Relative Coverage Research Articles

Insights into protein post-translational modification landscapes of individual human cells by trapped ion mobility time-of-flight mass spectrometry

Single cell proteomics is a powerful tool with potential for markedly enhancing understanding of cellular processes. Here we report the development and application of multiplexed single cell proteomics using trapped ion mobility time-of-flight mass spectrometry. When employing a carrier channel to improve peptide signal, this method allows over 40,000 tandem mass spectra to be acquired in 30 min. Using a KRASG12C model human-derived cell line, we demonstrate the quantification of over 1200 proteins per cell with high relative sequence coverage permitting the detection of multiple classes of post-translational modifications in single cells. When cells were treated with a KRASG12C covalent inhibitor, this approach revealed cell-to-cell variability in the impact of the drug, providing insight missed by traditional proteomics. We provide multiple resources necessary for the application of single cell proteomics to drug treatment studies including tools to reduce cell cycle linked proteomic effects from masking pharmacological phenotypes.

Precipitation amount and event intervals interact to change plant diversity during dry years in a desert shrubland

Variations in species diversity and soil properties in response to future climate change is a key focus area in ecological research. The influence of precipitation amount on plant community diversity and soil properties, in sandy desert ecosystems, is poorly studied. We chose the Artemisia ordosica community to examine how diversity, and the physical and chemical properties of soil vary in response to changes in precipitation. An in situ experimental platform was used to simulate total monthly precipitation at specific intervals in a desert-grassland region of northern China. The influence of precipitation on the community was investigated using three precipitation amount levels and two intervals. The relative density, coverage, and frequency of species in each experimental quadrat was analyzed for evaluating the community structure. Results showed that variability in precipitation amounts and intervals altered the species diversity in the A. ordosica communities. Increased precipitation amount enhanced whole community’s species diversity via improving the richness of perennial forbs. Lengthening the precipitation intervals enhanced whole community’s species diversity in the context of low precipitation amount but reduced species diversity in high precipitation amount condition. The precipitation patterns affected community species composition and diversity mainly by controlling soil moisture, secondly by changing the nitrogen and organic matter content of the surficial soils. This study revealed that effects of precipitation interval on the species diversity depended on annual precipitation amount and offer an innovative insight to evaluating the succession of desert ecosystems in the context of precipitation change in the future.

Bone Marrow-Free Sequencing of M Protein Genes in Monoclonal Gammopathies

Introduction In patients with monoclonal gammopathies, M proteins are patient-unique, can cause potentially fatal organ damage and can be used to track the B cell/plasma cell tumor after therapy. The presence of circulating tumor cells and the possibility to analyze the antibody repertoire through NGS and proteomics approaches may provide a window of opportunity to identify patients' specific M protein genes in the peripheral blood, without invasive bone marrow investigations. Methods Mononuclear cells from peripheral blood were subjected to single-molecule real-time sequencing of the M protein (SMaRT M-Seq)1 to obtain the circulating repertoire of the affected light chain isotype in a cohort of patients with MGUS (n=3), MM (n=8) and AL amyloidosis (n=36) at diagnosis. Tryptic digestion peptides from urinary proteins were subjected to mass spectrometry analysis to identify or validate the clonal light chain sequence from the obtained circulating repertoire2. SMaRT M-Seq on matched bone marrow samples to identify the bona fide clonal light chain sequence was performed for confirmatory purposes. Results Reads with 100% identity to the bona fide clonal light chain sequences were identified in the peripheral blood of 44 patients (94%) and were the dominant clonal sequence in 35 cases (75%). In all cases where the most abundant, discrete clonal sequence represented at least 15% of all reads detected within the peripheral blood (n=26, 55%), such sequence invariably coincided with the bona fide clonal light chain sequence as defined by bone marrow sequencing studies (Figure 1A). In a subset of cases (n=21), we also analyzed the urinary proteome and mapped tryptic digestion peptides against each patient's peripheral blood antibody repertoire. In all cases, peptide mapping correctly identified the bona fide clonal light chain sequence as the light chain sequence with the highest relative abundance, sequence coverage, and number of unique peptides among all light chain sequences identified in the patient's peripheral blood (Figure 1B). The percentage of peripheral blood sequences corresponding to the bona fide clonal light chain sequence significantly, but modestly correlated with bone marrow plasma cell infiltration (R2 0.30). Conclusion Sequencing of the circulating light chain repertoire through SMaRT M-Seq, eventually coupled with mass spectrometry-based analysis of the urinary proteome, enables the identification of the full-length clonal light chain sequence in most patients with a monoclonal gammopathy. The possibility of reliably determining disease-related immunoglobulin gene sequences even from the peripheral blood from large cohorts of patients has the potential to uncover molecular mechanisms of M protein-related clinical manifestations which have remained largely unexplored so far2. It could also facilitate approaches to personalized medicine, including the implementation of sequence-based predictive models for the identification of potentially pathogenic M proteins3,4, and the sensitive detection of patients' specific M proteins at diagnosis and MRD after anti-clonal therapy5.

The Press and South Africa's Foreign Relations

By using events data, the Afrikaans and English press has been compared for the comprehensiveness of their news coverage of South Africa's foreign relations. Events related to South Africa both as actor and target of foreign policy behaviour have been included. The findings show in broad terms that the Afrikaans press has been more comprehensive in reporting events where South Africa has been the actor. On the other hand, the English press has been far superior in their reporting events where South Africa has been the target of behaviour (external environment). These findings confirm the pluralistic nature of the press in South Africa, although some hegemonic features have been noticeable.

How School Climate Affects Student Performance: A Fuzzy Set Qualitative Comparative Analysis Based on the Perspective of Education System

School climate’s dynamic effect on student is widely explored in the study in different subjects; however, quietly, a few types of research concentrate on comparing different dimensions of school climate’s effect on student and the influence path in different countries. To analyze and compare the school climate’s mechanism in different countries’ basic education system, this article used a comparative method to explore the explanation of school climate on school students’ performance. With the application of 73 countries’ panel data from the PISA database, the author calculated the different school climate combinations’ consistency and coverage in relation to school students’ performance by fs-QCA. The author found that the punctuality of students and the classroom discipline can explain the good performance of students. It also found that the combination of multidimensional school climate has a higher explanatory power than a single factor. Finally, this study ends with countermeasures and suggestions which include improving the school climate of the regional education system and improving the utility of school climate through the appropriate mix of school climate.

Estimation of the Benthic Habitat Zonation by Photo-Quadrat Image Analysis along the Fringing Reef of Weno Island, Chuuk, Micronesia

Benthic habitat zonation is described from in situ observations and seabed photographs taken from the coastal area of Weno Island, Chuuk, Micronesia. Habitat groups, types, and boundaries are defined by visible substratum characteristics (i.e., in situ and by digital imaging of photo-quadrats along transect lines), and by cluster and ordination analyses using relative coverage percentage of identified classification categories. The statistical similarity between habitat groups is determined by Analysis of similarity (ANOSIM). Benthic habitat groups with significant influence on the determination of habitat type are isolated by the similarity percentage (SIMPER) test. In addition to the standard practices of using transect lines and collecting data in accordance with the already well-implemented and thoroughly-tested benthic habitat classification scheme for tropical reef monitoring, we applied simple statistics that enable comparative data interpretation. Our simple, repeatable methods provide a framework for benthic habitat-related monitoring research that allows the comparison of results across regions.

Decadal and seasonal changes in landcover at Padre Island: Implications for the role of the back‐barrier in signaling island state change

AbstractLong‐term and seasonal geomorphological changes at Padre Island, Texas are identified and linked with potential external drivers. Aerial and satellite images from 1950 to 2018, monthly images from 2019 to 2020, and a 2018 LiDAR data set are used to assess long‐term and seasonal geomorphological changes within a 50 km2 area of Padre Island near Port Mansfield, Texas. Trends in landcover are evaluated by mapping and comparing the relative areal coverage of each facies. Vegetated dunes, absent initially, emerged in the fore‐island and expanded into the back‐barrier to cover 14% of the study area. The active vegetation‐free back‐barrier dune field steadily decreased in areal extent from 12% to 6% as vegetation spread. Nebkha dune coverage fluctuated between 4% and 7%. Expansive microbial mats colonized the wind tidal and deflation flats surrounding the vegetated dunes and back‐barrier dune field giving rise to a remarkably different landscape over the 50‐year period studied. An assessment of external forcing factors identifies increased rates of relative sea level rise and decreased sediment influx as the most likely primary factors driving the geomorphological changes. These changes have induced a widespread shift toward stabilization of island sediments by vegetation and microbial mats, which in turn has starved the back‐barrier of sediments resulting in low rates of accretion and increased flooding. These findings highlight the sensitivity of the back‐barrier and, in particular, the dune facies to changes in sea level and sediment supply, and show that microbial mats are effective at stabilizing island sediments and may be harbingers to barrier island response to rising sea level. As shown in this study, long‐term monitoring of geomorphic facies changes and topography can detect important shifts in the island state that can be used to inform decision making for these sensitive coastal landscapes.

Cryptogams and dwarf evergreen shrubs are vulnerable to nitrogen addition in a boreal permafrost peatland of Northeast China

AbstractAimBoreal peatlands are very sensitive to nitrogen (N) enrichment due to the low soil N availability. Our aim was to reveal how increased N availability altered plant composition in boreal permafrost peatlands with dense vascular plants (>50%).LocationGreater Khingan Mountains, Northeast China.MethodsA nitrogen addition experiment with four N addition levels (0, 3, 6, and 12 g N m−2 year−1) was conducted in a poor fen in the continuous permafrost zone with vascular plant coverage above 60%. Plant diversity, community composition, and above‐ground biomass were investigated after four, six, and eight years of N addition.ResultsNitrogen addition decreased species richness and diversity but enhanced total above‐ground biomass. Plant height determined the plants’ responses to N addition, and the magnitudes increased with N addition level and experimental duration. The relative coverage of high‐stature (>50 cm) species increased, but the relative coverage of low‐stature (<50 cm) species decreased with elevating N addition levels. When N addition proceeded, the relative coverage and above‐ground biomass of tall deciduous shrubs and nitrophilous grasses increased, whereas the relative coverage and above‐ground biomass of dwarf evergreen shrubs, mosses, and lichens declined. Regardless of N addition levels, cryptogams (i.e., mosses and lichens) and a dwarf evergreen shrub (Chamaedaphne calyculata) disappeared after eight‐year N addition.ConclusionsCryptogams and dwarf evergreen shrubs are highly vulnerable to increased N availability, and N enrichment‐induced biodiversity loss, especially the disappearance of cryptogams, will deteriorate ecosystem structure and function in boreal permafrost peatlands.

The ‘morality of compromise’: David Owen, human rights diplomacy and the fall of the Pahlavi dynasty

Following his appointment as Foreign Secretary in February 1977, David Owen framed human rights promotion as a guiding principle of British foreign policy. Sensitive to Iran’s significance as a pro-Western power in the Middle East and appreciative of the opportunities that the Shah’s custom provided for British business, Owen would continue to champion human rights promotion as a central pillar of Britain’s international agenda while simultaneously providing support to the Shah’s dictatorship. This article scrutinises Owen’s attempts to rationalise this fundamental contradiction by constructing a ‘morality of compromise’ which drew inspiration, inter alia, from the value pluralism espoused by the philosopher Isaiah Berlin. In so doing, analysis not only complements and builds upon existing coverage of Anglo-Iranian relations and the marginalisation of human rights concerns therein; it also highlights the importance of moral psychology and its role in shaping ethical foreign policymaking at an individual level.

Development and Comparative Performance of Physiologic Monitoring Strategies in the Emergency Department

Accurate and timely documentation of vital signs affects all aspects of triage, diagnosis, and management. The adequacy of current patient monitoring practices and the potential to improve on them are poorly understood. To develop measures of fit between documented and actual patient vital signs throughout the visit, as determined from continuous physiologic monitoring, and to compare the performance of actual practice with alternative patient monitoring strategies. This cross-sectional study evaluated 25 751 adult visits to continuously monitored emergency department (ED) beds between August 1, 2020, and December 31, 2021. A series of monitoring strategies for the documentation of vital signs (heart rate [HR], respiratory rate [RR], oxygen saturation by pulse oximetry [Spo2], mean arterial pressure [MAP]) was developed and the strategies' ability to capture physiologic trends and vital sign abnormalities simulated. Strategies included equal spacing of charting events, charting at variable intervals depending on the last observed values, and discrete optimization of charting events. Coverage was defined as the proportion of monitor-derived vital sign measurements (at 1-minute resolution) that fall within the bounds of nursing-charted values over the course of an ED visit (HR ± 5 beats/min, RR ± 3 breaths/min, Spo2 ± 2%, and MAP ± 6 mm Hg). Capture was defined as the documentation of a vital sign abnormality detected by bedside monitor (tachycardia [HR >100 beats/min], bradycardia [HR <60 beats/min], hypotension [MAP <65 mm Hg], and hypoxia [Spo2 <95%]). Median patient age was 60 years (IQR, 43-75 years), and 13 329 visits (51.8%) were by women. Monitored visits had a median of 4 (IQR, 2-5) vital sign charting events per visit. Compared with actual practice, a simple rule, which observes vital signs more frequently if the last observation fell outside the bounds of the previous values, and using the same number of observations as actual practice, produced relative coverage improvements of 31.5% (95% CI, 30.5%-32.5%) for HR, 31.0% (95% CI, 30.0%-32.0%) for MAP, 16.8% (95% CI, 16.0%-17.6%) for RR, and 7.8% (95% CI, 7.3%-8.3%) for Spo2. The same strategy improved capture of abnormalities by 38.9% (95% CI, 26.8%-52.2%) for tachycardia, 38.1% (95% CI, 29.0%-47.9%) for bradycardia, 39.0% (95% CI, 24.2%-55.7%) for hypotension, and 123.1% (95% CI, 110.7%-136.3%) for hypoxia. Analysis of optimal coverage suggested an additional scope for improvement through more sophisticated strategies. In this cross-sectional study, actual documentation of ED vital signs was variable and incomplete, missing important trends and abnormalities. Alternative monitoring strategies may improve on current practice without increasing the overall frequency of patient monitoring.

Connectedness between the COVID-19 related media coverage and Islamic equities: The role of economic policy uncertainty

This paper examines the static and dynamic return and volatility connectedness among Islamic equity indices and a Coronavirus coverage index over the ongoing COVID-19 pandemic crisis. We employ ten major sectoral equity indices covering main economic sectors and the Coronavirus media coverage index (MCI) and apply the time-varying parameter vector autoregressive methodology (TVP-VAR). The results show a high degree of connectedness between the return and volatility series of the different sectoral indices. Moreover, the information transmission between these indices and the media coverage index shows that Islamic equities are net receivers of shocks from the coronavirus MCI. Additionally, we investigate the causality between the different connectedness measures and the Economic Policy Uncertainty (EPU). Our results indicate that EPU has predictive power on the net connectedness between the Islamic sectoral equities and the Coronavirus MCI.

Search tools and scholarly citation practices in literary studies

PurposeThe wide adoption of web-scale discovery tools calls into question the usefulness and viability of traditional subject indexes. This study examines this question of usefulness in the context of the discipline of literary studies. To what extent can researchers rely on the primary database devoted to language and literature study to discover relevant scholarship, and how does the database's performance compare to other common search tools?Design/methodology/approachThe study uses a random sample of citations from articles published in the flagship journal, PMLA, to see how well the sources cited by literature scholars are covered in various search tools, including the MLA International Bibliography.FindingsOf the search tools investigated, Google Scholar found the largest number of citations, even when limiting to literary scholarship. However, the eclecticism of citations suggests that scholars benefit from using a variety of search tools and methods.Originality/valueAlthough other studies have looked at discoverability in certain subject areas, this one focuses on literary studies. An understanding of the relative coverage of different search tools can inform librarian practices and recommendations.

An Analysis of Attitude Resources on Coverage concerning COVID-19 in New York Times

This paper adopts the attitudinal subsystem of appraisal theory to conduct a discourse analysis on news reports in the New York Times concerning COVID-19 in the initial stage. The result reveals that Appreciation resources account for the highest proportion in related coverage, while Affect and Judgement come in second and third place, respectively, with similar percentages. With regard to Affect, New York Times tends to use In/security and Dis/inclination; Judgement, capacity and Appreciation, Composition. From the perspective of the polarity of attitude, it can be seen that writers in the New York Times use both explicit and implicit attitude resources to express negative feelings toward the Chinese government’s response to this pandemic.

North Pacific and North Atlantic Jet Covariability and Its Relationship to Cool Season Temperature and Precipitation Extremes

Abstract Cool season (September–May) extreme temperature and precipitation events are frequently tied to surface cyclones and anticyclones, which are modulated on the synoptic scale by the state and evolution of the upper-tropospheric jet stream. This study adopts a jet-centered approach to classify the prevailing large-scale flow pattern into jet regimes based on the leading modes of variability of the North Pacific jet (NPJ) and the North Atlantic jet (NAJ), respectively. The characteristics of these joint NPJ–NAJ regimes are subsequently examined using composite analysis to identify large-scale flow environments conducive to the occurrence of anomalous temperatures and precipitation across North America. The analysis reveals that composite large-scale flow environments associated with each joint NPJ–NAJ regime can be approximated as a linear combination of the separate large-scale environments that characterize each NPJ regime and NAJ regime independently. Furthermore, knowledge of the joint NPJ–NAJ regime provides more precision regarding the relative likelihood and spatial coverage of anomalous temperatures and precipitation than would be obtained from consideration of the NPJ or NAJ regime in isolation. The frequencies of each joint NPJ–NAJ regime can also be modulated by the occurrence of sudden stratospheric warmings, with increased frequencies of an equatorward-shifted NAJ and a retracted NPJ during the 30-day period following a warming event. The results from the present study demonstrate that knowledge of the joint NPJ–NAJ regime exhibits the potential to inform forecasts of anomalous temperatures and precipitation at medium-range and subseasonal time scales. Significance Statement The development of cool season temperature and precipitation extremes are modulated by the state and evolution of the upper-tropospheric jet stream. Therefore, this study adopts a jet-centered approach to quantify the extent to which temperature and precipitation extremes over North America are related to the coevolution of the North Pacific and North Atlantic segments of the jet stream. The analysis demonstrates that the relative likelihood and spatial coverage of temperature and precipitation extremes varies significantly across North America based on the combined state of the North Pacific and North Atlantic jets. The jet-centered approach utilized in this study exhibits the potential to inform operational medium-range (6–10 day) and subseasonal forecasts of temperature and precipitation across North America.

Changes in the composition of the soil seed bank of grassland after giant ragweed (Ambrosia trifida L.) invasion.

Giant ragweed (Ambrosia trifida L.), an invasive weed, has an expanding distribution area and has recently started to spread in grasslands. This unusual event threatens grasslands worldwide. In this study, we aimed to evaluate the changes in the grassland soil seed banks caused by the giant ragweed invasion in Yili Valley, Xinjiang, China. Using the space-for-time substitution approach, we compared and quantified the soil seed bank communities in a grassland over eight years following giant ragweed invasion and after its removal. The results showed that the duration of invasion determined whether giant ragweed might pose a significant threat to the native seed bank community. Four years after the invasion, the in-site seed bank density of native community significantly decreased (30.44%), while the relative coverage of giant ragweed aboveground reached 83.75%. Furthermore, the species richness in the seed bank decreased significantly (12.36%), while the relative coverage of giant ragweed reached 100% six years after the invasion. Eight years after the invasion, the seed bank density and species richness of the native community decreased by 83.28% and 39.33%, respectively, whereby the seed banks tended to be homogeneous. After the removal of giant ragweed, the potential for regeneration was limited by the residual seed bank densities of the native community. Although the native seed bank density had increased significantly after three years of restoration, new growth was dominated by weedy species, rather than by the distinctive components of the grassland habitat. Our study clarifies the process by which giant ragweed causes damage to grasslands and serves as a reference for grassland restoration and management efforts.

Enantiomer Adsorption in an Applied Magnetic Field: D‐ and L‐Aspartic Acid on Ni(100)

AbstractThere have been several reports of the enantiospecific adsorption of chiral compounds from solution onto ferromagnetic surfaces in applied magnetic fields. This work studies the kinetics of D‐ and L‐aspartic acid (Asp) adsorption onto a Ni(100) surface in a 0.45 T applied magnetic field under ultra‐high vacuum (UHV) conditions. Desorption studies performed after exposing the Ni(100) sample to L‐Asp revealed no influence of the applied magnetic field on the adsorption kinetics. Similarly, concurrent exposure of the Ni(100) surface to a mixture of isotopically labeled L*‐Asp and D‐Asp resulted in no detectable field induced differences in the relative enantiomer coverages adsorbed onto the surface. In summary, the presence of an applied magnetic field had no detectable influence on the adsorption kinetics of Asp enantiomers on a Ni(100) surface under UHV conditions. This is in contrast to the magnetic field induced adsorption enantiospecificity observed in solution phase. The possible origins of this discrepancy are considered.

Health Benefits of Improved Latrine in Rural China

Proper sanitation facilities promote health because they allow people to dispose of their waste appropriately. Since the founding of the People’s Republic of China, the Chinese government has been committed to improving water and sanitation. The coverage of sanitary latrines in rural China reached more than 70.0% in 2022. Using published literature, open data, and national statistics, this study evaluated the health effects of improved latrines in China. A search strategy aimed at collecting all latrine improvement-related community intervention research in China from 1990 to 2021 was designed and implemented. Incidences of three diseases—schistosomiasis, diarrheal disease, and soil-transmitted helminths (STH)—were used as indicators of health benefits. The relationship between adjusted relative risk (RR) and coverage of sanitary latrines in the study area was examined using a log-linear model. Overall, 411 incidences of the three diseases before and after community intervention were extracted from 74 qualified articles. The results demonstrated the importance of promoting access to sanitary latrines in disease prevention and control in China. The overall estimates revealed mean reductions of 60% for diarrhea risk, 42% for schistosomiasis risk, and 65% for STH risk compared to no intervention. In addition, increasing sanitary latrine coverage was negatively correlated with RR in China, indicating that populations with high sanitary latrine coverage were less likely to be affected with water, sanitation, and hygiene-related diseases.

Design and Immunoinformatic Assessment of Candidate Multivariant mRNA Vaccine Construct against Immune Escape Variants of SARS-CoV-2.

To effectively counter the evolving threat of SARS-CoV-2 variants, modifications and/or redesigning of mRNA vaccine construct are essentially required. Herein, the design and immunoinformatic assessment of a candidate novel mRNA vaccine construct, DOW-21, are discussed. Briefly, immunologically important domains, N-terminal domain (NTD) and receptor binding domain (RBD), of the spike protein of SARS-CoV-2 variants of concern (VOCs) and variants of interest (VOIs) were assessed for sequence, structure, and epitope variations. Based on the assessment, a novel hypothetical NTD (h-NTD) and RBD (h-RBD) were designed to hold all overlapping immune escape variations. The construct sequence was then developed, where h-NTD and h-RBD were intervened by 10-mer gly-ala repeat and the terminals were flanked by regulatory sequences for better intracellular transportation and expression of the coding regions. The protein encoded by the construct holds structural attributes (RMSD NTD: 0.42 Å; RMSD RBD: 0.15 Å) found in the respective domains of SARS-CoV-2 immune escape variants. In addition, it provides coverage to the immunogenic sites of the respective domains found in SARS-CoV-2 variants. Later, the nucleotide sequence of the construct was optimized for GC ratio (56%) and microRNA binding sites to ensure smooth translation. Post-injection antibody titer was also predicted (~12000 AU) to be robust. In summary, the construct proposed in this study could potentially provide broad spectrum coverage in relation to SARS-CoV-2 immune escape variants.

Anti-Fashion Branding: Framing Technology in Uniqlo and Allbirds

Although technology has long been integral to the fashion system, executives from both Uniqlo and Allbirds are placing a renewed emphasis on it. In press interviews, company leaders argued that the clothing and shoemaker, respectively, are not fashion brands, but “technology” firms and their true competitors are the likes of Apple. Understanding that both fashion and branding are created from public discourse, this paper takes a critical discourse approach to the statements by executives, their related press coverage and their brand messaging. The paper argues that such statements discursively set the brands’ products outside of the fashion system and create their own sense of temporality. However, such statements play into contemporary discourse structures that prioritize technology as a “serious” endeavor, while concurrently degrading the associations with fashion and personal appearance. Moreover, this paper suggests that the use of framing within fashion discourses might be a productive future research endeavor.

DOMINANT INVECTIVE CONSTRUCTIONS OF MANIPULATION IN ENGLISH NEWSPAPER TEXTS

The importance of the media in relation to the problems caused by risk and uncertainty, which led to potential distortions with a significant impact on public opinion, is outlined. There is a contradiction in the hypothesis that the press in its content is a tool to encourage negative attitudes towards people who may be the probable cause of violence and a propagandist of widespread invective beliefs. The peculiarities of the reflection of the issue of migrants on the pages of English newspapers are analyzed. Examples of negative appeals in the columns of English newspapers are presented. Significant results of the research through the prism of polarized and aggressive materials of the English-language press in the context of different countries are noted. Emphasis is placed on the negative approach to the coverage of immigration and ethnic relations, as well as the reflection of the stereotypical image of a bad doctor. It is indicated that the obtained factual material of the image-stereotype structure consists of a certain set of components of nuclear and peripheral qualifiers. The most common problems in the context of the relationship in the “doctor-patient” system are highlighted. Similarities and differences in stereotypes within the analyzed linguistic cultures are revealed. The content and reasons for using the neutralization strategy to reproduce the invective are characterized. The strategy of stylistic replacement in the conditions of expediency of changing the language expression to a language function of the target culture similar in its functions is singled out. Stylistic amplification (or emphasis) and cases of its appropriate application are considered. It is noted that for the purpose of language aggression neologisms with inherent evaluation and adherent evaluation can be used. It is stipulated that the detection of invective vocabulary is relevant in the linguistic examination of controversial texts in cases of insult.