In the context of freshwater ecosystems, turbidity and suspended solids play crucial roles, with their levels significantly influenced by anthropogenic activities. This study focuses on assessing and monitoring these parameters in Albufera de Valencia using Sentinel-2 imagery. The primary aim is to establish reliable estimation algorithms that can contribute to effective ecosystem management. This study calibrated and validated algorithms for estimating turbidity and suspended solids. The R783 × R705/R490 model proved to have the best performance for estimating turbidity and total solids in Albufera. However, R783/R490 obtained a higher coefficient of determination for the organic part, while the R705 band was selected for the inorganic part. However, to achieve better estimates of turbidity and inorganic matter, more research is needed in the future. The implications of excessive suspended solids are underscored, including the depletion of dissolved oxygen and reduced primary productivity due to limited light penetration and habitat availability. Collaboration between disciplines such as limnology, optics, and water chemistry is crucial to advance water quality estimation models in lakes and lagoons such as Albufera. By integrating expertise and approaches from these diverse fields, new knowledge can be gained, and the basis for more effective management and conservation strategies can be laid.