Release Of Riboflavin Research Articles

Responsive porous microneedles with riboflavin ocular microinjection capability for facilitating corneal crosslinking

Riboflavin-5-phosphate (riboflavin) is the most commonly used photosensitizer in corneal crosslinking (CXL); while its efficient delivery into the stroma through the corneal epithelial barrier is challenging. In this paper, we presented novel responsive porous microneedles with ocular microinjection capability to deliver riboflavin controllably inside the cornea to facilitate CXL. The microneedle patch was composed of Poly (N-isopropyl acrylamide) (PNIPAM), graphene oxide (GO), and riboflavin-loaded gelatin. After penetrating the cornea by the stiff and porous gelatin needle tip, the photothermal-responsive characteristic of the PNIPAM/GO hydrogel middle layer could realize the contraction of the gel under the stimulation of near-infrared light, which subsequently could control the release of riboflavin from the backing layer into the cornea stromal site both in vitro and in vivo. Based on the microneedles system, we have demonstrated that this microinjection technique exhibited superior riboflavin delivery capacity and treatment efficacy to the conventional epithelial-on protocol in a rabbit keratoconus model, with benefits including minimal invasiveness and precise administering. Thus, we believe the responsive porous microneedles with riboflavin ocular microinjection capability are promising for clinical corneal crosslinking without epithelial debridement.

Fabrication of mung bean protein-sugar beet pectin hydrogels by duo-induction of heating and laccase: Gelling properties and delivery of riboflavin

Aim of this research was to assess gelling properties and riboflavin delivery capacity of mung bean protein (MBP)-sugar beet pectin (SBP) hydrogels by duo-induction of heat and laccase. The results showed that hydrogel properties were regulated by SBP concentration. When SBP concentration was 0.20%, composite hydrogel had the highest stress (665.27 ± 26.36 Pa), water holding capacity (WHC) (75.68 ± 0.88%), storage modulus (G′) and surface hydrophobicity (H0) (9550.50 ± 33.86) (P < 0.05). At the same time, network structure of composite hydrogel was the densest and system was the most stable. When SBP concentration was 0.20%, composite hydrogel had better swelling rate, riboflavin encapsulation efficiency (51.81 ± 2.31%), cumulative release rate, biological accessibility (37.83 ± 0.90%) and storage stability (P < 0.05) compared with low concentration SBP. The introduction of SBP triggered the transition of riboflavin release mechanism to non-Fick release mechanism and transported riboflavin to the colon effectively. This study might provide theoretical support for the interaction between proteins and polysaccharides in composite hydrogel system as well as provide theoretical basis for composite hydrogel to be a delivery vehicle for bioactive substances.

Photothermal Iron-Based Riboflavin Microneedles for the Treatment of Bacterial Keratitis via Ion Therapy and Immunomodulation.

Bacterial biofilm formation protects bacteria from antibiotics and the immune system, excessive inflammation further complicates treatment. Here, iron-based metal-organic framework (MIL-101)-loaded riboflavin nanoparticles are designed for the therapeutic challenge of biofilm infection and hyperinflammation in bacterial keratitis. Specifically, MIL-101 produces a thermal effect under exogenous near-infrared light irradiation, which synergizes with ferroptosis-like bacterial death induced by iron ions to exert an effective biofilm infection eradication effect. On the other hand, the disintegration of MIL-101 sustains the release of riboflavin, which inhibits the pro-inflammatory response of macrophage over-activation by modulating their phenotypic switch. In addition, to solve the problems of short residence time, poor permeability, and low bioavailability of corneal medication, the MR@MN microneedle patch is further prepared by loading nanoparticles into SilMA hydrogel, which ultimately achieves painless, transepithelial, and highly efficient drug delivery. In vivo and ex vivo experiments demonstrate the effectiveness of this approach in eliminating bacterial infection and promoting corneal healing. Therefore, the MRMN patch, acting as an ocular drug delivery system with the ability of rapid corneal healing, promises a cost-effective solution for the treatment of bacterial keratitis, which may also lead to a new approach for treating bacterial keratitis in clinics.

Rolled-up gastroretentive oral dosages for controlled release of riboflavin and propranolol

We present a simple approach to make gelatin gastroretentive oral dosages for controlled release of riboflavin and propranolol. The approach is based on rolling-up of thin self-adhesive gelatin films, on which the reservoirs of the drugs are formed by casting. The radial position of the reservoir and, hence, the lag time and the rate of the drugs release are determined by the lateral position of the reservoir on the film before rolling. Gelatin is stabilized against dissolving in the gastric environment by enzymatic cross-linking mediated by transglutaminase (Tgase). The dosages, which have the form of tight scrolls, are stabilized against unrolling by the crosslinking of the consecutive layers. The scrolls swell in the release media, Fasted State Simulated Gastric Fluid (FaSSGF), to the dimensions which make difficult their passage by the human pyloric sphincter, but remain mechanically robust to resist the stomach peristaltic contractions during at least 24 h, sufficient for the drug release. Eventually the scrolls are degraded by the release media and can be evacuated from the stomach.

Riboflavin-loaded soy protein isolate cold gel treated with combination of high intensity ultrasound and high hydrostatic pressure: Gel structure, physicochemical properties and gastrointestinal digestion fate

Transglutaminase (TGase) was added to soy protein isolate (SPI) dispersion after the combination treatment of high intensity ultrasound (HIU) and high hydrostatic pressure (HHP) to catalyze the formation of cold gel, which was used to encapsulate riboflavin. The structure, physicochemical properties and in vitro digestion characteristics of riboflavin-loaded SPI cold gel were investigated. HIU-HHP combined treatment enhanced the strength, water retention, elastic property, thermal stability and protein denaturation degree of riboflavin-loaded SPI cold gels, and improved the gel network structure, resulting in a higher encapsulation efficiency of riboflavin and its chemical stability under heat and light treatment. HIU-HHP combined treatment reduced the erosion and swelling of SPI cold gel in simulated gastrointestinal fluid, and improved the sustained release effect of SPI gel on riboflavin by changing the digestion mode and rate of gel. In addition, HIU-HHP combined treated gels promoted the directional release of riboflavin in the simulated intestinal fluid, thereby improving its bioaccessibility, which was related to the secondary structure orderliness, tertiary conformation tightness and aggregation degree of protein during the gastrointestinal digestion. Therefore, HIU-HHP combined treatment technology had potential application value in improving the protection, sustained/controlled release and delivery of SPI cold gels for sensitive bioactive compounds.

Inclusion of konjac glucomannan in pea protein hydrogels improved the rheological and in vitro release properties of the composite hydrogels

In this study, a composite hydrogel consisting of pea protein and konjac glucomannan (KG) was fabricated using three approaches, namely neutral, salt-set, and alkaline gelation. Hydrogels made from pea protein were brittle and weak. The addition of KG improved the elasticity and water holding capacity of the pea protein hydrogels. Concomitantly, a decrease in syneresis rate and swelling of the composite hydrogels was observed. The alkaline-set hydrogels exhibited the highest resilience to strain. Thixotropicity was found to be less pronounced for salt-set hydrogels. Sulphate had a greater positive effect on the structural recovery and negative effect on hysteresis area than chloride due to the greater salting-out effect of the sulphates. The addition of KG facilitated the formation of an interconnected structure with limited mobility of biopolymer chains. A sharp increase in G' and G" during the temperature ramp indicated the predominance of hydrophobic interactions towards the aggregation of biopolymers. The infrared spectra of the hydrogels revealed a change in secondary structure of proteins on addition of KG. A controlled in vitro release of riboflavin was observed in neutral and salt-set hydrogels. The alkaline-set hydrogels exhibited a prolonged gastric retention time, thereby establishing in vitro antacid activity in the gastric environment.

Host-Guest Encapsulation of RIBO with TSC4X: Synthesis, Characterization, and Its Application by Physicochemical and Computational Investigations.

In our present work, we synthesized a new encapsulated complex denoted as RIBO-TSC4X, which was derived from an important vitamin riboflavin (RIBO) and p-sulfonatothiacalix[4]arene(TSC4X). The synthesized complex RIBO-TSC4X was then characterized by utilizing several spectroscopic techniques such as 1H-NMR, FT-IR, PXRD, SEM, and TGA. Job's plot has been employed to show the encapsulation of RIBO (guest) with TSC4X (host) having a 1:1 molar ratio. The molecular association constant of the complex entity (RIBO-TSC4X) was found to be 3116.29 ± 0.17 M-1, suggesting the formation of a stable complex. The augment in aqueous solubility of the RIBO-TSC4X complex compared to pure RIBO was investigated by UV-vis spectroscopy, and it was viewed that the newly synthesized complex has almost 30 times enhanced solubility over pure RIBO. The enhancement of thermal stability upto 440 °C for the RIBO-TSC4X complex was examined by TG analysis. This research also forecasts RIBO's release behavior in the presence of CT-DNA, and at the same time, BSA binding study was also carried out. The synthesized RIBO-TSC4X complex exhibited comparatively better free radical scavenging activity, thereby minimizing oxidative injury of the cell as evident from a series of antioxidant and anti-lipid peroxidation assay. Furthermore, the RIBO-TSC4X complex showed peroxidase-like biomimetic activity, which is very useful for several enzyme catalyst reactions.

Rational design cold-set interpenetrating network hydrogel based on wheat bran arabinoxylans and pea protein isolates for regulating the release of riboflavin in simulated digestion

Cold-set interpenetrating polymer network gels as riboflavin (RF) delivery vehicles based on wheat bran arabinoxylans (AX) and pea protein isolate (PPI) were developed via enzymatic-crosslinking. The impact of AX concentrations on the physicochemical property, in vitro digestion property and microstructure of IPN gels was explored. Increased concentrations of AX enhanced the viscoelasticity of IPN gels and resulted in a more compact microstructure. However, at a concentration of 5.0 % (w/v), the faster and stronger crosslinking of AX molecules caused separate network gel between PPI and AX. The IPN gel improved the encapsulation efficiency and release property of embedded RF as compared to PPI gel. SEM results showed that IPN gel maintained a complete network structure after gastric digestion. Particularly, the IPN gel with 1.0 % AX exhibited a homogeneous and complete network structure even after intestinal digestion, which explained the reason for the highest encapsulation efficiency and lowest release ratios of RF.

Chitosan/Lactic Acid Systems: Liquid Crystalline Behavior, Rheological Properties, and Riboflavin Release In Vitro.

Chitosan or its derivatives exhibit lyotropic liquid crystalline mesophases under certain conditions due to its semi-rigid structures. This work describes the development of chitosan-based biocompatible systems that include new components: lactic acid and non-ionic surfactants. Polarized optical microscopy studies revealed that these systems are capable of forming gels or lyotropic liquid crystals (LLCs) in a certain range of chitosan and lactic acid concentrations. According to the viscosity studies, the rheological flow of the LLCs can be accurately described by the Casson flow model. The intermolecular interactions of the LLC components were studied by FTIR spectroscopy. According to the FTIR data, hydrogen bonding is supposed to be responsible for the formation of the LLCs. In the studied systems, this LLC complex exists as the [ChitH+·CH3-CH(OH)-COO-] ion pair. The studied gel and LLCs were shown to possess the most prolonged release capabilities for riboflavin among similar binary LLC systems. The supramolecular organization and rheological characteristics of the studied chitosan-based systems were found to affect the release of riboflavin.

Cold-set oat protein isolate--gellan gum binary gels with various microstructures: Fabrication, characterization, formation mechanism, and controlled release properties

Cold-set oat protein isolate (OPI) and gellan gum (GG) binary gels with various microstructures was fabricated with different amounts glucono-δ-lactone (GDL) as a carrier to deliver bioactive compounds. OPI and GG formed gel-like mixture at pH 8 mainly via hydrophobic interactions with a binding constant (Ka) of 3.85 × 104 M−1, while the mixture showed highest storage modulus (G′) with 0.1% GG (w/w, based on the dry weight of protein). Novel binary gels fabricated with 7% OPI (w/v) and 0.1% GG (w/w) (OGG) were then developed through addition of various amounts of GDL. For the gels prepared with 1.5% and 4.0% GDL (w/w, based on the dry weight of protein, pH 7.1 and 6.3, respectively), heterogeneous network formed by co-aggregated OPI and GG with a small amount of small GG particles dispersed within large aqueous pores were fabricated mainly through hydrophobic interactions. For OGG-3 (9.0% GDL, w/w) and OGG-4 (20.0% GDL, w/w), the final pH was 5.1 and 4.1 that around or lower than OPI's isoelectric point, hydrogen bonding and electrostatic attractive forces were developed between two polymers since OPI contained neat or positive charges, resulting in a compact coupled OPI-GG network structure. Heterogeneous network with small GG particles dispersed in pores allowed OGG-2 (4.0% GDL, w/w) had comparable good gel hardness to the gel formed with 9.0% GDL (w/w, OGG-3) with coupled microstructure, whereas OGG-3 showed a better ability to restrict the swelling induced release of riboflavin in SGF, and allowed sustainable release of riboflavin in SIF. Therefore, OPI-GG binary gels with various microstructures prepared by simple acidification method at ambient temperature have great potential to be used as delivery systems for heat-sensitive bioactive compounds in food and non-food applications.

Influence of ultrasound and enzymatic cross-linking on freeze-thaw stability and release properties of whey protein isolate hydrogel.

This study investigated the effect of ultrasound and enzymatic cross-linking on the freeze-thaw (FT) stability and release properties of whey protein isolate hydrogels. We evaluated the FT stability by the changes in the microstructure, riboflavin retention, syneresis, water holding capacity (WHC), and texture of gels subjected to 3 FT cycles. High-intensity ultrasound (HUS) and transglutaminase (TGase)-mediated cross-linking improved the FT stability of whey protein isolate hydrogels loaded with riboflavin (WPISAR), as demonstrated by a more uniform and denser porous structure, significantly higher riboflavin retention, WHC, and textural properties, and lower syneresis after 3 FT cycles than those of untreated hydrogels. Furthermore, HUS- and TGase-mediated cross-linking decreased protein erosion and swelling ratio of WPISAR in simulated gastrointestinal fluids (SGIF) and reduced the riboflavin release rate in SGIF both with and without the addition of digestive enzymes. After 3 FT cycles, faster riboflavin release occurred due to a more porous structure induced by ice crystal formation compared with their unfrozen counterparts as detected by confocal laser scanning microscopy. High-intensity ultrasound- and TGase-mediated cross-linking alleviated the FT-induced faster riboflavin release rate in SGIF. High-intensity ultrasound- and TGase-treated gel samples showed that both diffusion and network erosion were responsible for riboflavin release regardless of FT. These results suggest that HUS- and TGase-mediated cross-linking improved the FT stability of WPISAR with a high riboflavin retention, and might be a good candidate as a controlled-release vehicle for riboflavin delivery to overcome undesired FT processing.

Studies of Encapsulation Efficiency and Swelling Behavior of Riboflavin Encapsulated by Alginate and Chitosan

Vitamin B2, also known as riboflavin, has an important function to human tissue development and energy metabolism. Riboflavin is sensitive and unstable to external influences such as light and reducing agents. In addition, most of the absorption of nutrients occurs in the intestines so the release of riboflavin in gastric need to be avoided. To overcome this problem, riboflavin was microencapsulated with sodium alginate and chitosan polymer using ionic gelation emulsification method. The purpose of this research was to study the microencapsulation itself, and also to study the effect of the riboflavin composition used upon the encapsulation efficiency and the effect of pH medium on microcapsules swelling behavior. In this research, microcapsule characterization was also carried out using FTIR and SEM. The FTIR result shows that there are crosslinking which indicated by functional groups changes between the samples during the formation of microcapsules. The SEM result show that the dried microcapsule has rougher and wrinkled surface. In efficiency encapsulation study, microencapsulation was carried out by varying the composition of riboflavin, that is 50, 75, 100, 125, and 150 mg. Optimal encapsulation efficiency was obtained at 125 mg with an efficiency encapsulation of 51.56%. The microcapsules sample were also tested for their swelling ability by putting the microcapsules into the solution with various pH ranging from 1.2, 2.5, 4.1, 5.2, 6.8 and 8. The swelling test results showed that the characteristics of microcapsules were suitable for the human digestive system because the higher the pH of the medium, then the higher the microcapsules swelling ability.

Preparation and properties of riboflavin-loaded sanxan microcapsules

Sanxan is a novel microbial polysaccharide approved by the Chinese government for use in the food industry. In this study, we evaluated the usability of sanxan as a drug carrier using riboflavin as a model drug. Sanxan microcapsules were prepared using an extrusion dripping method. The microcapsules were loaded with riboflavin and the release effect was evaluated by in vitro digestion. Microcapsules with a sphericity factor greater than 0.95 and regular appearance could be obtained when the concentration of sanxan was 0.4% and 0.06 M HCl was used as the fixing solution; the hardness of the microcapsules was 633.3 g and their elasticity was 0.44. In the simulated gastric fluid, 3.3% of riboflavin was released, while in the simulated intestinal fluid, this value was 47.4%; this happened when the loading ratio of riboflavin to sanxan in the microcapsules was 7 g g−1. Scanning electron microscopy showed that the viscosity structure of sanxan was an important factor for the slow release of riboflavin. These results revealed that sanxan can be used as a new polysaccharide material for oral delivery.

Characterization of alginate extracted from Sargassum latifolium and its use in Chlorella vulgaris growth promotion and riboflavin drug delivery

Alginates derived from macroalgae have been widely used in a variety of applications due to their stability, biodegradability and biocompatibility. Alginate was extracted from Egyptian Sargassum latifolium thallus yielding 17.5% w/w. The chemical composition of S. latifolium is rich in total sugars (41.08%) and uronic acids (47.4%); while, proteins, lipids and sulfates contents are 4.61, 1.13 and 0.09%, respectively. NMR, FTIR and TGA analyses were also performed. Crystallinity index (0.334) indicates alginate semicrystalline nature. Sodium alginate hydrolysate was evaluated as Chlorella vulgaris growth promoter. The highest stimulation (0.7 g/L biomass) was achieved by using 0.3 g/L alginate hydrolysate supplementation. The highest total soluble proteins and total carbohydrates were 179.22 mg/g dry wt and 620.33 mg/g dry wt, respectively. The highest total phenolics content (27.697 mg/g dry wt.), guaiacol peroxidase activity (2.899 µmol min−1 g−1) were recorded also to 0.3 g/L alginate hydrolysate supplementation. Riboflavin-entrapped barium alginate-Arabic gum polymeric matrix (beads) was formulated to achieve 89.15% optimum drug entrapment efficiency (EE%). All formulations exhibited prolonged riboflavin release over 120 min in simulated gastric fluid, followed Higuchi model (R2 = 0.962–0.887) and Korsmeyer–Peppas model with Fickian release (n ranges from 0.204 to 0.3885).

Examining the effect of bovine serum albumin on the properties and drug release behavior of β-lactoglobulin-derived amyloid fibril-based hydrogels

Herein, we report the use of β-lactoglobulin (β-LG) combined with bovine serum albumin (BSA) for the preparation of amyloid-based hydrogels with aim of delivering riboflavin. The incorporation of BSA enhanced β-LG fibrillogenesis and protected β-LG fibrils from losing fibrillar structure due to the pH shift. The mechanical properties of hydrogels were observed to be positively correlated with the number of amyloid fibrils. While the addition of BSA induced amyloid fibril formation, its presence between the fibril chains interfered with the entanglement of fibril chains, thus adversely affecting the hydrogels' mechanical properties. Hydrogels' surface microstructure became more compact as the number of amyloid fibrils rose and the presence of BSA could improve hydrogels' surface homogeneity. In vitro riboflavin (RF) release rate was found to be correlated with the number of fibrils and BSA-RF binding affinity. However, when the digestive enzymes were present, the influence of BSA-RF affinity was alleviated due to enzymes' destructive and/or degradative effects on BSA and/or hydrogels, thus the release rate relied on the number of fibrils, which could be adjusted by the amount of BSA. Results indicate that the additional component, BSA, plays an important role in modulating the properties and functions of β-LG fibril-based hydrogels.

Riboflavin Biosynthesis and Overproduction by a Derivative of the Human Gut Commensal Bifidobacterium longum subsp. infantis ATCC 15697

Riboflavin or vitamin B2 is the precursor of the essential coenzymes flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). Despite increased interest in microbial synthesis of this water-soluble vitamin, the metabolic pathway for riboflavin biosynthesis has been characterized in just a handful of bacteria. Here, comparative genome analysis identified the genes involved in the de novo biosynthetic pathway of riboflavin in certain bifidobacterial species, including the human gut commensal Bifidobacterium longum subsp. infantis (B. infantis) ATCC 15697. Using comparative genomics and phylogenomic analysis, we investigated the evolutionary acquisition route of the riboflavin biosynthesis or rib gene cluster in Bifidobacterium and the distribution of riboflavin biosynthesis-associated genes across the genus. Using B. infantis ATCC 15697 as model organism for this pathway, we isolated spontaneous riboflavin overproducers, which had lost transcriptional regulation of the genes required for riboflavin biosynthesis. Among them, one mutant was shown to allow riboflavin release into the medium to a concentration of 60.8 ng mL–1. This mutant increased vitamin B2 concentration in a fecal fermentation system, thus providing promising data for application of this isolate as a functional food ingredient.

Controlled release of riboflavin encapsulated in pea protein microparticles prepared by emulsion-enzymatic gelation process

Riboflavin was encapsulated in pea protein microparticles crosslinked by transglutaminase and the release properties of this system was studied in simulated gastric (pH = 1.2) and intestinal (pH = 7.4) fluids. The microparticles were obtained from a water-in-oil emulsion and subsequent enzymatic gelation. In presence of polyglycerol polyricinoleate as hydrophobic surfactant, magnetic agitation was preferred to high-speed homogenization in order to form spherical microparticles of ~150 μm average size. The encapsulation efficiency of crystallized riboflavin (RF) varied from 74% to 84% depending on the amount of loaded RF (0.1–1.0 wt%). According to the kinetic power law model, the release mechanisms of riboflavin under simulated gastrointestinal conditions were governed by diffusion in absence of digestive enzymes and by a support degradation phenomenon in their presence. The designed microparticles represented a potential system for the delivery of riboflavin up to the intestinal digestion phase.

Corn fiber gum-soybean protein isolate double network hydrogel as oral delivery vehicles for thermosensitive bioactive compounds

Corn fiber gum (CFG)-soybean protein isolate (SPI) double network (DN) hydrogels, with varied concentrations of CFG, were fabricated at room temperature in order to develop a biocompatible vehicle for the oral administration of thermosensitive bioactive compounds. Riboflavin (vitamin B2) was encapsulated in the gel as a model bioactive compound. The compound release properties of these DN hydrogels were investigated in simulated gastric and simulated intestinal fluids (SGF and SIF). DN hydrogels with 0.25% of CFG were found to be the most desirable of those assayed for the oral administration of bioactive compounds. The results of low-field nuclear magnetic resonance (LF-NMR) relaxometry measurement showed that the pH-responsive DN hydrogels exhibited low swelling ratio in the SGF but larger swelling ratio in the SIF. The results of degree of proteolysis and scanning electron microscopy (SEM) showed that significant release of riboflavin from DN hydrogels in the SIF was due to large swelling, CFG matrix erosion and the proteolysis of SPI. The release mechanisms of riboflavin through the DN hydrogels in the simulated intestinal fluid followed non-Fickian diffusion based on Peppas' semi-empirical equation.

Bioinspired pH-sensitive riboflavin controlled-release alkaline hydrogels based on blue crab chitosan: Study of the effect of polymer characteristics

Recently, the application of natural biocompatible polymeric hydrogels for the conception of drug delivery matrices has attracted widespread interest. Thus, in the present study, riboflavin pH-sensitive drug delivery hydrogels were developed based on blue crab chitosan (Cs), via direct dissolution in alkali/urea aqueous solution at low temperatures. First, the effect of Cs characteristics in terms of acetylation degree (AD) and molecular weight (Mw) on the structural, mechanical, thermal, swelling and in vitro biodegradation of Cs-based hydrogels were studied. Data from overall analysis revealed that Cs with low AD and high Mw exhibited improved mechanical properties, as evidenced by the compressive and rheological behaviors tests, thermal resistance, swelling behavior and in vitro degradation kinetics. However, hydrogels pore sizes were reduced with the AD decrease and Mw increase. Additionally, hydrogels in PBS (pH 5.5) underwent quicker degradation, compared to those immersed in PBS (pH 7.4). In the drug delivery model, the kinetics of Riboflavin release, through the Cs-based hydrogels were monitored. The Riboflavin release exhibited typical deliverance patterns, with significantly higher released amounts in more acidic systems. Therefore, drug encapsulation within the conceived pH-sensitive Cs-based hydrogels could provide suitable and promoting microenvironment for drugs delivery.

Water compatible molecularly imprinted polymer for controlled release of riboflavin as drug delivery system

In this study, facile and green method for synthesis of imprinted material was introduced which subsequently applied for efficient delivery of Riboflavin (vitamin B2). Characterization of the prepared polymer was carried out by the Fourier Transform Infrared (FT-IR) spectroscopy and Scanning Electron Microscopy (SEM). The weight ratio of functional monomer to template over 5–20 W/W% was evaluated in order to find the best loading condition and subsequent release into synthetic biomimetic media namely simulated body fluid (SBF) and media including 1% NaCl solution, simulated gastric fluid (SGF) solution at pH 1.5 and the solution of 1% W/W Na2SO4. Under optimized condition, the highest value of released riboflavin was achieved at 1:20 ratio of riboflavin to polymer in NaCl (55.07%) media which providing that the body requirement about 5 days of this vitamin between minimum effective concentration (MEC) and maximum safe concentration (MSC) of Riboflavin in short release time (45 min). Moreover, the experimental data were fitted to Higuchi model in order to calculate Higuchi solubility constant.