Reduced Swimming Speed Research Articles

Swimming restricted foraging behavior of two zooplanktivorous fishes Pseudorasbora parva and Rasbora daniconius (Cyprinidae) in a simulated structured environment

In littoral zones of aquatic systems, submerged macrophytes have marked structural variation that can modify the foraging activity of planktivores. Swimming and feeding behavior of Pseudorasbora parva and Rasbora daniconius (Cyprinidae) on their prey Daphnia pulex and Artemia salina, respectively, was studied in a series of laboratory experiments with varying stem densities. A range of stem densities was tested for each of the two species to compare the effect of simulated macrophytes on prey attack rates and swimming speed, average stem distance (D) was measured in fish body lengths for each of the two fish species. We found that, with reducing average stem distance, the attack rate decreased in the similar trend and this trend was similar for both fish species. However, the species differed in the degree to which swimming activity was hindered at increased stem densities, and this was due to species-specific differences in the distance moved with one tail beat. Therefore, we conclude that the reductions in swimming speed with reduced average stem distance are due to the differences in fish movement per tail beat.

TRITURUS NEWTS DEFY THE RUNNING-SWIMMING DILEMMA

Conflicts between structural requirements for carrying out different ecologically relevant functions may result in a compromise phenotype that maximizes neither function. Identifying and evaluating functional trade‐offs may therefore aid in understanding the evolution of organismal performance. We examined the possibility of an evolutionary trade‐off between aquatic and terrestrial locomotion in females of European species of the newt genus Triturus. Biomechanical models suggest a conflict between the requirements for aquatic and terrestrial locomotion. For instance, having an elongate, slender body, a large tail, and reduced limbs should benefit undulatory swimming, but at the cost of reduced running capacity. To test the prediction of an evolutionary trade‐off between swimming and running capacity, we investigated relationships between size‐corrected morphology and maximum locomotor performance in females of ten species of newts. Phylogenetic comparative analyses revealed that an evolutionary trend of body elongation (increasing axilla‐groin distance) is associated with a reduction in head width and forelimb length. Body elongation resulted in reduced maximum running speed, but, surprisingly, also led to a reduction in swimming speed. The evolution of longer tails was associated with an increase in maximal swimming speed. We found no evidence for an evolutionary trade‐off between aquatic and terrestrial locomotor performance, probably because of the unexpected negative effect of body elongation on swimming speed. We conclude that the idea of a design conflict between aquatic and terrestrial locomotion, mediated through antagonistic effects of body elongation, does not apply to our model system.

Effect of Fatigue on Stroking Characteristics in an Arms-Only 100-m Front-Crawl Race

Race analyses during swimming provide information on the time-dependent values of, among other variables, a swimmer's clean swimming speed (v), stroke rate (SR), and stroke length (SL). The effect of fatigue on changes in v, SR, and SL was investigated. Lap-averaged values of v, SR, and SL while swimming 100 m all out with arms only (trial 1) were related to the decline in mechanical power output measured during an all-out 100-m swim on the MAD system (trial 2), with legs immobilized in both trials. Swimming a 100-m front-crawl sprint using arms only led to a significant 24% decrease in lap-averaged mechanical power output (from 200 to 153 W, P < 0.01). This reduction in power-generating capacity led to a 12.4% decrease in v when lap 1 was compared with lap 4 (from 1.69 to 1.48 m x s(-1), P < 0.01). SR declined throughout the race by 10.6% from 0.85 Hz (lap 1) to 0.76 Hz (lap 4, P < 0.05). Analysis revealed that this decrease in SR was linearly related to the decrease in v. The reduction in SR reflected the reduced propulsive force required to overcome the v-dependent, and therefore lower, drag. Decreases in swimming speed throughout a 100-m front-crawl race are the result of decreases in the power-producing capacity of the swimmer (fatigue). This fatigue-induced reduction in swimming speed will lead to a reduction in drag. The SR seems to be accommodated to this reduced power output capacity and concomitant diminished propulsion requirements.

Good eaters, poor swimmers: compromises in larval form

Compromises between swimming and feeding affect larval form and behavior. Two hypotheses, with supporting examples, illustrate these feeding-swimming trade-offs. (1) Extension of ciliated bands into long loops increases maximum clearance rates in feeding but can decrease stability of swimming in shear flows. A hydromechanical model of swimming by ciliated bands on arms indicates that morphologies with high performance in swimming speed and weight-carrying ability in still water differ from morphologies conferring high stability to external disturbances such as shear flows. Instability includes movement across flow lines from upwelling to downwelling water in vertical shear. Thus a hypothesis for the high arm elevation angles of sea urchin larvae, which reduce speed in still water, is that they reduce a downward bias imposed by the vertical shear in turbulence. Observations of sea urchin larvae in vertical shear and comparisons among brittle star larvae are consistent with the performance trade-offs predicted by the model. (2) Structures and behaviors that reduce swimming speed can enhance filtering for feeding. In the opposed-band feeding mechanisms of veligers and many trochophores, cilia push water to swim but movement of cilia relative to the water occurs when cilia overtake and capture particles. Features that may increase clearance rates at the expense of speed and weight capacity include structures that increase drag or body weight and a ciliary band that beats in opposition to the feeding-swimming current. Larval feeding mechanisms inherited from distant ancestors result in different swimming-feeding trade-offs. The different trade-offs further diversify larval form and behavior.

TRITURUS NEWTS DEFY THE RUNNING-SWIMMING DILEMMA

Conflicts between structural requirements for carrying out different ecologically relevant functions may result in a compromise phenotype that maximizes neither function. Identifying and evaluating functional trade-offs may therefore aid in understanding the evolution of organismal performance. We examined the possibility of an evolutionary trade-off between aquatic and terrestrial locomotion in females of European species of the newt genus Triturus. Biomechanical models suggest a conflict between the requirements for aquatic and terrestrial locomotion. For instance, having an elongate, slender body, a large tail, and reduced limbs should benefit undulatory swimming, but at the cost of reduced running capacity. To test the prediction of an evolutionary trade-off between swimming and running capacity, we investigated relationships between size-corrected morphology and maximum locomotor performance in females of ten species of newts. Phylogenetic comparative analyses revealed that an evolutionary trend of body elongation (increasing axilla-groin distance) is associated with a reduction in head width and forelimb length. Body elongation resulted in reduced maximum running speed, but, surprisingly, also led to a reduction in swimming speed. The evolution of longer tails was associated with an increase in maximal swimming speed. We found no evidence for an evolutionary trade-off between aquatic and terrestrial locomotor performance, probably because of the unexpected negative effect of body elongation on swimming speed. We conclude that the idea of a design conflict between aquatic and terrestrial locomotion, mediated through antagonistic effects of body elongation, does not apply to our model system.

The vertical component of a fish's spatial map

Fish orient by using a memorized map of their surroundings and, as they live in aquatic environments, this map must be three dimensional. Studies have shown that fish are able to encode horizontal information; however, we have little information about the cues that are learnt and remembered in the vertical axis. We used the blind Mexican cave fish, Astyanax fasciatus, to test whether fish can orient in the vertical axis and whether they use hydrostatic pressure to do so. After learning the cues associated with a specific height in the aquarium (evident by a reduction in swimming speed), the fish showed significant dishabituation (their swimming speed increased) when moved vertically upwards. When we controlled for the increase in light intensity from the bottom to the top of the tank the effect persisted, indicating that the most likely cue used by the fish to orient vertically was hydrostatic pressure. A post hoc experiment revealed that the change in swimming speed in response to altered light levels probably represents a change in the level of activity rather than an orientational response. This may define a photokinetic mechanism which operates in conjunction with a negatively phototactic response (revealed in previous experiments) by which the fish orient away from higher light levels and swim faster as light levels increase. This would result in the fish spending more time in lower light levels and would prevent the fish exiting the cave.

Potassium homeostasis influences the locomotion and encystment of zoospores of plant pathogenic oomycetes

Zoospores of plant pathogenic oomycetes exhibit distinct swimming speeds and patterns under natural conditions. Zoospore swimming is influenced by ion homeostasis and changes in the ionic composition of media. Therefore, we used video microscopy to investigate swimming patterns of five oomycete species in response to changes in potassium homeostasis. In general, zoospore speed tended to be negatively correlated with zoospore size. Three Phytophthora species ( Phytophthora palmivora, Phytophthora megakarya, and Phytophthora infestans) swam in straight patterns with speeds ranging from 50 to 250 μm/s whereas two Pythium species ( Pythium aphanidermatum and Pythium dissotocum) swam at similar speeds ranging from 180 to 225 μm/s with a pronounced helical trajectory and varying amplitudes. High external concentrations of potassium salts reduced the swimming speed of Ph. palmivora and induced encystment. This was not observed for Py. aphanidermatum. Application of the potassium ionophores gramicidin, nigericin and valinomycin resulted in reduced swimming speeds and changes in the swimming patterns of the Phytophthora species. Therefore, potassium ions play a key role in regulating zoospore behavior.

Laboratory evidence for behavioural impairment of fish escaping trawls: a review

Abstract It is now widely accepted that for some species a proportion of the undersized fish escaping trawl codends die as a direct result of stress, with 10% to 30% mortality commonly cited. It has also been suggested that there may be indirect or behaviourally mediated mortality; fish that encounter and escape the trawl, only to experience stress-induced behavioural deficits and succumb to predators in the hours or days afterwards. The goal of this review was to evaluate the plausibility of this behaviourally mediated, yet unobserved mortality. Three laboratory studies utilizing cod (Gadus morhua), walleye pollock (Theragra chalcogramma), and sablefish (Anoplopoma fimbria) have assayed for behavioural impairment in fish following application of stressors designed to simulate entrainment and escape from trawls. Where impairments in anti-predator capabilities occurred, it was determined that trawl-stressed fish exhibited reduced swimming speed, reduced shoal cohesion, and reduced predator vigilance compared to control fish. Although stressed fish appeared to rapidly recover their ability to avoid being eaten by predators, measurements of more subtle aspects of escapee behaviour suggest that impairments may persist for days after stressor application. Although these studies demonstrate that more investigation is required, when combined with a more extensive literature demonstrating that a variety of stressors can impair fish anti-predator behaviour, it is reasonable to conclude that many fish species escaping trawl codends will likely suffer behavioural deficits that subject them to elevated predation risk. As such, there is probably mortality associated with trawl fisheries that is generally unrecognized, unmeasured, and unaccounted for in current stock assessment models. Further, these studies demonstrate that behavioural competency needs to be considered in the design and implementation of by-catch reduction devises and strategies.

The effect of demand feeding on swimming speed and feeding responses in Atlantic salmon Salmo salar L., gilthead sea bream Sparus aurata L. and European sea bass Dicentrarchus labrax L. in sea cages

Using underwater cameras, data were collected on the feeding behaviour and swimming speeds of Atlantic salmon Salmo salar L., gilthead sea bream Sparus aurata L. and European sea bass Dicentrarchus labrax L. in sea cages. Comparisons were made between the behaviours of fish fed on demand using interactive feedback systems and those of fish fed under the standard feeding practice of each farm (control). In all three species, swimming speeds were similar before feeding, but they were significantly higher in the control regimes during feeding. When fed on demand, sea bass had reduced swimming speed just before and during feeding compared with that observed during the non-feeding periods. Higher proportions of feeding fish were observed in the control regime cages than in fish fed on demand for all three species, indicating a greater feeding intensity during meals in the control regimes. This was further supported by observations of an increase in the density of sea bass in the upper water in the control cages during feeding. The results suggest decreased levels of competition between the on demand-fed fish during feeding, which might be hypothesized to lead to improved growth and production efficiency in aquaculture.

The Effects of Long-Term Treatment with Metrifonate, a Cholinesterase Inhibitor, on Cholinergic Activity, Amyloid Pathology, and Cognitive Function in APP and PS1 Doubly Transgenic Mice

Recent studies in cell cultures have shown that modulating the cholinergic activity can influence the processing and metabolism of amyloid precursor protein (APP). To investigate whether acetylcholinesterase inhibitors (ChEIs) could decrease production of amyloid β-peptide (Aβ) and slow down the accumulation of Aβ also in vivo, we chronically administered metrifonate (100 mg/kg, po), a second-generation ChEI, to 7-month-old doubly transgenic APP+PS1 mice and their nontransgenic littermate controls for 7 months. Behavioral studies, including open field test, T maze, and water maze, were conducted after 6 months treatment with metrifonate, and the mice were sacrificed at the age of 14 months for biochemical and histological analyses. The long-term treatment with metrifonate failed to inhibit the marked overproduction and deposition of Aβ in the APP+PS1 mice; in contrast, it increased both Aβ40 and Aβ42 levels in the hippocampus. However, the Aβ42 to 40 ratio was significantly reduced by the treatment. In addition, the number of amyloid plaques in the hippocampus did not differ between the treatment and the control groups. Tolerance to cholinesterase inhibition might be induced in the mouse brain because the inhibition rate of AChE was attenuated from about 80 to 50% during the experiment in both APP+PS1 and nontransgenic mice. The metrifonate treatment did not affect cognitive testing parameters but reduced swimming speed and locomotor activity in both genotypes. Our results do not support the idea that ChEIs would slow down the progression of amyloid pathology in Alzheimer's disease.

Behavioural response of Daphnia to olfactory cues from food, competitors and predators

The behavioural response of the freshwater zooplankter Daphnia to chemicals from its food, the green alga Scenedesmus, to algal cells, to the green colour from chlorophyll, to chemicals released from congeners and conspecifics, and to chemicals released from invertebrate (Chaoborus) and vertebrate predators (Leuciscus) was investigated in a Y-tube olfactometer. No preference was observed either for medium that had contained algae, or for medium with algal cells or with the green colour of algae, offered as alternatives to clean medium. In contrast, swimming speed was significantly reduced at high algal concentrations and in the presence of green colour. Moreover, starved animals had lost their rheotaxis. Neither Daphnia magna nor Daphnia pulex had a preference for either clean medium or medium that had contained conspecifics, but D. magna significantly chose clean medium when medium which had been inhabited by D. pulex was the alternative. No avoidance of medium from Chaoborus cultures was found, but D. magna significantly avoided medium that had been inhabited by ides (Leuciscus idus L.). The responses observed could result in aggregation of animals by reduced swimming speed at high algal densities and by avoidance of areas with predators. When these cues have become less important due to food depletion and decreased predation pressure, the interspecific-competitor-related cues might result in desegregation.

The functional response of a predatory plant preying on swarming zooplankton

In a laboratory study, we determined the functional response of the carnivorous aquatic plant Utricularia vulgaris feeding on Polyphemus pediculus, a cladoceran zooplankton that forms swarms. The number of prey eaten increased linearly with prey density up to a density of 35 prey per 125 ml and decreased slightly above this density. Independent estimates of handling time showed that the number eaten was not limited by handling. Thus, we hypothesized that the functional response levelled off because attack rate decreased with increasing density. Direct observations of the predation act at high and low prey densities showed that prey per capita mortality rate was markedly lower at high densities. An analysis of the components of the predation cycle showed that encounter rate and attack probability but not capture success decreased with increasing prey density. We, then, studied the degree of aggregation and the movement behaviour of Polyphemus. The tendency to form swarms increased with density and this was associated with reduced swimming speed and swimming along a more tortuous path. Presence of Utricularia leaves did not influence the spatial distribution and swimming behaviour of Polyphemus. We concluded that the unusual shape of the functional response was due to density dependent prey mortality rates that resulted from a density dependent tendency to form swarms. We, therefore, suggested a modification of Holling's type II functional response model that included density dependent attack rate and this model fitted data significantly better than the original model.

Systemic Administration of Atipamezole, a Selective Antagonist of Alpha-2 Adrenoceptors, Facilitates Behavioural Activity but does not Influence Short-term or Long-term Memory in Trimethyltin-intoxicated and Control Rats

NIITTYKOSKI M., R. LAPPALAINEN, J. JOLKKONEN, A. HAAPALINNA, P. RIEKKINEN Sr and J. SIRVIÖ. Systemic administration of atipamezole, a selective antagonist of alpha-2 adrenoceptors, facilitates behavioural activity but does not influence short-term or long-term memory in trimethyltin-intoxicated and control rats. NEUROSCI BIOBEHAV REV 22(6) 735–750, 1998.—The present study used trimethyltin (TMT)-intoxicated rats as a model for the behavioural syndrome seen after neuronal damage to the limbic system. Behavioural assessments indicated increased locomotor activity and reduced number of groomings in an open-arena task in TMT-intoxicated (6.6 mg/kg as a free base) rats, as has been found previously. A novel finding was the severe deficit in swimming to a visible platform in the water maze task, with reduced swimming speed at the beginning of the training period. During the reacquisition phase of a radial arm maze task, TMT-intoxicated rats made more short-term and long-term memory errors, and their behavioural activity was increased in comparison with controls. The administration of atipamezole (300 μg/kg), a selective antagonist of α 2-adrenoceptors, enhanced locomotor activity compared to saline-treated rats, but these effects did not differ between the TMT group and their controls. Atipamezole did not enhance short-term or long-term memory in either TMT or control groups. Taken together, the present data indicate that TMT intoxication is a model for global dementia rather than for a specific loss of relational memory. Previous studies on the neurochemical effects of TMT and the alleviation or prevention of neurotoxicity of TMT are reviewed.

Hydrodynamic effect of a satellite transmitter on a juvenile green turtle (Chelonia mydas)

Wind tunnel tests were performed to measure the effect of a satellite transmitter on a juvenile green turtle (Chelonia mydas). A full-scale turtle model was constructed from an 11.5 kg specimen with a 48 cm carapace length, and a transmitter model was constructed from a Telonics ST-6. The turtle model was tested in a wind tunnel with and without the transmitter, which was mounted on the forward, topmost part of the carapace. Drag, lift and pitch moment were measured for several speeds and flow angles, and the data were scaled for application to the marine environment. At small flow angles representative of straight-line swimming, the transmitter increased drag by 27-30 %, reduced lift by less than 10 % and increased the pitch moment by 11-42 %. On the basis of the drag data at zero angle of attack, it is estimated that the backpack will reduce swimming speed by 11 %, assuming that the turtle produces the same thrust with the unit attached. The drag data are also used to estimate the effect of a transmitter on the swimming energetics of an adult green turtle. Design guidelines are included to minimize the adverse forces and moments caused by the transmitter.

Parasitation with Pseudoterranova decipiens (Nematoda) influences the survival rate of the European smelt Osmerus eperlanus retained by a screen wall of a nuclear power plant.

A total of 354 adult European smelts Osmerus eperlanus (L.) were tested for their ability to survive the screen system of the cooling water inflow of a power plant. With increasing number of musculature parasitic third-stage larvae of Pseudoterranova decipiens, the survival rate of O. eperlanus decreased while the total number of externally visible injuries as well as the number of seriously injured specimens increased. The results indicate that even a single specimen of P. decipiens influences resistance and stamina and affects overall mortality of 7 to 20 cm long smelts. The initial effect of the parasites is to reduce swimming speed of infested fish, which leads to more frequent contact of these fish with the fine meshed screen of the cooling water inlet before they are removed by the automatic cleaning system. If the separated fishes are returned to the main stream, it becomes apparent that the cooling water inflow selectively reduces the number of living parasitised smelt in the area. Thus, the number of parasitic third-stage P. decipiens larvae in the local smelt population which are able to complete their life-cycle is also reduced. P. decipiens makes infested smelt more susceptible to negative anthropogenic influences such as cooling water intake or trawl fisheries.

Systemic administration of atipamezole, an ??2-antagonist, can reduce scopolamine-induced hyperactivity in rats

The present study investigated whether an alpha 2-adrenoceptor antagonist (atipamezole) can influence hyperactivity induced by the systemic administration of scopolamine. In the water maze (WM) task, scopolamine (scop) 0.25 mg/kg treatment significantly increased swimming speed during the acquisition phase of this task. Atipamezole (ati) 30 micrograms/kg slightly reduced swimming speed both in saline- and in scop-treated rats. Ati 300 micrograms/kg slightly reduced swimming speed in saline-treated rats, and it prevented the scop-induced increase in swimming speed, because ati300-scop treated rats swam more slowly than the saline-saline group. In addition, ati 300 micrograms/kg reduced the total distance swum in scop-treated rats during a free swim trial (the platform was removed from the pool) performed 1 day after the acquisition phase of the WM test, even though it did not affect this parameter when administered alone. In the open arena task, which assessed the ambulation of rats when the pool was covered with a solid floor, scopolamine dose-dependently increased locomotor activity. The rats ambulated more when treated with scop 0.50 mg/kg compared to vehicle treatment, whereas the effect of scop 0.25 mg/kg treatment did not reach significance. In a test investigating the effects of ati 300 micrograms/kg and scop 0.50 mg/kg given singly or combined, the rats ambulated more during both ati 300 micrograms/kg and scop 0.50 mg/kg treatments given alone, but when combined, the rats ambulated less than during scop-saline treatment even though this was more than during control (saline-saline) treatment. These results indicate that the systemic administration of an alpha 2-antagonist can reduce hyperactivity induced by scopolamine.

Physiological effects of the detergent linear alkylbenzene sulphonate on blue mussel larvae ( Mytilus edulis ) in laboratory and mesocosm experiments

A series of laboratory (short-term exposure in small beakers) studies and a 19 d mesocosm (6 m3 polyethylene bags filled with fjord water) study were conducted on blue mussel, Mytilus edulis, larvae and plantigrades exposed to a concentration gradient of the detergent linear alkylbenzene sulphonate (LAS, 0 to 39 mg l−1). LAS is increasingly found in nearshore environments receiving wastewater from urban treatment plants. The aims were to observe physiological effects on swimming, grazing and growth in the laboratory and effects on settling and population development at in situ conditions (in field mesocosms) in order to evaluate the damages on ciliated meroplankton caused by LAS. In the laboratory the larvae showed a 50% mortality at 3.8 mg LAS l−1 after 96 h exposure whether or not food was provided. Additionally the swimming behaviour was affected at 0.8 mg LAS l−1 (i.e. a more compact swimming track, a smaller diameter of the swimming tracks, and reduced swimming speed). The larval particle grazing was reduced 50% at 1.4 mg LAS l−1. The specific growth rate of the larvae was reduced to half at 0.82 mg LAS l−1 over 9 d. During the mesocosm experiment, the larval population showed a dramatic decrease in abundance within 2 d at concentrations as low as 0.08 mg LAS l−1, both due to a significantly increased mortality, but also due to settling. The settling success was reduced at the same LAS concentration as that at which mortality was observed to increase significantly. In addition to reduced settling rate, the larvae showed delayed metamorphosis and reduced shell growth as a response to LAS. Our hypothesis that the larval ciliary apparatus, crucial for normal swimming, orientation, and settling behaviours and for particle uptake, was damaged due to LAS exposure is supported by our results. This is confirmed by the physiological data (grazing, growth) and in the direct video-based observations of larval performance (swimming) and provides a reasonable explanation for what was observed in the bags (abundance, settling, mortality). These physiological effects on blue mussel larvae/plantigrades occurred at LAS concentrations reported to occur in estuarine waters.

Membrane toxicity of opioids measured by protozoan motility

The acute toxicity of some opioid drugs cannot solely be explained by a specific interaction with the opioid receptor. The anaesthetic-like membrane effect of 10 opioid agents and the antagonist naloxone was determined and correlated with their hydrophobicity. The inhibitory effect of drugs on protozoan motility was used as a measure of their membrane toxicity, measured by the reduction in swimming speed of Tetrahymena pyriformis using an image analysis system. Hydrophobicity was determined as the n-octanol/water partition coefficient, at pH 7.4, 37 °C. Opioid agents dose-dependently reduced the swimming speed of Tetrahymena pyriformis with a wide range of IC50 values. Some weak opioid agents were shown to have high protozoan immobilising potency comparable to quinidine, an agent with known membrane stabilising activity. Norpropoxyphene, the metabolite of dextropropoxyphene, with little affinity for the opioid receptor, also had a high potency. The inhibition of protozoan motility by these opioid agents was not antagonised by the opioid receptor antagonist naloxone; moreover an additive inhibitory action was demonstrated when opioid agents were combined with naloxone. The effect of opioid agents on protozoan motility was closely correlated with their partition coefficient but not with their known affinity for opioid receptors. These results suggest that opioid agents possess differing degrees of membrane depressant action independent from their interaction with the opioid receptor, and have a potential for causing depressant effects on excitable tissues.

The functional morphology of caudal lamellae in coenagrionid (Odonata:Zygoptera) damselfly larvae

During their later instars, coenagrionid damselfly larvae reduce their relative investment in lamellae. Caudal lamellae play a role in swimming by allowing a larva to take advantage of its body size when at least one lamella is present, and this benefit increases with larval size. The removal of the first and/or second lamella generally results in a fixed and measurable decrease in swimming speed. When all lamellae are removed, larvae suffer a dramatic reduction in swimming speed. Ischnura posita(Hagen) and Enallagma civile(Hagen) always swim faster than equal sized Telebasis salva(Hagen) and Argia translata(Hagen) when at least one lamella is present. The swimming speed of the three lentic forms I. posita, E. civile,and T. salvais more affected by lamella loss than the lotic form A. translata.These last three relationships do not change ontogenetically. Caudal lamellae surface area does not increase as fast ontogenetically as would be expected for a gas exchange organ. The breaking joints, which hold the lamellae to the body, do not grow isometrically with body size, but do grow isometrically with lamellar mass. This suggests that the lamellae may be retained in proportion to investment (mass).

Reduced swimming performance and gill structural changes in juvenile salmonids exposed to 2-(thiocyanomethylthio)benzothiazole

The toxicity of the wood preservative agent 2-(thiocyanomethylthio)benzothiazole (TCMTB) was evaluated in three species of juvenile salmonids ( Oncorhynchus kisutch, O. tshawytscha and O. mykiss). Gill tissues were examined for quantitative structural changes after a 96-h LC 50 acute test. TCMTB was found to have both a concentration- and time-dependent effect on gill histology with damage apparent at concentrations as low as 6 μg·1 −1. The residual effect on swimming performance of a 12 to 48 h sublethal TCMTB exposure was also examined. Concentration dependent reductions in swimming speed were observed with thresholds of 5 to 10 μg·1 −1. The inhibitory effect on swimming performance was also found to be intensified by an increase in exposure duration. The relationship between the inhibitory effect on swimming speed and the observed gill damage was examined. These reductions in swimming speed may represent a limitation in gill oxygen transfer capacity resulting from TCMTB-induced alteration of gill structure.