Addressing the significant challenge of efficiently separating 1,4-dioxane from aquatic environments using traditional adsorbents is crucial. In this study, we evaluated the adsorption performance of MCM-22 and Beta zeolites. Both were identified as highly effective adsorbents. Equilibrium adsorption of 1,4-dioxane was achieved within 20 min for an initial concentration of 500 mg L-1 on both MCM-22 and Beta zeolites. At 288 K, the maximum adsorption capacities fitted by Langmuir isotherm were 79.66 mg g−1 for MCM-22 and 121.66 mg g−1 for Beta zeolite. Fixed-bed column studies demonstrated that the Yan model properly predicted the behavior of both zeolites under varying flow rates. Additionally, MCM-22 exhibited a wider pore size distribution compared to Beta zeolite, while Beta zeolite demonstrated a higher volume of ultra-micropores (<1 nm) than MCM-22. This highlights the importance of pore volume filling in facilitating excellent adsorption of 1,4-dioxane by both zeolites. Further analysis via FTIR and 1H NMR confirmed the significance of Brønsted acid sites in 1,4-dioxane adsorption. The adsorption performance of both zeolites remained unaffected by initial pH values ranging from 2 to 11, as well as variations in ionic strength or the presence of natural organic matter (NOM) for both zeolites. However, the co-existence of 1,1-dichloroethene (1,1-DCE) resulted in competitive adsorption with 1,4-dioxane, leading to a notable decrease in adsorption capacity of 1,4-dioxane. In contrast, the presence of metal ions Cu2+ and Ag+ did not compete for adsorption sites with 1,4-dioxane. Our study demonstrated the effectiveness of the bio-zeolite system in regenerating spent adsorbents, surpassing water and solvent regeneration methods in efficiency and recyclability. Although addressing the persistence of 1,1-DCE remains a challenge, bio-zeolites emerge as versatile tools for mitigating the bio-inhibition of heavy metals, such as Cu2+ and Ag+, while offering a sustainable solution for 1,4-dioxane bioremediation.