A quartz fixed-bed, microreactor was successfully constructed for both the catalytic methanol-to-gasoline (MTG) and methanol-and-glycerol-to-gasoline (MGTG) processes. The reaction products were analyzed by gas chromatography–mass spectrometry (GC–MS) and high-performance liquid chromatography (HPLC). Process variables of temperature and reaction time were studied to determine the effects on conversion rates, product yield, and ZSM-5 catalyst lifetime for both systems. Moreover, the factor of glycerol additions (10, 25, and 40% in methanol) was also investigated for the MGTG process. The MTG and MGTG generated oil phase showed a similar composition, mainly methylbenzenes, to regular gasoline, and composition changed as the reaction proceeded to favor heavier aromatics. In the MTG process, the best catalytic performance was achieved at 425 °C, at which the product yield and catalyst lifetime were 11.0 wt % and 20 h, respectively. Generally, the methanol conversion rate and the total liquid and organic-phas...