Reduced Muscle Activity Research Articles

Kinematic and electromyographic studies on unaided, unilateral and bilateral crutch walking in adolescents with spastic diplegia

Back pain and ambulation deterioration among ambulatory individuals with cerebral palsy (CP) are common as they grow older and walking aids are often prescribed to improve stability and promote maximal weight-bear on lower limbs during gait. To investigate the effects of walking aids on back muscle activity and whole body kinematics among adolescents with spastic diplegia. A repeated-measures design was adopted with participants tested under different walking conditions. Ten participants were recruited and Lofstrand forearm crutches were selected. Both the activity of lumbar erector spinae and the kinematics of head, trunk, pelvis and lower limbs during walking were monitored using telemetric electromyography and motion analysis system respectively. Comparisons between walking unaided and walking with unilateral and bilateral crutch(es) were made. Significant decreases in speed, cadence, erector spinae activity and lower trunk extension were observed during crutch walking together with significantly increased stride time and anterior pelvic tilt. These findings suggested that Lofstrand crutch(es) reduced muscular demands and lumbar lordosis with increased lower back mobility. The results shed light on the prescription of walking aid in the management and prevention of chronic back pain for ambulatory individuals with CP from a life span perspective.

Voluntary resistance wheel exercise during post‐natal growth in rats enhances skeletal muscle satellite cell and myonuclear content at adulthood

To determine whether voluntary free wheel (FW) or resistance wheel (RW) exercise or reduced muscle activity would influence maturational increases in muscle mass and the number of satellite cells (SCs) and myonuclei (MN) accrued by adulthood. Hind limb muscles of male rats housed with, or without, FWs from 4 to 5, 7 or 10 weeks of age, and rats housed with RWs from 4 to 10 week of age, were evaluated. To assess the effect of reduced muscle activity, gastrocnemius muscles of 4-week-old rats were injected with botulinum toxin (Btx) and collected at 7 weeks of age. Muscle fibre size and the frequency of Pax7-positive SCs and MN were determined in 7- and 10-week-old muscles via immunohistochemical methods. Free wheel exercise enhanced muscle growth and the frequency of SCs in the medial gastrocnemius (MG) (threefold) and vastus lateralis (VL) (twofold) of rats at 10 week of age. Resistance wheel exercise increased the number of SCs and MN (22-30%), with more muscle fibre nuclei being associated with larger fibre size, in the soleus, MG and VL muscles. Btx impaired the normal increases in muscle fibre size and the accrual of MN but not SCs. A greater volume of exercise during maturational growth was important for enhancing SC numbers, whereas their conversion to MN required higher-intensity exercise. The enhanced muscle fibre nuclear populations may influence the capacity of the muscle to adapt to exercise, injury or disuse in later adulthood.

Paper # 215: Muscle Architectural and Functional Differences Between Traditional vs. Early Active Rehabilitation Programs After Achilles Tendon Repair

The results of this study showed that an accelerated rehabilitation protocol improved muscle architecture and generated a smaller reduction in isokinetic torque and a larger reduction in muscle activation at high angular velocities compared to a traditional treatment, showing that this rehabilitation program was effective and safe.

Thermodynamics investigation of Ca2+ effect on Troponin-C (TnC): A QM/MM study

Troponin (Tn) is an essential protein in calcium ion settings of skeletal and heart muscle contractions. It has three subcategories (TnC, TnI, TnT) which are connected to filament actin by tropomyosin. TnC controls the calcium in muscle contraction and is the Ca2+-binding subunit of the troponin complex. ATP facilitates separation of myosin from actin filament, eventually making thick filament to slip off thin filament. This results in short sarcomere. In this study, it is investigated that thermodynamics properties increase concentrations of Ca2+ and temperature present in lactic acid. Quantum mechanical and molecular dynamics methods were used to analyze the structure and stability of TnC-Ca2+configuration. TnC-Ca2+ mutated active site in lactic acid and water solvent. Calcium ions approaching TnC caused twisting movement of TnC, so TnI-TnT eventually released the head of Myozin to actin. Increased lactic acid can increase calcium ion connection around TnC and decrease Ca to SR; increased temperature can increase metabolism and fatigue of skeletal muscles, emptying glycolysis and reducing muscle activity. Key words: Troponin C (TnC), calcium ion (Ca2+), lactic acid, fatigue of skeletal muscles, QM/MM.

Muscle activations under varying lifting speeds and intensities during bench press

During a set of resistance exercise performed until exhaustion, the relationship between intensity and the number of repetitions can be affected by lifting speed, with faster speeds producing higher numbers. The hypothesized mechanisms include enhanced utilization of the stretch-shortening cycle. This study investigated muscle activations under varying speeds and intensities during bench press using surface electromyography (EMG) to suggest further mechanisms for the above finding. Thirteen weight-trained men (21.7 ± 3.6-year-old) performed bench press until fatigue under five intensities (40-80% 1RM), and four speeds (slow 5.6-s/repetition, medium 2.8-s/repetition, fast 1.9-s/repetition, and ballistic maximum speed). Surface EMG was recorded from the pectoralis, deltoid, and triceps for root-mean-square amplitude and median frequency. EMG amplitudes were greater for faster and heavier conditions before fatigue. Faster conditions, however, produced a significant fall in amplitude during the final concentric phase compared to slower movements. After fatigue, EMG amplitude increased, with the speed effect being maintained. The intensity effect on amplitude either disappeared or remained similar, depending on the muscles. Median frequencies before fatigue were similar among speeds and intensities. The fall in frequency after fatigue was similar across speeds, but greater for lighter intensities. It was concluded that reduced muscle activation during the final concentric phase in faster conditions allowed a better muscle pump, explaining the increased repetition numbers. Fatigue levels are likely to have been similar across speeds, but greater for lower intensities. An incomplete rise in EMG amplitude after fatigue for lower intensities could imply an increased contribution of central fatigue or neuromuscular transmission failure.

스위스 볼을 이용한 등척성, 등장성 운동이 요부 안정화 근육의 근활성도 및 통증에 미치는 영향

Purpose : The purpose of this study is to examine the influence of a core stability exercise program using Swiss Ball on muscle activity, muscle thickness, maximum muscular strength, and pain in the trunk region during a 6-weeks program involving participants who have lower back pain. Methods : A total of 21 males between the ages of 20 and 33 years old were divided into 3 Swiss Ball exercise groups. Group 1 performed isometric exercises, group 2 performed isotonic exercises, and group 3 performed mixed exercises. Measurements were taken prior to starting the exercise program and after completing the program at the 6-week period using ME6000 to measure muscle activity and VAS to measure pain reduction. Comparisons were made using a paired t-test and ANOVA on SPSS 10.0. Results : There was a statistically significant effect in muscle activity for group 1 and group 3. Secondly, there was a statistically significant effect in pain reduction for group 1, group 2, and group 3. Conclusion : We found that isometric exercises performed by group 1 were effective in improving muscle activity and pain reduction.

Neuromuscular adjustments that constrain submaximal EMG amplitude at task failure of sustained isometric contractions

The amplitude of the surface EMG does not reach the level achieved during a maximal voluntary contraction force at the end of a sustained, submaximal contraction, despite near-maximal levels of voluntary effort. The depression of EMG amplitude may be explained by several neural and muscular adjustments during fatiguing contractions, including decreased net neural drive to the muscle, changes in the shape of the motor unit action potentials, and EMG amplitude cancellation. The changes in these parameters for the entire motor unit pool, however, cannot be measured experimentally. The present study used a computational model to simulate the adjustments during sustained isometric contractions and thereby determine the relative importance of these factors in explaining the submaximal levels of EMG amplitude at task failure. The simulation results indicated that the amount of amplitude cancellation in the simulated EMG (∼ 40%) exhibited a negligible change during the fatiguing contractions. Instead, the main determinant of the submaximal EMG amplitude at task failure was a decrease in muscle activation (number of muscle fiber action potentials), due to a reduction in the net synaptic input to motor neurons, with a lesser contribution from changes in the shape of the motor unit action potentials. Despite the association between the submaximal EMG amplitude and reduced muscle activation, the deficit in EMG amplitude at task failure was not consistently associated with the decrease in neural drive (number of motor unit action potentials) to the muscle. This indicates that the EMG amplitude cannot be used as an index of neural drive.

Activation timing of postural muscles during bilateral arm flexion in self-timing, oddball and simple-reaction tasks

Background We investigated the effect of time constraint on activation timing of postural muscles during bilateral arm flexion in self-timing, oddball and simple-reaction tasks. Methods Thirteen healthy adults flexed their arms from a suspended position with maximum speed and stopped at the shoulder level. For erector spinae-longissimus (ES), biceps femoris (BF), and gastrocnemius (GcM), onset timing of burst activation with respect to the anterior deltoid (AD), and the displacement of the center of pressure in the anteroposterior direction (CoPy) were analyzed. Results AD reaction time was significantly shorter in the simple-reaction task than oddball task, suggesting that time constraint would be lower in the task order noted above and affected the state of postural preparation. The following properties were found in the onset timing of postural muscle: (1) the onset timing of BF and GcM were earlier in the task order noted above, and (2) the earliest activated distal muscle in the self-timing task was activated later in the simple-reaction task. CoPy displacement was smaller in the same task order as the onset timing. Conclusions It appears that with sufficient postural preparation, the focus of postural control is on the reduction of postural disturbance and earlier lower leg muscle activation.

Characterization of sarcoplasmic calcium binding protein (SCP) variants from freshwater crayfish Procambarus clarkii

Sarcoplasmic calcium binding protein (SCP) is an invertebrate EF-hand calcium buffering protein that has been proposed to fulfill a similar function in muscle relaxation as vertebrate parvalbumin. We have identified three SCP variants in the freshwater crayfish Procambarus clarkii. The variants (pcSCP1a, pcSCP1b, and pcSCP1c) differ across a 37 amino acid region that lies mainly between the second and third EF-hand calcium binding domains. We evaluated tissue distribution and response of the variants to cold exposure, a stress known to affect expression of parvalbumin. Expression patterns of the variants were not different and therefore do not provide a functional rationale for the polymorphism of pcSCP1. Compared to hepatopancreas, expression of pcSCP1 variants was 100,000-fold greater in axial abdominal muscle and 10-fold greater in cardiac muscle. Expression was 10-100 greater in fast-twitch deep flexor and extensor muscles compared to slow-twitch superficial flexor and extensors. In axial muscle, no significant changes of pcSCP1, calmodulin (CaM), or sarcoplasmic/endoplasmic reticulum Ca-ATPase (SERCA) expression were measured after one week of 4°C exposure. In contrast, large decreases of pcSCP1 were measured in cardiac muscle, with no changes in CaM or SERCA. Knockdown of pcSCP1 by dsRNA led to reduced muscle activity and decreased expression of SERCA. In summary, the pattern of pcSCP1 tissue expression is similar to parvalbumin, supporting a role in muscle contraction. However, the response of pcSCP1 to cold exposure differs from parvalbumin, suggesting possible functional divergence between the two proteins.

Adaptation to continuous perturbation of balance: Progressive reduction of postural muscle activity with invariant or increasing oscillations of the center of mass depending on perturbation frequency and vision conditions

We investigated the adaptation of balancing behavior during a continuous, predictable perturbation of stance consisting of 3-min backward and forward horizontal sinusoidal oscillations of the support base. Two visual conditions (eyes-open, EO; eyes-closed, EC) and two oscillation frequencies (LF, 0.2 Hz; HF, 0.6 Hz) were used. Center of Mass (CoM) and Center of Pressure (CoP) oscillations and EMG of Soleus (Sol) and Tibialis Anterior (TA) were recorded. The time course of each variable was estimated through an exponential model. An adaptation index allowed comparison of the degree of adaptation of different variables. Muscle activity pattern was initially prominent under the more challenging conditions (HF, EC and EO; LF, EC) and diminished progressively to reach a steady state. At HF, the behavior of CoM and CoP was almost invariant. The time-constant of EMG adaptation was shorter for TA than for Sol. With EC, the adaptation index showed a larger decay in the TA than Sol activity at the end of the balancing trial, pointing to a different role of the two muscles in the adaptation process. At LF, CoM and CoP oscillations increased during the balancing trial to match the platform translations. This occurred regardless of the different EMG patterns under EO and EC. Contrary to CoM and CoP, the adaptation of the muscle activities had a similar time-course at both HF and LF, in spite of the two frequencies implying a different number of oscillation cycles. During adaptation, under critical balancing conditions (HF), postural muscle activity is tuned to that sufficient for keeping CoM within narrow limits. On the contrary, at LF, when vision permits, a similar decreasing pattern of muscle activity parallels a progressive increase in CoM oscillation amplitude, and the adaptive balancing behavior shifts from the initially reactive behavior to one of passive riding the platform. Adaptive balance control would rely on on-line computation of risk of falling and sensory inflow, while minimizing balance challenge and muscle effort. The results from this study contribute to the understanding of plasticity of the balance control mechanisms under posture-challenging conditions.

The Effect of a Lightweight Massage System in a Car Seat on Comfort and Electromyogram

Objective The objective of this study was to determine the effect of a lightweight low-intensity massage system (LWMAS) in a car seat on the electromyogram (EMG) of the neck and shoulder muscles and on the comfort experience during driving. Methods Two experiments were performed during driving with and without the active LWMAS in the seat. Subjective measurements were taken, in which the comfort experience was recorded for 20 participants driving a prescribed path around Munich for 120 minutes. Then objective (surface EMG above the rhomboideus and trapezius muscles) measurements and subjective measurements of the comfort experience were recorded over 7 laps on a test track for 24 participants. Results The comfort was higher, and the EMG was significantly lower in the trapezius area while driving with the LWMAS. Conclusion Despite the fact that the LWMAS system is lightweight, has low intensity, and might have had a smaller effect, similar effects to previous studies with heavier systems were found, indicating that this massage system increases comfort and reduces muscle activity during driving as well.

Adaptability of mastication in people with implant-supported bridges

We aimed to determine whether people with implant-supported bridges in both jaws, thus lacking periodontal receptors, adjust jaw muscle activity to food hardness during mastication. Thirteen participants with implant-supported bridges in both jaws and 13 with natural dentition chewed and swallowed soft and hard gelatine-based model foods, while electromyographic (EMG) activity of the masseter and temporal muscles was recorded bilaterally together with the position of the mandible. Data were compared by using a mixed-design anova model and a P-value<0.05 was considered statistically significant. The number of chewing cycles and the duration of the masticatory sequence increased with food hardness in both groups, whereas vertical and lateral amplitude of the jaw movements, and the jaw-opening velocity, increased significantly with food hardness only for the dentate group. Although both groups adapted the EMG activity to the hardness of the food, the implant participants showed a significantly weaker increase in EMG activity with increased food hardness early during the masticatory sequence than the dentate participants did. In addition, the implant group showed significantly less reduction of muscle activity during the progression of the masticatory sequence than the dentate group. People with implant-supported bridges show an impaired adaptation of the muscle activity to food hardness during mastication. We suggest that a lack of sensory signals from periodontal mechanoreceptors accounts for the impairment.

Determination of muscle activity during running at reduced body weight

The aim of this study was to investigate how lower extremity muscles are influenced by body weight support during running at different speeds. Nine participants (age 24 ± 2 years, height 1.75 ± 0.12 m, mass 73.5 ± 15.7 kg) ran at 100%, 115%, and 125% of preferred speed at 100%, 90%, 80%, 70%, and 60% of body weight on a treadmill that provided body weight support. Preferred speed was self-selected by each participant and represented a speed that he or she could sustain if going for a 30 min run. Electromyography (EMG) data were recorded (1000 Hz, 1 min) from the bicep femoris, rectus femoris, tibialis anterior, and gastrocnemius for each condition together with knee angle (electrogoniometer). Average and root mean square EMG were calculated across 30 s. Muscle patterns were determined by smoothing (low-pass filter, 4 Hz) and extracting patterns for 49 cycles defined by consecutive maximum knee flexion angles. Repeated-measures analyses of variance were used to compare average and root mean square across body weight and speeds. Correlations were computed between the 100% speed/100% body weight condition and all other conditions per muscle. There was no interaction between body weight and speed (P > 0.05). Average and root mean square decreased as body weight decreased for all muscles (P < 0.05) and increased across speeds for all muscles (P < 0.05). Correlations for all muscles between conditions were high (range: 0.921–0.999). Although a percent reduction in body weight did not lead to the same reduction in muscle activity, it was clear that reducing body weight leads to a reduction in muscle activity with no changes in muscle activity patterns.

Quantitative Bewegungsanalyse der Rumpfmotorik von Parkinsonpatienten mittels dreidimensionaler Kinematik und Oberflächenelektromyografie

Hintergrund Im Krankheitsverlauf der Parkinsonerkrankung (PD) werden axiale Defizite als Ausdruck einer reduzierten Rumpfmotorik prominent. Obwohl die Rumpfmotorikstörung progressiv verläuft und die tägliche Aktivität der Patienten beeinträchtigt wird, ist die quantitative Beurteilung noch nicht standardisiert und üblich. Methode Wir verglichen die Rumpfmotorik im 3-dimensionalen Raum mit synchroner Oberflächenelektromyografie (oEMG) von 19 PD-Patienten vom akinetisch-rigiden Subtyp (Hoehn & Yahr: 2–3, UPDRS motor score: 25,5 ± 10,7 in „on” Phase) mit einer altersgemachten Kontrollgruppe. Ergebnisse Die Rumpfbeweglichkeit (Range of Motion) war in frontaler, sagittaler und transversaler Ebene in der PD-Gruppe signifikant reduziert (Mittelwerte für alle Bewegungsrichtungen p < 0,001) mit jeweils signifikant erhöhter Bewegungsvariabilität (p < 0,001) bei reduzierter muskulärer Aktivität (oEMG). Die kinematischen Daten korrelierten nicht mit der täglichen L-Dopa-Dosis in mg. Schlussfolgerung Die 3-dimensionale Rumpfkinematik und die analoge Oberflächenelektromyografie ermöglichen den Klinikern und Therapeuten eine quantitative und objektive Beurteilung der Rumpfmotorik bei PD-Patienten. Der fehlende Einfluss von L-Dopa auf die Rumpfkinematik zeigt die unabhängige Kontrolle des axialen Tonus gegenüber des Extremitätentonus.

The masticatory system under varying functional load. Part 1: structural adaptation of rabbit jaw muscles to reduced masticatory load

Skeletal muscle fibres can change their myosin heavy-chain (MyHC) isoform and cross-sectional area, which determine their contraction velocity and maximum force generation, respectively, to adapt to varying functional loads. In general, reduced muscle activity induces transition towards faster fibres and a decrease in fibre cross-sectional area. In order to investigate the effect of a reduction in masticatory load on three functionally different jaw muscles, the MyHC composition and the corresponding cross-sectional area of fibres were determined in the superficial masseter, superficial temporalis, and digastric muscles of male juvenile New Zealand White rabbits that had been raised on a soft diet (n=8) from 8 to 20 weeks of age and in those of normal diet controls (n=8). Differences between groups were tested for statistical significance using a Mann-Whitney rank sum test. The proportion and cross-sectional area of fibres co-expressing MyHC-I and MyHC-cardiac alpha were significantly smaller in the masseter muscles of the animals that had been fed soft food than in those of the controls. In contrast, the proportions and cross-sectional areas of the various fibre types in the temporalis and digastric muscles did not differ significantly between the groups. The results suggest that reducing the masticatory load during development affects the contraction velocity and maximum force generation of the jaw-closing muscles that are primarily responsible for force generation during chewing. These muscles adapt structurally to the reduced functional load with changes in the MyHC composition and cross-sectional area mainly within their slow fibre compartment.

Biofeedback effectiveness to reduce upper limb muscle activity during computer work is muscle specific and time pressure dependent

Continuous electromyographic (EMG) activity level is considered a risk factor in developing muscle disorders. EMG biofeedback is known to be useful in reducing EMG activity in working muscles during computer work. The purpose was to test the following hypotheses: (1) unilateral biofeedback from trapezius (TRA) can reduce bilateral TRA activity but not extensor digitorum communis (EDC) activity; (2) biofeedback from EDC can reduce activity in EDC but not in TRA; (3) biofeedback is more effective in no time constraint than in the time constraint working condition. Eleven healthy women performed computer work during two different working conditions ( time constraint/ no time constraint) while receiving biofeedback. Biofeedback was given from right TRA or EDC through two modes ( visual/ auditory) by the use of EMG or mechanomyography as biofeedback source. During control sessions (no biofeedback), EMG activity was (mean ± SD): 2.4 ± 1.1, 2.5 ± 2.1, and 9.1 ± 3.1%max-EMGrms for right and left TRA and EDC, respectively. During biofeedback from TRA, activity was reduced in right TRA (1.7 ± 1.6%max-EMGrms) and left TRA (1.2 ± 2.0%max-EMGrms) compared to control. During biofeedback from EDC, activity in EDC was reduced (8.3 ± 3.3%max-EMGrms) compared with control. During time constraint, activity was reduced in right TRA (1.9 ± 1.3%max-EMGrms), left TRA (1.5 ± 1.5%max-EMGrms), and EDC (8.4 ± 3.2%max-EMGrms), during biofeedback compared to control. Conclusion: biofeedback reduced muscle activity in TRA by ∼30–50% and in EDC by ∼10% when given from the homologous or bilateral muscle but not from the remote muscle, and was significant in the time constraint condition; while feedback source and presentation mode showed only minor differences in the effect on reducing homologous muscle activity. This implies that biofeedback should be given from the most affected muscle in the occupational setting for targeting relief and prevention of muscle pain most effectively.

Dietary fish oil alleviates soleus atrophy during immobilization in association with Akt signaling to p70s6k and E3 ubiquitin ligases in rats

Reduced muscle activity leads to impaired insulin signaling, which leads to loss of contractile proteins and muscle mass via the Akt pathway. Dietary fish oil rich in long chain n-3 polyunsaturated fatty acids has been shown to prevent insulin signaling resistance in skeletal muscle. This study was conducted to elucidate the protective effect of dietary fish oil on disuse-induced perturbations in insulin signaling and soleus muscle atrophy. To accomplish this, rats were fed a corn-oil- (control) or fish-oil-based diet for 2 weeks, and then subjected to hindlimb immobilization while still receiving the same diets. After 10 days of immobilization, the soleus muscle mass and myosin heavy chain level had markedly decreased; however, these losses were significantly suppressed in rats fed dietary fish oil, compared with the control group. Dietary fish oil nearly completely attenuated the disturbances in activation of the Akt and p70 S6 kinase proteins, as well as the gene expression of muscle-specific E3 ubiquitin ligases (muscle atrophy F-box and muscle RING finger 1). However, insulin receptor substrate 1 associated with the p85 subunit of phosphoinositide 3-kinase was not altered during immobilization. Dietary fish oil also inhibited alterations in the gene expression of cyclooxygenase-2 and inducible nitric oxide synthase, with no additional observation of oxidative stress. Collectively, these findings indicate that dietary fish oil prior to and during immobilization may alleviate the immobilization-induced soleus muscle atrophy, at least in part, via the Akt pathway through E3 ubiquitin ligases and p70s6k.

Effects of Fatigue on Kinetic and Kinematic Variables During a 60-Second Repeated Jumps Test

The aim of this study was to investigate the effects of a maximal repeated-jumps task on force production, muscle activation and kinematics, and to determine if changes in performance were dependent on gender. Eleven male and nine female athletes performed continuous countermovement jumps for 60 s on a force platform while muscle activation was assessed using surface electromyography. Performances were videotaped and digitized (60 Hz). Data were averaged across three jumps in 10-s intervals from the initial jump to the final 10 s of the test. No interaction between time and gender was evident for any variable; therefore, all results represent data collapsed across gender. Preactivation magnitude decreased across time periods for anterior tibialis (AT, P < .001), gastrocnemius (GAS, P < .001) and biceps femoris (BF, P = .03), but not for vastus lateralis (VL, P = .16). Muscle activation during ground contact did not change across time for BF; however, VL, G, and AT showed significant reductions (all P < .001). Peak force was reduced at 40 s compared with the initial jumps, and continued to be reduced at 50 and 60 s (all P < .05). The time from peak force to takeoff was greater at 50 and 60 s compared with the initial jumps (P < .05). Both knee flexion and ankle dorsiflexion were reduced across time (both P < .001), whereas no change in relative hip angle was evident (P = .10). Absolute angle of the trunk increased with time (P < .001), whereas the absolute angle of the shank decreased (P < .001). In response to the fatiguing task, subjects reduced muscle activation and force production and altered jumping technique; however, these changes were not dependent on gender.

Reduced muscle activation during exercise related to brain oxygenation and metabolism in humans

Maximal exercise may be limited by central fatigue defined as an inability of the central nervous system to fully recruit the involved muscles. This study evaluated whether a reduction in the cerebral oxygen-to-carbohydrate index (OCI) and in the cerebral mitochondrial oxygen tension relate to the ability to generate a maximal voluntary contraction and to the transcranial magnetic stimulated force generation. To determine the role of a reduced OCI and in central fatigue, 16 males performed low intensity, maximal intensity and hypoxic cycling exercise. Exercise fatigue was evaluated by ratings of perceived exertion (RPE), arm maximal voluntary force (MVC), and voluntary activation of elbow flexor muscles assessed with transcranial magnetic stimulation. Low intensity exercise did not produce any indication of central fatigue or marked cerebral metabolic deviations. Exercise in hypoxia (0.10) reduced cerebral oxygen delivery 25% and decreased 11+/-4 mmHg (P<0.001) together with OCI (6.2+/-0.7 to 4.8+/-0.5, P<0.001). RPE increased while MVC and voluntary activation were reduced (P<0.05). During maximal exercise declined 8+/-4 mmHg (P<0.05) and OCI to 3.8+/-0.5 (P<0.001). RPE was 18.5, and MVC and voluntary activation were reduced (P<0.05). We observed no signs of muscular fatigue in the elbow flexors and all control MVCs were similar to resting values. Exhaustive exercise provoked cerebral deoxygenation, metabolic changes and indices of fatigue similar to those observed during exercise in hypoxia indicating that reduced cerebral oxygenation may play a role in the development of central fatigue and may be an exercise capacity limiting factor.

The cost of walking downhill: Is the preferred gait energetically optimal?

Humans tend to prefer walking patterns that minimize energetic cost, but must also maintain stability to avoid falling over. The relative importance of these two goals in determining the preferred gait pattern is not currently clear. We investigated the relationship between energetic cost and stability during downhill walking, a context in which gravitational energy will assist propulsion but may also reduce stability. We hypothesized that humans will not minimize energetic cost when walking downhill, but will instead prefer a gait pattern that increases stability. Simulations of a dynamic walking model were used to determine whether stable downhill gaits could be achieved using a simple control strategy. Experimentally, twelve healthy subjects walked downhill at 1.25 m/s (0, 0.05, 0.10, and 0.15 gradients). For each slope, subjects performed normal and relaxed trials, in which they were instructed to reduce muscle activity and allow gravity to maximally assist their gait. We quantified energetic cost, stride timing, and leg muscle activity. In our model simulations, increase in slope reduced the required actuation but also decreased stability. Experimental subjects behaved more like the model when using the relaxed rather than the normal walking strategy; the relaxed strategy decreased energetic cost at the steeper slopes but increased stride period variability, an indicator of instability. These results indicate that subjects do not take optimal advantage of the propulsion provided by gravity to decrease energetic cost, but instead prefer a more stable and more costly gait pattern.