Relative Size Research Articles

Bergmann’s rule in global terrestrial vertebrates

Abstract To date, whether Bergmann’s rule, initially developed for individual species or closely related taxa, can be applied to broader taxa remains elusive. Using global distribution data for birds, mammals, reptiles, and amphibians, both species- and assemblage-based, we examined the role of species richness and related climate variables in shaping body size patterns across the globe and the Northern Hemisphere only and whether the temperature–body size relationship had a phylogenetic signal. The results show that when species in all four classes were combined, body size increased with latitude but declined with temperature, consistent with Bergmann’s rule. Each group showed somewhat unique patterns but was mostly consistent with Bergmann’s rule. The body size–temperature relationships were not constrained by phylogenetic relatedness. Warmer places had both large and many small-bodied species, whereas cold places had proportionally more large species. The temperature-dependent variations in body size have important implications for macroecology under climate change.

HCPrune: A Hierarchical Classification Pruning Method Based on Receptive Field Size

Abstract Model pruning, as an optimisation algorithm for convolutional neural network (CNN) models, can effectively compress and accelerate various complex CNN models. Recent network pruning algorithms usually focus on removing unimportant and redundant convolutional kernels or channels in the network. This paper propose a hierarchical pruning algorithm, HCPrune, which classifies and then prunes the convolutional layers of a CNN model to improve the model prediction accuracy while reducing the number of parameters and FLOPs. In this paper, the CNN convolutional layers are divided in terms of the size relationship between the receptive field of each layer of the network and the resolution of the input image, and use the VGG and Resnet series of networks based on some popular pruning methods to conduct experiments on the CIFAR10 and CIFAR100 datasets and ensure that the same number of parameters and FLOPs compared with the basic pruning methods. The VGG16 network improves the accuracy by 0.42% in CIFAR10 and 0.47% in CIFAR100, and the ResNet164 improves the accuracy by about 0.2% in the same configuration.

Biological Characteristics of the Glacier Lantern Fish Benthosema glaciale (Myctophidae) in Icelandic Waters andthe Irminger Sea During Summer.

Myctophids are one of the most diverse and abundant fish families in the mesopelagic zone, making them a key component in the marine pelagic community. In the North Atlantic, Benthosema glaciale (glacier lantern fish) is considered the most abundant myctophid north of 35° N, yet some regions within its extensive range lack information on its basic biological parameters. We investigated the growth and described the distribution of B. glaciale in Icelandic waters, including the southern continental shelf, Iceland Basin, and Irminger Sea. Length distribution, growth, relative body condition, age, and otolith-fish size relationships were analyzed from samples collected opportunistically with pelagic trawls during the International Ecosystem Summer Survey in the Nordic Seas (IESSNS) in July 2020. In total, 1374 individuals were caught, and only a subset of 225 were measured fresh at sea and 89 were frozen and dissected on land. Total lengths of fresh fish ranged from 38 to 85 mm, while dissected individuals ranged from 40 to 74 mm and were 2 to 6 years old. Located over the Reykjanes Ridge in the Iceland Basin region, individuals had a significantly higher mean standard length and mean age, and a lower mean body condition. We reported length-weight relationships for both fresh and frozen samples, indicating evidence that preserving of the specimens through freezing affected relationships. The von Bertalanffy growth curve was calculated along with significant otolith-fish size relationships where r 2 values ranged between 0.87 and 0.92. Our research highlights the importance of cross-regional studies and provides baseline biology for B. glaciale in Icelandic waters, specifically in the southern continental slope and Reykjanes Ridge.

Detection of nanopores with the scanning ion conductance microscopy: A simulation study

During the dielectric breakdown process of thin solid-state nanopores, the application of high voltages may cause the formation of multi-nanopores on one chip, which number and sizes are important for their applications. Here, simulations were conducted to mimic the investigation of in situ nanopore detection with scanning ion conductance microscopy (SICM). Results show that SICM can provide accurate nanopore location and relative pore size. Detection resolution is influenced by the dimensions of the applied probe and separation between the probe and membranes, which can be enhanced under large voltages or a concentration gradient.

Allometry in limb regeneration and scale-invariant patterning as the basis of normal morphogenesis from different sizes of blastemas.

Axolotl (Ambystoma mexicanum) limb regeneration begins with blastemas of various sizes, in contrast to the limb developmental process. Despite this size variation, normal limb morphology, consistent with a limb stump size, is regenerated. This outcome suggests the existence of underlying scale-invariant mechanisms. To identify such mechanisms, we examined the allometric relationships between blastema size, and Sonic Hedgehog (Shh) and Fibroblast Growth Factor 8 (Fgf8) expression patterns against limb stump size. We found that all factors showed allometric rather than isometric scaling; specifically, their relative sizes decrease with an increase in limb stump size. However, the ratio of Shh/Fgf8 signaling dominant region was nearly constant, independent of blastema/body size. Furthermore, the relative spatial patterns of cell density and proliferation activity and the relative position of first digit formation were scale-invariant in the summed Shh/Fgf8 crosstalk region. This scale-invariant nature may underlie the morphogenesis of normal limbs from different sizes of blastemas.

Comparative Characteristics of the Areas of the Auricle Sectors in Young Men with Different Head Shapes

As reported in the scientific literature, data on the correlations between the shape and size of the auricle and other anatomical structures of the human body are very limited. There are no data on the relationship between the auricle and the shape of the head. The aim of the study was to determine the sizes of the auricle sectors and their correlations with the shape of the head. Material and methods. The study involved young men (n=401) aged 18-21 years. The shape of the head was determined by cephalometry. The right auricle was studied on digital images. The authors determined the area of individual sectors and their percentage ratio and analysed a linear correlation. The dynamics of the relationship between sectors in each group and between groups was studied in accordance with the shape of the head. Results. The sizes of the auricle sectors and their percentage ratio were detected. Correlation links between the sectors, the dynamics of the relationships between the sectors within one group and between the groups by head shape were revealed. Mobile and permanent sectors and dependence of their relative sizes on the head shape were identified. The relationship between the sizes of the sectors and the head shape was demonstrated. Conclusion. The areas of the auricle sectors increase from S1 to S4 regardless of the head shape. In the dolichocephalic group, all sizes have the largest absolute values, in mesocephalics - average, in brachycephalics - the smallest. The ratio of the S1 sector area to the area of other sectors are maximum in the dolichocephalic group. The ratios of the S2/S4 and S3/S4 sectors areas do not depend on the head shape and absolute values of the parameters, and are the same in all groups.

Wet Beveling of Microinjection Needles Utilizing Constant Air Pressure for Feedback on Needle Opening.

Microinjection needles are a critical tool in the delivery of genome modification reagents, CRISPR components (guide RNAs, Cas9 protein, and donor template), and transposon system components (plasmids and transposase mRNA) into developing insect embryos. Sharp microinjection needles are particularly important during the delivery of these modifying agents since they help minimize damage to the embryo being injected, thereby increasing the survival of these embryos as compared to injection with non-beveled needles. Further, the beveling of needles produces needles that are more consistent from needle to needle as compared to needles opened by randomly breaking the needle tip by brushing the tip against an object (side of a coverslip, the surface of the embryo to be injected, etc.). The process of wet beveling of microinjection needles with constant pressure air delivered to the needle allows the person beveling the needle to know when the needle is open (presence of bubbles) and also gives a relative indication of how large a needle opening has been created. The relative opening size in the needle can be determined by adjusting the air pressure delivered to the needle until an equilibrium is reached and bubbles stop flowing from the tip of the needle. The lower the pressure at which the equilibrium is reached, the larger the needle size; and conversely, the higher the pressure, the smaller the needle size.

Pulmonary Pharmacokinetics of Antibody and Antibody Fragments Following Systemic and Local Administration in Mice.

Objective: This study aimed to investigate the effect of molecular size on the pulmonary pharmacokinetics (PK) of proteins following systemic and local administration in wild-type mice. Methods: A non-cross-reactive antibody trastuzumab, and F(ab')2, Fab, and scFv fragments of this antibody were used for the investigation. Proteins were injected intravenously or via intratracheal instillation, and PK was measured in plasma, lungs, trachea, bronchi, and bronchoalveolar lavage (BAL) using ELISA. Concentrations in BAL were urea normalized. Results: Following systemic administration, the biodistribution coefficient (BC) for lungs, trachea, bronchi, and BAL was 11%, 11%, 15%, and 2% for the antibody; 15%, 7%, 13%, and 8% for F(ab')2; 25%, 17%, 28%, and 46% for Fab; and 14%, 1%, 2%, and 50% for scFv. The antibody exposure in BAL was ~50-fold lower than plasma and ~5-7-fold lower than lung tissues. A tissue-dependent BC vs. molecular size relationship was observed, where distribution in tissues was the highest for Fab (50 kDa), and scFv demonstrated the highest distribution in the BAL. PK data generated following local administration were quite variable; however, local dosing resulted in BAL exposures that were 10-100-fold higher than those achieved after systemic dosing for all proteins. The BAL antibody concentrations were 100-1000-fold higher than plasma concentrations initially, which normalized by day 14. For most proteins, local dosing resulted in higher lung concentrations than trachea and bronchi, opposite to what was observed after systemic dosing. Conclusions: The PK data presented here provide an unprecedented quantitative insight into the effect of molecular size on the pulmonary disposition of proteins following systemic and local administration.

The effect of relative density, granularity and size of geogrid apertures on the shear strength of the soil/geogrid interface

The increasing use of geogrid in various geotechnical projects has made the evaluation of the shear behavior of soil reinforced with geogrid become particularly important. In this article, a series of large-scale direct shear tests have been performed on sand and gravel samples reinforced with geogrid. The purpose of the experiments was to investigate the impact of the geogrid mesh size and the relative density of the samples on the shear strength coefficient of the interface between soil and geogrid. In this study, 5 geogrids with different mesh sizes and one type of geotextile were used. According to the results, the average shear strength coefficient of sand and gravel samples reinforced with geogrid for different normal stresses and different relative densities was obtained between 0.72 and 0.94. As the relative density increases, the interface shear strength coefficient decreases, this means that the denser the sand, the more the shear strength of the sand/geogrid interface decreases. Based on the results, it was found that the contribution of particle interlocking in the shear resistance of the sand/geogrid interface is particularly important, so that the shear resistance coefficient of the interface increases with the increase in the size of the geogrid mesh.

When market access gains do not spur urban growth

Abstract We revisit the natural experiments of division and unification of Germany. The negative shock to local market access following the division of Germany led to a fast and strong downward adjustment of the size of West German cities near the new border. In contrast, the positive shock of reunification did not lead to any change in their relative size. Even three decades after reunification, no convergence can be observed. We show that local subsidies to East Germany could have contributed to this asymmetry in time.

Transparent, Antibiofouling Window Obtained with Surface Nanostructuring.

Biofouling is one of the key factors which limits the long-term performance of seawater sensors. Common measures to hinder biofouling include toxic paints, mechanical cleaning and UV radiation. All of these measures have various limitations. A very attractive solution would be to prevent biofilm formation by changing the surface structure of the sensor. This idea has been implemented successfully in various settings, but little work has been done on structuring optically transparent materials, which are often needed in sensor applications. In order to achieve good antibiofouling properties and efficient optical transparency, the structuring must be on the nanoscale. Here, we investigate a transparent, antibiofouling surface obtained by patterning a semihexagonal nanohole structure on borosilicate glass. The nanoholes are approximately 50 nm in diameter and 200 nm deep, and the interparticle distance is 135 nm, allowing the structure to be optically transparent. The antibiofouling properties of the surface were tested by exposing the substrates to the microalgae Phaeodactylum tricornutum for four different time intervals. This species was chosen because it is common in the Norwegian coastal waters. The tests were compared with unstructured borosilicate glass substrates. The experiments show that the nanostructured surface exhibits excellent antibiofouling properties. We attribute this effect to the relative size between the structure and the biofouling microorganism. Specifically, the small dimensions of the nanoholes, compared to the biofouling microorganism, make it more difficult for the microalgae to attach. However, lubrication of the substrates with FC-70 perfluorocarbon resulted in contamination at a rate comparable to the reference substrate, possibly due to the chemical attractiveness of the alkane chains in FC-70 for the microalgae.

A Video Dual-Domain Blind Watermarking Algorithm Based on Hadamard Transform

Addressing the compatibility challenges surrounding the robustness and reversibility of existing video watermarking techniques, this study introduces a novel video dual-domain blind watermarking algorithm leveraging the Hadamard transform. Specifically tailored for H.264 video copyright protection, the algorithm initially organizes video frames and identifies key frames for watermark embedding. Prior to embedding, the robust watermark undergoes coding preprocessing to optimize its integration. Subsequently, a 4×4 block is expanded based on the selected embedding position within the frame, followed by the application of the Hadamard transform to the enlarged block. The 1-bit robust watermark information is then embedded via the coefficient pair located in the first row of the Hadamard coefficient matrix corresponding to the expanded block. Additionally, a reversible watermark, designed to mitigate the distortions introduced during robust embedding, is generated and embedded into the remaining coefficients of the coefficient matrix using reversible embedding techniques. During watermark extraction, the dual-domain watermark can be retrieved exclusively through reversible extraction methodologies by analyzing the size relationship of coefficient pairs, eliminating the need for access to the original video data. To bolster the algorithm’s robustness, a majority-subordinate voting system is devised and implemented, effectively enhancing its resilience. Experimental findings demonstrate that, compared to similar approaches, this algorithm not only enhances the reversibility of video restoration but also exhibits superior robustness and meets the requirements for imperceptibility.

Does brain size of Asiatic toads (Bufo gargarizans) trade-off with other energetically expensive organs along altitudinal gradients?

Abstract Brain size variation is often attributed to energetic trade-offs with other metabolically expensive tissues and organs, which is a prediction of the expensive brain hypothesis (EBH). Here we examine Asiatic toads (Bufo gargarizans) along altitudinal gradients, and test size trade-offs between brain and four visceral organs (heart, liver, alimentary tract, and kidney) with altitude. Body size and scaled mass index (SMI; a proxy for total energy intake) decline with altitude, implying stronger energetic constraints at high altitudes. Relative brain size decreases along altitudinal gradients while visceral organs mostly increase in relative sizes. Using structural equation modelling, a significant negative relationship between brain size and a latent variable ‘budget’, which represents the energy allocation to the four visceral organs, is detected among high-altitudinal toads. Heart appears to have the largest and most consistent response to changes of energy allocation. No such relationships are observed among toads at middle and low altitudes, where high energy intake may allow individuals to forego energetic trade-offs. When applying EBH to poikilotherms, a great emphasis should be placed on total energy intake in addition to energy allocation. Future research on EBH will benefit from more intra-specific comparisons and the evaluation of fitness consequences beyond energy limitation.

Poetic ‘Hypertext’, Evocative ‘Figure-in-Landscape’, and the Reading of Illustrated Plays in the Late Ming

Abstract Scholars of late Ming print culture have noted a ‘scenic’ shift in book illustrations of the late Wanli period. But how did the ‘scenic’ style vary across different genres of books, and what impact did such variations have on reading? This article examines a series of drama illustrations attributed to the Hangzhou publisher Rongyu tang (RYT), and explores the RYT’s subtle reconfigurations of the relationships between figures, landscapes, and poetic captions to produce new experiences of reading plays. I argue that the RYT developed its own repertoire of ‘figures-in-landscape’ that, while intersecting with visual motifs in a wide range of paintings and prints, distinguish themselves through the fine-tuning of the relative size of figure to landscape and the integrating of poetic ‘hypertexts’. Through close examination of three figure types – traveler, fisherman, and female performer – in landscape, I show that the images gave rise to a poetically recollective, visually evocative, and culturally contextualized mode of reading that appealed to varying levels of knowledge and interests. They afforded readers the flexibility to immerse themselves in the atmosphere or emotions of select scenes, to reflect on the overarching themes of the plays, or to take an imaginative flight out of drama to appreciate the images’ broader poetic and visual associations.

Evaluating physiological responses of microalgae towards environmentally coexisting microplastics: A meta-analysis

Microplastics (MPs) are abundantly present in aquatic environments, where the phytoplankton—microalgae, are now inevitably bound to a long-term coexistence with them. While numerous studies have focused on the toxicological effects of high-concentration MPs exposure, there remains controversy over whether and how MPs affect microalgae at environmentally relevant concentrations. This study aims to draw conclusions that narrow the gap from 52 studies with varying results. Overall, MPs can inhibit growth and photosynthesis, induce oxidative damage, from which microalgae can recover after an appropriate period. Cyanobacteria exhibit greater vulnerability than chlorophyta. The relative size of MPs to algal cells potentially governs their coexistence behavior, thereby altering the mechanisms of impact. Pristine MPs may increase the production of extracellular polymeric substances (EPS) and microcystins (MCs), while aged MPs have the opposite effect. Additionally, relevant factors are systematically discussed, offering insights for future research.

Towards high-strength electrolyte-supported solid oxide fuel cells

The functionality and structural reliability of electrolyte-supported solid oxide fuel cells depend crucially upon the load-bearing capability of the ceramic electrolyte. In this work, the effect of thickness on the mechanical strength of tape-casted 8 mol% yttria-stabilized zirconia electrolyte discs was investigated. Samples with 98 % relative density and 2–3 µm grain size fabricated with thicknesses between 75 µm and 360 µm were mechanically tested using an adapted biaxial bending test. Thin electrolytes with biaxial strength up to 1 GPa could be produced. The measured characteristic strengths ranged from σ0 = 940 MPa to σ0 = 520 MPa for thin and thick samples, respectively, yielding a common Weibull modulus of m = 6. A higher strength scaled with decreasing thickness, confirming the “size effect” as in many technical ceramics. The measured ionic conductivity was above 0.1 S/cm at 1000 °C, which is in good agreement with commercial or conventionally prepared electrolytes.

Agglomeration of carbonaceous particles in a pendant water slurry droplet

Liquid spraying is used in a number of engineering applications, such as spray cooling, drying, granulation, etc., which mostly deal with colloidal solutions or slurries. Depending on the application, solid particle agglomeration can be considered a positive or negative phenomenon. Therefore, to make engineering processes reliable and stable, it is necessary take account of secondary processes affecting the size distribution of the material involved. The agglomeration of solid particles in a pendant droplet was studied experimentally. Distilled water was used as the base liquid and 60–120-μm coal particles as solid inclusions. The coal concentration in a droplet was varied in the range of 0.5–10 %. The main parameters of particle agglomeration in a droplet were recorded using the shadow method. Particle sedimentation velocities and heights of the agglomerated particle layers in a droplet were recorded as part of the experiments. These characteristics were affected by the initial liquid volume, relative particle concentration and size. Equations were obtained for the relationships determined in the experiments. These equations can help extrapolate the corresponding functions to wider variation ranges of input parameters. Recommendations have been formulated on managing the processes of slurry droplet stratification over time.

Characterization of an Advanced Blast Simulator for Investigation of Large Scale Blast Traumatic Brain Injury Studies.

Blast traumatic brain injury (bTBI) is a prominent military health concern. The pervasiveness and long-term impacts of this injury highlight the need for investigation of the physiological outcomes of bTBI. Preclinical models allow for the evaluation of behavioral and neuropathological sequelae associated with bTBI. Studies have implemented rodent models to investigate bTBI due to the relative small size and low cost; however, a large animal model with similar neuroanatomical structure to humans is essential for clinical translation. Small blast simulators are used to induce bTBI in rodents, but a large animal model demands a larger device. This study describes a large advanced blast simulator (ABS4) that is a gas-detonation-driven system consisting of 5 sections totaling 40ft in length with a cross-section of 4 × 4 ft at the test section. It is highly suitable for large animals and human surrogate investigations. This work characterized the ABS4 in preparation of large-scale bTBI testing. An array of tests were conducted with target overpressures in the test section ranging from 10 to 50 psi, and the pressure-time profiles clearly illustrate the essential characteristics of a free-field blast wave, specifically a sharp peak pressure and a defined negative phase. Multiple blast tests conducted at the same target pressure produced very similar pressure profiles, exhibiting the reproducibility of the ABS4 system. With its extensive range of pressures and substantial size, the ABS4 will permit military-relevant translational blast testing.

Multilevel approximation of Gaussian random fields: Covariance compression, estimation, and spatial prediction

The distribution of centered Gaussian random fields (GRFs) indexed by compacta such as smooth, bounded Euclidean domains or smooth, compact and orientable manifolds is determined by their covariance operators. We consider centered GRFs given as variational solutions to coloring operator equations driven by spatial white noise, with an elliptic self-adjoint pseudodifferential coloring operator from the Hörmander class. This includes the Matérn class of GRFs as a special case. Using biorthogonal multiresolution analyses on the manifold, we prove that the precision and covariance operators, respectively, may be identified with bi-infinite matrices and finite sections may be diagonally preconditioned rendering the condition number independent of the dimension p of this section. We prove that a tapering strategy by thresholding applied on finite sections of the bi-infinite precision and covariance matrices results in optimally numerically sparse approximations. That is, asymptotically only linearly many nonzero matrix entries are sufficient to approximate the original section of the bi-infinite covariance or precision matrix using this tapering strategy to arbitrary precision. The locations of these nonzero matrix entries can be determined a priori. The tapered covariance or precision matrices may also be optimally diagonally preconditioned. Analysis of the relative size of the entries of the tapered covariance matrices motivates novel, multilevel Monte Carlo (MLMC) oracles for covariance estimation, in sample complexity that scales log-linearly with respect to the number p of parameters. In addition, we propose and analyze novel compressive algorithms for simulating and kriging of GRFs. The complexity (work and memory vs. accuracy) of these three algorithms scales near-optimally in terms of the number of parameters p of the sample-wise approximation of the GRF in Sobolev scales.

Detection of a biological aerosol using optical particle counters

So far, detection and quantification of bio-aerosols requires genotypic or phenotypic identification of every single particle. Successful use of optical particle counters, as a time- and cost-saving alternative, has not been reported to date, indicating the need for further method development. Previously, such studies have focused on pollen. Here we report on a laboratory test of commercially available low-cost optical particle counters from Alphasense (OPC-N3 and OPC-R2) for the quantification of a known fungal aerosol (Botrytis cinerea). Aerosols were quantified using a Grimm Portable Aerosol Spectrometer (11E). Our measurements reveal that the low-cost sensors almost correctly detect the relative particle size distribution of the spores, as compared with the Grimm reference instrument, and potentially can be used in environmental detection of fungal aerosols.