Alectoris Rufa Research Articles

Haemoparasites in red-legged partridge (Alectoris rufa): first record of Haemoproteus sp. in Italy?

During a health survey in the Province of Alessandria (NW Italy) 267 free ranging red-legged partridge (Alectoris rufa; 137 males and 130 females) were captured in 2009 (n = 101), 2010 (n = 130) and 2011 (n = 36). After biometric data and blood samples collection, animals were released on site. Blood samples were used for the preparation of blood smears. A total of 1.5% of smears presented parasites with a light blue finely granular cytoplasm in red blood cells. The parasites were identified, on the basis of their morphology (no molecular identification was possible), as being Haemoproteus sp. juvenile forms. The infestation prevalence was 0.8% in 2010 and 8.3% in 2011. No parasite was observed in 2009. The size of the infested erythrocytes was not altered (P > 0.05) and the nuclear displacement ratio was 0.8 ± 0.2. All the birds were apparently in good health status. To our knowledge this is the first description of Haemoproteus sp. in red-legged partridge, and more in general in birds belonging to Galliformes Order in Italy.

Are population changes of endangered little bustards associated with releases of red-legged partridges for hunting? A large-scale study from central Spain

The release of farm-reared game birds for hunting is an increasingly common game management practice. However, releasing could have negative effects on sympatric wild species, for example, through parasite transmission. Here, we document the spatial-temporal patterns and intensity of red-legged partridge releases in the province of Ciudad Real, Spain, over a 15-year period (2002–2016), relating them to local changes in the abundance of little bustards estimated from two surveys carried out in 2005 and 2016. Within the province, > 600,000 red-legged partridges were released annually over at least 20% of the area. Releasing intensity varied between estates and fluctuated over the 15-year study period, probably because of an economic crisis during 2008–2014. Overall, numbers of little bustards dropped by 46% between surveys, the decrease being more marked in the west of the province. Contrary to expectation, the only hunting estates where little bustards did not decrease were those with higher release intensity. This may be a consequence of management measures or other factors that benefit little bustards and are more prevalent on those estates than elsewhere, such as game crop provision, predator control or habitat quality.

Reducing nest predation of ground-nesting birds through conditioned food aversion

Populations of many ground-nesting bird species have declined substantially due to several factors, and predation can be a leading contributor to these declines. As a method for reducing the nest predation on ground-nesting birds, we tested whether conditioned food aversion (CFA) can reduce red-legged partridge (Alectoris rufa) nest predation by red foxes (Vulpes vulpes). With a before-after control-impact design we deployed 1329 artificial nests in two different habitats in Central Spain using thiram as an aversive. Artificial nests were monitored by camera traps to identify the red fox individuals during the predation events. 26 foxes were GPS-tagged to monitor their spatial movements and feeding behavior. Partridge density and productivity were monitored to assess the thiram treatment effect on partridge population. Thiram treatment decreased artificial nest predation by foxes in both study sites by 26.8–50.1%, but this was compensated by an increased predation by other predators, possibly enhanced by the availability of our artificial nests. 78% of identified foxes that ingested thiram-treated eggs, stopped nest predation after treatment. Foxes maintained stable territories during the whole study period. Partridge productivity was 132–677% higher in thiram-treatment areas than in control areas, and partridge density after treatment increased more in thiram-treated areas (193–292%) than in control areas (1.8–99%). Our study shows that CFA reduced ground nest predation by foxes, and had a positive effect on the partridge population despite the compensatory predation. This method could be used as a non-lethal tool for conservation of endangered ground-nesting bird species.

Non-native and hybrid in a changing environment: conservation perspectives for the last Italian red-legged partridge (Alectoris rufa) population with long natural history

The ever-increasing biotic homogenization - especially when associated with introgressive hybridization - raises concern for the reduction of the spatial component of diversity in wildlife worldwide. Nonetheless, there is a growing attention to the potential conservation value of hybridization in fastening the adaptive evolutionary responses to rapidly changing selective pressures. Under these premises, we investigated the genetic affinity of the red-legged partridge (Alectoris rufa) population inhabiting Elba Island (Italy) in the context of the overall species phylogeography and particularly of the nominate subspecies it is traditionally ascribed to. Although notoriously hybrid with the congeneric A. chukar, this island population is of undisputedly value because of its long natural history and self-sustainability. As such, its adaptive conservation management calls for a comprehensive knowledge including the assessment of its geographic origin. For this purpose, 110 fecal samples were collected across Elba, genotyped at their joint Cytochrome-b and Control Region genes (2,249 characters), and compared with 149 conspecifics from all over the species distribution range. We confirmed a widespread A. chukar mitochondrial DNA introgression in Elba partridges, whereas their expected formal assignment to the nominate subspecies from Italy and France was rejected, since these turned out to be closely related to conspecifics from the Iberian Peninsula. This counterintuitive result found support in a large variety of literary sources and compelling evidences from personal testimonies revealing recent intense management with farm-reared birds of Spanish origin. Although the nativeness of Elba partridges was disproved, we advise local authorities to keep warranting the ongoing conservation efforts - and especially restore the connectivity between the western and eastern island sub-populations - as this resource may still be conceived as the ultimate repository for part of the otherwise extinct Italian A. r. rufa genome. Indeed, the admixture with conspecifics from the Iberian Peninsula does not necessarily mean that the entirety of the native nuclear genome of Elba partridges has been wiped out. Furthermore, these latter represent an interesting case study in conservation biology to investigate the possible role played by introgressive hybridization in the adaptation to recent land use and vegetation cover changes associated with rural abandonment in an insular yet heavily anthropized context.

Morphologic and Histologic Observation of Red-Legged Partridge's (Alectoris Chukar) Liver

This study was aimed to investigate the liver of red-legged partridge in regards of morphologic and histologic features. In this study, the liver tissues of ten red-legged partridges were removed and fixed into 10% formaldehyde solution for 72 hours. After fixation, the liver tissues were dehydrated through a graded alcohol series to xylene and embedded in paraffin blocks. Obtained sections 5-7 µm thickness of sections from paraffin blocks were stained with Crossman Modified Triple staining and examined for histologic structures. In morphologic examining, the liver of red-legged partridges consisted of two lobs. In addition, histologic analysis showed that liver tissue was covered with a thick connective tissue and this connective tissue made up of many smaller units of liver cells called lobules. Hepatocytes were seen radially round and located around central vena, which consists of remark cords. The squamous cells and Kupffer cells were observed in the sinusoidal lining. In conclusion; the results from this study indicated that there were some structural differences from other bird species, but not functionally.

Lead in terrestrial game birds from Spain.

We analysed exposure to Pb and its relationship with lead-based ammunition in seven species of terrestrial game birds-common woodpigeon (Columba palumbus), rock dove (Columba livia), stock dove (Columba oenas), European turtle-dove (Streptopelia turtur), red-legged partridge (Alectoris rufa), Barbary partridge (Alectoris barbara) and common quail (Coturnix coturnix)-from rural and urban areas in different parts of Spain (Valencia, Castilla-La Mancha, Castilla y León, Madrid, Islas Canarias and Navarra). A total of 530 liver samples were analysed, and the presence of Pb pellets was studied in the crop, gizzard and intestine; the state and appearance of these organs were also analysed. The number of specimens suspected to have ingested Pb shot was 28 (5.6%), and the geometric mean concentration of hepatic Pb was 0.054 μg g-1 (wet weight, ww). A low percentage of samples (4.8%) were above the abnormal exposure threshold (0.65 μg g-1 ww), and, in these specimens, renal Pb concentrations were determined. Common woodpigeons and rock doves from Madrid were found to have high concentrations of Pb in their livers, and, so, both species can be considered to be good bioindicators of Pb contamination in rural (common woodpigeons) and urban (rock doves) environments. Partridges bred for hunting may be more prone to ingesting pellets from the environment, a fact that should be taken into account in management decisions.

Correction factors for estimating food consumption by red fox Vulpes vulpes from scats

Assessments of terrestrial carnivore diet are often required for ecological management and conservation purposes. Analysis and identification of food remains in scats is an important method often employed for these objectives. Proportions of consumed biomass estimated from scat samples may provide an accurate approximation of the actual diet when correction factors (CFs) are used. The red fox Vulpes vulpes is a widespread generalist carnivore that includes a wide range of foods in its diet, including livestock and plays a potential role as wildlife predator. We performed controlled feeding trials with red foxes to derive CFs for eight food categories, including both from animal and vegetal origin. CFs were calculated on a daily basis as the ratio between eaten fresh mass and total dry mass of the resulting scats. Red foxes consumed daily 615 ± 19 g (mean ± SE) of food and produced 6.4 ± 0.3 scats weighing 31 ± 1.2 g. The CFs varied significantly among food types, with the lowest values for partridges Alectoris rufa (CF = 12.5 ± 0.7) and the highest for red deer Cervus elaphus (CF = 47.3 ± 7.3), increasing linearly and significantly in relation to the individual body mass for the animal food types. CF precision was low for deer, fruit and hare Lepus granatensis, but more importantly, the CFs differed considerably among food types. We recommend considering intra-food variability when using CFs for estimating proportions of ingested biomass in studies of generalist carnivores such as red fox as a better support for management and conservation decisions.

Using long-term datasets to assess the impacts of dietary exposure to neonicotinoids on farmland bird populations in England.

Over the last 20 years, a new group of systemic insecticides–the neonicotinoids—has gained prominence in arable systems, and their application globally has risen year on year. Previous modelling studies using long-term data have suggested that neonicotinoid application has had a detrimental impact on bird populations, but these studies were either limited to a single species or neglected to analyse specific exposure pathways in conjunction with observed population trends. Using bird abundance data, neonicotinoid usage records and cropping data for England at a 5x5 km resolution, generalised linear mixed models were used to test for spatio-temporal associations between neonicotinoid use and changes in the populations of 22 farmland bird species between 1994 and 2014, and to determine whether any associations were explained by dietary preferences. We assigned farmland bird species to three categories of dietary exposure to neonicotinoids based on literature data for species diets and neonicotinoid residues present in dietary items. Significant estimates of neonicotinoid-related population change were obtained for 13 of the 22 species (9 positive effects, 4 negative effects). Model estimates for individual species were not collectively explained by dietary risk categories, so dietary exposure to neonicotinoids via ingestion of treated seeds and seedlings could not be confirmed as a causal factor in farmland bird declines. Although it is not possible to infer any generic effect of dietary exposure to neonicotinoids on farmland bird populations, our analysis identifies three species with significant negative estimates that may warrant further research (house sparrow Passer domesticus, skylark Alauda arvensis and red-legged partridge Alectoris rufa). We conclude that there was either no consistent effect of dietary exposure to neonicotinoids on farmland bird populations in England, or that any over-arching effect was not detectable using our study design. The potential for indirect effects of insecticide use on bird populations via reduced food availability was not considered here and should be a focus for future research.

Restoring apex predators can reduce mesopredator abundances

The role that apex predators play in ecosystem functioning, disease regulation and biodiversity maintenance is increasingly debated. However, the positive impacts of their presence in terrestrial ecosystems, particularly in human-dominated landscapes, remain controversial. Limited experimental insights regarding the consequences of apex predator recoveries may be behind such controversy and may also impact on the social acceptability towards the recovery of these species. Using a quasi-experimental design and state-of-the-art density estimates, we show that mesopredator abundances were reduced after the restoration of an apex predator, with evidence of resonating positive impacts on lower trophic levels. Iberian lynx Lynx pardinus reintroduction was followed by the reduction of the abundance of mesocarnivores (red foxes Vulpes vulpes and Egyptian mongooses Herpestes ichneumon by ca. 80%) and the recovery of small game of high socio-economic value (European rabbits Oryctolagus cuniculus and red-legged partridges Alectoris rufa). The observed mesopredator reduction resulted in an estimated 55.6% less rabbit consumption for the entire carnivore guild. Our findings have important implications for the social acceptability of Iberian lynx reintroductions, which crucially depend on the perception of private land owners and managers. Under certain circumstances, restoring apex predators may provide a sustainable and ethically acceptable way to reduce mesopredator abundances.

Carotenoid-based coloration predicts both longevity and lifetime fecundity in male birds, but testosterone disrupts signal reliability.

Sexual selection promotes the evolution of conspicuous animal ornaments. To evolve as signals, these traits must reliably express the “quality” of the bearer, an indicator of individual fitness. Direct estimates of individual fitness may include the contribution of longevity and fecundity. However, evidence of a correlation between the level of signal expression and these two fitness components are scarce, at least among vertebrates. Relative fitness is difficult to assess in the wild as age at death and extra-pair paternity rates are often unknown. Here, in captive male red-legged partridges, we show that carotenoid-based ornament expression, i.e., redness of the bill and eye rings, at the beginning of reproductive life predicts both longevity (1–7 years) and lifetime breeding output (offspring number and hatching success). The recently proposed link between the individual capacity to produce red (keto) carotenoid pigments and the efficiency of cell respiration could, ultimately, explain the correlation with lifespan and, indirectly, fecundity. Nonetheless, in males of avian species, carotenoid-based coloration in bare parts is also partially controlled by testosterone. We also manipulated androgen levels throughout life by treating males with testosterone or antiandrogen compounds. Treatments caused correlations between signal levels and both fitness components to disappear, thus making the signals unreliable. This suggests that the evolution of carotenoid-based sexual signals requires a tightly-controlled steroid metabolism.

Anti-predator behavior in pure and hybrid red-legged partridges

The purpose of this study was to examine the anti-predator behavior in pure red-legged partridges (Alectoris rufa) and hybrids with chukar partridges (A. rufa × (A. rufa × A. chukar)). A total of 117 birds (66 pure and 51 hybrid partridges; 74 females and 43 males) maintained in outdoor cages were used at a range of 52 to 55 wk of age. Four active fear tests were performed (novel object test, fear of humans test, emergence test, and restraint test) in order to analyze differences between pure and hybrid populations regarding the escape response type (novel object test and fear of humans test), the withdrawal distance (fear of humans test), and the reaction time (emergence test and restraint test). These variables could determine the ability of pure partridges captive to survive when released into the wild for hunting. There were no significant differences in anti-predator behavior in pure and hybrid birds, only one trend was observed in withdrawal distance (P=0.09), being greater in pure than in hybrids partridges. There was a significant difference between sexes in the response type in the novel object test (P=0.05); whereby the females came closer to the object than the males. The results of the present study indicate that active anti-predatory behavior was not associated with the genotype. However, since captivity could reduce the active anti-predator behavior, the results could be different if the wild birds that were tested had been sourced from their natural environment. Both sexes had similar active anti-predator behavior, although the males showing less approach behavior to a novel object that could be related to the sex-dimorphic behavior in natural social structure.

Associations between gamebird releases and generalist predators

Abstract The release of more than 40 million captive‐bred pheasants and red‐legged partridges in Britain annually represents a significant addition to the potential food resource base for predators and scavengers. If this extra food availability subsidizes predator populations, gamebird releases could increase predation pressure on other wild birds, affecting their populations. Using three extensive datasets, we examined the spatial relationships between reared and free‐roaming gamebirds (pheasant Phasianus colchicus and red‐legged partridge Alectoris rufa), and explored spatial and temporal associations between these gamebirds and five species of avian predator (buzzard Buteo buteo, jay Garrulus glandarius, raven Corvus corax, magpie Pica pica and hooded Corvus cornix and carrion Corvus corone crows combined) in lowland rural Britain. Patterns of spatial variation in the abundance of free‐roaming gamebirds across Britain appear to be largely determined by gamebird releases, over and above any effects of land use or habitat. Predominantly positive associations between gamebird abundance (both reared and free‐roaming) and the abundance and inter‐annual population growth rates of predators tested suggest that large‐scale variation in avian predator populations may be positively affected by gamebird releases. Synthesis and applications. The positive associations between large‐scale gamebird release and predator populations shown here may have implications for prey populations if the releases cause increased predation pressure. If this occurs, game management could have an indirect negative impact on some prey species partially counteracting previously reported positive or benign effects of game management on wider biodiversity. Overall impacts of gamebird releases are likely to be determined by complex interactions between multiple factors, including induced predation pressure, better understanding of which would be possible with compulsory recording of releases and numbers of predators killed. Restriction of releases warrants further investigation and consideration as a potential conservation tool for wild bird populations.

Influence of flavivirus co-circulation in serological diagnostics and surveillance: A model of study using West Nile, Usutu and Bagaza viruses.

This study aims at assessing the serological cross-reactions existing between three mosquito-borne flaviviruses with avian reservoirs co-circulating in Europe: West Nile (WNV), Usutu (USUV) and Bagaza (BAGV). The study is useful for a better interpretation of serological results in diagnostics and surveillance. Serum samples obtained from a natural host, the red-legged partridge (Alectoris rufa), experimentally infected with WNV, USUV or BAGV were analysed using two commercially available WNV competition ELISAs suitable for serological surveillance, and by the confirmatory virus neutralization test (VNT). The ELISAs examined showed different levels of specificity for WNV, as judged by cross-reaction observed with the other flaviviruses. By VNT, virus-specific antibodies were confirmed in 80%, 50% or 0% of sera from WNV-, BAGV-, or USUV-inoculated birds, respectively. The results indicate how the co-circulation of cross-reacting flaviviruses may affect the outcomes of WNV serological surveillance when applying currently available serological tools. On the one hand, the choice of the ELISA test for antibody screening should consider the differences found in specificity, since one test is more specific for WNV while the other one is more suitable for detection of a broader range of flavivirus antibodies. On the other hand, besides corroborating that cross-neutralization occurs between flaviviruses from different serocomplexes (WNV/USUV and BAGV), this study points out that cross-neutralization between WNV and USUV is not symmetric, and reveals the difficulty to identify USUV infections serologically. This finding indicates that actual USUV infections might be underestimated in the current diagnostic schemes.

Nutritional value of meat lipid fraction from red-legged partridge (Alectoris rufa) obtained from wild and farmed specimens1.

The red-legged partridge (Alectoris rufa) is a feathered game species of great socioeconomic importance in its native range and also in the UK. The aim of this study was to present a detailed comparison of meat's lipid fraction obtained from wild and farm-raised specimens and simultaneously compare the breast and leg meat portions. Meat from wild specimens had a significant (P < 0.05) lower proportion of saturated fatty acid (less 5.1%) and presented better P/S and n-6/n-3 ratios, and atherogenicity index than farm-raised counterparts. The wild specimens presented significant (P < 0.001) higher contents of total vitamin E (8.8 vs. 2.2μg/g of fresh meat), is for that reason less prone to lipid peroxidation than farm-raised specimens. Meat portions differed significantly (P < 0.05) on total lipid and total cholesterol contents and in all partial sums of fatty acids. The breast was leaner (0.86 vs. 1.47g/100g of meat), with lower total cholesterol (37.5 vs. 54.7mg/100g of meat), lower saturated fatty acid, monounsaturated fatty acid, polyunsaturated fatty acid, and n-6 polyunsaturated fatty acid (less 0.27, 0.28, 0.10, and 0.11g/100g of fresh meat, correspondingly). Regarding the fatty acid ratios and lipid quality indexes, breast meat presents better n-6/n-3 ratio and atherogenicity and thrombogenicity indexes.

Comparison of Several Turning Frequencies During the Storage Period of Red-Legged Partridge (Alectoris rufa) Hatching Eggs

ABSTRACT The effects of three egg-turning regimes during the pre-incubation storage period on egg weight loss, hatchability, embryonic mortality, chick weight at hatching and incubation length of red-legged partridge (Alectoris rufa) compared with unturned eggs were investigated. Two hundred eggs were allocated to four 50-egg batches stored at 15oC and 80% RH that being turned one, four and 24 times a day or remaining unturned, respectively. Eggs were incubated at 37.8oC and 55% RH during the first 21 days and at 37.5oC and 75% RH until hatching. Fertility was 70.5% and a good hatchability performance was obtained, characterised by 81.6% hatchability of fertile eggs, and weight losses of 0.78% during storage and of 10.04% during the first 21 days of incubation, 13.6 ±0.1 g hatchling weight, and incubation length of 23.45 ± 0.07 days (mean ± SEM). Hatchability, embryonic mortality developmental stage, egg weight loss during storage and incubation, hatchling weight and length of the incubation period were not affected by the turning frequency or the absence of turning during storage. Higher hatching synchrony was observed for eggs turned four times a day compared with unturned eggs and eggs turned once a day. In conclusion, turning red-legged partridge eggs during medium-term storage periods does not improve egg viability compared with unturned eggs.

A black Page in the French partridge's history: the melanistic variety of Red-legged Partridge Alectoris rufa

Summary. The melanistic variety of Red-legged Partridge Alectoris rufa was described from a small population in western France around the 1850s. In this region, the Red-legged Partridge population as a whole was hunted, but melanistic individuals were targeted for both private and museum bird collections, and by 1865 the variety was extinct in western France. An extensive search for extant specimens documented 13 melanistic birds in six museums, and their details are presented here. Remarkably, some of these specimens were collected in areas elsewhere in France or even in other countries. After 1915, the allele for melanism appears to have been lost within the Red-legged Partridge population as a whole, and we discuss possible reasons for this. ‘…, nous sommes persuade que cette perdrix qui tend a demeurer en Anjou [now Maine-et-Loire] se multipliera dans quelques annees, au grand contentement des amateurs de la chasse et de l'ornithologie.’ […, we are persuaded that this partridge which tends to remain i...

Body proportions for the facilitation of walking, running and flying: the case of partridges

BackgroundPredation is one of the most important natural selection forces. Prey species can optimize feeding behavior and escape from predators based on mobility conditioned by body proportions. With age, mobility capacity increases and individuals are more efficient in finding resources and safety (e.g., food and refuge). Birds’ mobility is driven by the dimensions, of the head and torso, as well as the extremities and flight feathers. To assess the relationship between body traits and to understand how body proportions are organized in wild Red-legged partridges (Alectoris rufa), we used biometric data from nearly 14,000 individuals, obtained during a long-term study (1988–2011) on a wild population.ResultsWe used GLMs and regressions to model the relationship between body mass and the size of body parts. We found that wing length was the morphological part best explained by other body trait measures. Wing length models were better predictors in juveniles than in adults and in females than in males. Wing length and feather length, mass and total length are the most strongly related parts; mass and wing length, total length and feather length are moderately related. The association between mass and wing length is intermediated by feather length and total length.ConclusionsSocial inclusion, feeding and predator evasion may be affected by body structure intermediated by mobility and health. Our results suggest that proportions of the body, extremities and flight feathers drive mobility which is intimately associated with ecology, biological efficiency, health and physical optimization. Our findings showed that wing size was strongly allied to other body part measurements, enhancing the importance of body structure conformation for flight. Our study highlights the scaled relationship of body structure among age-sex classes and its relevance to social cohesion, flock movement and the balance between predation and starvation.

Red-legged partridges perceive the scent of predators and alarm scents of an avian heterospecific

A growing body of evidence suggests that birds can use olfactory cues to detect the presence of predators. We predicted that the ability to gather information about predator presence through chemical cues might be particularly important for ground-living and foraging bird species, since their main predators, namely mammals and reptiles, use chemical communication. In this context, we experimentally examined the role of olfaction in the nondomesticated, ground-living red-legged partridge, Alectoris rufa, in a natural and context-dependent situation. We tested how individuals responded to three different olfactory stimuli, which were presented in sand bath tubs: (1) a mammalian predator (ferret faeces), (2) an avian alarm scent (alarm secretion of the European hoopoe) and (3) a control (orange oil). As dependent variables we recorded side preferences and dustbathing activity. Red-legged partridges avoided the predator scent to the same extent as the alarm scent of the hoopoe, whereas orange oil scent did not affect side choice or dustbathing behaviour of individuals. Our results indicate that red-legged partridges avoid the scent of a predator as well as the alarm scent of an avian heterospecific. In regard to this, we provide the first indication that, in risk assessment, chemical cues, similar to avian alarm calls, may possibly act as a source of information between avian species.

Brood size is reduced by half in birds feeding on flutriafol-treated seeds below the recommended application rate

Despite the efforts of the European Commission to implement measures that offset the detrimental effects of agricultural intensification, farmland bird populations continue to decline. Pesticide use has been pointed out as a major cause of decline, with growing concern about those agro-chemicals that act as endocrine disruptors. We report here on the effects of flutriafol, a ubiquitous systemic fungicide used for cereal seed treatment, on the physiology and reproduction of a declining gamebird. Captive red-legged partridges (Alectoris rufa; n = 11–13 pairs per treatment) were fed wheat treated with 0%, 20% or 100% of the flutriafol application rate during 25 days in late winter. We studied treatment effects on the reproductive performance, carotenoid-based coloration and cellular immune responsiveness of adult partridges, and their relationship with changes in oxidative stress biomarkers and plasma biochemistry. We also studied the effect of parental exposure on egg antioxidant content and on the survival, growth and cellular immune response of offspring. Exposed partridges experienced physiological effects (reduced levels of cholesterol and triglycerides), phenotypical effects (a reduction in the carotenoid-based pigmentation of their eye rings), and most importantly, severe adverse effects on reproduction: a reduced clutch size and fertile egg ratio, and an overall offspring production reduced by more than 50%. No effects on body condition or cellular immune response of either exposed adult or their surviving offspring were observed. These results, together with previous data on field exposure in wild partridges, demonstrate that seed treatment with flutriafol represents a risk for granivorous birds; they also highlight a need to improve the current regulation system used for foreseeing and preventing negative impacts of Plant Protection Products on wildlife.

Game meat consumption by hunters and their relatives: a probabilistic approach

ABSTRACTThis study aimed to estimate the consumption of meat and products derived from hunting by the consumer population and, specifically, by hunters and their relatives. For this purpose, a survey was conducted on the frequency of consuming meat from the four most representative game species in Spain, two of big game, wild boar (Sus scrofa) and red deer (Cervus elaphus), and two of small game, rabbit (Oryctolagus cuniculus) and red partridge (Alectoris rufa), as well as of processed meat products (salami-type sausage) made from those big game species. The survey was carried out on 337 habitual consumers of these types of products (hunters and their relatives). The total mean game meat consumption, per capita in this population group, is 6.87 kg/person/year of meat and 8.57 kg/person/year if the processed meat products are also considered. Consumption of rabbit, red partridge, red deer and wild boar, individually, was 1.85, 0.82, 2.28 and 1.92 kg/person/year, respectively. It was observed that hunters generally registered a larger intake of game meat, this being statistically significant in the case of rabbit meat consumption. Using probabilistic methods, the meat consumption frequency distributions for each hunting species studied were estimated, as well as the products made from big game species and the total consumption both of meat by itself and that including the products made from it. The consumption frequency distributions were adjusted to exponential ones, verified by the test suitable for it according to Akaike Information Criterion (AIC), Bayesian Information Criterion (BIC), the Chi-squared and Kolmogorov–Smirnov statistics. In addition, the consumption percentiles of the different distributions were obtained. The latter could be a good tool when making nutrition or contaminant studies since they permit the assessment of exposure to the compound in question.