The present study entitled “Effect of relative water content and water use efficiency of Lactuca sativa under different methods of growing”was conducted to demonstrate water saving techniques and their role in plant growth and sustainable development that given a new dimension to modern agriculture. For experiment, two varities of lettuce was used i.e., Romaine and Grand rapid. The experiment was carried out in hydroponics having growing media and also in pots with equal amount of Farm Yard Manure at the experimental house of the Division of Plant Physiology, SKUAST-J. Growing media was therefore, applied in different ratio i.e., coco peat (100 %), Coco peat + Vermicompost (1:1), coco peat + perlite (1:1), coco peat + vermiculite (1:1), coco peat + perlite + vermiculite (1:1:1), coco peat + perlite + vermiculite + vermicompost (1:1:1:1) as well as with conventional method (soil + Manure). The performance of both varieties under hydroponic treatments was compared with the plants grown by conventional method. The experimental results revealed that among both the varities, treatment T5 performed better as compared to other treatments and should be recommended to other hydroponic growers and farmers in future.