule morphology and surface changes were observed after applying GIC, GIC-CMC5%, and GIC-CMC10% materials for 14 days, as evaluated by SEM. The EDX examination showed an increase in calcium ion content and hydroxyapatite formation (Ca/P ratio) after applying the GIC-CMC10% material. The surface porosity of the GIC modification material with the addition of CMC tended to decrease. However, an increase in cracked surfaces that widened, along with the rise in CMC percentage, was found. This modification also reduced the compressive strength of the materials, with the lowest average yield at 10% CMC addition. Therefore, the modification of GIC with CMC affects changes in morphology, calcium ion composition, and Ca/P ratio in demineralized dentin.

Comparison of remineralization ability of tricalcium silicate and of glass ionomer cement on residual dentin: an in vitro study

ObjectiveThis study aimed to compare the remineralization effects of a calcium silicate-based cement (Biodentine) and of a glass ionomer cement (GIC: Fuji IX) on artificially demineralized dentin.MethodsFour standard cavities were prepared in dentin discs prepared from 34 extracted sound human third molars. In each disc, one cavity was covered with an acid-resistant varnish before demineralization (Group 1). The specimens were soaked in a chemical demineralization solution for 96 h to induce artificial carious lesions. Thereafter, one cavity each was filled with Biodentine (Group 2) and GIC (Group 3), respectively, and one carious lesion was left unrestored as a negative control (Group 4). Next, specimens were immersed in simulated body fluid (SBF) for 21 days. After cross-sectioning the specimens, the Ca/P ratio was calculated in each specimen by using scanning electron microscopy–energy-dispersive X-ray spectroscopy (SEM-EDX). Finally, data were analyzed using repeated-measures ANOVA with post-hoc Bonferroni correction.ResultsBoth cement types induced dentin remineralization as compared to Group 4. The Ca/P ratio was significantly higher in Group 2 than in Group 3 (p < 0.05).ConclusionThe dentin lesion remineralization capability of Biodentine is higher than that of GIC, suggesting the usefulness of the former as a bioactive dentin replacement material.Clinical relevanceBiodentine has a higher remineralization ability than that of GIC for carious dentin, and its interfacial properties make it a promising bioactive dentin restorative material.

Bond Strength of Resin Cement Following Biomimetic Remineralization: An in vitro Study

Objectives This research assessed the efficacy of self-assembling peptide P11-4 (Curodont protect) in promoting biomimetic remineralization of dentin, thereby stabilizing the hybrid layer and enhancing the long-lasting of the resin-dentin bond. Methods Five premolar teeth were longitudinally sectioned to assess dentin microhardness before and after demineralization, as well as after the application of P11-4 (Curodont protect). For the microtensile test, ten premolar teeth were sectioned perpendicular to their long axes. The teeth were assigned to different groups. Following acid etching and rinsing, samples in control group I received no pretreatment, while the prepared dentin surface of group II was treated with 0.1 mL of P11-4 before the placement of the adhesive restoration. All specimens were stored in distilled water at 37 ± 2 °C for 24 hours and then underwent thermocycling. The microtensile test was measured, and the type of bond failure was evaluated. The obtained information, expressed in megapascals, was analyzed using a one-way analysis of variance (ANOVA), followed by Tukey’s multiple post hoc test. Results There was a statistically significant difference in hardness values after demineralization, followed by a significant increase in hardness after the application of P11-4 (Curodont protect) (p &lt; 0.001, effect size = 0.985). No statistically significant difference was found in the microtensile bond strength between the two groups (p = 0.384, effect size = 0.582). Adhesive-type failure was more commonly observed. Conclusion The use of self-assembling peptide P11-4 (Curodont protect) on dentin resulted in higher microhardness than the control group without adversely affecting resin cement bond strength.

Tunable polymer-peptide hybrids for dentin tissue repair

ObjectivesThis study targets to assess the remineralization capability of conditioned dentin infiltrated with polymeric nanoparticles (NPs) doped with tideglusib (TDg) (TDg-NPs). MethodsDentin conditioned surfaces were infiltrated with NPs and TDg-NPs. Bonded interfaces were created, stored for 24 h and submitted to mechanical and thermal challenging. Resin-dentin interfaces were evaluated through nanohardness, Masson's trichrome staining microscopy, and Raman analysis. ResultsDentin surfaces treated with TDg-NPs and load cycled produced higher nanohardness than the rest of the groups at the hybrid layer. At the bottom of the hybrid layer, all samples treated with TDg-NPs showed higher nanohardness than the rest of the groups. Active remineralization underneath the hybrid layer was detected in all groups after TDg application and load cycling, inducting new dentinal tubuli formation. After thermocycling, remineralization at the hybrid layer was not evidenced in the absence of NPs. Raman analysis showed increase mineralization, enriched carbonate apatite formation, and improved crosslinking and scaffolding of the collagen. ConclusionsMechanical loading on the specimens obtained after TDg-NPs dentin infiltration inducts an increase of mineralization at the resin/dentin interface, indicating remineralization of peritubular and intertubular dentin with augmented crystallographic maturity in crystals. Enriched collagen quality was produced, generating an adequate matrix organization to promote apatite nucleation, after tideglusib infiltration. Clinical significanceAt the present research, it has been proved the creation of reparative dentin, at the resin-dentin interface, after tideglusib dentin infiltration. Chemical stability, to favor integrity of the resin-dentin interface, is warranted in the presence of the TDg-NPs in the demineralized dentin collagen.

The Synergism of Silver and Fluoride: A New Arsenal in Caries Management

AbstractTopical anticaries agents are non‐invasive methods to control and prevent dental caries. SDF is a fluoride‐based agent added to the arsenal of caries prevention tools. It has got a lot of attention since its introduction in the 1970s because of its ability to prevent and arrest caries, as well as its excellent antibacterial and anti‐plaque action. SDF reduces acid production by microorganisms, decreases the Streptococcus mutans count, biofilm formation, increases enamel resistance, and promotes dentinal tubule obliteration when applied to the dental surface. SDF also assists in the remineralization of dentin. A biannual application of 38% SDF has been effective at preventing dentin demineralization. This “silver bullet” can be seen as an alternative for caries prevention in special health care needs children which is minimally invasive and safe. Along with the advantages, there are some disadvantages, and work has been carried out to explore the use of silver nanoparticles (SNP) as anticaries agents to shield the teeth from aesthetic damage caused by Silver diamine fluoride. SNPs are identified to have the potential to prevent and inhibit dental caries while not discoloring demineralized dental enamel. SNPs have a greater surface area for interaction with microorganisms than other particles, making them more effective antimicrobial agents. Fluoride combined with SNP solution has a synergistic effect that promotes enamel remineralization while also acting as a bactericidal agent against cariogenic microorganisms. This review aims to bring out the various advantages and disadvantages related to the use of SDF and SNPs to aid in future research.

Komposit Bioactive Glass Berbasis Silika Abu Ampas Tebu dan Gum Mimba Menstimulasi Proses Remineralisasi Permukaan Gigi

Dentin hypersensitivity results from exposed dentinal tubules. One of the treatments used is remineralization of dentin and cementum. Bioactive glass (BAG) is a material that can stimulate the remineralization process due to its silica content. Sugarcane bagasse contains high levels of silica and is used as a BAG material. Adding binders and emulsifiers to paste forms increases bonding and stability and makes application easier. Neem gum is a polysaccharide, has emulsifying properties, and can unite two or more ingredients with different properties, increasing the strength of the ingredients. The study aims to analyse the effect of neem gum and bioactive glass composites based on silica bagasse ash on tooth remineralisation. There were three research groups, namely control (BAG paste), treatment A (hyaluronic acid BAG paste) and treatment B (BAG paste, hyaluronic acid and neem gum). The injection paste was applied to the teeth [using cow teeth] on the mesial surface, then soaked in artificial saliva for six days and stored in an incubator at 370C. The remineralization process was observed by forming hydroxycarbonate apatite (HCA) on the tooth root surface using a scoring method based on the Scanning Electron Microscope (SEM) results. CA formation in the BAG, hyaluronic acid and neem gum composite group had a significantly (p&lt;0.05) higher HCA formation score than the group without gum. The control group experienced a washout, so no HCA was formed. BAG composite paste with silica based on bagasse ash and neem gum stimulates the remineralization process marked by the formation of HCA on the tooth root surface.

Correlating immediate vapour reactivity with dentin remineralization and tubule occlusion capacity of a bioactive sol-gel borate glass

The use of bioactive glass as a mineralization agent in treating dentin hypersensitivity has been established for almost two decades where the melt-quench-derived silicate glass, 45S5, may be regarded as the gold standard formulation. Compared to silicate glasses, borate glasses are less chemically durable and have the potential to mineralize (i.e., convert to apatite) more rapidly. This study compared the apatite forming capacity of a sol-gel-derived calcium-borate glass, 40CaO–60B2O3 (SGB60), to that of 45S5, on demineralized dentin discs and its ability to occlude tubules on non-demineralized dentin discs, in vitro. Moreover, it investigated the vapour sorption reactivity of the glasses to explore the potential correlation between their immediate aqueous interactions and bioactivity in remineralization solution. Compared to 45S5, SGB60 demonstrated more rapid calcium-phosphate layer formation on demineralized dentin surfaces. On the other hand, after 24 h immersion in remineralization solution, the 45S5-induced calcium-phosphate layer occlusion of tubules in non-demineralized dentin surfaces appeared to be qualitatively greater than that induced by SGB60. Moreover, vapour sorption analysis under either air or nitrogen gas, demonstrated that the reactivity of 45S5 with vapour varied under the different purge gasses, with significant carbonate formation under air. In contrast, SGB60 showed a more rapid rate of initial weight increase with vapour under both purge gasses, and this vapour reactivity may be correlated to its more rapid calcium-phosphate layer formation on demineralized dentin surfaces when immersed in remineralization solution.

Comparing Fluoride Varnish, Fissure Sealant and Ozone for Children Caries Prevention

Background: Caries is the most prevalent oral health disease in children globally. Well-known method such as fluoride varnish and fissure sealant has been used to prevent caries overtime. Ozone therapy considered as a new innovation in caries prevention for children which has not been studied much for its effectiveness compared to the other popular method. Objective: This review aimed to compare caries preventive methods with fluoride varnish (FV), fissure sealant (FS) and ozone (O) for children and decide which method is the most effective. Literature Study: PubMed, SpringerLink, EBSCOhost and Science direct were searched from December 2021 to January 2022. The criteria included all randomized controlled trials and clinical trials comparing the caries preventive effect between FV, FS and O on children’s permanent teeth. Discussion: A total of 12 articles were included. Regarding dentin caries prevention, 2 studies found resin FS in favour of no FS and 2 studies found FV in favor of controls. 1 study agreed glass ionomer FS and no FS comparable. 3 studies agreed FV and FS were comparable. 1 study agreed resin FS and glass ionomer FS comparable. 1 study also found FV, FS and O comparable. Regarding dentin remineralization, 2 studies found O were comparable with FS dan calcium hydroxide base. These methods are safe for children. Conclusion: Fluoride varnish, fissure sealant and ozone are effective in preventing children’s caries with their own benefits and drawbacks. None of the methods is superior over another as there were a variety of the data reported.

Remineralisation of enamel and dentine with stabilised stannous fluoride dentifrices in a randomised cross-over in situ trial

ObjectivesTo compare the remineralisation efficacy and ion bioavailability of two novel SnF2-containing dentifrices in a blinded, cross-over, randomised in situ clinical study. MethodsSix participants wore removal palatal appliances holding human enamel and dentine blocks with subsurface lesions. Appliances were worn for two treatment periods of 14 consecutive days each, with a one-week washout period in-between. Participants were randomly allocated to rinse with a 1:5 diluted coded slurry of one of two dentifrices containing either 5 % casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) +1100 ppm F as SnF2 [MIPOP], or 1100 ppm F as SnF2 [CT], for 1 min, four times a day. Saliva was collected post-treatment and analysed for tin, calcium, inorganic phosphate and fluoride ions using atomic absorption spectrophotometry and ion chromatography. Enamel and dentine lesions were analysed for percent remineralisation (%R) using transverse microradiography and percent surface microhardness recovery (%SMHR). ResultsMIPOP released significantly higher F (3.00 ± 0.27 mM), Ca (15.23 ± 3.23 mM) and Sn (1.18 ± 0.13 mM) into saliva whereas CT released 2.89 ± 0.32 mM F and only 0.84 ± 0.11 mM Ca and 0.28 ± 0.10 mM Sn. MIPOP produced significantly higher %R than CT: 25.6 ± 1.5 % compared to 15.2 ± 0.7 % in enamel, and 33.6 ± 3.1 % compared to 20.6 ± 1.1 % in dentine. Additionally, MIPOP produced significantly higher %SMHR (18.2 ± 7.9 %) compared to CT (4.1 ± 0.6 %). ConclusionsBoth dentifrices promoted remineralisation, but the MIPOP dentifrice with added CPP-ACP and the ion-stabilising effects of CPP released higher amounts of bioavailable tin and produced significantly higher remineralisation and surface microhardness recovery. Clinical SignificanceModern dentifrices contain SnF2 for a range of oral health benefits. Challenges associated with stability of these formulations can affect ion bioavailability, reducing efficacy. Two dentifrices with SnF2 promoted remineralisation in situ, however the dentifrice with the added saliva biomimetic CPP-ACP was superior and therefore may produce greater health benefits.

Dentine Remineralisation Induced by “Bioactive” Materials through Mineral Deposition: An In Vitro Study

Dentine Remineralisation Induced by “Bioactive” Materials through Mineral Deposition: An In Vitro Study