Reduced Malondialdehyde Levels Research Articles

Suppression of inflammation in ulcerative colitis rats by avocado and pomegranate

BackgroundPomegranate seed oil (PSO) and avocado seed oil (ASO) are natural polyphenols with established anti-inflammatory activity. PurposeThis study aimed to investigate the molecular mechanisms underlying the therapeutic efficacy of PSO and ASO in experimental ulcerative colitis (UC) with reference to sulfasalazine (SLZ). MethodsEighty male albino rats were divided equally into 8 groups; Normal, PSO, ASO, SLZ, UC-control, (UC+PSO), (UC+ASO) and (UC+SLZ) groups. Colitis was induced by intra-rectal injection of acetic acid. PSO (0.5ml/ 200g), ASO (1ml/ 250g) and SLZ (100mg/kg) were administered orally once/day for 14 days, 24h after colitis induction. Colitis was evaluated by measuring disease activity index (DAI), colon weight/length ratio and histologic inflammatory score. Vascular endothelial growth factor receptor-2 (VEGFR-2), colonic macrophage migration inhibitory factor (MIF), and malondialdehyde (MDA) were determined. Colonic gene expression of TNF-α, VEGF and heme oxygenase-1 (HO-1) were also estimated. ResultsPSO and ASO treatments to UC rats significantly reduced DAI, weight/length ratio, VEGFR-2, and colon histologic inflammatory score versus UC-controls. ASO significantly suppressed MIF levels and TNF-α expression greater than PSO. However, PSO was more significant than ASO in reducing MDA levels and up-regulating HO-1 expression. Both oils significantly down-regulated VEGF expression. The obtained biochemical and histological changes induced by UC were nearly corrected by SLZ. ConclusionThe proved beneficial effect of PSO and ASO as anti-inflammatory, anti-angiogenic, and antioxidant in UC rats could be mediated by suppression of TNF-α, VEGF, and MIF and up-regulation of HO-1.

Protective effects of sugarcane polyphenol against UV-B-induced photoaging in Balb/c mouse skin: Antioxidant, anti-inflammatory, and anti-glycosylation Effects.

Although the benefits of sugarcane polyphenol (SP) are well documented, its function in preventing photoaging has not yet been investigated. This study aimed to investigate the protective effects of SP in preventing ultraviolet (UV)-B-induced skin photoaging in Balb/c mice, as well as the underlying mechanism. Chlorogenic acid was determined to be the primary component of SP by using high-performance liquid chromatography-mass spectrometry. SP and chlorogenic acid were orally administrated to mice for 56 days, and UV-B radiation exposure was administered 14 days after SP and chlorogenic acid administration and lasted 42 days to cause photoaging. SP and chlorogenic acid administrations significantly alleviated the UV-B-induced mouse skin photoaging, as indicated by the decrease in epidermal thickness, increase in the collagen (COL) volume fraction, and elevation in type 1 and type 3 COL contents. Notably, both SP and chlorogenic acid effectively reversed the overexpression of matrix metalloproteinase induced by UV-B exposure in the mouse skin. Furthermore, SP and chlorogenic acid reduced the expression of receptor for advanced glycosylation end products in the mice; amplified the activities of antioxidant enzymes superoxide dismutase and catalase; reduced malondialdehyde levels; and decreased inflammatory cytokines interleukin 1β, interleukin 6, and tumor necrosis factor α levels. SP could be a prospective dietary supplement for anti-photoaging applications due to its antioxidant, anti-inflammatory, and anti-glycosylation attributes, and chlorogenic acid might play a major role in these effects. PRACTICAL APPLICATION: This study can provide a scientific basis for the practical application of sugarcane polyphenols. We expect that sugarcane polyphenols can be used in food and beverage products to provide flavor while combating skin aging.

The potential effect of temulawak (Curcuma xanthorrhiza Roxb.) and garlic (Allium sativum L.) as a radioprotective agent against 6 Gy total body irradiation in rats

The main impact of gamma radiation on a biological system is the formation of Reactive Oxygen Species (ROS) and free radicals. The study aimed to explore the potential effect of temulawak (Curcuma xanthorrhiza Roxb.) and garlic (Allium sativum L.) against 6 Gy total body irradiation (TBI) in rats by observing malondialdehyde (MDA), glutathione (GSH) and comet assay. Twenty male rats were divided into five groups: control, 6 Gy, temulawak extract + 6 Gy, garlic extract + 6 Gy, and n-acetyl cysteine (NAC) + 6 Gy. MDA and GSH were measured on liver and spleen tissue homogenates, while comet assay was on lymphocyte cells. Gamma irradiation at 6 Gy significantly increased the MDA level and comet assay compared to the control group, while the GSH level decreased (p < 0.05). Temulawak extract significantly reduced MDA levels and comet assay compared to the 6 Gy group while increasing GSH levels in the liver. Garlic extract significantly drops comet assay while increasing GSH levels in the liver. NAC decreases MDA levels in the liver and comet assay while increasing GSH levels in the spleen. It could be concluded that temulawak extract has a better radioprotective agent than garlic extract and is almost identical to NAC as a standard antioxidant.

Efek Ekstrak Ampas Seduhan Bubuk Kopi Dalam Menghambat Produksi Malondialdehid Pada Isolat Monosit Perifer Manusia yang Distimuli Dengan Eksotoksin Streptococcus mutans

Monocytes as non-specific immune cells provide an early response to pathogens and foreign objects that enter the body. When exposed to pathogens or foreign objects, monocytes experience an increase in the number of free radicals. Free radicals activate phospholipase enzymes that break down membrane phospholipids into phosphate, glycerol and fatty acids. Unsaturated fatty acids (PUFAs) react with free radicals to undergo peroxidation to produce malondialdehyde (MDA). It takes antioxidant compounds that can reduce excess free radicals in the body. Coffee grounds as solid waste from the consumption of processed coffee products are not used and disposed of, it is suspected that they can be used as antioxidants. This study aims to determine the effect of coffee grounds steeping antioxidants on the profile of MDA levels in monocytes stimulated by the S. mutans exotoxin (In Vitro). Research was done in experimental post test only control group design. The object of this study was human peripheral blood monocytes, which were isolated by the Gradient Density method. Following in vitro exposure to the exotoxin S. mutans, the monocyte isolates were subjected to treatment with brewed coffee grounds extract at three different concentrations (0.25 mg/ml, 0.50 mg/ml, and 0.75 mg/ml). The MDA levels were subsequently determined by employing the TBArs method on a UV-Vis spectrophotometer. The results of MDA levels were analyzed using One Way Anova and LSD. The MDA level data showed significance (p<0.05) with the average MDA level in the Negative Control group1.49 g/ml; Positive Control 10.62 g/ml; T1 8.15 g/ml; T2 6.59 g/ml; and T3 4.69 g/ml. It can be concluded that the extract of brewed coffee grounds can reducing MDA levels in monocytes exposed to the exotoxin of S. mutans.

Bioactivity of Papua Red Fruit Extract (Pandanus conoideus L.) Against Superoxide dismutase, Malondialdehyde and Blood Glucose of Rat (Rattus norvegicus L.) Hyperglycemia

Red fruit (Pandanus conodus L.) is a plant native to Papua, ethnomedical used as a traditional medicine with a wide spectrum of pharmacological effects. However, research on the bioactivity of red fruit is still very poorly reported. The aim of this study was to characterize the bioactive content, antioxidant bioactivity and the effect of lowering blood glucose in hyperglycemic rats. Red fruit has extracted used the maceration method with 95% ethanol. Analysis of active compound content using Gas Chromatography Mass Spectrophotometry (GC MS). Analysis of Malondialdehyde (MDA), Superoxide dismutase (SOD) and blood glucose levels was carried out using the in vivo method using white rats. The research results showed that red fruit ethanol extract contained 173 compounds from six main groups of compounds. Red fruit extract has effective in reducing MDA levels in hyperglycemic mice. K4 and K5 treatments were the best treatments for reducing MDA. Red fruit extract affects the duration of induction of increased SOD in hyperglycemic rats. Treatments K4 and K5 were the best treatments for SOD induction. Red fruit extract affects reducing blood glucose in hyperglycemic mice. Red fruit extract has antioxidant activity and lowers blood glucose. Red fruit extract has the potential to be developed as a source of bioactive antioxidants and antidiabetics.
 
 Keywords: Glucose, hyperglycemia, malondialdehyde, superoxide dismutase

Yarrowia lipolytica as a promising protein source for Pacific white shrimp (Litopenaeus vannamei) diet: impact on growth performance, metabolism, antioxidant capacity, and apparent digestibility

Exploring alternatives to fishmeal (FM) in aquaculture nutrition remains a hot topic in this area. Yarrowia lipolytica (YL), a promising single-cell protein source, has yet to be evaluated for its use on the Pacific white shrimp (Litopenaeus vannamei) diet. This investigation assessed the effects of YL on growth performance, metabolism, antioxidant capacity, and nutrient digestibility in shrimp. Seven diets with equal protein and lipid contents were prepared, incorporating gradient YL (dry matter basis): 0% (control), 1.50%, 3.00%, 4.50%, 6.00%, 9.00%, and 12.00%. During a 56-day feeding trial involving shrimp, with an initial average weight of 0.32 ± 0.02 g, each kind of diet was randomly assigned to six tanks. It was observed that the growth performance improved initially but then exhibited a declining trend as the dietary inclusion of YL increased. Regression analysis indicated 2.68% as the optimal YL percentage for a specific growth rate (SGR), accounting for 10.54% of the dietary FM content. A higher YL (over 6.00%), which is equivalent to replacing over 23.6% of the FM, led to increased feed intake and conversion ratios, possibly correlating with decreased YL digestibility. However, YL inclusion across the studied range had no detrimental impact on intestinal or hepatopancreatic morphology. Notably, higher YL levels significantly enhanced hepatopancreatic trypsin activity and reduced serum triglyceride and cholesterol levels. The inclusion of YL notably augmented antioxidant defense mechanisms in shrimp, as evidenced by increased total antioxidant capacity and catalase activities, as well as reduced malondialdehyde levels in the hepatopancreas, possibly due to the presence of β-glucan and mannan oligosaccharides in YL. These findings support YL being viable as a protein source in shrimp feeds.

SlWRKY81 regulates Spd synthesis and Na+/K+ homeostasis through interaction with SlJAZ1 mediated JA pathway to improve tomato saline-alkali resistance.

Saline-alkali stress is an important abiotic stress factor affecting tomato (Solanum lycopersicum L.) plant growth. Although the involvement of the tomato SlWRKY gene family in responses to saline-alkali stress has been well established, the mechanism underlying resistance to saline-alkali stress remains unclear. In this study, we investigated the role of SlWRKY81 in conferring saline-alkali stress resistance by using overexpression and knockout tomato seedlings obtained via genetic modification. We demonstrated that SlWRKY81 improves the ability of tomato to withstand saline-alkali stress by enhancing antioxidant capacity, root activity, and proline content while reducing malondialdehyde levels. Saline-alkali stress induces an increase in jasmonic acid (JA) content in tomato seedlings, and the SlWRKY81 promoter responds to JA signaling, leading to an increase in SlWRKY81 expression. Furthermore, the interaction between SlJAZ1 and SlWRKY81 represses the expression of SlWRKY81. SlWRKY81 binds to W-box motifs in the promoter regions of SlSPDS2 and SlNHX4, thereby positively regulating their expression. This regulation results in increased spermidine (Spd) content and enhanced potassium (K+) absorption and sodium (Na+) efflux, which contribute to the resistance of tomato to saline-alkali stress. However, JA and SlJAZ1 exhibit antagonistic effects. Elevated JA content reduces the inhibitory effect of SlJAZ1 on SlWRKY81, leading to the release of additional SlWRKY81 protein and further augmenting the resistance of tomato to saline-alkali stress. In summary, the modulation of Spd synthesis and Na+/K+ homeostasis mediated by the interaction between SlWRKY81 and SlJAZ1 represents a novel pathway underlying tomato response to saline-alkali stress.

Yunvjian decoction mitigates hyperglycemia in rats induced by a high-fat diet and streptozotocin via reducing oxidative stress in pancreatic beta cells

Ethnopharmacological relevanceYunvjian (YNJ), a traditional Chinese herbal formula first reported in Jing Yue Quan Shu, is commonly used in the clinical treatment of type 2 diabetes mellitus (T2DM). However, the mechanism by which YNJ affects T2DM remains unclear. Aim of the studyThis study aimed to assess the therapeutic effects of YNJ on T2DM and explore the potential mechanism involved. Materials and methodsHigh-performance liquid chromatography (HPLC) was used to identify the chemical compounds of YNJ. The anti-T2DM effects of YNJ were observed in a high-fat diet/streptozotocin induced rat model. The type 2 diabetic rats were prepared as follows: rats were fed a high-fat diet for four weeks and then intraperitoneally injected with a low dose (30 mg/kg) of streptozotocin. YNJ and the positive control metformin were used in these experiments. Biochemical assays were implemented to determine the fasting blood glucose, glucose tolerance, insulin sensitivity, serum lipid levels, and oxidative stress index of the pancreas. Hematoxylin-eosin (H&E) staining was used to assess histopathological alterations in the pancreas. The mechanism by which YNJ affects T2DM was evaluated in INS-1 cells treated with glucose and high sodium palmitate. YNJ-supplemented serum was used in these experiments. Methyl thiazolyl tetrazolium assays, enzyme-linked immunosorbent assays, Nile red staining, flow cytometric analysis, and Western blotting were used to assess apoptosis, insulin secretion, lipid accumulation, reactive oxygen species production, and protein levels. ResultsFive major compounds were identified in YNJ. In high-fat diet/streptozotocin-induced diabetic rats, YNJ-M notably decreased fasting blood glucose and lipid levels; ameliorated glucose tolerance, insulin sensitivity, and islet morphology; reduced Malondialdehyde levels; and restored superoxide dismutase activity in the pancreatic islets. Furthermore, the effect of YNJ-M was significantly greater than that of YNJ-L, and YNJ-H had little effect on diabetic rats. In vitro experiments revealed that YNJ-supplemented serum (10%, 15%, and 20%) dramatically suppressed apoptosis, mitigated intracellular lipid accumulation and reduced intracellular oxidative stress levels in a dose-dependent manner. Additionally, YNJ-supplemented serum increased the protein expression of Nuclear factor erythroid 2-related factor 2, Heme oxygenase-1, and superoxide dismutase 1 and inhibited the protein expression of Kelch-like ECH-associated protein 1. ConclusionYNJ ameliorates high-fat diet/streptozotocin induced experimental T2DM. The underlying mechanism involves reducing oxidative stress in pancreatic beta cells. The findings of this study provide scientific justification for the application of the traditional medicine YNJ in treating T2DM.

Long-term administration of omeprazole in mice: a study of behavior, inflammatory, and oxidative stress alterations with focus on central nervous system.

Chronic use of omeprazole has been linked to central effects alongside with the global concern of increasing appearance of neuropsychiatric disorders. This study aimed to identifying behavioral, inflammatory, and oxidative stress alterations after long-term administration of omeprazole. C57BL/6 mice were divided in groups: OME and Sham, each received either solutions of omeprazole or vehicle, administered for 28 days by gavage. Results observed in the omeprazole-treated mice: Decrease in the crossing parameter in the open field, no change in the motor performance assessed by rotarod, an immobility time reduction in the forced swimming test, improved percentage of correct alternances in the Ymaze and an exploration time of the novel object reduction in the novel object recognition. Furthermore, a reduced weight gain and hippocampal weight were observed. There was an increase in the cytokine IL1-β levels in both prefrontal cortex (PFC) and serum, whereas TNF-α increased only in the PFC. Nitrite levels increased in the hippocampus (HP) and PFC, while malondialdehyde (MDA) and glutathione (GSH) levels decreased. These findings suggest that omeprazole improves depressive-like behavior and working memory, likely through the increase in nitrite and reduction in MDA levels in PFC and HP, whereas, the impairment of the recognition memory is more likely to be related to the reduced hippocampal weight. The diminished weight gain might be associated with the IL-1β increased levels in the peripheral blood. Altogether, omeprazole showed to have the potential to impact at central level and inflammatory and oxidative parameters might exert a role between it.

Protective Effects of Alpha-lipoic Acid, Resveratrol, and Apigenin Against Oxidative Damages, Histopathological Changes, and Mortality Induced by Lung Irradiation in Rats.

This study investigated the protective effects of three antioxidants on radiationinduced lung injury. Oxidative stress is one of the key outcomes of radiotherapy in normal tissues. It can induce severe injuries in lung tissue, which may lead to pneumonitis and fibrosis. Recently, interest in natural chemicals as possible radioprotectors has increased due to their reduced toxicity, cheaper price, and other advantages. The present study was undertaken to evaluate the radioprotective effect of Alpha-lipoic Acid (LA), Resveratrol (RVT), and Apigenin (APG) against histopathological changes and oxidative damage and survival induced by ionizing radiation (IR) in the lung tissues of rats. First, the lung tissue of 50 mature male Wistar rats underwent an 18 Gy gamma irradiation. Next, the rats were sacrificed and transverse sections were obtained from the lung tissues and stained with hematoxylin and eosin (H and E) and Mason trichrome (MTC) for histopathological evaluation. Then, the activity of Glutathione peroxidase (GPx), Superoxide Dismutase (SOD), and Malondialdehyde (MDA) was measured by an ELISA reader at 340, 405, and 550 nm. Based on the results of this study, IR led to a remarkable increase in morphological changes in the lung. However, APG, RVT, and LA could ameliorate the deleterious effects of IR in lung tissue. IR causes an increase in GPX level, and APG+IR administration causes a decrease in the level of GPX compared to the control group. Also, the results of this study showed that RVT has significant effects in reducing MDA levels in the short term. In addition, compared to the control group, IR and RVT+IR decrease the activity of SOD in the long term in the lung tissues of rats. Also, the analysis of results showed that weight changes in IR, LA+IR, APG+IR, and control groups were statistically significant. APG and RVT could prevent tissue damage induced by radiation effects in rat lung tissues. Hence, APG, LA, and RVT could provide a novel preventive action with their potential antioxidant anti-inflammatory properties, as well as their great safety characteristic.

Propionyl-l-carnitine mitigates ischemia-reperfusion injury in rat epigastric island flaps

BackgroundIschemia-reperfusion injury presents a substantial concern in various medical scenarios, notably in reconstructive surgery involving tissue flaps. Despite reports on the protective benefits of Propionyl-l-carnitine against ischemia-reperfusion injury, a thorough assessment of its efficacy in epigastric island flap models is currently lacking. MethodsSixteen male Sprague-Dawley rats underwent epigastric island flap surgery and were divided into two groups: a Propionyl-l-carnitine group that received intraperitoneal Propionyl-l-carnitine prior to ischemia induction and a sham group that received saline treatment. A comprehensive evaluation was performed including macroscopic, biochemical and histological assessments encompassing measurements of flap survival areas, lipid peroxidation (malondialdehyde), glutathione, myeloperoxidase, nitric oxide and peripheral neutrophil counts. ResultsThe Propionyl-l-carnitine group demonstrated significantly increased flap survival areas when compared to the sham group. Administration of Propionyl-l-carnitine led to reduced malondialdehyde levels and elevated glutathione levels indicating a reduction in oxidative stress. Furthermore, the Propionyl-l-carnitine group exhibited lower myeloperoxidase levels, higher nitric oxide levels and reduced peripheral neutrophil counts, suggesting a decrease in the inflammatory response. Histopathological analysis revealed decreased levels of inflammation, necrosis, polymorphonuclear leukocyte infiltration and edema in the Propionyl-l-carnitine group. Additionally, vascularity was enhanced in the Propionyl-l-carnitine group. ConclusionThis study provides compelling evidence that Propionyl-l-carnitine administration effectively mitigates the deleterious effects of ischemia-reperfusion injury in epigastric island flaps. This is substantiated by the improved flap survival, diminished oxidative stress and inflammation, as well as the enhanced vascularity observed. Propionyl-l-carnitine emerges as a promising therapeutic intervention to enhance tissue flap survival in reconstructive surgery, warranting further exploration through larger-scale investigations.

Effect of inclusion of zinc-glycine chelate and zinc sulphate on live performance, immunity and lipid peroxidation in broilers

ABSTRACT Zinc (Zn) is a biologically significant trace mineral, serving as a cofactor in various metabolic functions. This study aimed to assess the relative effectiveness of organic and inorganic Zn on the growth performance, lipid peroxidation and humoral immune response in broiler chickens (Ross 308). A total of 450 d-old broilers were divided into five groups: the control group, a group receiving a basal diet with 50 mg/kg organic Zn (T1), another with 60 mg/kg organic Zn (T2), and two groups with basal diets supplemented with inorganic Zn at rates of 50 and 60 mg/kg zinc sulfate for the fourth (T3) and fifth (T4) groups, respectively. The experiment spanned 35 days. Results indicated that zootechnical characteristics such as weekly weight gain, feed intake, feed conversion ratio and dressing percentage were significantly higher (P < 0.05) in T1 compared to both the control and the inorganic Zn supplementation groups. Additionally, the antibody titre against Newcastle disease was significantly (P < 0.05) elevated, while malondialdehyde (MDA) levels were significantly (P < 0.05) lower in T1 and T2 compared to the control and the inorganic Zn supplementation groups. In conclusion, broilers supplemented with 50 mg/kg organic Zn exhibited significantly improved growth performance, reduced MDA levels, and enhanced humoral immune response.

Optimization of Microwave-Assisted Extraction Process of Total Flavonoids from Salicornia bigelovii Torr. and Its Hepatoprotective Effect on Alcoholic Liver Injury Mice.

The objective of this study was to determine the optimal extraction conditions for total flavonoids from S. bigelovii using microwave-assisted extraction and to analyze the protective effect of total flavonoids from S. bigelovii on alcoholic liver injury in mice. The optimization of the process conditions for the microwave-assisted extraction of total flavonoids from S. bigelovii was performed using response surface methodology, and an alcohol-induced acute liver injury model in mice was used to investigate the effects of different doses of total flavonoids (100 mg/kg, 200 mg/kg, and 400 mg/kg) on the levels and activities of serum alanine aminotransferase kits (ALT), glutamic oxaloacetic transaminase kits (AST), superoxide dismutase kits (SOD), glutathione peroxidase kits (GSH-Px), and malondialdehyde (MDA). We performed hematoxylin-eosin (H&E) staining analysis on pathological sections of mouse liver tissue, and qRT-PCR technology was used to detect the expression levels of the inflammatory factors IL-1 β, IL-6, and TNF-α. The results revealed that the optimal extraction process conditions for total flavonoids in S. bigelovii were a material-to-liquid ratio of 1:30 (g/mL), an ethanol concentration of 60%, an extraction temperature of 50 °C, an ultrasound power of 250 W, and a yield of 5.71 ± 0.28 mg/g. Previous studies have demonstrated that the flavonoids of S. bigelovii can significantly inhibit the levels of ALT and AST in the serum (p < 0.001), reduce MDA levels (p < 0.001), increase the activity of the antioxidant enzymes SOD and GSH-Px (p < 0.001), and inhibit the IL-1 β, IL-6, and TNF-α gene expression levels (p < 0.001) of inflammatory factors. The total flavonoids of S. bigelovii exert a protective effect against alcoholic liver injury by reducing the levels of inflammation, oxidative stress, and lipid peroxidation caused by alcohol. The results of this study lay the foundation for the high-value utilization of S. bigelovii and provide new resources for the development of liver-protective drugs.

Impact of Mar’ke Bilar Healthy Drink on Blood Levels of Malondialdehyde, Superoxide Dismutase, and Hemoglobin in Soccer Athletes at PPLP North Sumatra

Background: High-intensity soccer activities due to overtraining can cause the emergence of reactive oxygen species (ROS), resulting in an imbalance of free radicals in the form of malondialdehyde (MDA) and endogenous antioxidants in the form of superoxide dismutase (SOD). As a result, it can interfere with the formation of hemoglobin (Hb) needed by an athlete to take in oxygen (VO2max). Objectives: This study was designed to determine the effect of giving a healthy drink Mar’ke Bilar on MDA, SOD, and Hb levels in soccer athletes at PPLP North Sumatra, Indonesia. Methods: This type of research is quasi-experimental with a one-group pre- and post-test design. The population and research samples amounted to 33 soccer athletes at PPLP North Sumatra. The administration of Mar’ke Bilar as much as 200 ml was carried out for 14 days, and then MDA, SOD, and Hb levels were measured before and after. A normality test was conducted with the Kolmogorov-Smirnov test, followed by a different test using a paired t-test. Results: Malondialdehyde levels decreased from 17.5 to 10.9 µmol/L. There was an increase in SOD from 45.945 to 53.256 pg/mL. However, the results of Hb also showed an increase from 14.3 to 15.1 gr/dL. There was a significant effect on levels of MDA (P = 0.001), SOD (P = 0.001), and Hb (P = 0.002). Conclusions: Mar’ke Bilar healthy drink consumption has an effect on reduced MDA levels and increased SOD and Hb.

Neferine inhibits the progression of diabetic nephropathy by modulating the miR-17-5p/nuclear factor E2-related factor 2 axis.

To investigate the effect of Neferine (Nef) on diabetic nephropathy (DN) and to explore the mechanism of Nef in DN based on miRNA regulation theory. A DN mouse model was constructed and treated with Nef. Serum creatinine (Crea), blood urea (UREA) and urinary albumin were measured in mice by kits, and renal histopathological changes and fibrosis were observed by hematoxylin-eosin staining and Masson staining. Renal tissue superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) activities were measured by enzyme-linked immunosorbent assay (ELISA). Western blotting was used to detect the expression of nuclear factor E2-related factor 2 (Nrf2)/ heme oxygenase 1 (HO-1) signaling pathway-related proteins in kidney tissues. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to detect the expression of miR-17-5p in kidney tissues. Subsequently, a DN in vitro model was constructed by high glucose culture of human mesangial cells (HMCs), cells were transfected with miR-17-5p mimic and/or treated with Nef, and we used qRT-PCR to detect cellular miR-17 expression, flow cytometry to detect apoptosis, ELISAs to detect cellular SOD, MDA, and GSH-Px activities, Western blots to detect Nrf2/HO-1 signaling pathway-related protein expression, and dual luciferase reporter gene assays to verify the targeting relationship between Nrf2 and miR-17-5p. Administration of Nef significantly reduced the levels of blood glucose, Crea, and UREA and the expression of miR-17-5p, improved renal histopathology and fibrosis, significantly reduced MDA levels, elevated SOD and GSH-Px activities, and activated Nrf2 expression in kidney tissues from mice with DN. Nrf2 is a post-transcriptional target of miR-17-5p. In HMCs transfected with miR-17-5p mimics, the mRNA and protein levels of Nrf2 were significantly suppressed. Furthermore, miR-17-5p overexpression and Nef intervention resulted in a significant increase in high glucose-induced apoptosis and MDA levels in HMCs and a significant decrease in the protein expression of HO-1 and Nrf2. Collectively, these results indicate that Nef has an ameliorative effect on DN, and the mechanism may be through the miR-17-5p/Nrf2 pathway.

Effect of hydro-alcoholic extract of Nigella sativa on cisplatin-induced memory impairment and brain oxidative stress status in male rats.

Studies have shown the complications of chemotherapy on learning and memory. Empirical evidence suggests that Nigella sativa (NS) has neuroprotective activities. Therefore, the aim of our study was to investigate the effects of NS on cisplatin-induced memory impairment. This study was conducted on 40 male rats grouped as: control (saline: 2 ml/kg, intraperitoneally (IP), once weekly/2 weeks), cisplatin (Cis, 2 mg/kg, IP, once weekly/2 weeks), NS (200 mg/kg, IP, once weekly/2 weeks), Cis +NS 200 (2 mg/kg Cis + 200 mg/kg NS, IP, once weekly/2 weeks), and Cis +NS 400 (2 mg/kg Cis + 400 mg/kg NS, IP, once weekly/2 weeks). Morris water maze (MWM) test was used to assess spatial learning and memory. In addition, superoxide dismutase (SOD) activity, and thiol and malondialdehyde (MDA) levels were evaluated in the brain. Cis significantly enhanced the traveled distance and time spent in the target quadrant in the MWM test. Additionally, MDA levels increased in the Cis group, while thiol and SOD decreased in this group. As a result of treatment with NS, behavioral results were reversed in the groups receiving NS compared to the Cis group. Also, NS reduced MDA level but improved SOD and thiol levels in brain tissue samples. NS could improve memory impairment and oxidative stress in animals receiving Cis. Therefore, NS could be used as a potential food supplement to prevent neurotoxicity in patients undergoing chemotherapy.

Promising Eco-Friendly Nanoparticles for Managing Bottom Rot Disease in Lettuce (Lactuca sativa var. longifolia)

Developing innovative, eco-friendly fungicide alternatives is crucial to mitigate the substantial threat fungal pathogens pose to crop yields. In this study, we assessed the in vitro effectiveness of SiO2, CuO, and γFe2O3 nanoparticles against Rhizoctonia solani. Furthermore, greenhouse experiments were conducted in artificially infested soil to evaluate the in vivo impact of nanoparticles under study. Two application methods were employed: soil drenching with 10 mL per pot at concentrations of 50, 100, and 200 mg L−1, and seedling dipping in nanoparticle suspensions at each concentration combined with soil drench. The combined treatment of 200 mg L−1 γFe2O3 or CuO nanoparticles showed the highest in vitro antifungal activity. Conversely, SiO2 nanoparticles demonstrated the lowest in vitro activity. Notably, the application of 200 mg/L SiO2 via the dipping and soil drenching methods decreased counts of silicate-solubilizing bacteria and Azospirillum spp. Whereas, application of 100 mg L−1 γFe2O3 nanoparticles via soil drenching increased soil bacterial counts, and CuO nanoparticles at 50 mg L−1 through dipping and soil drenching had the highest dehydrogenase value. γFe2O3 nanoparticles improved plant photosynthetic pigments, reduced malondialdehyde levels, and minimized membrane leakage in lettuce plants. A root anatomical study showed that 200 mg L−1 CuO nanoparticles induced toxicity, whereas 200 mg L−1 γFe2O3 or SiO2 nanoparticles positively affected root diameter, tissue structure, and various anatomical measurements in lettuce roots. γFe2O3 nanoparticles hold promise as a sustainable alternative for managing crop diseases.

Efficacy of tanshinone IIA in rat models with myocardial ischemia-reperfusion injury: a systematic mini-review and meta-analysis.

Myocardial ischemia-reperfusion injury (MIRI) refers to severe damage to the ischemic myocardium following the restoration of blood flow, and it is a major complication of reperfusion therapy for myocardial infarction. Notably, drugs such as metoprolol have been utilized to reduce ischemia-reperfusion injury. Tanshinone IIA is a major constituent extracted from Salvia miltiorrhiza Bunge. Recently, tanshinone IIA has been studied extensively in animal models for controlling MIRI. Therefore, we conducted a meta-analysis on the application of tanshinone IIA in rat models with MIRI to evaluate the therapeutic effects of tanshinone IIA. A comprehensive search was conducted across PubMed, Web of Science, Embase, the Cochrane Library, the China National Knowledge Infrastructure database, the Wanfang database, and the Chinese Scientific Journal Database to gather studies on tanshinone IIA intervention in rat models with MIRI.We employed SYRCLE's risk of bias tool to assess study quality. The primary outcome indicators were superoxide dismutase (SOD) and malondialdehyde (MDA). Myocardial infarction area was a secondary outcome indicator. This study was registered at PROSPERO (registration number CRD 42022344447). According to the inclusion and exclusion criteria, 15 eligible studies were selected from 295 initially identified studies. In rat models with MIRI, tanshinone IIA significantly increased SOD levels while reducing MDA levels and myocardial infarction area. Moreover, the duration of myocardial ischemia influenced the effectiveness of tanshinone IIA. However, additional high-quality research studies are needed to establish the efficacy and definitive guidelines for the use of tanshinone IIA. Animal studies demonstrated that tanshinone IIA exerted a significant therapeutic effect when the ischemia duration was less than 40 minutes. Tanshinone IIA was found to be more effective when administered via intravenous, intraperitoneal, and intragastric routes at doses above 5 mg/kg. Additionally, treatment with tanshinone IIA at all stages-prior to myocardial ischemia, after ischemia but before reperfusion, prior to ischemia and after reperfusion, and after reperfusion-showed satisfactory results. Tanshinone IIA enhanced SOD activity and reduced MDA levels, thereby ameliorating oxidative stress damage during MIRI. Additionally, it reduced the myocardial infarction area, indicating its effectiveness in mitigating MIRI-induced damage in rats and demonstrating a myocardial protective effect. These findings contribute valuable insights for developing MIRI treatment strategies.

Effects of supplementation with mature coconut water on oxidative stress, semen quality, reproductive hormone levels, and sexual behavior in Boer bucks

AbstractMature coconut water (MCW) has been demonstrated to contain bioactive compounds with antioxidant properties. In vivo research showed that MCW supplementation increased semen quality in rats, suggesting that it may boost reproductive performance. This study investigated the impact of MCW on the reproduction of Boer bucks. Two groups of 12 sexually mature bucks were given either plain water (control) or MCW at 5 mL/kg of body weight daily for 60 days. Sexual behaviors were studied using the focal observation technique, whereas semen was collected for quality assessment. Oxidative stress markers, namely, malondialdehyde (MDA) and glutathione (GSH), along with reproductive hormones, specifically luteinizing hormone (LH), follicle‐stimulating hormone (FSH), and testosterone, were quantified in blood serum samples via enzyme‐linked immunosorbent assay (ELISA). The oxidative stress analysis showed elevated GSH and reduced MDA levels, accompanied by enhanced sperm quality, including superior motility, concentration, viability, and fewer morphological abnormalities (p &lt; 0.05), in the group receiving MCW. Moreover, there was a rise in LH, FSH, and testosterone levels with improved sexual behaviors (p &lt; 0.05), including a reduced latency to the first mount, increased mount attempts, higher libido, and prolonged kicking and sniffing durations. In conclusion, MCW may improve reproductive health and fertility in Boer bucks.

Ameliorative effect of Vitis gracilis leaf decoction against sensory and motoric disorders, oxidative stress, and cerebellar degeneration in diabetic mice

Vitis gracilis (Vitaceae) is a potent medicinal plant, but its effectiveness in counteracting neuronal issues due to diabetes is uncertain. This study aimed to investigate whether the leaf decoction of V. gracilis could manage sensory and motoric disorders, oxidative stress, and cerebellar degeneration caused by diabetes mellitus. Twenty-five adult male mice were divided into five treatment groups: non-diabetic (non-DM), diabetic (alloxan-induced diabetes mellitus; DM), and DM treated with V. gracilis leaf decoction at doses of 25 g/l (DM + 25 g/l VgD), 50 g/l (DM + 50 g/l VgD), or 100 g/l (DM + 100 g/l VgD). The decoction was administered orally for 30 days. The results showed that V. gracilis leaf decoction did not significantly lower blood glucose levels or improve insulin tolerance in diabetic mice (p &gt; 0.05). However, it substantially maintained sensory response and motoric balance and reduced malondialdehyde levels in brain tissue, especially at a 100 g/l dose (p &lt; 0.05). Moreover, higher decoction doses alleviated histopathological alterations in the cerebellum by significantly preserving molecular and granular layer thickness while reducing degenerated Purkinje cells (p &lt; 0.05). In conclusion, despite its inability to manage hyperglycemia, the decoction effectively improved sensory and motoric function, reduced oxidative stress, and mitigated cerebellar histopathological changes. Therefore, V. gracilis could be an alternative treatment for diabetes-related neurological disorders.