The fatigue behavior and strengthening mechanisms of the laser powder bed fusion pure tantalum (LPBF-Ta), coupled with a fatigue life prediction model, were originally explored. The fatigue strength of LPBF-Ta fabricated at the optimal laser energy density (154.16 J/mm3) is 348 MPa (106 cycles), showing a remarkable improvement of about 80 % and 50 % compared with that of the annealed and cold worked commercially pure tantalum, respectively. The microstructural analysis identifies that cellular structures averaging 300 nm in size contribute 438.2 MPa to strengthening, serving as the main contributor to fatigue strength. The breaching of cell walls by dislocations, along with the activation of cross-slip, ensures plasticity and ultimately enhances fatigue strength. Besides, the analysis of the defects reveals that the internal lack of fusion pore can induce the secondary fatigue crack, resulting in a region of brittle crack propagation. Furthermore, a finite life prediction model is established by originally incorporating the size of the crack source defect, and it significantly improves the prediction accuracy of the fatigue life. These results provide guidance for the development of fatigue strengthening strategies for LPBF-Ta.