Release Bolus Research Articles

The difference between two strains of Ostertagia ostertagi in resistance to morantel tartrate

Borgsteede F. H. M. 1988. The difference between two strains of Ostertagia oslertagi in resistance to morantel tartrate. International Journal for Parasitology 18: 499–502. A comparison was made of the efficacy of morantel tartrate against a susceptible laboratory strain and a field strain of Ostertagia ostertagi isolated during the use of a morantel-sustained release bolus. Twenty calves were divided into four groups. Calves of groups 1 and 2 were infected with the susceptible strain and calves of groups 3 and 4 were infected with the other strain. Calves of groups 2 and 4 were then treated after 28 days with 10 mg kg −1 morantel tartrate and slaughtered on day 34. Faecal egg output of calves of group 2 fell to zero after treatment, while calves of group 4 remained positive, though lower than before. After morantel treatment pepsinogen values dropped markedly in calves of group 2, but less in calves of group 4. Worm numbers of calves of group 2 were reduced by 98.5% compared with those of group 1. Calves of group 4 showed a 77.4% reduction in worm burden compared with calves of group 3. It was concluded that it is possible to select an O. ostertagi strain with morantel resistance by the use of a morantel-sustained release bolus.

Effects of ammoniumiron-hexacyanoferrate on the accumulation of radiocesium in reindeer

<span style="font-size: xx-small;"><p align="justify">It is concluded that a sustained release bolus for administration of a cesium binder of the ironhexa-cyanoferrate type provides a promising method for reduction of radiocesium uptake from contaminated pastures.</p><p align="justify">Included is also a discussion after Hove's lecture.</p></span>

Feed intake of grazing calves exposed to trichostrongyle infection and treated with the morantel sustained release bolus

Herbage intake was measured in two groups of 20 first-year grazing cattle. The animals in one group each received a morantel sustained release bolus at turnout to control nematode parasitism and the animals in the other group remained untreated. The latter group showed a mean peak faecal egg count of 655 eggs per gram (e.p.g.) in October associated with high serum pepsinogen concentration and clinical signs of ostertagiasis, compared with a peak of 119 e.p.g. in the treated group which remained in good health. In September the daily voluntary feed intake of the untreated animals was significantly depressed (94 g kg −1 body weight vs. 77 g kg −1 P < 0.001), but no difference in digestive efficiency was recorded between the two groups. This difference in feed intake was associated with a 47 kg mean live weight advantage of the treated animals at housing.

Anthelmintics and control

Anthelmintic control of Ostertagia ostertagi in cattle presents some special problems because the arrested larval stage (hypobiotic EL 4) tolerates all of the older anthelmintics. The only anthelmintics on the North American market that are effective against this stage as well as the adult and developing stages are ivermectin and fenbendazole. In addition to these, the newer broad-spectrum benzimidazoles and probenzimidazoles, albendazole, febantel, netobimin and oxfendazole, are effective and available in other countries. These anthelmintics can be used for prophylaxis of Type II ostertagiasis. The older anthelmintics, levamisole, morantel tartrate, thiabendazole, coumaphos, haloxon and phenothiazine, are effective against the adults and to some extent the developing stages of O. ostertagi so that they can be used to treat Type I ostertagiasis. They can also be used to prevent incoming larvae from establishing if they are administered continuously over a long period of time. This is possible with long term in-feed administration or by the use of the morantel slow release bolus. In cooler temperate regions, where cattle are housed over the winter, this bolus is given at turnout to remove any parasites in the animals and to kill incoming larvae for 60–90 days. This can prevent the build up of significant infective larvae on pasture so that very few arrested L 4 larvae accumulate in the summer/autumn, effectively preventing Type II ostertagiasis from occurring later. The use of ivermectin and the newer benzimidazoles in intermittent or slow release devices should prove highly effective in the control of Type I and II ostertagiasis, as well as of subclinical ostertagiasis. To achieve maximum economic benefit, the use of anthelmintics should be based on sound epidemiological considerations, so that stock are not rapidly reinfected after treatment.

Use of an oxfendazole pulse release bolus in calves exposed to natural subclinical infection with gastrointestinal nematodes.

During the first grazing season a group of calves treated with an oxfendazole pulse release bolus achieved a mean (+/- sem) weight gain of 140.7 (+/- 6.7) kg compared with 106.5 (+/- 5.7) kg by a group treated once with ivermectin mid-season, and 116.9 (+/- 6.9) kg by a group which received no treatment. This economic advantage was maintained during the period of winter housing. By the end of the second grazing season, during which the animals received no anthelmintic medication, they weighed on average 20 kg more than the wholly untreated group, a difference which was not statistically significant. No signs of clinical disease were observed in either the animals dosed with a pulse release bolus or the undosed control animals during the two year trial period. The treatment with the oxfendazole pulse release bolus greatly reduced the degree of pasture contamination in the first year but in the second year those animals that had been treated in the first year developed higher worm egg counts (P less than 0.001) and thus augmented the levels of pasture contamination compared with the untreated control animals. Nematodirus battus and N filicollis both produced low grade but fertile infestations in the calves.

'Front-loaded' oxfendazole pulse release bolus for the suppression of parasitism in grazing calves: a field study.

'Front-loaded' oxfendazole pulse release bolus for the suppression of parasitism in grazing calves: a field study.

Assessment of an oxfendazole pulsed release bolus for control of parasitic gastroenteritis in calves in a rotational grazing system

A group of 71 Friesian bullocks, aged six to nine months, vaccinated against lungworm, were randomly allocated on a liveweight basis to two groups of 40 and 31 animals. At turn-out each calf in the group of 40 calves was dosed orally with a pulsed release bolus designed to deliver five doses of oxfendazole at regular intervals during a period of up to 130 days, the first dose being released about 21 days after administration. The group treated with the bolus grazed 2.4 ha and the control group grazed 3.6 ha of permanent pasture for six weeks before having additional access to similar areas of silage aftermath. The control group was treated 99 days after turn-out and when they were housed with fenbendazole (7.5 mg/kg). Faecal worm egg counts, plasma pepsinogen activities, pasture larval counts and liveweights were recorded fortnightly. Significant reductions in worm egg counts and plasma pepsinogen activities were recorded in the calves dosed with the pulsed release bolus together with significant improvements in the liveweight of younger calves compared with control animals. Pasture larval counts were lower in the fields grazed by animals treated with the bolus.

Use of an oxfendazole pulse release bolus in the control of parasitic gastroenteritis and parasitic bronchitis in first-season grazing calves

The efficacy of the oxfendazole pulse release bolus system for the control of parasitic gastroenteritis and parasitic bronchitis in first-season grazing calves was evaluated in Belgium. Twenty-two calves were allocated to two groups. The calves in one group received a bolus at the time of turn out, while the other group remained untreated. The efficacy of the bolus was assessed by comparison of faecal worm egg counts, plasma pepsinogen concentrations, the antibody response to Ostertagia, Cooperia and Dictyocaulus species total plasma protein and albumin concentrations, and weight gains throughout the grazing season and the housing period. The oxfendazole pulse release bolus provided good control of parasitic gastroenteritis dominated by ostertagia. The effects of parasitic gastritis were greatly reduced as shown by the significantly lower values of serum pepsinogen and ostertagia antibody titres. The use of the bolus further reduced the adverse effects of parasitism as indicated by better liveweight gains and normal total plasma protein and albumin concentrations whereas in the untreated control group hypoproteinaemia and hypoalbuminaemia were observed. Most animals exhibited clinical signs of parasitic bronchitis at the end of the grazing season, and the bolus may not adequately control parasitic bronchitis in all cases at all times.

Interactions between chemoprophylaxis and immunity to bovine parasitic gastroenteritis and bronchitis: pilot studies using an oxfendazole pulse release bolus

In separate experiments, the immune status of six matched pairs of yearling heifers from a field trial in which both parasitic gastroenteritis and husk had occurred in control animals, was tested with a single massive challenge of either Dictyocaulus viviparus or Ostertagia ostertagi. The clinical responses of untreated controls and animals that had carried an oxfendazole pulse release intraruminal device (OPRB) were in all cases similar (with the exception of one lung-worm-challenged control that succumbed to a fulminating pulmonary hypersensitivity reaction), indicating that both groups possessed comparable degrees of protection. Faecal larval/egg-output data and serum gastrin levels, however, suggested that larger worm populations had established in the OPRB cattle.

Control of parasitic gastroenteritis in calves with the morantel sustained release bolus: effect of prior grazing with adult cattle.

Control of parasitic gastroenteritis in calves with the morantel sustained release bolus: effect of prior grazing with adult cattle.

Control of gastrointestinal parasitism in calves in Sweden over six years using the morantel sustained release bolus

During the 6 years 1979–1984, the use of the morantel sustained release bolus (MSRB) was monitored in first year grazing calves. Twenty-four to 30 calves each year were allotted to two groups (controls and MSRB-treated) and turned out at the end of May on the same pasture divided into two equal areas. After housing in October, each group was kept in boxes and fed concentrates, whey and hay. The calves were weighed at monthly intervals until the first animals were sent to slaughter. No cases of clinical ostertagiasis Type I were observed in the control calves during the grazing seasons, but in one year (1983) clinical signs occured 2 weeks after housing. The number of overwintering larvae was influenced by pasture contamination the previous season and climatic conditions during winter and spring. The fecal egg output of the control animals during the grazing season did not reflect the level of pasture contamination at turnout. The build-up of pasture larval contamination during the later part of the grazing season was influenced by the climatic conditions. The fecal egg output of the treated calves was low during the entire grazing season resulting in a significantly reduced pasture contamination. A significantly reduced live-weight gain in the control calves was demonstrated at housing in five of the six years. When heavily infected at housing, the performance of the control calves was still influenced negatively during the fattening period indoors.

Intraruminal soluble glass boluses containing melatonin can induce early onset of ovarian activity in ewes.

Soluble phosphate glass boluses (preparations A, B, and C) have been developed to release melatonin into the reticulo-rumen of ewes at relatively different (fast, medium or slow) rates. A fourth type (preparation D) containing no melatonin was used as a sham control. Four groups of seasonally anoestrous Suffolk-cross ewes were respectively dosed with preparations A, B, C or D on 4 July. Plasma samples were collected twice weekly for melatonin and progesterone assay. In Groups A, B and C, elevated daytime plasma concentrations of melatonin could be detected for about 5 weeks after bolus administration. However, the pattern of hormone release was variable between groups, with Group C animals maintaining higher plasma melatonin concentrations for a longer period. The control animals had undetectable daytime melatonin levels. The onset of cyclic ovarian activity in the animals treated with the 'slow' release bolus (Group C) was significantly (P less than 0.05) advanced compared to the control group. The 'fast' and 'medium' release treatments (Groups A and B) did not significantly alter the onset of ovarian activity. The results indicate the potential of a novel and convenient method of melatonin delivery for induction of early breeding activity in ewes.

Pathophysiological and parasitological studies on Cooperia oncophora infections in calves

Calves which had received daily infections of Cooperia oncophora over a six week period showed inappetence, weight loss, impaired nitrogen retention and a loss of plasma proteins into the gut. During this period the infections occurred in the duodenum, jejunum and ileum and many larval stages were present in the mucosa of the small intestine. At necropsy, on weeks 3, 6 and 10 after the first infection, stunting and thickening of the villi were noted with excessive mucus production. Prior administration of a morantel slow release bolus prevented these changes. By week 12 most of the worms had been expelled yet a loss of plasma proteins to the gut was still occurring.

Evaluation of the morantel sustained release bolus in cows and calves.

The efficacy of the morantel sustained release bolus (MSRB) in controlling gastrointestinal parasitism in beef cattle was assessed during the 1982 spring-autumn grazing season. Forty-eight cows and their calves were allotted to three equal groups. One group (T-1) served as a nonmedicated control group. One MSRB was administered to each calf of the T-2 group, and to each cow and calf of the T-3 group at the beginning of the study. The efficacy of the bolus was assessed by comparison of weight gain performance and parasitological data (fecal worm egg counts, herbage larval counts, worm counts from tracer and principal trial calves, and plasma pepsinogen level determinations). Though not statistically significant, treated calves from Group T-2 had a numerical mean weight gain advantage of 2.6 kg, and those from Group T-3 of 4.7 kg, over control calves. Average daily gains (ADG) for the three groups of calves were 0.69, 0.72, and 0.73 kg, respectively. Untreated cows from Group T-2 and treated cows from Group T-3 outperformed the control cows by 12.3 and 7.5 kg, respectively. Fecal worm egg counts from both groups of treated calves were significantly (P less than 0.01) lower than counts from control calves during the entire 169-day trial; notably, egg counts were reduced by 99% 28 days after MSRB administration to both groups of calves. There were no significant differences in the number of eggs counted from the three groups of cows, probably because of the very low numbers of eggs encountered. Mean total worm burdens of principal calves (six per group) necropsied at trial termination indicated a 91% (P less than 0.01) reduction in Group T-2 and an 87% reduction (P less than 0.01) in Group T-3. Worm-free tracer calves were introduced onto pastures every 28 days to monitor availability of infective larvae. The mean number of worms recovered at necropsy from tracer calves that grazed with control cattle increased as the season progressed. However, the numbers of parasites recovered each month from mid-August through mid-October from tracers that grazed pastures with treated cattle were lower (P less than 0.05) than those levels displayed at trial initiation. In addition, the mean numbers of worms from treated group tracers were lower than from the controls for each necropsy period.(ABSTRACT TRUNCATED AT 400 WORDS)

Efficacy of morantel sustained release bolus against gastrointestinal nematodes in first season grazing Holstein calves

The efficacy of the morantel sustained release bolus (MSRB) in reducing gastrointestinal parasitism in first season grazing calves was evaluated during the summer-autumn grazing seasons of 1982 and 1983 in western Oregon. Each of 38 calves (1982) and 40 calves (1983) were randomly assigned to either control or treatment groups which were given MSRB on the day of turnout onto pasture. Mean worm burdens from tracer calves grazed with treated animals in 1982 and 1983 showed overall reductions of 86.4% ( P > 0.05) and 84.3% ( P < 0.01), respectively, compared to tracers grazed with controls. Ostertagia ostertagi, Cooperia oncophora and Nematodirus helvetianus were the primary nematodes collected at necropsy. Twelve full-season 1982 tracer animals (6 treated and 6 control) indicated an 88.1% ( P < 0.05) overall reduction in mean worm burdens. Mean fecal worm egg per gram (EPG) counts of treated animals reflected a reduction of 69% ( P < 0.05) in 1982 and 90% ( P < 0.05) in 1983. Autumn inhibition of O. ostertagi was observed. In the 1982 trial the control animals showed a slight mean weight gain advantage over the treated group from Day 84 until Day 160 (trial termination) when the mean difference was 7.9 kg. The final mean weight gain advantage of treated animals in 1983 was 13.5 kg ( P < 0.05). These trials demonstrated that the MSRB was an effective anthelmintic for reducing gastrointestinal parasitism in grazing calves and for decreasing pasture larval contamination.

Phophylaxis of nematode infections in cattle with an indwelling rumino-reticular ivermectin sustained release bolus

Ivermectin delivered continuously from a rumino-reticular sustained release device was prophylactically effective in preventing establishment of nine nematode parasite species in multiply-exposed cattle. Ivermectin dosages which permitted ⩽1% of infected control calf worm populations to establish ranged from <2.5 μg kg −1 day −1, which totally prevented infection with Dictyocaulus viviparus and Oesophagostomum radiatum, to ≅30 μg kg −1 day −1 required to suppress Nematodirus helvetianus to the same extent. Between these extremes, in decreasing order of sensitivity to enterical sustained release ivermectin, were Ostertagia ostertagi, Trichostrongylus axei, Haemonchus placei, Copoperia punctata, C. oncophora and T. colubriformis which were maximally affected at ⩽10 μg kg −1 day −1 of ivermectin.

The effect upon the intake of grazing calves of infection with the gastrointestinal nematode ostertagia ostertagi

Parasitic gastroenteritis (PGE), largely attributable to the abomasal parasite Ostertagia ostertagi is a cause of major economic loss in the cattle industry. It has been suggested from studies on infected housed animals, together with pair fed controls, that the characteristic loss of weight occurring as a consequence of PGE is due mainly to loss of appetite, (Dargie, 1980). Thus in the present study the intention was to measure the effect of parasitism on intake under natural grazing conditions.2 groups of 20 Freisian steer calves (175 kg at turnout), were grazed from May to September 1984 on adjacent 5 acre paddocks which had been contaminated the previous autumn by cattle Infected with 0. ostertagi. The allocation of calves to each group was such that the mean weight and range of weights within each group were similar. At turnout each calf in group A received a (Morantel slow release bolus), group B being untreated controls. The calves were weighed and sampled regularly for faecal egg counts.

Production, parasitological and carcase evaluation studies in steers exposed to trichostrongyle infection and treated with a morantel bolus or fenbendazole in two consecutive grazing seasons

Friesian steers were exposed to natural trichostrongyle infection and left untreated (controls) or treated with either a morantel sustained release bolus or fenbendazole at two week intervals (clean) in each of two consecutive grazing seasons. In the second grazing season the mean liveweight gains of the clean, morantel treated and control groups of cattle over 152 days were 105, 131 and 109 kg respectively (morantel treated greater than clean, control P less than 0.02). The cattle were slaughtered following an indoor fattening period. The dressed carcase weight, killing out percentage and related carcase measurements were superior in the treated groups. Eighty per cent of the clean and morantel treated cattle graded satisfactorily and qualified for subsidy payment whereas less than 30 per cent of the control cattle were successful. Rib joint analyses showed significantly lower total weight, eye muscle weight and area together with a higher bone content in control cattle.

Metabolism and growth in housed calves given a morantel sustained release bolus and exposed to natural trichostrongyle infection

Three groups of calves, previously grazed on permanent pastures contaminated with bovine trichostrongyle (Ostertagia and Cooperia species) infective larvae, were housed from October to May. During the grazing season one group had received fenbendazole at fortnightly intervals to suppress trichostrongyle infections, one received a morantel sustained release bolus before grazing to limit trichostrongyle contamination of the pasture, and the control group was only medicated when heavy infections caused clinical type 1 ostertagiasis. Digestibility of the whole diet was poorer in the control cattle, particularly for dry matter, crude protein and energy fractions. Balance studies conducted throughout the winter housed period showed that both increased faecal and urinary nitrogen outputs in the control animals contributed to a significantly reduced overall nitrogen retention. The effects were most apparent during clinical type 2 ostertagiasis, which occurred in March in the control group.

Efficacy of Morantel Tartrate in a Sustained Release Bolus on the Control of Subclinical Gastrointestinal Parasitism in First-Year Grazing Dairy Replacements

Thirty-four heifers (24 Holstein, 8 Ayrshire, 2 Jersey) and 12 Holstein steer calves were grouped into 23 blocks of 2 animals each according to breed, sex, and body weight. Animals in each block were randomly assigned to control or treated groups. The treated group received an anthelmintic bolus containing morantel tartrate before they were permitted access to pasture. A 2.6ha pasture was divided in half with a double fence; the resulting two pastures had separate feed and water troughs. Steer calves were slaughtered upon termination of the trial to ascertain gastrointestinal parasite burdens. Two worm-free tracer calves were placed on each pasture every 2 mo and slaughtered after 4 wk of grazing to determine parasite infectivity of pastures. Herbage samples were obtained monthly and analyzed for infective larvae. The trial was conducted for 141 d during the grazing season. Treatment resulted in 90% reduction in infective larvae on pasture, 74% reduction in fecal worm-eggs, 91% reduction in adult worm burdens, and a trend toward reduced worm burdens in tracer calves by 52%. An overall average daily gain of .68 and .88kg was obtained for control and treated animals. Reproductive data for the heifers remaining in the herd from the trial showed that treatment resulted in 44 less days to first breeding.