We have previously reported the validity and reliability of microelectromechanical system (MEMS) high resolution accelerometers (HRA) relative to VO2 in highly trained and untrained individuals during walking and running. PURPOSE: To determine if MEMS HRA could identify differences in mechanics of running between trained (T) and (UT) runners during treadmill locomotion. METHODS: Procedures were approved by the Eastern Michigan University CHHS Human Subjects Review Committee and subjects gave informed consent. 18 runners, [9 T (21.4±1.7yr, 65.5±5.7 kg, 70.1±6.2 ml/kg/min) and 9 UT (31.6±1.5yr, 69.8±3.7 kg, 49.2±4.2 ml/kg/min)] performed 2 incremental VO2max trials while wearing HRA (Microstrain, VT). Aggregate acceleration for vertical (VT), anterior/posterior (AP), medial/lateral (ML), axes were recorded and Euclidean resultants were computed (RES). Trials consisted of 2 min stages starting at 2kph and increasing 2kph until exhaustion. Group comparisons were made from 8-16 kph, as all subjects completed these stages. Axial root mean square (RMS), RMS relative to speed (EC), and ratio of RMS/RES (RA) were compared between T and UT to determine if differences in running mechanics could be identified between the two groups. Expired gasses were collected using an Oxycon Mobile (Viasys, CA). VO2 and RER, were used to determine O2 cost (O2C) and energy expenditure (EE). RESULTS: Regression of RES was strongly related to VO2, but the regression trend for T was significantly different than UT (p<.001) across running speeds. T exhibited lower RMS and EC than UT in all axes (p <.003). Although MLRA was also lower for T vs UT, VTRA was higher (p <.001). All of these differences while running were despite higher VO2, O2 cost, and lower RER in T vs. UT, indicating greater fat utilization in T. There was no significant difference in energy expenditure between groups. CONCLUSIONS: These results demonstrate that T runners accelerate less in all axes and relative to all speeds than UT, yet allocate a greater proportion of their RES acceleration in the VT axis. These differences in acceleration parameters are not necessarily reflected in lower O2C or EE of running in T vs UT. Therefore, it is unclear if these differences confer a performance advantage in T runners over UT, and the importance of these differences is yet to be determined.