The extreme temperatures and humidity are quite disturbing climatic conditions and clothing is the only protective material in these cases. During the interactions of the human body, clothing, and the environment, to maintain thermal equilibrium, thermo physiological comfort is crucially important. In general, the thermo physiological comfort features of a fabric include thermal conductivity, thermal flow, thermal absorbtivity, thermal resistance, thickness, water vapor permeability and air permeability. In this study, 21 different kinds of woven shirt fabrics produced from fibers in various blends have been treated with both classical and nano chemicals in four different concentrations to produce fabrics having different water-oil repellent and wrinkle resistant characteristics. The effects of the fiber type, treatment method, chemical type, and chemical concentration on the thermal, water vapor and air permeability properties of the fabrics treated with various finishing processes were investigated by statistical analysis. The results of thermal related properties, air permeability and water vapor permeability of the fabrics confirmed that using cotton, lyocell, and viscose shirt fabrics treated with transfer method could be advantageous, especially in hot climatic regions.