Regions Of Theworld Research Articles

Complementarity of Sentinel-1 and Sentinel-2 Data for Soil Salinity Monitoring to Support Sustainable Agriculture Practices in the Central Bolivian Altiplano

Soil salinization will affect 50% of global cropland areas by 2050 and represents a major threat to agricultural production and food sovereignty. As soil salinity monitoring is costly and time consuming, many regions of the world undertake very limited soil salinity observation (in space and time), preventing the accurate assessment of soil salinity hazards. In this context, this study assesses the relative performance of Sentinel-1 radar and Sentinel-2 optical images, and the combination of the two, for monitoring changes in soil salinity at high spatial and temporal resolution, which is essential to evaluate the mitigation measures required for the sustainable adaptation of agriculture practices. For this purpose, an improved learning database made of 863 soil electrical conductivity (i.e., soil salinity) observations is considered for the training/validation step of a Random Forest (RF) model. The RF model is successively trained with (1) only Sentinel-1, (2) only Sentinel-2 and (3) both Sentinel-1 and -2 features using the Genetic Algorithm (GA) to reduce multi-collinearity in the independent variables. Using k-fold cross validation (3-fold), overall accuracy (OA) values of 0.83, 0.88 and 0.95 are obtained when considering only Sentinel-2, only Sentinel-1 and both Sentinel-1 and -2 features as independent variables. Therefore, these results highlight the clear complementarity of radar (i.e., Sentinel-1) and optical (i.e., Sentinel-2) images to improve soil salinity mapping, with OA increases of approximately 10% and 7% when compared to Sentinel-2 and Sentinel-1 alone. Finally, pre-sowing soil salinity maps over a five-year period (2019–2023) are presented to highlight the benefit of the proposed procedure to support the sustainable management of agricultural lands in the context of soil salinization on a regional scale.

The mediation role of innovation in the relationship between total quality management and performance of small and medium scale enterprises

PurposeThis study examined the mediation-moderation role of innovation and market dynamism in the association between total quality management (TQM) practices and the performance of small and medium-scale enterprises' (SMEs') performance with empirical evidence from sub-Saharan Africa.Design/methodology/approachUsing a questionnaire, the research model developed was tested with responses from 203 owners and managers of SMEs in Ghana. The analyses were done using Statistical Package for the Social Sciences (SPSS) and Smart Partial Least Squares Structural Equation Modeling (PLS-SEM).FindingsThe innovation initiatives partially and fully mediated the relationship between TQM practices and the performance of SMEs. Also, the indirect effect of TQM practices of SMEs on performance through innovation initiatives was negatively moderated by market dynamism.Practical implicationsThe study contributes to the TQM literature by validating the indirect and direct relationship between TQM practices and performance in the context of SMEs in a developing region.Originality/valueThis paper presents a novel understanding of the relationship between TQM and SMEs in developing regions of the world. The paper serves as a guide for SME owners and managers to improve the performance of their organizations through TQM practices.

Modern biological methods of processing plant raw materials used to increase its storage capacity

Foodborne illnesses, mainly infectious, are a leading cause of morbidity and mortality worldwide. Pathogenic bacteria are present at virtually every stage of the food production chain, compromising company food safety programs and causing out-breaks of foodborne illnesses in various regions of the world. Finding new solutions that provide adequate microbiological stability to minimally processed foods is key to controlling bacterial pathogens that cause foodborne illnesses. The use of chemical and physical methods of food preservation often leads to a deterioration in their nutritional value, physical and organoleptic properties. Minimally processed foods produced without any radical preservation methods may be at particular risk of developing microorganisms, including pathogens. Low-temperature production processes and refrigerated storage promote the development of psychrophilic microorganisms; another threat is posed by high microbiological contamination of raw materials. To preserve the quality of food products, the most commonly used physicochemical methods include modified atmosphere packaging, membrane methods or ultrasound. Alternatively, biological methods can be used: bacteriophages and phage cocktails, bacteriocins, inactivation of plant tissue degradation enzymes, phytochemicals, edible coatings. Moreover, they can be used either individually to limit the growth of bacteria in the food environment, or in combination with other methods in order to achieve maximum effect. This article discusses the main biological methods of combating pathogenic bacteria most commonly found in the food environment. The purpose of this review was to consider existing biological methods for processing plant objects, as well as to identify the advantages and disadvantages of each method.

The virome of bubaline (Bubalus bubalis) tonsils reveals an unreported bubaline polyomavirus.

Water buffalo (Bubalus bubalis) farming is increasing in many regions of the world due to the species' ability to thrive in environments where bovine cattle would struggle. Despite water buffaloes being known for their resistance to diseases, there is a lack of data about the diversity of the microbiome of the species. In this study, we examined the virome diversity in palatine tonsils collected from animals from the island of Marajó, northern Pará state, Brazil, which harbors the largest bubaline flock in the country. Tonsil fragments from 60 clinically healthy bubalines were randomly selected from a sample of 293 animals. The samples were purified, extracted, and randomly amplified with phi29 DNA polymerase. After amplification, the products were purified and sequenced. Circular DNA viruses were predominant in the tonsils' virome. Sequences of genome segments representative of members of the genera Alphapolyomavirus (including a previously unreported bubaline polyomavirus genome) and Gemycircularvirus were identified, along with other not yet classified circular virus genomes. As the animals were clinically healthy at the time of sampling, such viruses likely constitute part of the normal tonsillar virome of water buffaloes inhabiting the Ilha do Marajó biome.

Airway breathing circulation dengue: a case of multifactorial shock due to major trauma and severe dengue infection

BackgroundDengue is the most common arboviral illness reported globally, endemic to most tropical and sub-tropical regions of the world. Dengue Shock Syndrome is a rare complication of severe Dengue infection resulting in haemorrhagic complications and refractory hypotension. We report on a case of severe dengue diagnosed in a patient with major trauma and illustrate some of the potential challenges and considerations in the clinical management of such cases.Case PresentationA 49-year-old female presented following a road trauma incident with multiple abdominal injuries requiring urgent laparotomy. Her recovery in Intensive Care Unit was complicated by the development of Dengue Shock Syndrome characterised by a falling haemoglobin and platelet count, multiorgan dysfunction and prolonged hospital stay.ConclusionsDengue Shock Syndrome may complicate fluid management and bleeding control in major trauma cases. Awareness of Dengue, particularly in endemic areas and returned travellers may help facilitate early diagnosis and management of complications.

International Council for Standardization in Haematology (ICSH) recommendations for the performance and interpretation of activated partial thromboplastin time and prothrombin time mixing tests.

This guidance document has been prepared on behalf of the International Council for Standardization in Haematology (ICSH). The aim of the document is to provide guidance and recommendations for the performance and interpretation of activated partial thromboplastin time (APTT) and prothrombin time (PT) plasma mixing tests in clinical laboratories in all regions of the world. The following areas are included in this document: preanalytical, analytical, postanalytical, and quality assurance considerations as they relate to the proper performance and interpretation of plasma mixing tests. The recommendations are based on good laboratory practice, published data in peer-reviewed literature, and expert opinion.

A multifaceted ecological approach to explore links between environmental factors and the epidemiology of disorders of gut-brain interaction.

Disorders of gut-brain interaction (DGBI) are characterized by debilitating symptoms not explained by structural or biochemical abnormalities. While functional conditions present with complex, likely heterogeneous pathophysiology, we aimed to investigate if proxy measures of sociocultural and environmental factors are associated with the prevalence of various DGBI in populations across the world. We performed an ecological study utilizing peer-reviewed published datasets reporting for 26 countries prevalence rates of DGBI (Rome Foundation Global Epidemiology Study, RFGES), with six independent variables: Helicobacter pylori prevalence and household size as proxy measures for orofecal infections, gross domestic product per capita (GDP), and median age as a proxy measures for socioeconomic development, density of fast food outlets (FFO) per 100,000 population as proxy measure for processed food exposure, and suicide mortality rate per 100,000 people, and world happiness scores were used as a proxy for psychological stress. The data were retrieved from publicly accessible datasets (United Nations, CIA World Factbook, World Bank, World Happiness Report, commercial/financial reports of a global FFO chain). We used linear regression to assess variables in univariate and multivariate analysis and report standardized β coefficients with 95% confidence intervals (CI). The regression model revealed that the overall prevalence of DGBI was inversely associated with both GDP per capita (β = -0.57, 95% CI: -0.92, -0.22, p = 0.002) and happiness scores (β = -0.433 95% CI: 0.821, -0.065, p = 0.023), while being positively associated with H. pylori prevalence (β = 0.40, 95% CI: 0.008, 0.81, p = 0.046). The prevalence of functional constipation (FC) was also inversely associated with GDP per capita (β = -0.50, 95% CI: -0.86, -0.13, p = 0.01) and happiness scores (β = -0.497, 95% CI: -0.863, -0.132, p = 0.01), while being positively associated with H. pylori prevalence (β = 0.53, 95% CI: 0.16, 0.91, p = 0.007). The Multivariate model analysis revealed that combining the factors of H. pylori prevalence, suicide rate, household size and happiness scores showed statistically significant association with FC (p = 0.039). Household size (β = -0.43, 95% CI: -0.82, 0.038, p = 0.033) and suicide rates (β = 0.55, 95% CI: 0.19, 0.90, p = 0.004) were statistically significantly associated with functional diarrhea. Irritable bowel syndrome (IBS) was associated with GDP per capita (β = -0.40, 95% CI: -0.79, -0.014, p = 0.043) and happiness scores (β = -0.390, 95% CI: -0.778, -0.003, p = 0.049). Utilizing publicly available data, the prevalence of DGBI across diverse countries is linked to various socio-cultural and environmental factors. Collectively, the data suggests that the prevalence of DGBI is increased in less prosperous regions of the world.

RELIGIOUS IDENTITY, (SELF-)IDENTIFICATION AND STATISTICS

The article examines the problems of religious statistics due to the multifaceted nature of religious identity and flexibility of religious identification and self-identifi cation. This is the cause of frequent diff erences in the estimated data on the number of followers of a particular confession. Population censuses could be a universal and comparable source, but not all countries ask about religious affiliation in the census. And even when there is a question on religion in the census, differences in wording (and differences in the meaning behind the wording) do not always allow for valid comparisons. Therefore, it is more common to find estimates of the number of followers of denominations based on various surveys. Religious identity is very multidimensional, and it often overlaps with other types of identity: ethnic, civil, local. A person can be associated with a certain faith by accepting the dogmatics, or it can be a set of practices and actions that define a way of life or certain milestones of the life cycle or moments/ periods of the religious calendar. Finally, the very feeling of belonging to cultural and historic tradition can bind a person to a particular faith. Therefore, in order to study quantitative indicators of religiosity, it is more convenient to operate with the procedural concepts of identifi cation and self-identifi cation. Religious statistics, or religious demography, was developed in the 1960s, i.e. in the period when the traditional forms of religiosity were being eroded, when religious dynamics in diff erent regions of the world were becoming more and more different, when it seemed that, at least in the West, religion was losing its position. Religious statistics require the adoption of some form of categorization. This article examines whether classifi cation should always be based on denominational principle alone. Religious statistics is a complex and delicate instrument, but nevertheless it draws some contours of religious structure, reveals the peculiarities of the religious situation and allows us to see the trends of its development.

Mediation of Climate Anxiety in Doctors and Nurses: A Structural Equation Modelling Study

Aims. The goal of this meteorological psychology study is to understand the behavioral implications on the professional lives of medical professionals in one of the most polluted regions of the world with a compromised air quality index. There is a paucity of literature to explore climate ethics as to how behavioral aspects of morals may alter related to climate change. Methods. The study is a cross-sectional correlational quantitative study formulated through purposive sampling. Research took place during the smog season of the world’s most polluted city. The research instruments of Climate anxiety, pain and distress, and cognitive failure were administered to a sample of 211 medical professionals. Confirmatory factor analysis was conducted and resulted in robust model fitness with indices between .80 and .90. Item loadings for three factors were statistically ideal of greater than. 30. Barron and Kenny’s approach was applied to conduct mediation through path analysis in which climate anxiety significantly mediates between pain distress and cognitive failure (β = .51; p < .0001). Multiple regression results using bootstrap confidence showed that climate anxiety (β = .993; p < .0001), and pain distress result in cognitive failure. The independent sample t-test was carried out to explore if nurses and doctors are different in terms of experiencing climate anxiety, pain distress, and cognitive failure. Results. There is a strong significant association among climate anxiety, pain distress, and cognitive failure with strong to moderate correlation values, p < .001. Confirmatory factor analysis was conducted and resulted in robust model fitness with indices between .80 and .90. Item loadings for three factors were statistically ideal at .30. 102 Nurses (M = 85.216; SD = 4.94) were more painfully distressed, prone to cognitive failure, and have climate anxiety as compared to 109 doctors (M = 71.211; SD = 9.23).

Effect of Mild or Moderate Hepatic Impairment on the Pharmacokinetics of Avacopan, a Small-Molecule Complement C5a Receptor Antagonist, for the Treatment of Antineutrophil Cytoplasmic Autoantibody-Associated Vasculitis.

Avacopan is currently approved in several regions of the world as an oral treatment in combination with standard therapy, including glucocorticoids, for adult patients with severe active antineutrophil cytoplasmic autoantibody-associated vasculitis. In vitro and clinical studies have established that avacopan is primarily eliminated through cytochrome P450 3A4 metabolism. This Phase 1, open-label, single-dose study (ClinicalTrials.gov identifier: NCT06004934) was conducted to evaluate the effect of mild (n=8) or moderate (n=8) hepatic impairment compared with normal hepatic function (n=8) on the pharmacokinetics, safety, and tolerability of a single oral dose of 30mg of avacopan in patients without active antineutrophil cytoplasmic autoantibody-associated vasculitis. Relative to participants with normal hepatic function, in participants with mild or moderate hepatic impairment, the avacopan area under the plasma concentration-time curve from time 0 to infinity geometric mean ratios (90% confidence intervals) were 1.3 (0.9-2.0) and 1.1(0.6-2.0), respectively, and the avacopan maximum plasma concentration geometric mean ratios (90% CIs) were 1.0(0.8-1.3) and 0.8 (0.6-1.1), respectively. The geometric mean ratios of metabolite M1 also revealed no pharmacokinetically relevant increase in the peak exposure of M1 in participants with mild or moderate hepatic impairment. Thus, no avacopan dosage adjustment is necessary for patients with mild or moderate hepatic impairment.

N2O Emission Factors from Wastewater Treatment Plants Based on Literature Statistics and Model Fitting

The emission of nitrous oxide （N2O） during wastewater treatment cannot be ignored. The analysis of statistical data from literature based on 126 empirical studies revealed that the geographical factors of wastewater treatment plants （WWTPs） had a significant impact on N2O emission factors. However, the N2O emission factors of WWTPs in all regions of the world were generally lower than the Intergovernmental Panel on Climate Change （IPCC） recommended values. In China, the N2O emission factors （in N2O-N/Ninfluent） of WWTPs were approximately 0.000 35-0.065 20 kg·kg-1. Meanwhile, the N2O emission factors of different wastewater treatment processes were also significantly different, especially since the sequencing batch reactor （SBR） process had higher emissions. The use of uniform default emission factors for accounting was prone to overestimate N2O emissions, and it is recommended that countries conduct actual monitoring or modeling studies to develop categorical emission factors suitable for local conditions. In addition, the N2O emission factor based on total nitrogen （TN） removal was weakly negatively correlated with TN removal in 126 empirical data, which was more in line with bioprocessing stoichiometry and could provide an accurate accounting method for N2O. To this end, a digital twin model was developed to dynamically simulate a case anaerobic-anoxic-aerobic （AAO） WWTP to comprehensively quantify the dynamic emission behavior of N2O, which demonstrated that N2O emissions had significant seasonal and daily variability and were only equivalent to 11% of the calculated value of the emission factor based on the IPCC recommendation. Comparing the scatter linear fitting and categorical mean exponential fitting methods, it was found that the latter could more accurately reflect the negative correlation between the N2O emission factors and the TN removal rate, and an exponential regression equation between the average N2O emission factor based on the amount of TN removed and the TN removal rate was further developed to predict the N2O emission. The dynamic simulation and categorical index fitting methods provided in this study are important references for the accurate accounting of N2O emissions in similar WWTPs and provide help for understanding and responding to the N2O emission problems.

Pharmacological Activities, Phytochemistry and Traditional Uses of Moringa oleifera

Moringa oleifera Lam., more commonly referred to as munga, is a plant that is widely cultivated in India and is used for medicinal purposes. In the family Moringaceae, it is classified as a member. The fact that this plant possesses a multitude of advantageous pharmacological effects, including as anti-diabetic, hepatoprotective, anti-inflammatory, anti-fertility, anti-cancer, anti-microbial, and antioxidant qualities, makes it an appealing nutritional herb. Different names for this species include the horse radish tree and the drumstick tree, to mention only two of them. Each and every part of this plant contains a component that can be utilised for medical purposes. Milk protein, vitamin A, and vitamin C are all found in high concentrations in this food. There are many different chemicals that are considered to be active phytoconstituents. These substances include proteins, quinine, saponins, flavonoids, tannin, steroids, glycosides, fixed oils, and lipids. This plant also has a home in the tropical regions of the world. These are some extra components that are included: both niazinin A and niazinin B, as well as niazimicin A and niaziminin B. In the fight against malnutrition, the plant's high phytonutrient concentration makes it an effective natural integrator that complements other natural remedies. The findings of studies that investigated the phytochemistry of different plant parts have shown that plants contain a large quantity of organic molecules, which include both primary and secondary metabolites. After doing research on the pharmacological and nutraceutical properties of the plant, it has been determined that it can be utilised in the treatment of a variety of ailments. Through this review, the phytochemical composition of the plant, as well as its therapeutic applications and pharmacological effects, are discussed in depth.

Functional characteristics and storage stability of hot air assisted radio frequency treated pearl millet

Nutricereals, pearl millet flour consumption is hardly limited to a few specific regions of the world owing to the development of early rancidity on storage. Hot air assisted radio frequency technology (HARF) was used to improve the storage stability of pearl millet flour. Pearl millets at different moisture levels of 10.5±0.5, 12 & 15% were subjected to HARF for 5-, 10- & 15-min exposure period under fixed electrode position. The results revealed that significant reduction in peroxide value and free fatty acid values of flours from treated pearl millet was found compared to decorticated raw flour; thereby, the storage stability enhancement of pearl millet flour up to 180 days was achieved. No significant difference was found for emulsifying, foaming and cooking properties between treated pearl millet at 15% moisture, 15 min exposure and decorticated raw one. In contrast, the bulk density of flour from treated pearl millet was found higher. Significant increase in hardness of cooked grains after the treatment was observed while the springiness, cohesiveness and gumminess values did not vary. In FTIR analysis, no difference was observed between the peaks of whole and decorticated pearl millet flours in both untreated and treated samples.Graphical

Population structure and genetic diversity of mango (Mangifera indica L.) germplasm resources as revealed by single-nucleotide polymorphism markers.

Mango is a vital horticultural fruit crop, and breeding is an essential strategy to enhance ongoing sustainability. Knowledge regarding population structure and genetic diversity in mango germplasm is essential for crop improvement. A set of 284 mango accessions from different regions of the world were subjected to high-throughput sequencing and specific-locus amplified fragment (SLAF) library construction to generate genomic single-nucleotide polymorphism (SNP). After filtering, raw data containing 539.61 M reads were obtained. A total of 505,300 SLAFs were detected, of which, 205,299 were polymorphic. Finally, 29,136 SNPs were employed to dissect the population structure, genetic relationships, and genetic diversity. The 284 mango accessions were divided into two major groups: one group consisted mainly of mango accessions from Australia, the United States, Cuba, India, Caribbean, Israel, Pakistan, Guinea, Burma, China, and Sri Lanka, which belonged to the Indian type (P1); the other group contained mango accessions from the Philippines, Thailand, Indonesia, Vietnam, Cambodia, Malaysia, and Singapore, which belonged to Southeast Asian type (P2). Genetic diversity, principal component analysis (PCA), and population structure analyses revealed distinct accession clusters. Current results indicated that the proposed hybridization occurred widely between P1 and P2. Most of the accessions (80.99%) were of mixed ancestry, perhaps including multiple hybridization events and regional selection, which merits further investigation.

Registry of patients with autoimmune liver diseases in St. Petersburg: clinical and epidemiological data

BACKGROUND: The problem of autoimmune liver diseases, which include autoimmune hepatitis, primary biliary cholangitis, primary sclerosing cholangitis and their overlap syndromes is an urgent problem of modern medicine. This is associated with an increase in the incidence and prevalence of these nosologies in different regions of the world. Absence of an established etiological factor, prolonged asymptomatic or low-symptomatic course, lack of pathognomonic clinical picture, imperfect diagnostic criteria, insufficient awareness of physicians lead to late diagnosis of autoimmune liver diseases and, accordingly, untimely initiation of treatment, which often reduces its effectiveness, affecting the further prognosis and survival rate for this group of patients. Patients with autoimmune liver diseases require long-term, often lifelong therapy and dispensary observation. Epidemiological registries along with autoimmune liver diseases registries, including various nosologies of autoimmune liver diseases, form an important database on the incidence, clinical features, development and course of the disease, variants of therapy and response to therapy, presence of extrahepatic manifestations as well as allow analysis of all data in dynamics. AIM: To analize data on various nosologies of autoimmune liver diseases, demographics, clinical symptoms, timing of the first symptoms, timing of diagnosis, and the nature of extrahepatic manifestations. MATERIALS AND METHODS: This article presents data from the autoimmune liver diseases Register of the North-West Hepatology Centre (North-West Autoimmune Liver Diseases Register) of the North-Western State Medical University named after I.I. Mechnikov. RESULTS: The study obtained data on the nosological structure, demographic characteristics, timing of diagnosis verification, clinical symptoms and extrahepatic manifestations of autoimmune liver diseases. CONCLUSIONS: Establishing registers of autoimmune liver diseases, providing information about patients with these diseases, will help to assess the effectiveness of examination and treatment methods, factors affecting the course and prognosis of the disease. Analysis of the data obtained will help to develop screening programs, diagnostic algorithms and differential diagnostics of autoimmune liver diseases, which, in turn, will facilitate the diagnostic process, shorten the time of diagnosis, make it possible to start therapy earlier and, consequently, improve the prognosis and survival rate for this group of patients.

Bacterial and fungal microbes associated with cricket rearing systems in the tropics

Abstract House crickets (Acheta domesticus) and field crickets (Gryllus bimaculatus) are among the commonly reared insects for human and animal consumption. However, the potential contamination of rearing facilities with microbes poses a serious threat to sustainability of production of these insects, especially in poorer regions of the world where strategies for ensuring microbe-free environments are lacking in most cases. In this study, bacterial and fungal communities associated with cricket-rearing facility under partially controlled conditions were isolated and examined using morphological and molecular marker-based approaches. BLAST analysis of amplified 16S rRNA sequences revealed similarities to the bacterial genera Rickettsiella, Enterobacter, Bacillus, Levilactobacillus, and Aeromonas, while analysis of the ITS sequences revealed similarities to the fungi Pithomyces spp., Fusarium spp., Aspergillus spp. and Tetrapisispora spp. Increased mortality and reduced nutritional contents of crickets exposed to the bacterial and fungal microbes were evident. Of notable concern is the contamination of the crickets with spore-forming bacteria and toxin-forming bacteria as well as fungi. Although the levels of mycotoxin were relatively low compared to the threshold set by many countries in sub-Saharan Africa, the detection of aflatoxin in both species of crickets is of great concern as any dosage can pose serious health risks to consumers. Strategies to minimize contamination of crickets by entomopathogenic microbes are outlined, and future areas of research are suggested.

Seis-PnSn: A Global Million-Scale Benchmark Data Set of Pn and Sn Seismic Phases for Deep Learning

Abstract The seismic phases Pn and Sn play a crucial role in investigating the velocity and anisotropic characteristics of the uppermost mantle. However, manually annotating these phases can be time-intensive and prone to subjective interpretation. Consequently, the use of travel-time data for these seismic phases remains limited. Despite the potential of deep learning to address this challenge, the scarcity of extensive training data sets for Pn and Sn presents significant constraints. To address this challenge, our research compiled a global million-scale benchmark data set of Pn and Sn seismic phases, namely Seis–PnSn. The data set is derived from earthquake events with epicenter distances ranging from 1.8° to 18°. The high-quality travel-time data used in this study are all from the International Seismological Centre and span the period 2000 to 2019. The waveform data were sourced from data centers located in different regions of the world under the International Federation of Digital Seismograph Networks. By leveraging the unique attributes of this data set, we trained baseline models and explored the prevailing challenges in deep-learning-based Pn and Sn phase picking as the scope transitions from local to regional epicenter distances. Our results show that the performance of the model is considerably enhanced after training on the proposed data set. Our study is a significant complement to the data foundation for future data-driven Pn and Sn seismic phase-picking studies, which will contribute to enhancing our understanding of the uppermost mantle structure of Earth, for example, the seismic velocity, anisotropy, and attenuation characteristics.

Biogeography and community assembly of soil fungi from alpine meadows in southwestern China show the importance of climatic selection

Soil fungi are pivotal in alpine and arctic ecosystems that are vulnerable to climate changes. Previous studies have shown broad connections between soil fungi in the arctic and alpine regions, but most of these studies are mainly from Europe and North America, with more sporadic studies from East Asia. Currently, little is known about the biogeographic relationships between soil fungi in alpine meadows of southwestern China (AMSC) and other regions of the world. In addition, the regional-scale spatial patterns of fungal communities in the AMSC, as well as their driving factors and ecological processes, are also poorly understood. In this study, we collected roots and surrounding soils of two dominant ectomycorrhizal plants, Bistorta vivipara and B. macrophylla from the AMSC, and performed bioinformatic and statistical analyses based on high-throughput sequencing of ITS2 amplicons. We found that: (1) fungi from the AMSC were closely related with those from boreal forests and tundra, and saprotrophic fungi had higher dispersal potential than ectomycorrhizal fungi; (2) community compositions exhibited clear divergences among geographic regions and between root and soil samples; (3) climate was the predominant factor driving regional-scale spatial patterns but had less explanatory power for saprotrophic and total fungi from roots than those from soils; (4) homogeneous selection and drift were the key ecological processes governing community assembly, but in communities of saprotrophic and total fungi from soil samples, drift contributed less and its role was partially replaced by dispersal limitation. This study highlights the importance of climatic selection and stochastic processes on fungal community assembly in alpine regions, and emphasizes the significance of simultaneously investigating fungi with different trophic modes and from both roots and soils.

Comparative High-Throughput Sequencing Analysis of the Bacterial Community Associated with the Rhizosphere of Date Palm (Phoenix dactyllifera L.) Irrigated with Treated Wastewater and Groundwater

Water scarcity is a major agricultural issue in most arid and semi-arid regions of the world. Alternative water supplies, such as the reuse of wastewater for agricultural irrigation, have been introduced. However, little is known about their impact on the soil and rhizosphere microbiomes that receive irrigation. Therefore, this work evaluates the impact of treated wastewater (TWW) irrigation on the soil and rhizosphere bacterial communities of date palms in Al Madinah, Saudi Arabia. In this study, metagenomic DNA from the rhizosphere of the date palm was sequenced using Illumina high-throughput sequencing. According to the observed OTUs, Chao1 richness estimations, and Shannon diversity values, soils from groundwater-irrigated date palms showed higher microbial diversity than did soils from TWW-irrigated date palms. A total of 569 OTUs were generated; most of them (97.3%) were assigned into 15 different phyla, whereas 2.7% were marked as unclassified. DNA sequence analysis of the WWT-irrigated rhizosphere showed that the most abundant phyla were Firmicutes (43.6%), Bacteroidetes (17.3%), Proteobacteria (15.2%), and Actinobacteria (14.6%), representing more than 90.7% of the total community, while the soil of the rhizosphere irrigated with GW was dominated by Actinobacteria (44.1%), Proteobacteria (23.4%), Firmicutes (15.5%), and Gemmatimonadetes (4.9%). The most frequently observed species in the two soils were also different. The dominant species in TWW-irrigated soil was Planococcus plakortidis, which is prevalent in saline and moderately saline habitats and can play an important ecological role. The GW-irrigated rhizosphere exhibited higher levels of biocontrol bacteria, particularly Nocardioides mesophilus. These results provide a comprehensive understanding and insights into the population dynamics and microbiome of date palm rhizosphere. The findings show that the irrigation water quality has a significant impact on the microbiome composition. Identifying the microbial diversity is the first step toward determining the best way to use TWW in irrigation.

Environmental Factors Related to Climate Change Alter the Chemical Composition and Biological Activity of Lavandula viridis L’Hér Essential Oil

Climate change is affecting all regions of the world, and the Mediterranean region is one of the most affected. Plants accumulate secondary metabolites as an adaptive response to stress circumstances. The present study investigated the effect of different abiotic factor conditions (drought, moderate heat, severe heat, salinity, and UV-B radiation) on the essential oil (EO) yield, composition (volatile profile), and biological activity (enzyme inhibition and antioxidant activity) of Lavandula viridis L’Hér. In general, the environmental conditions increased the extraction yield of EO. Eighty-two compounds were identified in the EO and environmental factors induced some quantitative changes in EO composition. Severe heat and salinity conditions increased the concentration of the two most abundant compounds, 1,8-cineole and camphor. Severe heat also increased the potential of EO to inhibit the enzymes butyrylcholinesterase and tyrosinase. Drought, salinity, and UV-B radiation promoted the ability of EO to inhibit acetylcholinesterase. In addition, heat and drought enhanced the antioxidant activity of EO. These results are relevant for exploring the potential of this EO for industrial applications, although future studies combining the factors studied are important to understand the influence of synergistic effects on the composition and bioactivity of the plant products obtained.