This study is based on the theory of spatial structure and uses the geographic information system's (GISs) spatial analysis technology to investigate the spatial distribution characteristics and influencing factors of 243 national night cultural and tourism consumption agglomeration zones (NNCTCAZs) in China. Furthermore, this study employs various analytical methods, including the nearest index, geographic concentration index, imbalance index, nuclear density analysis, buffer analysis and geographic detector method. The results reveal that NNCTCAZs exhibit an imbalanced spatial distribution, with a predominant concentration in the east and southwest regions of China. Furthermore, the ‘core-edge’ structure of this distribution is discernible. The spatial distribution density of NNCTCAZs is uneven, with high-density areas primarily located in the Yangtze River Delta, Pearl River Delta and Sichuan–Chongqing regions. This distribution pattern exhibits the characteristics of being progressive, that is, strong in the east and west and having small agglomerations with large dispersion. In addition, these areas are mainly concentrated in the central regions of cities and the surrounding areas of popular tourist attractions. The spatial layout of NNCTCAZs is mainly influenced by the level of social development, the tourism industry and regional gross domestic product (GDP), which are considered the core determinants. Furthermore, the development level of traffic conditions plays a crucial role in shaping the spatial layout, whereas the impact of the cultural environment and economic conditions is comparatively less pronounced.