The aim of this study was to examine the impact of backpack load carriage on lower limb strength and loading rate change in a cohort that match military recruit profiles. Twenty-six participants walked on a treadmill either carrying a military load carriage system (32 kg) or unloaded for 2 h on two consecutive days. Participants ground reaction forces and strength measures were assessed using a force platform and dynamometry, respectively. Testing included assessments before and after treadmill walking on days one and two, and 24 h following day 2. When assessed by mixed methods ANOVA (alpha: 0.05) statistically significant interaction effects were observed for loading peak (p = 0.031), loading rate (p = 0.035) and plantarflexor torque dynamometry variables at 60°s−1 (p = 0.011) and 120°s−1 (p = 0.024). Repeated measures correlation highlighted associations between plantarflexor torque at 60°s−1 and loading rate (r = −0.901, p < 0.001). Load carriage reduced lower limb torque which did not recover between days. Plantarflexor torque reductions were associated with increases in loading rate. Practitioners should consider that load bearers are more likely to experience lower limb injury during multi-day load carriage. Future work should develop protocols to reduce plantarflexor torque loss in order to reduce ground reaction force change.