A simple, rapid, and reliable HPTLC method has been developed for determination of ethamsylate in tablets. Identification and determination were performed on 10 cm × 20 cm, layer thickness 0.2 mm, aluminum-backed silica gel 60 F 254 HPTLC plates previously washed with methanol. Chloroform-methanol-acetic acid, 2 + 1 + 0.2 ( v/v ), was used as mobile phase. Calibration plots were established showing the dependence of response (peak area) on the amount chromatographed. The validated calibration range was 500–2500 ng per spot ( r = 0.997). The spots were scanned at λ = 300 nm. The suitability of this HPTLC method for quantitative determination of the compound was proved by validation in accordance with the requirements of pharmaceutical regulatory standards. The method was used for determination of the compound in commercial pharmaceutical dosage forms. The method is simple, reproducible, and accurate and is a more effective option than other chromatographic techniques in routine quality control.