Red Cedar Research Articles

Optimized method for differential gene expression analysis in non-model species: Case of Cedrela odorata L.

The following protocol introduces a targeted methodological approach of differential gene expression analysis, which is particularly beneficial in the context of non-model species. While we acknowledge that biological complexity often involves the interplay of multiple genes in any given biological response our method provides a strategy to streamline this complexity, enabling researchers to focus on a more manageable subset of genes of interest. In this context, red cedar transcriptome (Cedrela odorata L.) and known or hypothetical genes related to the response to herbivory were used as reference. The protocol key points are:•Implementation of a transcriptome thinning process to eliminate redundant and non-coding sequences, optimizing the analysis and reducing processing time.•Use of a custom gene database to identify and retain coding sequences with high precision.•Focus on specific genes of interest, allowing a more targeted analysis for specific experimental conditions.This approach holds particular value for pilot studies, research with limited resources, or when rapid identification and validation of candidate genes are needed in species without a reference genome.

Effective stakeholder engagement in environmental problem-solving though group model building: An Oklahoma case study

Stakeholder engagement is a vital, yet under-accessed and under-studied, resource for tackling wicked environmental problems. This paper examines mental models – cognitive representations of “real” systems – refined through group model building (GMB) with stakeholders concerned with environmental issues in the US state of Oklahoma. During GMB, a diverse group of high-level decision makers met with a variety of physical and social scientists to collaborate on mental models concerning three environmental focus areas in Oklahoma: encroachment of eastern red cedar, grid and infrastructure resilience, and marginal water use and re-use. We ask: how do individuals from diverse stakeholder groups describe causes and consequences of key environmental problems in Oklahoma? Results from this analysis advance efforts toward developing socially sustainable solutions for environmental problems in Oklahoma and beyond.

Tree Trunk Detection of Eastern Red Cedar in Rangeland Environment with Deep Learning Technique

Uncontrolled spread of eastern red cedar invades the United States Great Plains prairie ecosystems and lowers biodiversity across native grasslands. The eastern red cedar (ERC) infestations cause significant challenges for ranchers and landowners, including the high costs of removing mature red cedars, reduced livestock forage feed, and reduced revenue from hunting leases. Therefore, a fleet of autonomous ground vehicles (AGV) is proposed to address the ERC infestation. However, detecting the target tree or trunk in a rangeland environment is critical in automating an ERC cutting operation. A tree trunk detection method was developed in this study for ERC trees trained in natural rangeland environments using a deep learning-based YOLOv5 model. An action camera acquired RGB images in a natural rangeland environment. A transfer learning method was adopted, and the YOLOv5 was trained to detect the varying size of the ERC tree trunk. A trained model precision, recall, and average precision were 87.8%, 84.3%, and 88.9%. The model accurately predicted the varying tree trunk sizes and differentiated between trunk and branches. This study demonstrated the potential for using pretrained deep learning models for tree trunk detection with RGB images. The developed machine vision system could be effectively integrated with a fleet of AGVs for ERC cutting. The proposed ERC tree trunk detection models would serve as a fundamental element for the AGV fleet, which would assist in effective rangeland management to maintain the ecological balance of grassland systems.

From Gain to Bane—and Back Again: What’s Next for Eastern Red Cedar?

CSA NewsVolume 68, Issue 6 p. 8-12 FEATURES From Gain to Bane—and Back Again: What’s Next for Eastern Red Cedar? Kristen Coyne, Kristen CoyneSearch for more papers by this author Kristen Coyne, Kristen CoyneSearch for more papers by this author First published: 29 May 2023 https://doi.org/10.1002/csan.21051Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Volume68, Issue6June 2023Pages 8-12 RelatedInformation

Eastern red cedar effects on carbon sequestration and soil quality in the Great Plains

AbstractEastern red cedar (ERC, Juniperus virginiana L.) is a common tree species in agroforestry plantings and has great potential for bioenergy production due to physical and chemical characteristics of its biomass. The objective of this study was to assess the effects of ERC plantings on carbon (C) sequestration and selected soil quality parameters in existing plantings across the northern U.S. Great Plains. Nine locations were selected in five states with mean annual precipitation (MAP) from 446 to 999 mm and mean annual temperature (MAT) from 4.4 to 10.0°C. Infiltration was measured using the twin ring technique at nine sites in each tree planting and adjacent field (crop, pasture, or hay) at each location. Following infiltration measurements, a 4.8 cm‐diameter soil core to 30 cm depth was collected from inside one infiltration ring of each pair for bulk density, pH, aggregate stability, soil organic carbon (SOC), and total nitrogen (TN) analyses. Penetration resistance in 2.5 cm increments to 30 cm depth was measured in the other infiltration ring. SOC stocks under ERC were significantly higher than in the adjacent field soil at five of the locations and, for all locations, averaged 16.8% greater than in the adjacent field. The estimated SOC accumulation rate for the eight locations with uniform tree stand age averaged 0.30 Mg C ha−1 year−1. Most locations had higher TN and C/N and lower bulk density beneath ERC. Fewer differences for infiltration and penetration resistance and smaller and less consistent differences for pH and aggregate stability were observed. Tree aboveground C stocks were estimated to increase an average of 2.05 Mg C ha−1 year−1. Estimated tree aboveground biomass C and SOC stocks under ERC and in adjacent fields were strongly correlated with MAP. Potential ERC planting for C sequestration or bioenergy feedstock production is not likely to significantly degrade soil quality and may improve soil physical or chemical quality for locations in the US Great Plains.

Investigating the effects of wind loading on three dimensional tree models using numerical simulation with implications for urban design

In this study, the effects of wind on an Eastern Red Cedar were investigated using numerical simulations. Two different tree models were proposed, each with varying bole lengths and canopy diameters. A total of 18 cases were examined, including different canopy diameters, bole lengths, and wind velocities. Using computational fluid dynamics (CFD) methods, the drag force, deformation, and stress of the tree models were calculated under different wind velocities and geometric parameters. A one-way fluid–structure interaction (FSI) method was used to solve the deformation of the tree. Additionally, velocity and pressure distribution around the tree were obtained. The results indicate that wind velocity and geometric parameters of the tree have a significant impact on deformation, drag force, and stress. As wind velocity increases from 15 to 25 m/s, the force on the tree increases substantially. The results also show that the diameter of the canopy has a bigger effect on stress and strain than the bole length. This study provides insights into tree behavior under wind loading for urban planning and design, informing optimal tree selection and placement for windbreak effectiveness and comfortable environments.

Near-field measurements for wood anisotropy using cavity-backed slot antennas

Wood is becoming a very important structural material for buildings because it is a renewable resource and has a very small carbon footprint. This paper reports an experimental study on the effect of annual rings on wood anisotropy measurements using near-field microwave scanning for Pine, Hemlock, Red Cedar, and Hardwood laminated wood beams. Transmission measurements show significant anisotropy (>10 dB) for Pine wood compared to Hemlock wood and Red Cedar wood for linear polarization. The effect of annual rings in guiding the transmitted signal through wood fibers is observed through transmission measurements. Microwave guidance in transmission disappears with increasing the moisture content (17%–138%) of the Pine wood sample. The study provides a novel, accurate, fast, low-cost structural-wood testing method based on cavity-backed slot antennas for transmission and reflection measurements.

The community of soil fungi associated with the western red cedar (Thuja plicata Donn ex D. Don, 1824)

ABSTRACT The western red cedar (Thuja plicata Donn ex D. Don), an important forest-forming species in the Western part of the North American continent, is an alien species naturalised in Europe. It is popular and highly valued in horticulture. While considering the progressing climate change, it may also be a potential alternative to native species in European forests. The community of soil fungi associated with the western red cedar in forested areas of Europe has not been fully determined. Thus, this study is aimed to identify the community of soil fungi associated with the western red cedar. The experimental plots are located in the Kościan Forest District (51°98’87″ N; 16°23’54″ E). All soil samples were taken from the topsoil layer at a depth of 25 cm with a trowel, three from the centre of natural regeneration (1G, 2G, 5G) and three from the centre stand under the canopy of old-growth western red cedar (3G, 4G, 6G). Fungi were identified directly from the soil based on the ITS1 rDNA region. The derived product was sequenced using Illumina’s sequencing by synthesis (SBS) technology. Sequences were referred to the National Center for Biotechnology Information (NCBI) database applying the BLAST algorithm. The fungal counts were defined based on the number of operational taxonomic units (OTU) in the sample. The OTU number was 835 206, with fungal isolates accounting for 683 095 (81.79%). A total of 8 591 taxa belonging to the Kingdom Fungi were identified. The species with the greatest shares in the community included Mortierella spp. (10.5%), Russula spp. (5.6%), Hydnum spp. (3.44%), Solicoccozyma spp. (3.1%) and Penicillium spp. (2.2%). Results showed that saprotrophs and mycorrhizal fungi predominated in the community. The dominance of ectomycorrhizal fungi over arbuscular ones, quite impressive natural regeneration was shown in T. plicata stands in Kościan. Subsequent research should take into account tree stands in Poland in which natural regeneration does not occur or occurs sporadically.

The rapid-tome, a 3D-printed microtome, and an updated hand-sectioning method for high-quality plant sectioning

BackgroundMicroscopic analysis of plant anatomy is a common procedure in biology to study structure and function that requires high-quality sections for accurate measurements. Hand sectioning of specimens is typically limited to moderately soft tissue while harder samples prohibit sectioning by hand and/or result in inconsistent thicknesses.ResultsHere we present both a clearly described hand-sectioning method and a novel microtome design that together provide the means to section a variety of plant sample types. The described hand-sectioning method for herbaceous stems works well for softer subjects but is less suitable for samples with secondary growth (e.g., wood production). Instead, the “Rapid-Tome” is a novel tool for sectioning both soft and tougher high-aspect-ratio samples, such as stems and roots, with excellent sample control. The Rapid-Tome can be 3D-printed in approximately 18 h on a mid-quality printer common at university maker spaces. After printing and trimming, Rapid-Tome assembly takes a few minutes with five metal parts common at hardware stores. Users sectioned a variety of plant samples including the hollow internodes of switchgrass (Panicum virgatum), fibrous switchgrass roots containing aerenchyma, and woody branches of eastern red cedar (Juniperus virginiana) and American sycamore (Platanus occidentalis). A comparative analyses with Rapid-Tome-produced sections readily revealed a significant difference in seasonal growth of sycamore xylem vessel area in spring (49%) vs. summer (23%). Additionally, high school students with no prior experience produced sections with the Rapid-Tome adequate for comparative analyses of various plant samples in less than an hour.ConclusionsThe described hand-sectioning method is suitable for softer tissues, including hollow-stemmed grasses and similar samples. In addition, the Rapid-Tome provides capacity to safely produce high-quality sections of tougher plant materials at a fraction of the cost of traditional microtomes combined with excellent sample control. The Rapid-Tome features rapid sectioning, sample advancement, blade changes, and sample changes; it is highly portable and can be used easily with minimal training making production of thin sections accessible for classroom and outreach use, in addition to research.

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Analysis of the thermal exposure and ignition propensity of a lignocellulosic building material subjected to a controlled deposition of glowing firebrands

A series of experiments was carried out in a bench-scale wind tunnel where firebrand piles of controlled coverage density and geometry were deposited onto a flammable and non-flammable substrate, Western Red Cedar (WRC) and Kaowool PM, respectively. Back substrate temperature, combustion heat release rate, modified combustion efficiency, and gaseous flame ignition and extinction statistics were collected. For a firebrand pile covering 5 × 10 cm2 area of the WRC substrate at 0.16 g cm−2 density, the probability of the substrate ignition increased from 11 to 55% with the air flow velocity increasing from 0.9 to 2.4 m s−1. The probability decreased upon further increase in the air flow. At 2.4 m s−1, the probability of the substrate ignition decreased to 11% when the firebrand coverage density was reduced to 0.06 g cm−2. Qualitatively similar trends were observed for the maximum Kaowool PM back surface temperature and peak heat release rate. The WRC's maximum contribution to the combustion heat release rate was found to be < 12% of the maximum contribution of the firebrand pile. Almost all WRC surface ignitions were observed to occur at the air flow facing edge of the firebrand pile. When the length of the air flow facing edge was increased by a factor of two, the probability of ignition nearly doubled.

Factors affecting the adoption of patch-burn grazing in the southern Great Plains in the US

Patch-burn grazing, a combination of fire and grazing, has been identified as a novel approach to maintaining biodiversity in the Great Plains of the United States. Many ranchers, however, are not aware of the practice and very few of them had adopted it on their land. Utilizing the data obtained from a survey of landowners residing in the southern Great Plains of the United State conducted in 2021, we analyzed the factors affecting the awareness and adoption of patch-burn grazing. Since an adoption behavior study might suffer from selection bias if the level of awareness is not appropriately accounted for, we used a bivariate probit model for data analysis. The study results indicated that repeated wildfires, nature conservancy, and university/county extension positively affected the probability of patch-burn grazing awareness while the effect of age and livestock membership had a negative impact. The research further revealed that larger landholding size, management objectives to control blackberry, repeated wildfires, and university/county extension positively influenced the likelihood of patch-burn grazing adoption. In contrast, the management objective to control red cedar, along with respondent age, and higher-income negatively influenced the patch-burn grazing adoption. Since many landowners are not convinced to adopt patch-burn grazing, study results suggest further extension needs to educate traditional and non-traditional ranchers on the ecological and production benefits of patch-burn grazing.

Evaluation of the effects of wood-sourced biochar as a feedlot pen surface amendment on manure nutrient capture.

Feedstuffs utilized in U.S. feedlot finishing rations incorporate high concentrations of N and P, with less than 15% of fed N and P retained by the animal. The remaining N and P are excreted in the manure, where the opportunity for manure N loss via ammonia (NH3) volatilization from the feedlot pen surface is a risk to the environment and lowers the value of manure as a fertilizer. Two nutrient mass balance experiments were conducted during the winter and summer seasons to evaluate the effects of spreading unprocessed Eastern red cedar biochar onto the feedlot pen surface on manure nutrient capture and cattle performance. A 186-d feedlot finishing experiment was conducted from December to June (WINTER) and a subsequent 153-d finishing experiment was conducted from June to November (SUMMER). The WINTER experiment evaluated three treatments (5 pens per treatment; 10 steers per pen), including biochar spread on pen surface during the feeding period (1.40 kg biochar/m2; 17.6 m2/steer soil surface of the pen), hydrated lime spread on pen surface at end of feeding period (1.75 kg/m2) and control (no treatment applied). The SUMMER experiment evaluated biochar treatment (1.40 kg biochar/m2; 5 pens per treatment; 8 steers per pen; and 22 m2/steer soil surface of the pen) against control. There were no differences in N and P intake, retention, or excretion (P ≥ 0.38) between WINTER treatments. Steer performance (P ≥ 0.10) and carcass characteristics (P ≥ 0.50) were not impacted by pen treatment in WINTER. Nitrogen and P intake and excretion (P ≥ 0.35) were not different between treatments in SUMMER and retention of N and P was significantly greater for the biochar treatment (P ≤0.04) due to greater ADG (P = 0.05). There was no difference in DMI (P = 0.48) in SUMMER, steers on biochar pen treatment had heavier HCW (P = 0.05) and greater ADG, resulting in a tendency for greater feed efficiency (P = 0.08). In both experiments, biochar addition to the pen surface tended (P = 0.07) to increase manure N as a percent of manure DM, but this increase in N concentration did not impact kg of N removed from the feedlot pens (P ≥ 0.15) or N losses (P ≥ 0.68). The addition of red cedar biochar to the feedlot pen surface did not increase manure nutrient capture of N or P and did not reduce N losses associated with soil-based feedlot pens.

An analysis of conifer experiments in Britain to identify productive alternatives to Sitka spruce

Abstract As the climate changes there is a need to reduce the reliance on Sitka spruce (Picea sitchensis (Bong) Carr.) in upland British forestry and increase species diversity to lower the risk of damage or loss of production due to biotic or abiotic events. An analysis of relevant species trials was carried out to assess the productivity of potential alternative conifer species on upland site types in Britain. Data from 87 forest experiments planted between 1929 and 1995 were analysed to compare long-term performance of 52 species with that of Sitka spruce under the same conditions and site type. Sites were broadly categorized using soil and climatic factors, which were used as primary factors in models predicting General Yield Class (GYC, the maximum mean annual increment in m3 ha−1 year−1) of the potential alternative species and of Sitka spruce. No species had significantly higher GYC estimates than Sitka spruce, but grand fir (Abies grandis (Douglas ex D. Don) Lindl.), noble fir (Abies procera (Rehder)), western hemlock (Tsuga heterophylla (Raf.) Sarg.), Lutz’s spruce (Picea x lutzii (Little)), maritime pine (Pinus pinaster (Aiton)), Lawson cypress (Chamaecyparis lawsoniana (A. Murray bis) Parl.), Scots pine (Pinus sylvestris (L.)), Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) and western red cedar (Thuja plicata (Donn ex D.Don)) all had GYC estimates which were not significantly lower than that of Sitka spruce under certain soil and climate conditions. A further 26 species, particularly Abies species, had GYC within 3 m3 ha−1 year−1 of Sitka spruce but were present on too few sites for inclusion in statistical models. The results of the analysis provide objective evidence for the planting of a wider range of species where Sitka spruce may currently be first choice. In the absence of any major pest or disease affecting Sitka spruce, it is still a good choice for many upland sites, however, a wider range of options with equivalent productivity exist, allowing forest managers to diversify and reduce the risk of damage or loss of production due to biotic or abiotic events.

Protecting playgrounds: local-scale reduction of airborne particulate matter concentrations through particulate deposition on roadside ‘tredges’ (green infrastructure)

Exposure to traffic-related particulate air pollution has been linked with excess risks for a range of cardiovascular, respiratory and neurological health outcomes; risks likely to be exacerbated in young children attending schools adjacent to highly-trafficked roads. One immediate way of reducing airborne PM concentrations at the local (i.e., near-road community) scale is installation of roadside vegetation as a means of passive pollution abatement. Roadside vegetation can decrease airborne PM concentrations, through PM deposition on leaves, but can also increase them, by impeding airflow and PM dispersion. Critical to optimizing PM removal is selection of species with high particle deposition velocity (Vd) values, currently under-parameterised in most modelling studies. Here, the measured amounts of leaf-deposited magnetic PM after roadside greening (‘tredge’) installation, and measured reductions in playground PM, particle number and black carbon concentrations demonstrate that air quality improvements by deposition can be achieved at the local, near-road, community/playground scale. PM deposition on the western red cedar tredge removed ~ 49% of BC, and ~ 46% and 26% of the traffic-sourced PM2.5 and PM1, respectively. These findings demonstrate that roadside vegetation can be designed, installed and maintained to achieve rapid, significant, cost-effective improvement of air quality by optimising PM deposition on plant leaves.

Wiggle-Matched Red Cedar from a Pre-Monumental Occupation at Kincaid Mounds, Illinois, USA

Wiggle-matched 14C dates anchored an 82-year floating cedar tree-ring chronology from the basal layer of a Mississippian Period mound to AD 1170 ± 9 (1σ) (outermost ring). Although numerical cross-matching showed preference for a temporal placement consistent with the 14C dates and visual matching, statistical scores were below critical thresholds for annual crossdating. Sapwood-corrected terminus post quem dates for the material, and their stratigraphic relationship with overlying dates, show that the earliest truncate mound construction at Mound 4 occurred after AD 1177. This date is informative for the timing of the transition of Kincaid to a major regional center and does not support an earlier hypothesis that Mound 4 was constructed in Early Kincaid times (AD 1050–1150). These results demonstrate the potential for archaeological collections to extend long tree-ring chronologies in the Midwestern U.S.

Experimental and computational study on the glowing ignition of wood

AbstractWildland Urban Interface (WUI) fires are a rapidly increasing hazard. Cellulosic materials play a key role in the spread of WUI fires. The physics controlling the glowing ignition of cellulosic materials is not well understood. In this work, an experimental and computational study of the glowing ignition of wood was performed. Eight wood specimens (Balsa, Red Cedar, Pine, Douglas Fir, Cypress, Redwood, Purpleheart, and Ipe) were tested under a cone calorimeter in the vertical configuration. Non‐uniform glowing propagation due to sample‐specific surface cracking was observed for all wood samples. The average temperature at the center of each sample was measured using an optical pyrometer. The inflection point in the pyrometer temperature‐versus‐time curve, which was identified by the maximum peak of the first temperature derivative, was used to identify the onset of glowing ignition. Additionally, a comprehensive model was applied to predict the glowing ignition. The comparisons between model predictions and experimental results showed that the surface temperature, the time to glowing ignition, and the glowing ignition temperature of medium‐density wood (ρ = 300–500 kg m−3) could be reasonably predicted. For lighter (e.g., Balsa, ρ = 150 kg m−3) and denser wood (e.g., Ipe ρ = 960 kg m−3), however, significant discrepancies were observed. Hence, further work is needed to understand the influence of wood density on the thermo‐physical and thermodynamic properties that can significantly affect glowing.

Late Woodland feasting and social networks in the lower Missouri River region

Feasting and its function among small scale societies have received little attention among many descriptive and theoretical studies of this activity. Evidence of feasting focused on large roasting pits by Late Woodland (AD 500–1000) hunter-gatherer-gardeners in the lower Missouri River region on the eastern edge of the Great Plains is presented. Two features at the Quixote site and lipid analyses of stones from them attest single events focused on roasting deer and fish, and perhaps ritual use of red cedar and ceramic pipes. Such features at other Late Woodland sites in the LMRR, specifically the Valley Falls locality of the Delaware River valley in northeastern Kansas, are reinterpreted. Burned stone features at Middle Woodland sites differ and suggest pit roasting was a practice of dispersed Late Woodland groups. Feasting for solidarity forged a social network based on reciprocity, not competition, to mitigate food insecurity among diffuse, low level food producers.

Influence of Climate and Coastal Flooding on Eastern Red Cedar Growth along a Marsh-Forest Ecotone

Coastal forests in the Mid-Atlantic region are threatened by sea level rise through chronic and episodic salinization and hydrologic alterations, leading to inland marsh migration and the occurrence of ghost forests. This study uses dendrochronology to explore the impact of rising sea level on the annual growth of Juniperus virginiana (the Eastern red cedar) at the St. Jones component of the Delaware National Estuarine Research Reserve in Dover, DE. Chronologies from low and high elevations were developed, and a difference chronology (high–low) was generated. A rapid field assessment of tree stress indicated greater stress in low elevation trees, and low elevation soil tests showed higher soil moisture and salt content compared to samples from high elevation. Ring width indices were analyzed in relation to water level, precipitation, the Standardized Precipitation Evapotranspiration Index, and temperature, with Pearson’s correlation analysis. Trees growing at low elevation showed greater climate sensitivity and responded favorably to cool, wet summers. Over time, correlations between growth and climate variables decreased, while negative correlations with tidal water level increased—a pattern that presented nearly a decade earlier in the low elevation system. Given the widespread distribution of the Eastern red cedar and its sensitivity to changes in sea level, this species may be particularly useful as a sentinel of change in coastal landscapes as sea levels rise.

Stages and time-scales of ignition and burning of live fuels for different convective heat fluxes

Wildland fires impact ecosystems and communities worldwide. Many wildfires burn in living or a mixture of living and senescent vegetation. Therefore, it is necessary to understand the burning behavior of living fuels, in contrast to just dead or dried fuels, to more effectively support fire management decisions. In this study, the ignition and burning behaviors of needles placed in convective heat flux were evaluated. The species included longleaf pine (Pinuspalustris), Douglas-fir (Pseudotsuga menziesii), western red cedar (Thuja plicata), ponderosa pine (Pinus ponderosa), western larch (Larix occidentalis), pacific yew (Taxus brevifolia), white spruce (Picea glauca), and sagebrush (Artemisia tridentate). The ignition and burning behaviors were related to live fuel moisture content (LFMC), pilot flame temperatures, and convective heat fluxes. The different stages of ignition and burning were captured using high-speed imaging. In general, four burning stages can be observed: droplet ejection and burning, a transition stage, flaming combustion, and smoldering combustion. Ejection and subsequent burning of droplets can occur prior to sustained flaming ignition only in live fuels. For some species (e.g., longleaf pine, ponderosa pine, white spruce), droplet ejection and burning can reduce ignition times relative to dried fuel with lower LFMC. In general, the transition stage tends to take longer than the flaming and droplet stages (when these occur). During the transition stage, the fuels are heated, and pyrolysis occurs. Time-scales to ignition and the different stages of ignition and burning vary more among live fuels than dead and dried fuels. This conclusion indicates that other parameters, such as chemical composition and structural morphology of the fuel, can significantly influence the burning of live fuels.