Release Of Fatty Acids Research Articles

Deficiency of Adipose Triglyceride Lipase Induces Metabolic Syndrome and Cardiomyopathy in Zebrafish

Lipid metabolism dysfunction is related to clinical disorders including obesity, cancer, liver steatosis, and cardiomyopathy. Impaired lipolytic enzymes result in altered release of free fatty acids. The dramatic change in dyslipidemia is important in lipotoxic cardiomyopathy. Adipose triglyceride lipase (ATGL) catalyzes the lipolysis of triacylglycerol to reduce intramyocardial triglyceride levels in the heart and improve myocardial function. We examined the role of ATGL in metabolic cardiomyopathy by developing an Atgl knockout (ALKO) zebrafish model of metabolic cardiomyopathy disease by continuously expressing CRISPR/Cas9 protein and atgl gene guide RNAs (gRNAs). The expressed Cas9 protein bound to four gRNAs targeting the atgl gene locus, facilitating systemic gene KO. Ablation of Atgl interfered with lipid metabolism, which induced hyperlipidemia and hyperglycemia. ALKO adults and embryos displayed hypertrophic hearts. ALKO presented a typical dilated cardiomyopathy profile with a remarkable reduction in four sarcomere genes (myosin heavy chain 7-like, actin alpha cardiac muscle 1b, myosin binding protein C3, and troponin T type 2a) and two Ca2+ handling regulator genes (tropomyosin 4b and ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2b). Immune cell infiltration in cardiac tissue of ALKO provided direct evidence of advanced metabolic cardiomyopathy. The presently described model could become a powerful tool to clarify the underlying mechanism between metabolic disorders and cardiomyopathies.

Statin-Induced Geranylgeranyl Pyrophosphate Depletion Promotes PCSK9–Dependent Adipose Insulin Resistance

Statin treatment is accepted to prevent adverse cardiovascular events. However, statin therapy has been reported to be dose-dependently associated with increased risk for new-onset type 2 diabetes mellitus (T2DM). Proprotein convertase subtilisin/kexin type 9 (PCSK9) is expressed in adipose tissue and is positively correlated with lipid metabolism. It is, however, unknown if PCSK9 participates in adipocyte insulin resistance occurring as a result of statin use. Our goal was to use an in vitro adipose tissue explant approach to support the hypothesis that PCSK9 regulates statin-induced new-onset T2DM. Studies were performed using Pcsk-/- and C57Bl/6J control mice. Pcsk9-/- and control mice were fed a high-fat diet to affect a state of chronically altered lipid metabolism and increased PCSK9. Epididymal fat was excised and incubated with atorvastatin (1 µmol/L) in the absence and presence of insulin or geranylgeranyl pyrophosphate (GGPP). PCSK9 mRNA was evaluated using quantitative rtPCR. We further examined the effects of atorvastatin on insulin-mediated AKT signaling in adipose tissue explants by immunoblotting. Atorvastatin was found to upregulate PCSK9 gene expression in adipose tissue. The metabolic intermediate GGPP is required to downregulate PCSK9 expression. PCSK9 deficiency protects against statin-induced impairments in insulin signaling. Moreover, supplementation with GGPP reversed atorvastatin-induced suppression of insulin signaling. Furthermore, the basal and atorvastatin-stimulated release of free fatty acids was observed in adipose tissue from wild-type mice but not PCSK9 deficient mice. Collectively, we describe a novel mechanism for PCSK9 expression in adipose tissue that could mediate statin-impaired adipose insulin resistance.

Effects of coating layers chitosan/pectin on lipid stability and in vitro digestion of astaxanthin-loaded multilayer emulsions

Astaxanthin (Ax), a hydrophobic carotenoid from marine animals and microalgae, is claimed to exhibit various beneficial biological activities. In this study, quillaja saponin, chitosan and pectin were employed to formulate Ax-loaded primary, secondary, and tertiary multilayer emulsions using a layer-by-layer electrostatic deposition technique. The effects of the coating layers on the antioxidation of lipid, in vitro digestion properties, and Ax bioaccessibility were investigated. The results showed that the coating layers improved the lipid stability during the storage process and gastrointestinal digestion of lipid droplets effectively. In particular, the release of free fatty acids was reduced by nearly 20% in tertiary multilayer emulsions coated by chitosan and pectin. Meanwhile, the coating layers postponed the release of Ax effectively. The multilayer emulsions developed in this study could be used to load, protect, and deliver astaxanthin, which may contribute to the development of more effective functional foods, supplements, and pharmaceutical products.

Encapsulation of fish oil by complex coacervation and freeze drying with modified starch aid

This work developed a combination method between gelatin-sodium hexametaphosphate (SHMP) complex coacervation and freeze-drying with aid of starch sodium octenyl succinate (SSOS) for the preparation of fish oil-encapsulated powders. The results suggested the preparation and properties of the powders were dependent on the used gelatins. Porcine skin gelatin (PSG), bovine skin gelatin (BSG), and fish gelatin (FG) had good gelling properties and could successfully prepare the complex coacervates and powders. The solution pH and SHMP:gelatin mass ratio were critical parameters for the preparation of the complex coacervates. SSOS was a good freeze-drying aid to increase the encapsulation efficiency (EE). The obtained fish oil-encapsulated powders had similar fish oil loading capacity values (24.0–26.2%) and different fish oil EE values: PSG (98.4%) > FG (96.4%) > BSG (95.2%). After the in vitro digestion process, the fish oil-encapsulated powders were digested and free fatty acid release percentage was dependent on gelatin source: PSG (55.5%) > FG (34.1%) > BSG (27.0%). This work provided useful information for the potential use of gelatin, fish oil, and SSOS in the development of powder foods.

Effect of phospholipid matrix on emulsion stability, microstructure, proteolysis, and in vitro digestibility in model infant formula emulsion

The effects of adding different phospholipid (PL) matrices [milk sphingomyelin (SM) vs soy phosphatidylcholine (PC)] on emulsion stability, microstructure, and in vitro simulated lipid digestion were examined using a Model Infant Formula Emulsion (MIFE). The emulsion stability of MIFE increased significantly with PL addition (0.1 and 0.2 %). Compared to sole MIFE or MIFE + PC, the incorporation of SM resulted in increased emulsion stability (p < 0.05) and a greater amount of free fatty acid release (p < 0.05) during in vitro simulated digestion. This was mainly due to the reduction of intensive droplet aggregation, thus providing a large surface area and improved digestibility. This is further experimentally supported by the evolution of particle size distribution, zeta-potential, and microstructure analysis using confocal laser scanning microscopy. The incorporation of SM in the emulsion formation significantly delayed digestion of β-lactoglobulin during in vitro digestion. Lipid digestibility in MIFE was altered depending on the type of PL matrix, and SM displayed a superior effect to PC. Thus, the creation of a novel emulsion interface by the appropriate selection of emulsifiers can be used to improve lipid digestion in infants and obtain desirable nutritional consequences.

Interaction between whey protein and soy lecithin and its influence on physicochemical properties and in vitro digestibility of emulsion: A consideration for mimicking milk fat globule

In this study, from the perspective of simulating the milk fat globule (MFG) emulsion, the interaction between soybean lecithin (SL) and the main protein in milk, whey protein (WP), and its effect on physical characteristics and lipid digestion were investigated through multiple spectroscopic techniques and in vitro digestion. The mechanism of SL and WP was static quenching, indicating that a complex formed between WP and SL through hydrophobic interaction and hydrogen bonding. The addition of SL changed the secondary structure of WP. When the ratio of SL to WP was 1:3, the obtained SL–WP emulsion that simulated milk fat globule exhibited the smallest particle size distribution and the highest absolute value of zeta potential. In addition, the emulsion exhibited high encapsulation efficiency (91.67 ± 1.24 %) and good stability. Compared with commercially available infant formula (IF), the final free fatty acid release of prepared SL–WP emulsion was close to that of human milk (HM). The addition of lecithin increased the digestibility of fat and the release of free fatty acids, and the digestive characteristic and particle size change also were closer to that of HM from results of kinetics of free fatty acid release and microstructure analysis.

The interfacial destabilization of bile salt-emulsified oil droplets, essential for lipase function, is mediated by Lycium barbarum L. leaf polysaccharides

A bile salt (BS)-stabilized water/oil interface is a prerequisite for the pancreas lipase (PL) mediated hydrolyzation of triacylglycerol. Previous fat digestion research mainly focused on direct lipase inhibition by bioactive substances while ignoring the destructive role of these substances on BS-stabilized lipid emulsions. Here, we showed that polysaccharides (LP) weakly suppressed PL but destroyed BS-stabilized emulsions and thus inhibited free fatty acid (FFA) release. Physicochemical analysis showed that LP actions toward emulsion stability were mainly due to endogenous minerals (28%) in LP which affected aggregation and amphiphilic properties of BS-polysaccharide composites. This was evidenced by high wettability and low interfacial tension in demineralized polysaccharide and BS composites, with three phase contact angles which were identified as 80.4° and 95.6° as well as interfacial tensions as 9.26 and 9.37 mN/m, respectively. However, the low wettability, which was detected as 76.2° and 78.6° as well as high interfacial tension as 10.77 and 11.02 mN/m, respectively, was only observed in mineral-containing polysaccharides and BS composites. Further investigations showed that interactions with mineral-containing polysaccharides occupied hydrophobic sites in BS thereby elevating composite hydrophilicity. These findings provided novel insights into the effects of plant-sourced polysaccharides on fat digestion from an interface chemistry perspective, and in particular, they deepened our understanding of polysaccharide interactions with intestine-secreted lipid digestants.

Protective effect of hierarchical emulsions stabilised by oil/water droplets in lutein delivery

SummaryIn this study, lutein was encapsulated in a hierarchical emulsion (HE) stabilised by oil/water (O/W) droplets. The particle size and ζ‐potential of the HE did not considerably change after thermal treatment, and the retention rate of lutein (76.56%) was higher than that of bulk oil after 21 days of storage. The addition of lutein was found to inhibit lipid oxidation under both light and dark conditions. The results of in‐vitro digestion suggested that the free fatty acid release and bioaccessibility (32.51%) of the HE were higher than those of bulk oil. This study indicated that the HE prepared from soyasaponin‐stabilised O/W droplets as emulsifiers has application potential in the encapsulation of hydrophobic food functional factors; furthermore, this study provides delivery strategies for supplements and drugs.

Walnut peptide loaded proliposomes with hydroxyapatite as a carrier: Fabrication, environmental stability, and in vitro digestion attribute

To explore the feasibility of hydroxyapatite (HAP) as the carrier for proliposomes and improve the stability of walnut peptides (WPs), WPs-loaded proliposomes (WPs-PROLIPs) with hydroxyapatite (HAP) as the carrier were fabricated, and the physicochemical properties, environmental stability as well as in vitro simulated digestion release performance of the proliposomes were investigated. The proliposomes with HAP possessed smaller particle sizes and higher encapsulation efficiencies than those without HAP. FTIR analysis revealed that hydrogen bonds formed between HAP and phospholipids in the proliposomes. The inclusion of HAP in WPs-PROLIPs led to the improvement of the thermal degradation stability and environmental stabilities of the system. HAP also induced the conversion of free water into bound water in the proliposomes, as evaluated by LF NMR. In addition, proliposomal encapsulation did not affect the antioxidant activity of WPs-PROLIPs and the lateral order of the liposome membrane. Finally, in vitro digestion showed that the addition of HAP endowed the proliposomes with a retarded free fatty acid release effect, which was dependent on the weight ratio of phospholipids to HAP. These results offer opportunities for the use of HAP as a feasible carrier and lyoprotectant for proliposomes encapsulating biopeptides.

ODP150 Hypertriglyceridemic Induced Acute Pancreatitis with Diabetic Ketoacidosis Treated with Insulin: A Case Report

Abstract Introduction Acute pancreatitis due to hypertriglyceridemia with DKA is a unique entity. The diagnosis of acute pancreatitis in DKA is challenging as most patients present with abdominal pain, and elevated amylase, and lipase are quite common in DKA. Hypertriglyceridemia-induced pancreatitis (HTGP) accounts for 1 to 35 percent of all cases of acute pancreatitis. Pancreatitis may occur in DKA with high anion gap metabolic acidosis. Lack of insulin results in lipolysis with release of free fatty acids. Increased free fatty acids presented to the liver, causes increased VLDL, which inhibits lipoprotein lipase in peripheral tissues and results in elevated triglycerides. Case presentation A 29-year-old morbidly obese male with Asperger's syndrome, prediabetes, and hyperlipidemia presented with a 3-day history of sharp, non-radiating, and progressively worsening right upper abdominal pain, associated with nausea, non-bilious vomiting, and shortness of breath. His past medical history was notable for a prior bout of gallstone pancreatitis, for which he underwent a laparoscopic cholecystectomy. On his arrival in the emergency department, he was afebrile, but tachycardic (HR 139), mildly hypertensive (BP 149/98) with a Kussmaul breathing saturating 98% on room air. His lung sounds were clear but diminished and his abdomen was soft but diffusely tender, without peritoneal signs. Initial laboratory tests showed a neutrophilic leukocytosis of 12K, glucose of 867 mg/dL, hyponatremia (corrected 131), ketonuria, with elevated lipase 1,164 U/L. Venous blood gas showed a pH of 7.18 and PCO2 24 mmHg. He also had severe hypertriglyceridemia with a total triglyceride level of &amp;gt; 9,000 mg/dL. His CT abdomen showed peripancreatic inflammation consistent with acute pancreatitis. He was admitted to the ICU, and initially managed with aggressive intravenous fluids and pain control, then was started on an insulin drip for diabetic ketoacidosis and hypertriglyceridemia. His hospital course was notable for the development of acute kidney injury requiring hemodialysis. His triglyceride level gradually decreased and was 396 U/L on hospital day #9. He was discharged on fenofibrate, Lantus 20 units daily and insulin lispro 10 units with meals. Conclusion Hypertriglyceridemia is an uncommon but a well-established cause of acute pancreatitis leading to significant morbidity and mortality. In this case, a prediabetic patient presented with DKA and further investigation revealed HTGP. There is currently a paucity of data to support an optimal treatment for this condition; however, both insulin and plasmapheresis have been successfully employed. Plasmapheresis helps remove plasma lipoproteins and reduces triglyceride more rapidly than insulin and has been recommended for severe cases. In our case, insulin infusion alone significantly reduced the triglyceride level. Presentation: No date and time listed

LBSUN87 Human Cidec Transgene Improves Lipid Metabolism And Protects Against High Fat-diet Induced Glucose Intolerance In Mice

Abstract CIDEC expression in adipose tissue positively correlates with insulin sensitivity in obese humans. Further, a single-nucleotide CIDEC variant (E186X) causes lipodystrophy, hypertriglyceridemia, and insulin resistance. To establish the unknown mechanistic link between CIDEC and maintenance of systemic glucose homeostasis, we generated transgenic mouse models expressing CIDEC (Ad-CIDECtg) and CIDEC E186X variant (Ad-CIDECmut) transgene specifically in the adipose tissue. Ad-CIDECtg but not Ad-CIDECmut mice were protected against high-fat diet (HFD)-induced glucose intolerance. Transcriptomics and lipidomics revealed the role of CIDEC in lipid metabolism. Serum triglycerides, cholesterol, and low-density lipoproteins were lower in HFD-fed Ad-CIDECtg mice compared to their littermate controls. Mechanistically, CIDEC regulates the enzymatic activity of ATGL via interacting with its activator, CGI-58, to reduce free fatty acid (FFA) release and lipotoxicity. We confirmed that CIDEC is indeed a vital regulator of lipolysis in adipose tissue of obese humans. Finally, treatment with recombinant CIDEC decreased triglyceride breakdown in visceral human adipose tissue. Our study unravels a central pathway whereby adipocyte-specific CIDEC plays a pivotal role in regulating adipose lipid-metabolism and whole-body glucose homeostasis. In sum, our findings identify human CIDEC as a potential 'drug' or a 'druggable' target to reverse obesity-induced lipotoxicity and glucose intolerance. Presentation: Sunday, June 12, 2022 12:30 p.m. - 2:30 p.m.

Docosahexaenoic Acid Counteracts the Hypoxic-Induced Inflammatory and Metabolic Alterations in 3T3-L1 Adipocytes.

Hypoxia is caused by the excessive expansion of the white adipose tissue (AT) and is associated with obesity-related conditions such as insulin resistance, inflammation, and oxidative stress. Docosahexaenoic acid (DHA) is an omega-3 fatty acid reported to have beneficial health effects. However, the effects of DHA in AT against hypoxia-induced immune-metabolic perturbations in adipocytes exposed to low O2 tension are not well known. Consequently, this study aimed to evaluate the impact of DHA on markers of inflammation, metabolism, apoptosis, and oxidative stress in 3T3-L1 cell adipocytes exposed to low O2 tension (1% O2) induced hypoxia. The apoptosis and reactive oxygen species (ROS) rates were evaluated. Metabolic parameters such as lactate, FFA, glycerol release, glucose uptake, and ATP content were assessed by a fluorometer. The expression of HIF-1, GLUT1 and the secretion of adipocytokines such as leptin, adiponectin, and pro-inflammatory markers was evaluated. DHA-treated hypoxic cells showed significantly decreased basal free fatty acid release, lactate production, and enhanced glucose consumption. In addition, DHA-treatment of hypoxic cells caused a significant reduction in the apoptosis rate and ROS production with decreased lipid peroxidation. Moreover, DHA-treatment of hypoxic cells caused a decreased secretion of pro-inflammatory markers (IL-6, MCP-1) and leptin and increased adiponectin secretion compared with hypoxic cells. Furthermore, DHA-treatment of hypoxic cells caused significant reductions in the expression of genes related to hypoxia (HIF-1, HIF-2), anaerobic metabolism (GLUT1 and Ldha), ATP production (ANT2), and fat metabolism (FASN and PPARY). This study suggests that DHA can exert potential anti-obesity effects by reducing the secretion of inflammatory adipokines, oxidative stress, lipolysis, and apoptosis.

English

Sweat is important in thermoregulation, helping to dissipate heat in the body by evaporation in hot conditions in dairy cows. The purpose of this study was to evaluate the variation of sweat fatty acid compositions in summer conditions in Jersey and Holstein dairy cattle for their potential thermoregulatory roles in animal health protection. Temperature, relative humidity and Temperature-Humidity Index (THI) values were calculated for the intensity of stress in the indoor environment. The chemical compounds of sweat were determined by gas chromatography device and inter-breeds differences were revealed. As a result of analyzes, a total of 23 fatty acids were detected in both breeds. It was understood that there were 15 fatty acids in the Jersey breed. Palmitic acid was detected at a rate of 27.72 % in Holstein cows and 25.16 % in jersey cows that may be due to a higher rate of fatty acid release from muscle tissue, so that it may be less resistant than the jersey breed in heat stress conditions. It can be said that palmitic acid can be an indicator for heat stress conditions. ∑SFA and ∑PUFA contents of the sweat of Holstein cows were lower than those of Jersey breeds while ∑MUFA content was higher than those of Jersey breeds. The high expulsion of ∑SFA and ∑PUFA in the sweat of the Jersey cows can provide the function of delaying the body's dehydration by forming a hydrolipid film on the skin surface in hot conditions

Insight of rheology, water distribution and in vitro digestive behavior of starch based-emulsion gel: Impact of potato starch concentration

Potato starch-based emulsion gels were fabricated that consisted of whey protein-coated oil droplets dispersed in a gelatinized starch matrix. The impact of potato starch concentration (0–16%) on the rheological properties, water distribution, and in vitro digestive behavior of the emulsion gels was examined. Potato starch-based emulsion gels exhibited shear-thinning and viscoelastic properties. The apparent viscosity increased with progressing starch concentration, and a stronger gel network formed with massive starch. In low-field 1H nuclear magnetic resonance, the transverse relaxation time significantly reduced with progressing starch matrix, indicating a tighter bond between the water molecules with others. The gastrointestinal tract was used to monitor the in vitro digestive behavior of the starch-based emulsion gels in each phase (oral, stomach, and small intestine). Increasing particle size and a reduction charge were found in emulsion gel with potato starch. And the gel network structure remained in emulsion gel with 8–16% potato starch. Overall, a slower rate of initial free fatty acid release was determined in the emulsion system containing starch, and a greater extent of lipid digestion occurred in the hard gel. The results of this study may facilitate the development of starch-based emulsion gels for utilization in foods, cosmetics, and other semi-solid commercial products.

Effect of Monosodium Glutamate on the Digestibility of Different Nutrients Using Standardized Static In vitro Digestion Model

Monosodium glutamate (MSG) is a flavor enhancer and food additive with a unique umami taste. Due to its widespread use in humans, this study focused on the cytotoxicity, anti-diabetic effect, and interaction with protein digestion by performing a standardized static in vitro digestion model and lipid digestion by estimating free fatty acids released from 0.5 g of olive oil during intestinal lipolysis. The study showed that monosodium glutamate has an apparent cytotoxic effect on the Caco-2 cell line in a dose-dependent manner. MSG glutamate also showed low inhibitory activity on alpha-glucosidase enzyme even at high concentrations (16.3 % at 1800 ppm). By performing simulated in vitro digestion to study the interaction between MSG and protein digestion, followed by MTT study, total protein determination, and pH drop method, all results concluded that MSG affected proteolysis. Finally, the impact of MSG on lipolysis was studied through a free fatty acid release test. The results of the study demonstrated that MSG harmed fat digestibility in a concentration-dependent manner. As a result, it is essential to conduct further studies, especially in vivo studies, to determine the potential negative effects of MSG on human health.

Preparation, characterization and digestive mechanism of plant-derived oil bodies-based oleogels structured by chitosan and vanillin

This study has explored a facile approach to structure oleogels using plant-derived oil bodies (OBs) as oil droplets that were electrostatically coated by chitosan as an outer layer, followed by vanillin induced cross-linking of the coated droplets. The electrostatic interaction, Schiff base reaction and hydrogen bond cross-linkages were the main driving forces between the OBs, chitosan and vanillin for the construction of oleogels, as shown by the ζ-potential, FTIR and fluorescence spectroscopy results. Morphological analysis demonstrated that chitosan coating on the OBs prevented the oiling-off of oleogels, while vanillin addition resulted in closely packed 3D-network structures. From differential scanning calorimetry analysis, it was exhibited that the peak temperature and enthalpy of oleogels increased as the chitosan or vanillin concentration increased. Macro-properties showed that the cross-linked oleogels were thermally stable with excellent thixotropic recovery ability (97.6%), as well as, had higher gel strength (G′ > 18,000 Pa) and gel hardness (13.10 N) values, suggesting that interfacially adsorbed chitosan and interconnected vanillin networks together contributed to the development of solid oleogel structures. Moreover, lipid digestion kinetics revealed that cross-linking slowed down the free fatty acids release rate of oleogels, which may have been due to: i) compact coating layer formed by chitosan partially restricted the interfacial displacement by enzymes, ii) integrated networks induced by vanillin reduced the void spaces available for the diffusion of lipases and bile salts, which were confirmed by microstructural digesta. This study provides a novel strategy for the production of edible oleogels using OBs to potentially replace solid fats.

Pickering emulsion stabilised by double‐modified starch particles and its delivery property for curcumin

SummaryRice starch was hydrophobised by octenyl succinic anhydride (OSA) and then modified by roast dry heating at 180°C for 40 min (RDH‐40) to improve its digestibility. The oil‐in‐water Pickering emulsions stabilised by double‐modified starch particles were fabricated. The emulsion stability under different formulation parameters was investigated. Results showed that the suitable parameters for fabrication of Pickering emulsion were RDH‐40 concentration of 4.0 wt.%, oil volume ratio of 50% and pH of the system was 7.0. The addition of NaCl and sucrose (0–200 mmol/L) slightly reduced the emulsion stability. Microstructure results revealed that the starch particles and a few gelatinized starch macromolecules distributed around the oil droplets, making the emulsion more stable. Compared with the emulsions stabilised by Tween 20 and OSA starch, the Pickering emulsion stabilised by 4.0% RDH‐40 starch showed the highest loading and embedding rates for curcumin at 25 and 40°C. The emulsion stabilised by RDH‐40 had a faster free fatty acid release rate and higher curcumin bioaccessibility than that stabilised by OSA starch during in vitro digestion. This study helps to develop new starch‐based Pickering emulsions for the delivery of oil‐soluble bioactive substances.

A comprehensive study on physicochemical properties, bioactive compounds, and emulsified lipid digestion characteristics of Idesia polycarpa var. Vestita Diels fruits oil

Idesia polycarpa var. vestita Diels fruits oil (IPO) has the potential to broaden the availability of healthy vegetable oil and relieve pressure on the edible oil supply. In this study, we compared the physicochemical, bioactivity, and digestive properties of IPO, olive oil (OO), and soybean oil (SO) to comprehensively evaluate the edible potential of IPO. The results revealed no significant differences in relative density or refractive index among the three oils. IPO was rich in β-sitosterol (366.74 mg/100 g), β-tocopherol (8.42 mg/100 g), and α-tocopherol (37.10 mg/100 g). The digestive properties of IPO emulsion were investigated for the first time using in vitro simulated digestion. The IPO emulsion stood out regarding its free fatty acid release (88.03 %). Finally, the IPO emulsion released mainly unsaturated fatty acids and had a higher monoacylglycerol content. This study provides new insights into IPO as a high-quality edible vegetable oil.

Cardiometabolic Syndrome : An Emerging Global Health Issue

Cardio metabolic syndrome (CMS) is a combination of metabolic dysfunctions characterized by insulin resistance, impaired glucose tolerance, dyslipidemia, hypertension and central obesity. CMS is recognized as a disease entity by the World Health Organization (WHO) and American Society of Endocrinology. CMS represents a constellation of metabolic abnormalities that are risk factors for cardiovascular diseases These cardiovascular and metabolic derangements individually and interdependently lead to increases in cerebrovascular disease(CVD) morbidity and mortality, making the CMS an established and strong risk factor for premature CVD. Data found that the prevalence of cardiometabolic syndrome (CMS) is increasing in all age groups, both in men and women from the latest National Health and Nutritional Examination Survey (NHANES).Alteration in fatty acid metabolism, such as excessive fatty acid release in the plasma is likely to contribute to these metabolic abnormalities. There is an established and proven benefits in reversing abnormal responses and decreasing cardiovascular risks by the treatment strategies including moderate physical activity, weight reduction, rigorous blood pressure control, correction of dyslipidemia and glycaemic control. J Bangladesh Coll Phys Surg 2022; 40: 287-291

Pressurized hot water extraction, structural properties, biological effects, and in vitro microbial fermentation characteristics of sweet tea polysaccharide

Although sweet tea is rich in bioactive polysaccharides, the knowledge regarding their structures, bioactivities, and gut microbial metabolism is still limited. Therefore, in order to promote the application of sweet tea polysaccharide (STP) in the food industry, the pressurized hot water extraction (PHWE) of STP was optimized, and its structural properties and biological effects as well as microbial fermentation characteristics were investigated. The maximum extraction yield (4.64 % ± 0.03 %) of STP extracted by PHWE was obtained under the optimal conditions. Both homogalacturonan and arabinogalactan might exist as major polysaccharide fragments in STP. Additionally, STP exerted obviously in vitro antioxidant, anti-diabetic, and immunostimulatory effects, which might be related to its chemical properties, such as uronic acids, conjugated polyphenolics, and esterification degree. Furthermore, STP could be consumed by intestinal microbiota, and its fermentability was about 54 % at the end stage of fecal fermentation. Indeed, STP could modulate the microbial composition via improving the growth of several beneficial microbes, causing the release of beneficial short-chain fatty acids. Collectively, the findings indicate that the PHWE is an efficient method for extracting bioactive polysaccharides from sweet tea, and results can also provide a scientific basis for developing STP into functional foods or functional ingredients.