Abstract Background Selection of the stable normalizing genes is critical for quantification of gene expression. Unfortunately, till date, no report on systematic assessment of reference genes is known for dromedary camel (Camelus dromedaries). Therefore, this is the first study to validate the stability of five potential reference genes in different tissues of Camel. Results The expression levels of the glyceraldehyde-3P-dehydrogenase (GAPDH), TUBB (β-tubulin), H-Ras protein, c-Myc and ACTB (β-actin) transcripts were quantified in the liver, brain, heart, spleen, kidney, adipose tissue, and skeletal muscle. Based on the stability ranking, performed with the geNorm VBA applet for Microsoft Excel, Normfinder and ΔCt method, the examined genes were arranged as follows GAPDH > ACTB > TUBB >H-Ras > c-Myc. Conclusion Our findings suggest that the GAPDH and ACTB genes can be used as stable reference genes for accurate normalization of gene expression data using qRT-PCR in the Camelus dromedarius.