Regional Adiposity Research Articles

Association of Hot Tea Consumption with Regional Adiposity Measured by Dual-Energy X-Ray Absorptiometry in NHANES 2003-2006.

This study aimed to investigate the potential antiobesity benefits of hot tea consumption at the population level. Using data from the National Health and Nutrition Examination Survey(NHANES) 2003-2006, the association between hot tea consumption and dual-energy x-ray-measured body fat was examined in a large representative sample of US adults (n = 5,681, 51.9% women). Compared with non-tea drinkers, men who consumed 0.25 to 1 cupperday of hot tea had 1.5% (95% CI: 0.4% to 2.6%) and 1.7% (95% CI: 0.4% to 3.0%) less total and trunk body fat, respectively. The associations were stronger among men 45 to 69 years old compared with younger men (20-44 years). For men who consumed 1 or more cupsperday of hot tea, lower total (-1.2%, 95% CI: -2.3% to -0.2%) and trunk body fat (-1.3%, 95% CI: -2.6 to -0.1%) was observed among men 45 to 69 years old only. In women, those who drank 1 or more cups per day had 1.5% lower (95% CI: -2.7% to -0.3%) trunk body fat compared with non-tea drinkers. Consumption of hot tea might be considered as part of a healthy diet in order to support parameters associated with metabolic health and may be particularly important in older male age groups in supporting reduced central adiposity.

Effect of exercise training on insulin sensitivity, hyperinsulinemia and ectopic fat in black South African women: a randomized controlled trial.

We investigated the effects of a 12-week exercise intervention on insulin sensitivity (SI) and hyperinsulinemia and associated changes in regional and ectopic fat. Healthy, black South African women with obesity (mean age 23 ± 3.5 years) and of isiXhosa ancestry were randomised into a 12-week aerobic and resistance exercise training group (n = 23) and a no exercise group (control, n = 22). Pre and post-intervention testing included assessment of SI, insulin response to glucose (AIRg), insulin secretion rate (ISR), hepatic insulin extraction (FEL) and disposition index (DI) (AIRg × SI) (frequently sampled i.v. glucose tolerance test); fat mass and regional adiposity (dual-energy X-ray absorptiometry); hepatic, pancreatic and skeletal muscle fat content and abdominal s.c. and visceral adipose tissue volumes (MRI). Exercise training increased VO2peak (mean ± s.d.: 24.9 ± 2.42 to 27.6 ± 3.39 mL/kg/min, P < 0.001), SI (2.0 (1.2-2.8) to 2.2 (1.5-3.7) (mU/l)-1 min-1, P = 0.005) and DI (median (interquartile range): 6.1 (3.6-7.1) to 6.5 (5.6-9.2) × 103 arbitrary units, P = 0.028), and decreased gynoid fat mass (18.5 ± 1.7 to 18.2 ± 1.6%, P < 0.001) and body weight (84.1 ± 8.7 to 83.3 ± .9.7 kg, P = 0.038). None of these changes were observed in the control group, but body weight increased (P = 0.030). AIRg, ISR and FEL, VAT, SAT and ectopic fat were unaltered after exercise training. The increase in SI and DI were not associated with changes in regional or ectopic fat. Exercise training increased SI independent from changes in hyperinsulinemia and ectopic fat, suggesting that ectopic fat might not be a principal determinant of insulin resistance in this cohort.

Metastasis and dissection of infrapyloric lymph node in the colon cancer of hepatic flexure

Colon cancer is one of the most common malignancies of the alimentary tract, and one main metastatic route is lymph node metastasis. Thorough dissections of regional lymph nodes is one of the core surgical treatment of right colon cancer. D3 lymphadenectomy and complete mesocolic excision (CME) are generally accepted surgical methods for right colon cancer, which can improve the standardization of surgery, improve the quality of tumor resection, and provide more lymph nodes dissectal. Colon cancer of hepatic flexure is likely to have metastasis of the infrapyloric lymph nodes (No.206), which are not regional lymph nodes. Lymph node dissection of No.206 group belongs to extended right hemicolectomy, which involves many vascular variations and complicated peripheral anatomical structure. The theory of fascial surgery provides surgeons with anatomic basis and a clear understanding of the anatomical structure of the infrapyloric region, which is an important theoretical basis for the thorough dissection of lymph nodes in No.206 group, and can completely remove the mass, regional lymph nodes and adipose connective tissue, so as to achieve the goal of non-bleeding surgery. Lymph nodes in No.206 group were dissected, not just the visible lymph nodes, but the entire lymph nodes and lymphatic vessels in the region, including adipose tissue. Extended right hemicolectomy requires higher surgical techniques. The survival benefits of extended right hemicolectomy are not supported by high-level evidence. It is still controversial whether the infrapyloric lymph node dissection should become routine for colon cancer of hepatic flexure. In this article, the metastasis and dissection of infrapyloric lymph node in colon cancer of hepatic flexure is elucidated.

Associations between body fat distribution and cardiometabolic risk factors in mixed-ancestry South African women and men.

To investigate the relationship between body fat distribution and cardiometabolic risk in mixed-ancestry South African (SA) men and women, and to explore the effect of menopausal status on these relationships in women. In a cross-sectional study, 207 mixed-ancestry SA women and 46 men underwent measurement of body composition using dual-energy X-ray absorptiometry, blood pressure, oral glucose tolerance, lipid profile and high-sensitivity C-reactive protein determination. The associations between different body fat compartments and associated cardiometabolic risk factors were explored. Men had less percentage fat mass ( %FM) [26.5% (25-75th percentiles: 19.9-32.5) vs 44.0%(39.8-48.6), p ≤ 0.001], but more central and less peripheral fat (both p <0.001) than women. Post-menopausal women had greater %FM, waist and visceral adipose tissue (VAT), and less gynoid %FM than pre-menopausal women (all p ≤ 0.004). After adjusting for age and gender, VAT accounted for the greatest variance in insulin resistance (R2 = 0.27), while trunk %FM and leg %FM accounted for the greatest variance in triglyceride (R2 = 0.13) and high-density lipoprotein cholesterol concentrations (R2 = 0.14). The association between fat mass and regional subcutaneous adipose tissue and cardiometabolic risk factors differed by gender and menopausal status. Central fat was the most significant correlate of cardiometabolic risk and lower body fat was associated with reduced risk. These relationships were influenced by gender and menopausal status.

FSH levels are adversely associated with markers of regional adiposity in postmenopausal women

FSH levels are adversely associated with markers of regional adiposity in postmenopausal women

Regional Adipose Compartments Confer Different Cardiometabolic Risk in Children and Adolescents:: The China Child and Adolescent Cardiovascular Health Study

ObjectiveTo investigate the associations of fat mass in specific regions with cardiometabolic risk factors in Chinese children and adolescents. Patients and MethodsThis cross-sectional study consisted of 8460 children and adolescents aged 6 to 18 years from Chinese urban areas who underwent dual-energy x-ray absorptiometry for regional fat masses and had cardiometabolic risk factors measured between January 1, 2013, and December 31, 2015. ResultsIn the multivariate model containing arm fat mass, leg fat mass, and trunk fat mass, after adjustment for region, family income, age, puberty development, physical activity, and smoking, higher trunk fat mass was independently associated with greater odds of clustered cardiometabolic risk (odds ratio [OR], 2.21; 95% CI, 1.80-2.72), higher leg fat mass was associated with lower odds of clustered risk (OR, 0.72; 95% CI, 0.59-0.87), and arm fat mass was not significantly associated with clustered risk (OR, 1.22; 95% CI, 0.97-1.55). In the multivariate model including android fat mass, gynoid fat mass, and other covariates, higher android fat mass was positively associated with clustered risk (OR, 2.23; 95% CI, 1.88-2.64), but gynoid fat mass was not associated with clustered risk (OR, 0.86; 95% CI, 0.72-1.03). Analyses for individual risk factors demonstrated similar results. ConclusionFat stored in different regions has differential influences on cardiometabolic risk in youth. Prospective studies are warranted to evaluate the long-term impacts of regional fat masses in childhood on cardiovascular risk in adulthood.

The cresty neck score is an independent predictor of insulin dysregulation in ponies.

Generalized obesity, regional adiposity, hyperinsulinemia and hypertriglyceridemia are all potential indicators of equine metabolic syndrome (EMS). This study aimed to assess the relationship between morphometric measurements of body condition and metabolic hormone concentrations in ponies, with and without a neck crest or generalised obesity. Twenty-six ponies were assigned a body condition score (BCS) and cresty neck score (CNS). Height, girth, and neck measurements were taken. An oral glucose test (OGT; 0.75g dextrose/kg BW) was performed and blood samples collected prior to and 2 hours post dosing. Basal blood samples were analysed for blood glucose, serum insulin, triglyceride and leptin, and plasma HMW adiponectin concentrations. Post-prandial samples were analysed for serum insulin concentration. The ponies were grouped as having a) a normal to fleshy body status (BCS ≤7 and CNS ≤2; n = 10); b) having a high CNS, but without generalised obesity (BCS ≤7 and CNS ≥3; n = 11), or c) being obese (BCS ≥8 and CNS ≥1; n = 5). Responses to the OGT indicated that both normal and insulin-dysregulated ponies were included in the cohort. Post-prandial serum insulin was positively associated with CNS (P<0.035) and ponies with a CNS ≥ 3 had 5 times greater odds of being insulin-dysregulated. The high CNS group had a greater insulin response to the OGT than those in the normal/fleshy group (P = 0.006), whereas obese ponies did not differ from the other two groups. Basal HMW adiponectin was negatively correlated with post-prandial insulin concentrations (r = -0.5, P = 0.009), as well as being decreased in the group with a high CNS, compared to the obese group (P = 0.05). Cresty neck score was more predictive of insulin dysregulation than BCS, and this may be relevant to the diagnosis of EMS. Adiponectin may also be a measure of insulin dysregulation that is independent of body condition.

Bone morphogenetic protein 2 is a depot-specific regulator of human adipogenesis

BackgroundBone morphogenetic proteins (BMPs) regulate adipogenesis but it is not clear whether they influence regional adipose tissue (AT) development in humans.ObjectiveTo characterise BMP2 expression, BMP2-SMAD1/5/8 signalling, and BMP2′s potential effect on proliferation and adipogenesis in human subcutaneous abdominal and gluteal AT and its constituent preadipocytes.MethodsBMP2 expression was measured in whole AT and immortalised preadipocytes via qPCR and Western blot; secreted/circulating BMP2 was measured by ELISA. The effect of BMP2 on preadipocyte proliferation was evaluated using a fluorescent assay. BMP2′s effect on adipogenesis in immortalised preadipocytes was determined via qPCR of adipogenic markers and cellular triacylglycerol (TAG) accumulation. BMP2-SMAD1/5/8 signalling was assessed in immortalised preadipocytes via Western blot and qPCR of ID1 expression.ResultsBMP2 was expressed and released by abdominal and gluteal AT and preadipocytes. Exogenous BMP2 dose dependently promoted adipogenesis in abdominal preadipocytes only; 50 ng/ml BMP2 increased PPARG2 expression (10-fold compared to vehicle, p < 0.001) and TAG accumulation (3-fold compared to vehicle; p < 0.001). BMP2 stimulated SMAD1/5/8 phosphorylation and ID1 expression in abdominal and gluteal preadipocytes but this was blocked by 500 nM K02288, a type 1 BMP receptor inhibitor (p < 0.001). Co-administration of 500 nM K02288 also inhibited the pro-adipogenic effect of 50 ng/ml BMP2 in abdominal cells; >90% inhibition of TAG accumulation (p < 0.001) and ~50% inhibition of PPARG2 expression (p < 0.001). The endogenous iron regulator erythroferrone reduced BMP2-SMAD1/5/8 signalling by ~30% specifically in subcutaneous abdominal preadipocytes (p < 0.01), suggesting it plays a role in restricting the expansion of the body’s largest AT depot during energy deficiency. Additionally, a waist-hip ratio-increasing common polymorphism near BMP2 is an eQTL associated with ~15% lower BMP2 expression in abdominal and gluteal AT (p < 0.05) as well as altered adipocyte size in male abdominal AT (p < 0.05).ConclusionsThese data implicate BMP2-SMAD1/5/8 signalling in depot-specific preadipocyte development and abdominal AT expansion in humans.

The proposed systemic thermogenic metabolites succinate and 12,13-diHOME are inversely associated with adiposity and related metabolic traits: evidence from a large human cross-sectional study

Aims/hypothesisCirculating succinate and 12,13-dihydroxy-9Z-octadecenoic acid (12,13-diHOME) were recently shown to promote brown adipocyte thermogenesis and protect against metabolic disorders in rodents. This study aimed to evaluate the associations between plasma levels of these metabolites and adiposity and metabolic profile in humans.MethodsFasting plasma succinate and 12,13-diHOME levels were quantified using ultra HPLC-tandem MS in 2248 individuals (50% female, mean age 41.3 ± 5.9 years, mean BMI 26.1 ± 4.6 kg/m2) in addition to fasting plasma biochemistry. Total and regional adiposity were assessed with dual-energy x-ray absorptiometry. An age- and sex-adjusted linear regression model was used to determine the associations between succinate and 12,13-diHOME levels and body composition and metabolic profile. Two-sample Mendelian randomisation was used to assess the associations between genetically determined BMI and metabolic traits with circulating plasma succinate and 12,13-diHOME.ResultsA one-SD higher plasma succinate and 12,13-diHOME concentration was associated with a 0.15 SD (95% CI 0.28, 0.03) and 0.08 SD (0.15, 0.01) lower total fat mass respectively. Additionally, a one-SD higher plasma 12,13-diHOME level was associated with a 0.09 SD (0.16, 0.02) lower fasting plasma insulin and 0.10 SD (0.17, 0.04) lower plasma triacylglycerol. In Mendelian randomisation analyses, genetically determined higher BMI, fasting hyperinsulinaemia and elevated lipid levels were not associated with changes in either plasma succinate or plasma 12,13-diHOME concentrations. No indications of bias due to directional pleiotropy were detected in the Mendelian randomisation analyses.Conclusions/interpretationOur findings tentatively suggest that plasma succinate and 12,13-diHOME may play a role in the regulation of energy metabolism and brown adipose tissue activation in humans. Further studies encompassing direct assessment of brown adipose tissue activity and dietary supplementation are necessary to investigate the potential beneficial effects of these metabolites on systemic metabolism.

Accuracy of global and/or regional anthropometric measurements of adiposity in screening sleep apnea: the ELSA-Brasil cohort

ObjectiveAdiposity is a well-established risk factor for obstructive sleep apnea (OSA) but the existence of a preferable anthropometric measurement is not established or whether the combination of measurements may improve the accuracy to detect OSA. This study aimed to compare the accuracies of body mass index (BMI), several surrogate markers of body fat (in isolation or combined) and validated questionnaires for screening OSA. MethodsA total of 2059 participants from the ELSA-Brasil study given anthropometric measurements using standard procedures and a home sleep study. OSA was defined by an apnea-hypopnea index ≥15 events/hour. ResultsThe frequency of OSA was 32.3%. Compared with the non-OSA group, all anthropometric measurements were higher in the OSA group. Age and gender-adjusted BMI afforded the highest accuracy to detect OSA [AUC = 0.760 (0.739–0.781)], followed by waist [AUC = 0.753 (0.732–0.775)] and neck [AUC = 0.733 (0.711–0.755)] circumferences, waist-to-hip ratio [AUC = 0.722 (0.699–0.745)] and body shape index [AUC = 0.680 (0.656–0.704)]. The combination of two or more anthropometric measurements did not improve the accuracy of BMI in predicting OSA. The adjusted BMI had similar predictive performance to the NoSAS score [AUC = 0.748 (0.727–0.770)] but a better accuracy than the Berlin Questionnaire [AUC = 0.676 (0.653–0.699)]. ConclusionsDespite one's intuition, surrogate markers of regional adiposity are not better than BMI in screening OSA. Combining measurements of global and/or regional adiposity did not have additional value in detecting OSA. The merely fair accuracy range of BMI and sleep questionnaires underscore the need for additional tools to improve OSA underdiagnosis.

2103-P: Sex Differences in Human Adipose Tissue: Comprehensive Transcriptome Profiling

The sexual dimorphism of adipose tissue is incompletely understood. Differences in total and regional adiposity have been described, but the molecular basis for these differences is unclear. We have conducted the first comprehensive transcriptome analysis in adipose tissue from healthy age and BMI-matched women and men. Results demonstrate upregulation of lipid and carbohydrate metabolism in pre and postmenopausal women vs. men. In men few genes were upregulated: the majority of these were related to inflammation vs. upregulation of inflammatory genes in men. Measurement of regional and intrahepatic fat, adipose cell size, and systemic insulin resistance complement RNAseq (n=39) and rtPCR (n=54) results. Disclosure G. Bogu: None. M. Snyder: Advisory Panel; Self; Jungla. Consultant; Self; Merck &amp; Co., Inc. Other Relationship; Self; 10x Genomics, Akna, Filtricine, Illumina, Inc., January, Personalis, Qbio, SensOmics. T. McLaughlin: Advisory Panel; Self; January. Stock/Shareholder; Self; Eiger BioPharmaceuticals. Funding American Diabetes Association (1-11-CT-35 to T.M.); National Institute of Diabetes and Digestive and Kidney Diseases

2060-P: Serum Wnt Inhibitor DKK1 Is Associated with Adiposity and Insulin Resistance in Nondiabetic North Americans

Background: The Wnt pathway is known to influence body composition during early human development by regulating bone formation, myogenesis and adipogenesis. We have recently shown that DKK1 is related to measures of adiposity in nondiabetic African ancestry males. Whether these relationships are true across sexes and/or in other human populations remains unknown. In this study we evaluated the relationship of DKK1 with adiposity in a sample of male and female, mixed race, nondiabetic North American individuals. Methods: Fasting serum DKK1 levels were measured using ELISAs in 201 nondiabetic subjects (age range 30.7-60 years, BMI range 19.9-64.0 kg/m2) recruited from the general population via television advertisement and mass mailing. The subjects were predominantly female (85%) and Caucasian (63%). Anthropometrics were obtained and markers of inflammation and glucose metabolism were measured from fasting serum samples. Spearman and partial Spearman correlation analysis was used to determine the association of DKK1 with measures of adiposity and insulin resistance. Results: Circulating DKK1 was positively associated with BMI (r=0.30, p&amp;lt;0.001) and waist circumference (r=0.25, p&amp;lt;0.001), independent of age, race and sex. In addition, DKK1 was positively associated with fasting serum insulin level (r=0.18, p&amp;lt;0.01) and HOMA-IR (r=0.19, p&amp;lt;0.01) in age, sex and race adjusted models. However, these associations were no longer significant after adjustment for BMI. Conclusion: This is the first study looking at correlation of DKK1 with adiposity in North American nondiabetic individuals. Our findings suggest that the link between DKK1 and insulin resistance maybe mediated indirectly through total and regional adiposity and that DKK1 could be a potential early biomarker for diabetes in high risk individuals. These findings support our findings in African ancestry males and add to the evidence favoring potential role of Wnt modulators in adiposity regulation in humans. Disclosure H. Ali: None. E. Oczypok: None. I. Miljkovic: None. R. Cvejkus: None. J.M. Zmuda: None. J. DeLany: None. E.E. Kershaw: Research Support; Self; Regeneron Pharmaceuticals. Funding National Institutes of Health (T32DK007252); Endocrine Fellows Foundation

733-P: Circulating Vaspin Level Was Changed with Endothelial Function, but Not with Visceral or Ectopic Fat during Lifestyle Modification in Korean Type 2 Diabetes

Vaspin is an adipocytokine identified in visceral adipose tissues and has anti-inflammatory effects in vascular smooth muscle cells. We investigated the association between visceral or ectopic adiposity, insulin resistance, inflammatory markers and endothelial function including circulating vaspin, and the effects of lifestyle modification in Korean type 2 diabetes. Total 170 overweight women with type 2 diabetes (age 55.0±7.6 years; BMI 27.4±2.8 kg/m2) were randomly assigned to control (C), diet (D), aerobic exercise (AE) and resistance exercise (RE) and 82 completed the weight loss program for 12 weeks. We assessed BMI, total abdominal (TAF), visceral fat (VAF), liver and spleen attenuation ratio (LSR), and intramuscular adipose tissue (IMAT) at midthigh level using computed tomography, and FMD (Flow-mediated dilation), vaspin, adiponectin/leptin ratio (ALR), free fatty acid (FFA), hsCRP, and insulin sensitivity by homeostatic model assessment insulin resistance (HOMA-IR) at baseline, and 12 week. The log transformed variables are prefixed by 'ln' BMI decreased by 7.07% in RE, 6.42% in D and 3.28% in AE (p&amp;lt;0.001 vs. control). TAF and VAF also decreased by 18.82% and 22.06% in RE, 11.96% and 14.12% in D, 8.30%and 8.38% in D (consecutively, p&amp;lt;0.001 vs. control). ALR was increased comparably in 3 intervention groups. Percent changes (pc) of vaspin, FFA, hsCRP, HOMA IR, FMD, IMAT, LSR were not different among 4 groups. Within group analysis, vaspin was increased from 0.74 to 1.04 (p=0.096) in AE, FMD was increased in D and AE (p&amp;lt;0.001, P=0.035). Crushed the groups, ln-vaspin pc was changed with ln-FMD pc (r=-0.246, p=0.016), but not with pc of ln-VAF, IMAT. Total fat was changed with ALR (r=-0.151, p=0.042). LSR with hsCRP (r=-0.217, p=0.038). These findings suggested that the circulating vaspin level was more related with vascular function rather than regional or ectopic adiposity during lifestyle modification in type 2 diabetes. Disclosure K. Han: None. K. Min: None.

Association between Habitual Coffee Consumption and Indices of Body Fat

BackgroundDespite strenuous public health efforts, obesity rates continue to rise globally, and contribute importantly to increased cardiometabolic morbidity and mortality. Dietary habits play a key role in the development and progression of obesity, and are the target of choice in preventative and therapeutic programs. Although preclinical studies have shown that caffeine may exert anti‐adipogenic effects, in humans the implications of coffee consumption on risks of excess adiposity are unclear.ObjectiveThe objective of this study was to investigate the association between habitual coffee consumption and indices of total and regional body fat in healthy adults.MethodsSeventy‐eight subjects (46 males, mean age 27.3±6.1 years) who took part in research studies conducted at the Mayo Clinic Cardiovascular/Sleep Laboratory and underwent body composition assessment were selected. Absence of cardiovascular or metabolic diseases was ascertained by physical exam and review of medical charts. Height, weight, waist and hip circumference measures were collected for computation of body mass index (BMI, kg/m2) and waist‐to‐hip ratio (WHR). Measurements of total body fat percentage, upper body fat (kg), and lower body fat (kg) were obtained from dual‐energy X‐ray absorptiometry (DXA). Abdominal computed tomography (CT) was used to quantify subcutaneous adipose tissue (SAT, cm2) and visceral adipose tissue (VAT, cm2). Habitual coffee consumption (cups/day; range 0–3) was self‐reported and coded as a binary variable (yes/no).ResultsSixty percent of the subjects (n=47) reported habitual coffee consumption. Age and gender distribution were comparable between groups (P=0.52 and P=0.73, respectively). There was no significant difference between those who reported consuming coffee and those who did not in terms of BMI (23.6±2.7 kg/m2 vs 24±2.8 kg/m2, P=0.56) and WHR (0.85±0.08 vs 0.85±0.07, P=0.7). Likewise, body composition and fat distribution indices were similar between groups: total body fat percentage (coffee drinkers vs non‐coffee drinkers: 30.5±7.2% vs 28.6±7.8%, P=0.27), upper body fat mass (6.6±2.4 kg vs 6.6±3.1 kg, P=0.99), lower body fat mass (9.4±5.2 kg vs 8.4±4.1 kg, P=0.39), SAT (143.4±61.3 cm2 vs 135.9±63.7 cm2, P=0.6), and VAT (57.7±35.5 cm2 vs 70.3±44 cm2, P=0.22). Analysis stratified by gender did not reveal any differential effect of regular coffee consumption on body fat (all Ps&gt;0.26).ConclusionIn healthy, young individuals moderate habitual coffee consumption is not associated with measures of total or regional adiposity. Whether the anti‐obesity effects of caffeine emerge only when it is consumed in larger quantities remains to be determined.Support or Funding InformationResearch support: NC is supported by American Heart Association grant 16SDG27250156 and Mayo Clinic Center for Clinical and Translational Science grant Marie Ingalls Cardiovascular Research Career Development Fund.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

Investigation of the effects of cold stress on angiogenic factors in adipose tissue of obese rats

Adipose tissue produces both vascular growth factors and inhibitors. Since obesity is associated with expansion of the capillary bed in regional adipose depots the balance between these factors may favor angiogenesis. White and brown adipose tissues (WAT and BAT) plasticity, namely the ability to expand, plays a critical role in the regulation of body energy balance. as a thermogenic organ, the activity of BAT counteracts the obesity. BAT possesses extraordinarily complex vascular networks to support its high thermogenic activity. It also meets the needs of supplying oxygen and substrates to fuel thermogenesis and distributing heat throughout the body rapidly. Brown adipose tissue is related to thermogenesis while white adipose tissue is the shape of storage of high‐calorie foods. The temperature conditions, when normal and above, cause a decrease in the activity of brown adipose tissue which plays a role in the regulation of body temperature while the nutrients are collected as adipocytes instead of being consumed as heat. Therefore, in this study, we aimed to compare the differences in angiogenic/antiangiogenic factor levels in normal and obese rats due to cold application and to investigate possible changes in brown adipose tissue due to the application of cold stress. Vascular endothelial growth factor (VEGF), matrix metalloproteinase II (MMP‐II), hypoxia inducible factor 1α (HIF1‐α), angiostatin ve endostatin concentrations were measured in brown adipose tissue (BAT) and white adipose tissue (WAT) from 12 control (6 rats kept in room temperature, 6 rats kept in 10 °C for 72 h) and 12 obese (6 rats kept in room temperature and 6 rats kept in 10 °C for 72 h). All animals were 6 months old. In our the study, vascular endothelial growth factor (VEGF), matrix metalloproteinase‐II, hypoxia inducible factor 1α, angiostatin and endostatin levels were investigated. While angiogenic factors increased in cold stress application in control animals, antiangiogenic factors decreased. Angiogenic factors decreased due to cold stress application and increased in antiangiogenic factors in obese animals. Antiangiogenic factors were found to be increased in brown adipose tissue and angiogenic factors increased in white adipose tissue. It was observed that the increase in antiangiogenic factors was more evident in brown fat tissue of obese rats exposed to cold stress. In addition to nutritional conditions, it is concluded that the temperature conditions of the body are effective on angiogenic/antiangiogenic factors and may be effective on brown fat tissue which plays a role in thermogenesis. It should be evaulated that increased levels of antiangiogenic factors are in obese rats may contribute to reducing adipose tissue activity in cope with obesity.Support or Funding InformationThis research was funded by Inonu University, Department of Scientific Research Projects Project No: FYL‐2018‐943).This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

Parsing the craniofacial phenotype: effect of weight change in an obstructive sleep apnoea population.

Craniofacial structure is an important risk factor in the development of obstructive sleep apnoea. Most craniofacial imaging methods are not feasible for large-scale studies or the clinic. Craniofacial photography is a high-throughput technique for facial phenotyping; however, derived measurements are a composite of skeletal and soft tissue craniofacial information. Weight change is a paradigm to help determine which facial measurements most relate to regional soft tissue (i.e. change with weight) versus skeletal structure (i.e. stable with weight changes). We aimed to assess the association between weight change and changes in key facial measurements from facial photography. Calibrated frontal and profile photographs were taken of participants in weight loss studies (N = 106). Univariate linear regression was used to assess whether weight change explained changes in facial dimensions. Patients lost 11.7 ± 10.8kg body weight and 2.0 ± 2.0cm of neck circumference. Weight changes influenced face width (r = 0.3, p < 0.001), mandibular width (r = 0.4, p < 0.001) and cervicomental angle (r = 0.3, p = 0.001). Facial angles, facial heights and mandibular length were not influenced by weight change. A weight loss paradigm suggests that face and mandibular width and cervicomental angle most strongly reflect regional adiposity. Facial angles and heights are insensitive to weight change and could be more representative of craniofacial skeletal structure. This study informs the interpretation of facial phenotype assessed by this craniofacial photographic method which can be applied to future studies of craniofacial phenotype in OSA.

Evolution of total body and regional adiposity from late adolescence to early adulthood in a birth cohort study

BackgroundDifferences in total body and regional adiposity according to sex are observed from an early age, but these differences become more evident after puberty due to hormonal changes. We aimed to assess the evolution of total body and regional adiposity from 18 to 22 years of age and the associated sociodemographic and nutritional characteristics.MethodsIn total, 3274 individuals from the 1993 Pelotas birth cohort study followed up at 18 and 22 years of age. Measures of total body and regional adiposity were assessed using whole-body dual-energy X-ray absorptiometry (DXA) and the TC2 Three-Dimensional Photonic Scanner. We used fat mass index obtained from DXA as a measure of total body adiposity, and android and gynoid fat mass percentages (android or gynoid fat mass [kg]/total fat mass [kg])*100) as measures of regional adiposity. In addition, waist, hip and thigh circumferences from the photonic scanner were also used as measures of regional adiposity. We evaluated these measurements at 18 and 22 years of age by sex and estimated differences between them according to sociodemographic and nutritional characteristics.ResultsWhile men and women did not differ in terms of BMI, females exhibited a higher fat mass index, gynoid fat mass percentage, and hip and thigh circumferences; men exhibited higher android fat mass percentage and waist circumference at both time points. Increases in all body measurements from age 18 to 22 were observed in men and women, except for gynoid fat mass percentage, which decreased in both sexes. Socioeconomic position and race were the independent variables most associated with adiposity rising from age 18 to 22 in women, with black women and women of lower socioeconomic positions exhibiting larger increases in adiposity.ConclusionThere was an increase in adiposity and a centralization of body shape from late adolescence to early adulthood, indicating possible early risks for noncommunicable diseases in this cohort.

Abstract P255: Central Fat Distribution and Metabolic Health in Normoglycemic Young Women

Purpose: The purpose of this pilot study is to test the hypothesis that fat distribution, rather than overall obesity, is strongly related to insulin sensitivity in young, normoglycemic women. Methods: Thirty-eight non-diabetic young women completed the study (ages 20.7 ±2.8 years; BMI 27.6 ±3.6; 44% Caucasian (17 of 38). Matsuda Insulin Sensitivity Index was calculated from plasma glucose and insulin at 0, 30, 60, and 90 minutes after ingestion of 75g glucose, using established formula, to estimate whole body insulin sensitivity. Whole body and regional adipose tissue was assessed via DXA, abdominal ultrasound of intra-abdominal and pre-peritoneal adipose depots, circumference measures, and skinfold measures. ANOVA was used to assess mean differences among tertiles of Matsuda Index. Partial correlations were controlled for age and race, then additionally controlled for DXA percent body fat to assess associations of fat distribution independent of overall obesity. Results: ANOVA revealed a higher waist circumference (83.8 ±7.8cm vs. 75.6 ±7.2), waist/hip ratio (0.777 ±0.48 vs. 0.716 ±0.05 ), waist/height ratio (0.524 ±0.028 vs. 0.468 ±0.043), ultrasound intra-abdominal thickness (3.57 ±1.3 vs. 2.48 ±1.1) in the least insulin-sensitive tertile, when compared to the most insulin-sensitive tertile, while age, BMI, and other measures of adiposity were comparable among the three groups. Partial correlations controlled for age and race revealed strong negative relationships between Matsuda Index and measures of upper-body and central adiposity, including waist circumference ( r =-.416, p &lt;0.05), waist/hip ratio ( r = -0.500, p &lt;0.01), waist/height ratio ( r =-0.431, p &lt;0.05), IAAT ( r = -0.412, p &lt;0.05), PPAT ( r =-0.381, p &lt;0.05), but not BMI ( r = -0.173, p =ns) or overall percent fat ( r =-0.132 , p =ns). When further controlled for percent body fat, correlations persisted between Matsuda and waist circumference ( r =-.433, p &lt;0.05), waist/hip ratio ( r =-0.488, p &lt;0.01), waist/height ratio (r= -.470, p &lt;0.01), intra-abdominal fat thickness ( r =-0.394, p &lt;0.05) and pre-peritoneal fat thickness ( r =-0.361, p =0.05). Conclusions: Central and upper-body fat distribution, independent of overall obesity, is strongly associated with metabolic health in young non-diabetic women. These data underscore the clinical utility of central adiposity as an marker of metabolic health.

Influence of Sex on the Changes in Regional Fat and Skeletal Muscle Mass in Response to Exercise Training in Adolescents with Obesity.

We retrospectively examined the sex differences in the changes in (1) total fat, total and regional subcutaneous adipose tissue (SAT), visceral fat, and intermuscular fat and (2) total and regional skeletal muscle distribution in response to aerobic exercise (AE) or resistance exercise (RE) in adolescents with obesity. Twenty-eight boys and 27 girls with obesity (BMI ≥95th percentile, 12-18 years) were randomly assigned to 3-month interventions (180 minutes per week) of AE or RE. Changes in total and regional fat and skeletal muscle distribution were assessed by a whole-body magnetic resonance imaging. After controlling for corresponding baseline values, age, and race, changes in body weight, BMI, BMI z-score, and waist circumference were similar between exercise groups (p > 0.05) and sexes (p > 0.05). There were no sex or exercise group differences in the reductions in total fat, total SAT, visceral fat, or intermuscular fat. With AE, boys had greater (p < 0.05) reductions in abdominal SAT as compared with girls. With RE, boys had greater (p < 0.05) increases in total, upper body, and abdominal skeletal muscle as compared with girls. Independent of exercise modality, the improvement in VO2max was greater (p < 0.05) in boys than in girls. Independent of sex, the increase in muscular strength index was higher (p < 0.05) in the RE vs. AE group. With the exception of abdominal SAT, there were no sex or exercise treatment differences in the reductions in total and regional fat. In response to RE, the increases in total and regional skeletal muscle were significantly greater in boys than in girls.

Thigh and abdominal adipose tissue depot associations with testosterone levels in postmenopausal females

Research findings on the relationship between serum androgens and adipose tissue in older females are inconsistent. We aimed to clarify the relationship using state-of-the-art techniques to evaluate associations between body fat distribution and plasma testosterone (T) levels in older postmenopausal women. Observational, cross-sectional study of healthy, community dwelling postmenopausal women. Postmenopausal women (60-80years old) were included in this study. Overall body composition was evaluated by dual-energy X-ray absorptiometry. Abdominal and thigh fat depots were measured by magnetic resonance imaging. Circulating T concentrations were analysed by liquid chromatography-tandem mass spectrometry. Thirty-five women (66.6±0.8years) participated in this study. T levels were positively associated with clinical proxy measures of adiposity including weight (ρ=0.39), BMI (ρ=0.43) and waist circumference (ρ=0.39) (all P<0.05). Fat mass and % body fat were correlated with T levels (ρ=0.42 and 0.38 respectively, both P<0.05). T correlated with overall and superficial abdominal fat (ρ=0.34 and 0.37 respectively, both P<0.05) but not with visceral adipose tissue. T increased with greater thigh fat (ρ=0.49, P<0.05) in both superficial and deep depots (ρ=0.50 and 0.35 respectively, both P<0.05). Our results suggest that postmenopausal women with higher circulating T levels have both higher regional and overall body adiposity. These findings underscore the sexual dimorphism in the relationship between serum androgen levels and adiposity.