Relative Levels Research Articles

MicroRNA-20a in extracellular vesicles derived from duodenal fluid is a possible biomarker for pancreatic ductal adenocarcinoma.

Pancreatic ductal adenocarcinoma (PDAC) has a high mortality rate owing to its late diagnosis and aggression. In addition, there are relatively few minimally invasive screening methods for the early detection of PDAC, making the identification of biomarkers for this disease a critical priority. Recent studies have reported that microRNAs in extracellular vesicles (EV-miRs) from bodily fluids can be useful for the diagnosis of PDACs. Given this, we designed this study to evaluate the utility of cancer EVs extracted from duodenal fluid (DF) and their resident EV-miRs as potential biomarkers for the detection of PDAC. EV-miRs were evaluated and identified in the supernatants of various pancreatic cancer cell lines (Panc-1, SUIT2, and MIAPaca2), human pancreatic duct epithelial cells, and the DF from patients with PDAC and healthy controls. EVs were extracted using ultracentrifugation and the relative expression of EV-miR-20a was quantified. We collected a total of 34 DF samples (27 PDAC patients and seven controls) for evaluation and our data suggest that the relative expression levels of EV-miR-20a were significantly higher in patients with PDAC than in controls (p = 0.0025). In addition, EV-miR-20a expression could discriminate PDAC from control patients regardless of the location of the tumor with an area under the curve values of 0.88 and 0.88, respectively. We confirmed the presence of EVs in the DF and suggest that the expression of EV-miR-20a in these samples may act as a potential diagnostic biomarker for PDAC.

Incumbent and opposition popular support and boycotts in authoritarian and hybrid regimes in Africa, the Middle East and beyond

ABSTRACT Variation in election boycotts in Africa and the Middle East raise the question of the factors that influence the likelihood of boycotts. Examining this dynamic in Africa, the Middle East and around the globe, we explore one possible factor influencing national election boycotts: the relative level of popular support between the regime and opposition. Utilising the Varieties of Democracy (V-Dem) dataset, we find that when the regime has greater popular support than the opposition, the likelihood of major boycotts is low. In contrast, when the opposition has more popular support than the regime at the point of election, the likelihood of boycotts increases. These findings highlight the importance of publicly observable levels of support as a means for opposition parties to gauge the possible payoff for boycotting, particularly in authoritarian and hybrid regimes where lack of transparency creates uncertainty regarding other sources of regime strength or weakness.

Housing and Health in Older Adults With Low Income in the United States: An Integrative Review.

The current integrative review was conducted to understand the relationship between housing and health in older adults with low income in the United States. A literature search yielded 20 articles that met inclusion criteria. Key data elements were extracted from each article and a five-level social ecological model (SEM) was used as a framework to analyze the findings. The analysis yielded themes associated with each SEM level: Interaction Between Housing and Personal Traits and Behaviors (individual level); Burdens and Benefits of Social Relationships (relational Level); Building Quality and Health (environmental level); Role of Housing Assistance (structural level); and Influence of Poverty and Structural and Systemic Racism (superstructural level). Results clarify housing's role as a social determinant of health affecting older adults with low income and may help nurses tailor patient assessments and treatment plans to better identify and address housing-related health risks. [Journal of Gerontological Nursing, 50(3), 25-32.].

Combination of Qingfei Huayu Decoction and Targeted Nursing Care Improves the Clinical Outcomes in Pediatric Patients with Mycoplasma Pneumoniae Pneumonia

To explore the efficacy of the combined use of Qingfei Huayu decoction (QFHYD) and targeted nursing care in pediatric cases of Mycoplasma Pneumoniae pneumonia (MPP). One hundred and twenty pediatric cases of MPP admitted to our hospital from January 2021 to December 2022 were selected and randomly divided into control (n = 60) and experimental (n = 60) groups. The control group (CG) was treated with targeted nursing care in conjunction with conventional Western medicine, while the experimental group (EG) received QFHYD in addition to the CG’s treatment. Clinical efficacy, symptom relief time, and levels of inflammatory markers were recorded. Subsequently, a mouse model of MPP was established to investigate the effects of QFHYD on bacterial load in lung tissues, tissue damage, and the expression of members of the Notch pathway. The symptom relief times for cough, fever, and pulmonary rales were shorter in the EG than in the CG (all P < 0. 001). After treatment, interleukin-6 (IL-6) and IL-8 levels decreased in both groups, with the levels being lower in the EG (both P < 0. 001). Mouse model results showed that both azithromycin alone and in combination with QFHYD could reduce bacterial loads in bronchoalveolar lavage fluid and lung injury scores (all P < 0. 001), with the combination showing more significant effects. Furthermore, western blotting indicated significant reductions in the relative protein levels of Notch1 and Notch2 in mouse lung tissues, with the combination showing more significant effects (all P < 0. 001). In pediatric MPP, the combination of QFHYD and targeted nursing care can significantly enhance clinical efficacy, accelerate symptom relief, and improve the body’s inflammatory state. Based on animal studies, we speculate that the action of QFHYD may be related to the Notch pathway.

Exosomal miR-223 promotes ARDS by targeting insulin-like growth factor 1 receptor: A cell communication study

Background Acute respiratory distress syndrome (ARDS) is a respiratory failure syndrome characterized by hypoxemia and changes in the respiratory system. ARDS is the most common cause of death in COVID-19 deaths was ARDS. In this study, we explored the role of miR-223 in exosomes in ARDS. Methods Exosomes were purified from the supernatants of macrophages. qPCR was used to detect relative mRNA levels. A luciferase reporter assay was performed to verify the miRNA target genes. Western blotting was used to detect the activation of inflammatory pathways. Flow cytometry was performed to assess apoptosis. An LPS-induced ARDS mouse model was used to assess the function of miR-223 in ARDS. Results Exosomes secreted by macrophages promoted apoptosis in A549 cells. Macrophages and exosomes contain high levels of miR-223. Exogenous miR-223 can decrease the expression of insulin-like growth factor 1 receptor (IGF-1R) in A549 and promote the apoptosis of A549.Transfection of anti-miR223 antisense nucleotides effectively reduced the level of miR-223 in macrophages and exosomes and eliminated the pro-apoptotic effect of A549. In vivo, LPS stimulation increased inflammatory cell infiltration in the lungs of mice, whereas knockdown of miR-223 in mice resulted in significantly reduced eosinophil infiltration. Conclusions Macrophages can secrete exosomes containing miR-223 and promote apoptosis by targeting the IGF-1R/Akt/mTOR signaling pathway in A549 cells and mouse models, suggesting that miR-223 is a potential target for treating COVID-19 induced ARDS.

Medicinal Plants Used by Oromo Community in Kofale District, West-Arsi Zone, Oromia Regional State, Ethiopia.

The purpose of this study was to record the utilization of medicinal plants by the Oromo people in the Kofale District, Oromia Regional State, Ethiopia, to control human and animal health problems. Data regarding the use of medicinal plants were collected using ethnobotanical methods from 84 traditional medicine practitioners and 304 general informants sampled employing purposive and systematic random sampling methods, respectively, in the Kofale District. Data were analyzed using different indices, including a preference ranking exercise, informant consensus factor (ICF), fidelity level (FL) and relative popularity level (RPL). In the district, 106 medicinal plants were claimed to be used for the treatment of 43 human and 18 livestock illnesses, of which 75 (71%) were used to manage human health problems, 23 (21.5%) were used to treat both human and livestock ailments and eight (7.5%) were utilized to treat manage livestock health problems. Most (76.4%) plants were harvested from the wild. Leaves were the most commonly used plant part (55.6%) in remedy preparations. Skin diseases scored the highest ICF value (0.97), followed by gastrointestinal disorders (ICF = 0.95), cancer (ICF = 0.93), and hemorrhoids (ICF = 0.91). Medicinal plants that record the highest fidelity level (FL) (100%) and rank order priority (ROP) (100%) values included Justicia schimperiana, Embelia schimperi, Ekebergia capensis and Datura stramonium, which have been used to treat liver disorders, tapeworm infections, babesiosis, and rabies, respectively. There were significant differences (p<0.05) in the mean numbers of medicinal plants claimed by different social groups: older, illiterate, and traditional medicine practitioners reported higher mean numbers of medicinal plants than younger, literate, and general informants, respectively. This study indicated the richness of medicinal plant species in Kofale District. Medicinal plants with the highest FL and ROP values and those used to treat disease categories with the highest ICF values should be prioritized in future phytochemical and pharmacological investigations.

Anti-Inflammatory Effect of Chamaecyparis obtusa (Siebold & Zucc.) Endl. Leaf Essential Oil.

Chamaecyparis obtusa (Siebold & Zucc.) Endl. (C. obtusa) belongs to the Cupressaceae family and is native to East Asian regions. Essential oils extracted from the leaves, bark, branches, and roots of C. obtusa have both aesthetic and medicinal properties and are thus widely used. However, detailed analyses of the active ingredients of C. obtusa extract are lacking. In this study, the sabinene content in the hydro-distillation of C. obtusa leaf essential oil (COD) was analyzed using GC-MS, and the anti-inflammatory effect of COD was compared with that of pure sabinene. Cell viability was evaluated by MTT assay, and nitric oxide (NO) production was measured using Griess reagent. Relative mRNA and protein levels were analyzed using RT-qPCR and western blot, and secreted cytokines were analyzed using a cytokine array kit. The results showed that both COD and sabinene inhibited the expression of inducible nitric oxide synthase (iNOS) and the phosphorylation of c-Jun N-terminal kinase (JNK) and p38 in lipopolysaccharide (LPS)-induced RAW 264.7 cells. COD and sabinene also reduced the production of pro-inflammatory cytokines interleukin (IL)-1β, IL-6, IL-27, IL-1 receptor antagonist (IL-1ra), and granulocyte-macrophage colony-stimulating factor (GM-CSF). The anti-inflammatory mechanisms of COD and sabinene partially overlap, as COD was shown to inhibit MAPKs and the JAK/STAT axis, and sabinene inhibited MAPKs, thereby preventing LPS-induced macrophage activation.

Conservation energetics of beluga whales: using resting and swimming metabolism to understand threats to an endangered population.

The balance between energetic costs and acquisition in free-ranging species is essential for survival, and provides important insights regarding the physiological impact of anthropogenic disturbances on wild animals. For marine mammals such as beluga whales (Delphinapterus leucas), the first step in modeling this bioenergetic balance requires an examination of resting and active metabolic demands. Here, we used open-flow respirometry to measure oxygen consumption during surface rest and submerged swimming by trained beluga whales, and compared these measurements with those of a commonly studied odontocete, the Atlantic bottlenose dolphin (Tursiops truncatus). Both resting metabolic rate (3012±126.0 kJ h-1) and total cost of transport (1.4±0.1 J kg-1m-1) of beluga whales were consistent with predicted values for moderately sized marine mammals in temperate to cold-water environments, including dolphins measured in the present study. By coupling the rate of oxygen consumption during submerged swimming with locomotor metrics from animal-borne accelerometer tags, we developed predictive relationships for assessing energetic costs from swim speed, stroke rate and partial dynamic acceleration. Combining these energetic data with calculated aerobic dive limits for beluga whales (8.8 min), we found that high-speed responses to disturbance markedly reduce the whale's capacity for prolonged submergence, pushing the cetaceans to costly anaerobic performances that require prolonged recovery periods. Together, these species-specific energetic measurements for beluga whales provide two important metrics, gait-related locomotor costs and aerobic capacity limits, for identifying relative levels of physiological vulnerability to anthropogenic disturbances that have become increasingly pervasive in their Arctic habitats.

Color measurements of the polarized light scattered by the dust in protoplanetary disks

Context. Many reflected light images of protoplanetary disks have been obtained with ground-based high-contrast instruments. Quantitative measurements of the reflected radiation provide strong constraints on the scattering dust which can clarify the dust particle evolution in these disks and the composition of the forming planets. Aims. We derived the wavelength dependence of the polarized reflectivity (Qˆφ/I*)λ or color for a sample of disks to contrain the dust based on these data. Further we searched for systematic differences in the dust properties between subgroups of disks. Methods. We selected from the ESO archive polarized imaging for 11 protoplanetary disks obtained at visible and near-infrared wavelengths with the SPHERE/ZIMPOL and SPHERE/IRDIS high contrast instruments. All disks have bright and well-resolved structures, such as rings or spirals, which allow accurate determinations of the intrinsic polarized reflectivity Qˆφ/I* at multiple wavelengths. For this, we corrected the observations for the smearing effects caused by the point spread functions (PSFs) of the individual dataset with a novel correction procedure applicable to inclined disks. For the 11 disks, we derived a total of 31 Qˆφ/I* values for wavelengths from 0.62 μm (R band) to 2.2 μm (Ks band) and compared our results, if possible, with previous determinations. For each disk, we derived a polarized reflectivity color ηV/IR between a visible band λ &lt; 1 μm and a near-IR band λ &gt; 1 μm and other wavelength combinations. We also consider model calculations for the polarized reflectivity colors η for protoplanetary disks to constrain the scattering properties of the dust. Results. The polarized reflectivities for the investigated disks structures are at a typical level between Qφ/I★ ~ 0.1% to 1.0%. These values depend strongly on the observing conditions and a correction for the PSF smearing effects is essential to obtain the intrinsic values Qˆφ/I*. Corrected values Qˆφ/I* are on average about a factor of 1.6 higher than the observed values. We checked the accuracy of the PSF calibrations procedure with simulations and literature data, and show that the large systematic errors in the observational values are reduced to a relative level ΔQˆφ/Qˆφ ≈ 10% or even less. The high accuracy yields wavelength gradients for the polarized reflectivity (Qˆφ/I*)φ or colors η which are significantly different between different objects. We find in our sample for all disks around Herbig stars (HD 169142, HD 135344B, HD 100453, MWC 758, and HD 142527) a red color ηv/IR &gt; 0.5, while four out of six disks around T Tauri stars (PDS 70, TW Hya, RX J1615, and PDS 66) are gray −0.5 &lt; ηV/IR &lt; 0.5. The red colors support the presence of rather compact dust grains, while the absence of blue colors (except for the near-infrared color of PDS 66) is not compatible with very porous aggregates composed of small monomers. We suspect, that the very red colors ηV/IR ≈ 2 obtained for LkCa 15 and MWC 758 could be the result of an "extra" reddening of the radiation illuminating the disk caused by absorbing hot dust near the star. We discuss the prospects of further improvements for the derivation of dust properties in these disks if the fractional polarization 〈pψ〉 or other parameters of the reflected light are also taken into account in future studies.

Effect of total flavonoids of hawthorn leaves on H2O2-induced oxidative stress injury of rats

This study was to investigate the role of total flavonoids of hawthorn leaves (TFHL) in oxidative stress injury in rats with diabetic nephropathy (DN). CCK-8 method was used to study the effect of TFHL on the oxidative damage of kidney HK-2 cells induced by H2O2. 40 Sprague-Dawley (SD) rats with DN induced by streptozotocin were divided into normal saline group and treatment groups treated with TFHL (10, 20, 40 mg/kg). During the 6 weeks of treatment, metabolic parameters such as body weight and fasting blood glucose levels were recorded along with analysis of renal function, levels of renal tissue apoptosis, and fibrosis-related factors. The relative levels of Nrf-2/ARE pathway-related factors in kidney tissue were detected by qPCR and Western blot. 320 μg/mL TFHL significantly improved the survival rate of HK-2 cells with oxidative damage (92.9%). In treatment group, GPX1, SOD2 and GSH were significantly up-regulated, leading to down-regulation of ROS. In addition, after administration for 6 weeks, TFHL improved the renal function of DN rats and worsened the morphology of nephropathy. They also improved Bcl-2, Nrf-2, NQO-1 and HO-1 levels, and regulated Nrf-2/ARE signaling, thus having a protective effect on the kidney tissue of DN rats. After TFHL acted on kidney cells, the nuclear expression of Nrf-2 protein was significantly increased. TFHL pretreatment up-regulated NQO-1, Nrf-2 and HO-1. Our data showed that in DN rats, TFHL protected renal cells and alleviated renal tissue damage.

Antifibrotic effects of colchicine on 3T3 cell line ischemia to mitigate detrimental remodeling

Context: Acute myocardial infarction (AMI) is one of the clinical manifestations of coronary heart disease caused by the cessation of coronary blood flow. The development of the management of AMI has resulted in a reduction in the mortality rate of AMI patients. Nevertheless, the apparent inflammation triggers detrimental remodeling, so antifibrotic such as colchicine are needed. Aims: To analyze the impact of colchicine administration on the reduction of ventricular remodeling through the NLRP3 inflammasome, TGF-β, and α-SMA in a 3T3 cell line culture under ischemic conditions. Methods: The 3T3 cell line culture underwent ischemia treatment using CoCl2 and subsequent treatment with colchicine. This treatment was conducted for 24 hours with a concentration of 300 μM CoCl2, followed by the administration of colchicine at a concentration of 1 μM after the ischemia treatment. The expression levels of NLRP3, TGF-β, and α-SMA were assessed using flow cytometry, and the data were analyzed through one-way ANOVA. Results: Administration of colchicine for 24 hours following ischemia resulted in a significant decrease in the relative levels of NLRP3, TGF-β, and α-SMA (p&lt;0.05) compared to the ischemia group. Conclusions: In vitro administration of colchicine can reduce post-ischemic remodeling, including the NLRP3 inflammasome, TGF-β, and α-SMA.

Construction of a miR-15a-based risk prediction model for vascular calcification detection in patients undergoing hemodialysis

Vascular calcification (VC) is highly prevalent in patients undergoing hemodialysis, and is a significant contributor to the mortality rate. Therefore, biomarkers that can accurately predict the onset of VC are urgently required. Our study aimed to investigate serum miR-15a levels in relation to VC and to develop a predictive model for VC in patients undergoing hemodialysis at the Beijing Friendship Hospital hemodialysis center between 1 January 2019 and 31 December 2020. The patients were categorized into two groups: VC and non-VC. Logistic regression (LR) models were used to examine the risk factors associated with VC. Additionally, we developed an miR-15a-based nomogram based on the results of the multivariate LR analysis. A total of 138 patients under hemodialysis were investigated (age: 58.41 ± 13.22 years; 54 males). VC occurred in 79 (57.2%) patients. Multivariate LR analysis indicated that serum miR-15a, age, and WBC count were independent risk factors for VC. A miR-15a-based nomogram was developed by incorporating the following five predictors: age, dialysis vintage, predialysis nitrogen, WBC count, and miR-15a. The receiver operating characteristic (ROC) curve had an area under the curve of 0.921, diagnostic threshold of 0.396, sensitivity of 0.722, and specificity of 0.932, indicating that this model had good discrimination. This study concluded that serum miR-15a levels, age, and white blood cell (WBC) count are independent risk factors for VC. A nomogram constructed by integrating these risk factors can be used to predict the risk of VC in patients undergoing hemodialysis.

MiR-335-3p improves type II diabetes mellitus by IGF-1 regulating macrophage polarization.

Previous studies have found that miR-335 is highly expressed in type II diabetes mellitus (T2DM) models and is related to insulin secretion, but there are few studies on the regulatory effects of miR-335-3p on insulin resistance and macrophage polarization in T2DM patients. This study aims to explore the effects of miR-335-3p on insulin resistance and macrophage polarization in T2DM patients. Blood glucose (insulin tolerance tests, glucose tolerance tests) and body weight of the T2DM model were measured; macrophages from adipose tissue were isolated and cultured, and the number of macrophages was detected by F4/80 immunofluorescence assay; the Real-time quantitative polymerase chain reaction (qPCR) assay and Western blot assay were used to detect the miR-335-3p expression levels, insulin-like growth factor 1 (IGF-1), M1-polarizing genes (inducible nitric oxide synthase [iNOS] and TNF-α) as well as M2-polarizing genes (IL-10 and ARG-1). The targeting link between miR-335-3p and IGF-1 was confirmed using bioinformatics and dual luciferase assay. The results showed that miR-335-3p expression level in adipose tissue of the T2DM model was significantly decreased, and the mice's body weight and blood glucose levels dropped considerably, miR-335-3p inhibited the number of macrophages, inhibiting the iNOS and TNF-α relative mRNA expression levels, and up-regulated the IL-10 and ARG-1 relative mRNA expression levels, miR-335-3p negatively regulated target gene IGF-1, IGF-1 significantly increased the iNOS and TNF-α mRNA and protein expression levels, decreasing the IL-10 and ARG-1 mRNA and protein expression levels, indicating that miR-335-3p could affect the T2DM process by regulating macrophage polarization via IGF-1.

Academic Stress and Study Habits in Peruvian University Students

The current research aims to analyze the relationship between academic stress and study habits in Peruvian university students. A quantitative method was employed, with a non-experimental design, basic research type, and relational level. The study was conducted with a population of 133 students from various specialties at the Faculty of Education of the Catholic University of Santa María in Arequipa, selecting a sample of 99 students for the study. For data collection, two instruments were applied: the Likert scale questionnaire called SISCO Inventory, consisting of 29 questions to assess the level of stress, and another Likert scale questionnaire, called Study Habits Inventory, comprising 53 questions to evaluate the knowledge about study habits. After conducting the correlation between the variables under study, it was determined that there is a very strong inverse correlation between academic stress and study habits, approaching a perfect correlation. The correlation coefficient obtained was r^2 = (-0.840)^2 = 0.706. This indicates that 70.6% of academic stress affects the study habits of students at the School of Education of the aforementioned university. The significance level of this correlation is very strong, implying that the higher the level of academic stress, the worse the study habits of the students, and vice versa.

Research on spatiotemporal distribution characteristics of ship traffic flow in port waters based on AIS data

With the gradual increase of ship traffic, water traffic management has become increasingly important. This paper is based on AIS data and uses the Analytic Hierarchy Process to comprehensively evaluate the relative hazard level of a specific area, in order to determine the relative consequences of regional hazards (RCORH). Next, we will use speed and traffic volume characteristics as indicators to construct a linear regression model for spatial relationship analysis. The research results indicate that the linear regression model proposed in this article can effectively identify areas with high collision risks, which has positive significance for improving the navigation safety management of port waters.

Environmental causality calibration: Advancing WLAN RF fingerprinting for precise indoor localization.

In recent years, considerable and valuable research progress has been made in indoor positioning technologies based on WLAN Radio Frequency (RF) fingerprinting, identifying it as one of the most promising positioning technologies with substantial potential for wider adoption. However, indoor environmental factors significantly influence the propagation of wireless RF signals, resulting in a considerable decrease in positioning accuracy as the indoor environmental conditions vary. Thus, effectively mitigating the impact of indoor environmental factors on WLAN RF fingerprinting-based positioning systems has become a crucial research problem. Currently, there is a dearth of comprehensive research on the influence of indoor climatic factors, particularly the variations in relative humidity, on the propagation of WLAN RF signals within indoor spaces and its consequential impact on positioning accuracy. To address the aforementioned issues, this paper proposes an Adaptive expansion fingerprint database (AeFd) model based on a regression learning algorithm. The AeFd, through the design of a relationship model describing the interaction between fingerprint databases under varying relative humidity, allows the fingerprint database expanded by AeFd to dynamically adapt to the changes in indoor relative humidity. Our experiments show that using the AeFd model with the KNN algorithm, a 5% performance improvement was observed over 10 days and an 8% improvement over 10 months. According to experimental test results, the fingerprint database expansion model AeFd proposed in this paper can effectively expand the fingerprint database under different relative humidity levels, thereby significantly enhancing the positioning performance of the system and improving its stability.

Accuracy and validity of common dental terms using chat GPT model: a cross-sectional study

Background: Chat generative pre-trained transformer, an artificial intelligence chatbot can generate text-based content for information purpose. This study aims to find the accuracy and reliability of the chat GPT generated definitions for 30 common dental terms. Methods: A 15 current dental teaching staffs grading from Professors and Readers of various specialities participated in this study. They graded the chat GPT generated terms on a 5-point Likert scale (1- Strongly disagree, 2- Disagree, 3- Neutral, 4- Agree, 5- Strongly disagree). Scores were obtained and descriptive statistics was done and compared using Mann-Whitney U test. Results: Among 30 dental terms, 13 terms which were generated from the chat GPT model were found to be more appropriate when compared to text book definition. On comparison of reviewers’ perceptions for accuracy of definitions generated from chat GPT compared with text book definitions in which among the 30 dental terms, 9 terms were found to be statistically significant (p&lt;0.05*). Conclusions: Chat GPT is a potential tool for answering knowledge based questions with equal vigor in the field of dentistry. Moreover, the accuracy of Chat GPT to solve questions in dentistry has a relational level of accuracy.

Proximate and fatty acids composition in the muscle of wild and farmed sardine (Sardinella brasiliensis)

Wild sardines contain a high level (2560 mg/100 g n-3 LC-PUFA of wet mass) of long-chain (≥C20) polyunsaturated fatty acids (LC-PUFA), namely, PUFA with 20 and 22 carbon atoms, however, in captivity their fatty acid composition can be affected by food and farming conditions. Therefore, this study evaluated the proximate composition and fatty acid profile of sardines cultivated in the four seasons of the year as well as compared to those captured in the wild in the spring. In both analyses, farmed sardines differed significantly from wild specimens. Total lipid content was significantly higher, especially in winter, with 14.52 % and 2.37 % for farmed and wild sardines, respectively. The fatty acid profile had a prevalence of n-3 LC-PUFA in wild fish (17.6 %) and n-6 PUFA ​​in farmed fish (12.2 %). LC-PUFA showed higher relative levels in wild animals, 16.4 % against 1.2 % for farmed sardines in winter. In absolute terms, the amount of EPA (eicosapentaenoicacid) and DHA (docosahexaenoicacid) in farmed sardine was similar to the wild specimens in spring 4000 mg/100 g and 3000 mg/100 g, respectively.

Evaluation of carcass traits, meat quality and the expression of lipid metabolism-related genes in different slaughter ages and muscles of Taihang black goats.

This study was conducted to investigate the effect of slaughter age on carcass traits, meat quality, and the relative mRNA levels of lipid metabolism-related genes in different muscles of Taihang black goats. In this study, the triceps brachii (TB), longissimus dorsi (LD) and gluteus (GL) muscles of 15 grazing Taihang black goats slaughtered at the age of 2, 3, and 4 (designated as 2-year-old, 3-year-old, and 4-year-old, respectively) were collected. The differences in carcass shape, meat quality, amino acid composition and lipid metabolism gene expression among Taihang black goats of different ages and from different plant parts were compared. Compared with goats at other ages, goats slaughtered at the age of 4 had greater live and carcass weights, meat weights, bone weights and skin areas (p<0.05). LD in the 4-years-old had the lowest cooking loss and moisture content. The crude protein content in the LD of 2-year-old was significantly greater than that in the other age group, and at the age of 2, the LD had the highest crude protein content than TB and GL. The highest fat content was in LD, followed by TB, for goats slaughtered at the age of 4. Eight out of 9 essential amino acids had higher content in the TB compared with other muscles, regardless of age. The total essential amino acid content was highest in the 4-year-old and lowest in the GL muscle at the age of 3. The sterol regulatory element-binding protein-1c (SREBP-1c) and adipose triglyceride lipase (ATGL) genes were significantly more abundant in the TB muscle than in the other muscles for goats slaughtered at the age of 2. At the age of 4, the ATGL and peroxisome proliferator-activated receptor γ (PPARγ) genes were significantly more abundant in the GL than in the LD, while the fatty acid synthase (FAS) genes were significantly less abundant in the GL than in the other muscles. Similarly, compared with those in goats of other ages, the relative mRNA expression levels of the FAS and heart-type fatty acid binding protein (H-FABP) genes in goats slaughtered at the age of 4 were the highest, and the relative mRNA expression of the PPARγ gene was the lowest (p<0.05). The relative mRNA expression of the H-FABP and FAS genes was positively correlated with the intramuscular fat (IMF) content, while the relative mRNA expression levels of the PPARγ and ATGL genes was negatively correlated with the IMF content. Overall, a better nutritional value was obtained for TB from 4-year-old goats, in which the total essential amino acid and fat contents were greater than those of other muscles. The comprehensive action of lipid metabolism genes was consistent with that of the IMF content, among which the FAS, H-FABP, PPARγ, and ATGL genes had positive and negative effects on the process of IMF deposition in Taihang black goats.

Synergistic promotion of transient transgene expression in CHO cells by PDI/XBP-1s co-transfection and mild hypothermia.

Transient gene expression system is an important tool for rapid production of recombinant proteins in Chinese hamster ovary (CHO) cells. However, their low productivity is the main hurdle to overcome. An effective approach through which to obtain high protein yield involves targeting transcriptional, post-transcriptional events (PTEs), and culture conditions. Here, we investigated the effects of protein disulfide isomerase (PDI) and spliced X-box binding protein 1 (XBP-1s) co-overexpression combined with mild hypothermia on the transient yields of recombinant proteins in CHO cells. The results showed that the gene of interest (GOI) and the PDI/XBP-1s helper vector at a co-transfection ratio of 10:1 could obviously increase transient expression level of recombinant protein in CHO cells. However, PDI/XBP-1s overexpression had no significance effect on the mRNA levels of the recombinant protein, suggesting that it targeted PTEs. Moreover, the increased production was due to the enhancing of cell specific productivity, not related to cell growth, viability, and cell cycle. In addition, combined PDI/XBP-1s co-overexpression and mild hypothermia could further improve Adalimumab expression, compared to the control/37°C and PDI/XBP-1s/37°C, the Adalimumab volume yield of PDI/XBP-1s/33°C increased by 203% and 142%, respectively. Mild hypothermia resulted in 3.52- and 2.33-fold increase in the relative mRNA levels of PDI and XBP-1s, respectively. In conclusion, the combination of PDI/XBP-1s overexpression and culture temperature optimization can achieve higher transient expression of recombinant protein, which provides a synergetic strategy to improve transient production of recombinant protein in CHO cells.