Red Betel Research Articles

Antibacterial activity of fractions (chloroform, ethyl acetate and methanol) red betel leaf ethanol extract (Piper crocatum) against Streptococcus pyogenes bacteria

Antibacterial activity of fractions (chloroform, ethyl acetate and methanol) red betel leaf ethanol extract (Piper crocatum) against Streptococcus pyogenes bacteria

Aktivitas Antibakteri Minyak Sirih Merah (Piper Crocatum Ruiz & Pav.) Dalam Bentuk Sediaan Emulsi dan Mikroemulsi

Minyak Sirih Merah (MSM) diketahui mempunyai aktivitas sebagai antibakteri dan dapat dibuat dengan cara distilasi air dan uap dari daun sirih merah (Piper crocatum Ruiz & Pav.). Tujuan dari studi ini adalah untuk membandingkan aktivitas antibakteri dari MSM apabila diformulasi dalam bentuk sediaan yang berbeda (yaitu emulsi dan mikroemulsi). MSM dalam bentuk emulsi dibuat dengan cara mencampur 0,125 g MSM, 0,875 g paraffin cair, 0,125 g tween-80, 0,375 g span-80 (HLB 7), dan 3,500 g air. MSM dalam bentuk mikroemulsi dibuat dengan mencampur 0,125 g MSM, 0,125 g paraffin cair, 0,625 g tween-80, 0,625 g PEG-400, dan 3,500 g air. Aktivitas antibakteri terhadap Escherichia coli ATCC 25922 dilakukan dengan menggunakan metoda mikrodilusi dengan 1% w/w konsentrasi menggunakan reader ELISA pada 570 nm. Data kemudian dianalisis untuk membandingkan persentase penghambatan pertumbuhan mikroba. Hasil penelitian menunjukkan bahwa hambatan bakteri dari MSM dalam bentuk emulsi adalah 17,45±5,9% sedangkan dalam bentuk mikroemulsi 49,58±3,27%. Kesimpulannya adalah aktivitas antibakteri MSM dalam bentuk mikroemulsi lebih baik dari bentuk emulsi.

Efektivitas Formulasi Ekstrak Daun Sirih Merah (Piper crocatum) Dengan Ekstrak Daun Pandan Wangi (Pandanus amaryllifolius Roxb.) Terhadap Hiperglikemia Serta Histopatologi Pankreas Mencit

Diabetes mellitus (DM) is metabolism diseases characterized by hyperglycemia and change to the cell histophatology structure of pancreas. Red betel leaves (Piper crocatum) and fragnant pandan leaves (Pandanus amaryllifolius Roxb.) has compound metabolic secondary that efficacious as anantidiabetic. The aims of the study were seek the best extract formulations of red betel leaves (EDSM) and extract of fragnantpandan leaves (EDPW) to lowering blood glucose level, repair the damage of islet langerhans, and comparing the effectiveness with metformin. The research was drafted by post test only control group design using male mencit (Mus musculus) for 31 days. In this research used complete random design with 14 treatment groups, they are normal group, DM group, metfomin group, EDPW 100% group, EDSM 10%+ EDPW 90% group, EDSM 20% + EDPW 80% group, EDSM 30% + EDPW 70% group, EDSM 40% + EDPW 60% group, EDSM 50% + EDPW 50% group, EDSM 60% + EDPW 40% group, EDSM 70% + EDPW 30% group, EDSM 80% + EDPW 20% group, EDSM 90% + EDPW 10% group, and EDSM 100% group. Based on in vivo bioassay showed that metformin have a lot of lowering blood glucose levels (p 0,05) and better in improving islet langerhans tissue damage caused by alloxan diabetogenic compound.

Pertumbuhan Stek Batang Sirih Merah (Piper crocatum Ruiz.) Setelah Perendaman Dengan Ekstrak Bawang Merah (Allium cepa L.) Dan Air Kelapa (Cocos nucifera L.)

The Red betel (Piper crocatum Ruiz.) is one of the types of plants that can be used as a medicinal plant. The Red betel plant can be propagated through cuttings, but the ability of the Red betel leaves to stem cuttings rooted very low. The purpose of this research is to know the influence of the concentration of soaking onion extract and coconut water towards the growth of the stem cuttings of red betel. This study used a randomized complete design (RAL) factorial with two levels, namely the first factor extract of onion (B) that consists of 4 concentrations, namely control (0%), B1 (2,5%), B2 (4%), and B3 (5.5%). The second factor is cocnut water (K) consisting 0f 4 concentrations of control (0%), K1 (15%), K2 (20%) and K3 (25%), retrieved 16 combination treatments 3 replications to obtain 48 units of the experiment. The results showed that the combination of onion extract 5.5% + 25% coconut water shows most good for the number of leaves, plant height, wet weight of heading, the heading dry weight, root weight of wet and dry weight of roots.

The effectiveness of red betel leaf (Piper crocatum) extract against periodontal pathogens

Introduction: Red betel (Piper crocatum) has long been known as one of herbs that has antibacterial properties as it contains several useful compounds such as essential oils, flavonoid, alkaloids and tannins.  The objective of this study is to learn more about the effectiveness of red betel leaf extract based on in vitro test against periodontal pathogen bacteria such as Aggregatibacter actinomycetemcommitans (Aa) and Porphyromonas gingivalis (Pg).Method: This study was conducted using diffusion method that has Kirby-Bauer test sensitivity. The experiment was performed eight times on four treatments consisting of 10% DMSO as negative control and 2.5%, 5% and 10% of red betel leaf extract. The sterile paper disc was soaked into sterile water and red betel leaf extract at each concentration before evaporation inside the incubator.  After that, the dry paper disc was placed in the Nutrient Agar (NA) that had previously been inoculated and incubated for 24 hours at 37° Celsius. ANOVA test was used to compare inhibition zone in four group intervention.Result:  The result of the study shows that the 2.5%, 5% and 10% concentration of red betel leaf extract respectively could inhibit the growth of bacteria Aa and Pg. The result of the study also confirms that the 10% red betel leaf extract concentration created the largest inhibition zone (10.5786 mm for bacteria Aa and 10.7638 mm for bacteria Pg).Conclusion: Red betel leaf extract, particularly at 10% concentration is significantly effective in inhibiting the growth of Aa and Pg bacteria.Â

PERBEDAAN DAYA HAMBAT ANTIBAKTERI ANTARA EKSTRAK DAUN SIRIH MERAH (Piper crocatum) DAN EKSTRAK DAUN SIRIH HIJAU (Piper betle L.) TERHADAP BAKTERI Streptococcus mutans

Background: Red Betel ( Piper crocatum ) and green betel ( Piper betle L.) is believed to have efficacy preventing dental caries disease caused by the bacterium Streptococcus mutans . Purpose: Research purpose is to review describes different inhibitory extract of red betel leaf and leaf green betel Against bacteria Streptococcus mutans . Materials and Methods: Operations Research is an experimental by design post test only group design. Sample Research : is a pure culture of Streptococcus mutans bacteria in the laboratory of Microbiology, Faculty of Pharmacy, University Muslim Indonesia. Analitical statistics to be done using One Way ANOVA. Furthermore, the Post Hoc multiple comparison test for review red betel Differences And green betel inhibit bacteria. Results: Based on the statistical test showed that the zone of inhibition power of 4 Different the concentration (0.1%, 1%, 10%, 100%) As well as having a significant difference between the two TYPE betel leaf against bacteria Streptococcus mutans . Conclusion: The findings of the data analysis can be concluded that the green betel leaf showed inhibitory better than red betel leaf against bacteria Streptococcus mutans .

A REVIEW ON HERBAL COSMETICS IN INDONESIA

Nowadays, Herbal cosmetic is growing rapidly as most women prefer natural products rather than chemicals products for their personal care. Herbal cosmetic contains natural nutrients to improve and provide consumers satisfaction due to relatively fewer side effects compared to synthetic cosmetics. Indonesia is one of the biggest supplier’s countries for herbal raw materials in cosmetic products. Many plants are available naturally as well as different uses that can be made as basic ingredients for cosmetic preparations, such as onion dayak bulb, kemuning leaf, pegagan, kecombrang, red betel, pecan, and sweet root. This review aimed to provide information on plants in Indonesia based on the phytochemicals contents that can be formulated into various categories of cosmetic preparations, such as skin care, hair care, anti-aging, skin whitening, and antioxidant.

The comparison of two neolignans isolated from red betel leaf and its extract against macrophage phagocytic activity, the level of AST, and histopathological features of the liver in mice

A single active compound isolated from a plant extract may show a lower, equal, or greater activity compared to its extract. The two neolignans (Pc-1 and Pc-2) has been isolated from red betel (Piper crocatum Ruiz & Pav.) leaf. The aim of this study was to compare the phagocytic activity of macrophage and histopathological feature in mice treated with Pc-1, Pc-2 and its Pc-extract. Listeria monocytogenes was used to induce Balb/c mice immune response. Peroral administration of Pc-1 and Pc-2 and red betel leaf methanolic Pc-extract were carried out. The phagocytic effect was determined by macrophage phagocytosis and nitric oxide (NO) assays. The morphological feature of liver and kidney were observed using light microscope. The level of apartate transaminase (AST serum) and alanine transaminase (ALT serum) were also measured from the blood serum before and after L. monocytogenes infection. The results shows that the macrophage phagocytic activity of given 450 mg/kg body weight Pc-extract was equal to 5 mg/kg body weight of Pc-1 and Pc-2. The activity pattern of percentage phagocytosis, index phagocytosis, and efficiency phagocytosis of Pc-extract, Pc-1, and Pc-2 were similar, as well as the NO production. A certain dose of Pc-extract and both isolated compound reduced the level of AST while there were no effect on ALT level. There were no histopathological features differences in the liver after treated with Pc-extract and Pc-1. However, Pc-2 treatment caused hydropic degeneration on liver. Therefore it can be concluded that there were equal activity between Pc-extract and the isolated compound in certain dose. The Pc-extract and isolated compound reduced the level of AST while there were no effect on ALT. The only Pc-2 affected the histopathological features of the liver.

EVALUATION OF HEPATOPROTECTIVE EFFECT OF ETHANOLIC EXTRACT FROM RED BETEL (PIPER CROCATUM RUIZ AND PAV.) LEAVES

Objective: The objective of this study is to identify the effect of red betel (Piper crocatum Ruiz and Pav.) leaves ethanolic extract on hepatoprotective effect on CCl4-induced liver damage in rats.Methods: Standard phytochemical screening method was done for preliminary phytochemical screening. Hepatotoxic rats were induced with CCl4 and treated with ethanolic extract of red betel leaves. The hepatoprotective test was divided into two groups (curative and preventive test). In the curative test, the rats which have been induced with CCl4 were given ethanol extract 600 mg/kg bw each day for 15 days. In the preventive test, the induced rats were given ethanol extract 600 mg/kg bw each day and CCl4 each 4 h for 45 days. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) parameters were also measured.Result: The phytochemical screening showed an ethanolic extract of red betel leaves which contain a lot of phytochemical compounds. The result of hepatoprotective study showed that the ALT, AST, and ALP registered a significant (p<0.05) alteration in CCl4treated rats.Conclusion: The ethanolic extract of red betel leaves had the curative and preventive test.

Potensi Salep Ekstrak Daun Sirih Merah Terhadap Profil Eritrogram Sebagai Terapi pada Luka Miasis (THE EFFECT OF RED BETEL LEAF EXTRACT OINTMENT IN ERYTHROGRAM PROFILES AS THERAPY IN MYASIS WOUND)

The objectives of this research was to examine the efficacy of red betel extract ointment to eritrogram profiles in infected sheep myiasis. Fifteen female sheeps were divided into five treatments, which were sheep without wound, treated with asuntol 2%, without any treatment,with red betel leaf extract ointment 2% and 4 %. Three incision wounds were made on the sheep’s back, 50 larvae given to every wounds to four treatment, except the healthy sheep (K). Blood was taken on the 0, 3th and 7th to determine erythrocytes counts, hematocrit values (PCV) and hemoglobin levels (Hb). The result showed that oitment of red betel leaf extract do not change the erythrogram profiles. The administration of red betel leaf extract ointment 4% has better eritrogram profiles than 2% Oitment of red betel leaf extract can be used as myasis therapy.

Chemical Compound Of Liquid Smoke Derived From Leaf Of Piper betle L And Piper crocatum Ruiz & Pav

Liquid smoke made from green betel leaf (Piper betle L) and red betel leaf (Piper crocatum Ruiz & Pav) has been made by pyrolysis. To determine the allegations of the chemical compounds contained in these two liquid smoke, they were tested by Gas Chromatography–Mass Spectrometry (GC-MS) method. Based on the test results it was found that there were 7 suspected compounds in the liquid smoke of green betel leaf and 30 compounds in red betel liquid smoke. The main compounds contained in the liquid smoke of green betel leaf are Isamoxole as the anti-allergic, 5,10-Diethoxy-2,3,7,8-tetrahydro-1H, 6H-dipyrrolo [1,2-a; 1', 2'-d ] Pyrazine as an antifungal and 3,5-Dimethoxyphenol has the potential to be antibacterial while in red betel leaves are 4- (1H-1,3-Benzodiazol-2-yl) phenol and Benzene, 1,2,3-trimethoxy-5- (2 -propenyl) - Bioactivity potential of the two compounds has not been found.

The Benefits of Red Betel Leaf Extract for Perineal Wound Healing in BPM Rini District Kediri

Red Betel Leaf contains substances which are effective to treat the wounds. Therefore, it is important to conduct a research to ascertain the difference of healing duration, infection marks, perenium wound pain using red betel leaf 2x/day, red betel leaf extract 1x/day compared with using povidone iodine 10%. This resarch design is in a true experiment with factorial design. The samples are 18 postpartum mother, divided into 3 groups and each is 6 postartum mother, and are chosen with simple random sampling method. The variables that are measured in this research are the healing duration, infection marks, perineum wound pain. This research is also conducted using statistic test one way anova with post hoc as the post test. The result shows that the alpha score is 0.002 which means there is a significant difference of injury care using red betel leaf 2x/day, red betel leaf extract 1x/day with povidone iodine 10% to the duration of healing. The alpha score results at 0.003 shows the significant difference difference of injury care using red betel leaf 2x/day, red betel leaf extract 1x/day with povidone iodine 10% to the infection marks. The alpha score results at 0.021 shows the significant difference difference of injury care using red betel leaf 2x/day, red betel leaf extract 1x/day with povidone iodine 10% to the wound pain. The perineum injury care of postartum mother using red betel leaf extract 2x/day is more efficient and neither does infection marks nor wound pain is found.

PENGARUH EKSTRAK DAUN SIRIH MERAH (Piper crocatum Ruiz & Pav.) TERHADAP GLUKOSA DARAH MENCIT (Mus musculus L.) JANTAN YANG DIINDUKSI SUKROSA

Patients withdiabetes mellitus(DM) continues to grow becauseprosperityandpeople's lifestyles.Treatment of diabetesoften useinjectionsof insulinandoralantidiabeticdrugs. Thetreatmenthas no side effects. Therefore, it isnecessary to findeffective drugsusing plantsthatred betelleaf(Piper crocatumRuiz&Pav.). Red betel leafcontains flavonoidswhichare antioxidants. This study aimsto determine the effectand dose ofextractof redbetel leaf(Piper crocatumRuiz&Pav.) The mosteffectiveagainstbloodglucosein mice(Mus musculusL.) maleinducedsucrose.This study was an experimental study. The research was conducted in October 2015 in the Division of Laboratory Animal and Zoology Department of Biology, State University of Padang. The subject of research in the form of mice (Mus musculus L.) males totaled 24 tails. The design used was completely randomized design (CRD) with 6 treatments and 4 repetitions. The treatment is given as follows: treatment I: the diabetes control without any treatment given, treatment II: as a negative control (sucrose 3 g/kg bw), treatment III: sucrose+suspension of red betel leaf extract (dosage 0,7 g/kg bw), treatment IV: sucrose+suspension of red betel leaf extract (dosage 1,4 g/kg bw), treatment V: sucrose+suspension of red betel leaf extract (dosage of 2,1 g/kg bw) and treatment VI: sucrose+suspension extracts red betel leaf (dosage 2,8 g/kg bw).The results showed that the extract of red betel leaf (Piper crocatum Ruiz & Pav.) at a dose of 0,8 g/kg bw 1,4 g/kg bw 2,1 g/kg bw and 2,8 g/kg bw can lowers blood glucose in mice. However, the most appropriate dose in lowering blood glucose in mice (Mus musculus L.) at 2,8 g/kg bw in mice.

Aktivitas Mukolitik Kombinasi Ekstrak Etanol Daun Kemangi dan Ekstrak Etanol Daun Sirih Merah

Cough is a protective physiological mechanism that is useful for removing and clearing the respitory tract from sputum and foreign particles that can cause infection. Treatments on cough disorders using synthetic drugs are known to cause side effects. Therefore, it is important to develop new alternative cough drug derived from medicinal plant which has less side effects. Basil leaves ( Ocimum sanctum L. ) and red betel leaves ( Piper cocatum Ruiz and Pav) have been used traditionally to treat cough. Thus, the purpose of this study was to determine the mucolytic activity and the effective concentration of combination of basil and red betel leaves ethanol extract. The mucolytic activity was performed by measuring the decrease of viscosity of artificial sputum from the cow intestinal mucosa upon extracts treatment with various concentrations. Results shows that ethanol extract of basil leaves or ethanol extract of red betel leaves can reduce viscosity of artificial sputum at dose of 0.5% each. Moreover, combination of these extracts synergistically decreases the viscosity. Combination of ethanol extract of basil leaves and red betel can be an alternative medicine for cough treatment.

The Utilization of VCO (Virgin Coconut Oil) in Manufacturing of Solid Soap with Red Betel Leaf Extract Addition (Paper Crotum Ruiz &Pav)

Soap is a compound of sodium or potassium with fatty acids from vegetable oils or solid animal fats, soft or liquid, and foamy. Considering the potential of VCO as the raw material for making soap and supported by the benefits of red betel leaves, then this research is done by making solid bath soap from VCO which is supplemented with Red betel leaf extract. The purpose of this research is to make solid soap from VCO with an extract of red betel leaf based on SNINo.06-3532199. Analyzing VCO oil, which is used for the manufacture of soap, consists of analysis of saponification figures, Iodine number and peroxide number. Has made solid soap from VCO oil with Red betel leaf extract. From the five quality standards established under SNI 06-3532-1994 only two quality standards that can be done that is water quality and the amount of acidity. The percent of water quality obtained is 10% meanwhile the amount of acidity obtained is 9,32%. According to the data, it can be concluded that the solid soap made was not fulfill SNI 06-3532-1994.

Effect of the Extraction Solvent Polarity and the Ratio of Feed and Solvent on the Phytochemical Content and Antioxidant Activity of Red Betel Leaves (Piper crocatum)

Red betel leaf (Piper crocatum) is a plant growing in tropical areas and previously known as an ornamental plant, but then later used as medicinal plant. Previous researches found that red betel leaves contain bioactive compounds such as alkaloids, flavonoids, tannins, and many more that have potential to be used as antioxidant. The extractions were carried out using variations of solvent types (ethanol, water, and ethyl acetate) and feed to solvent (F:S) ratios in g/ml (F:S=1:20, F:S=1:25, F:S=1:30). The best conditions from this research were the usage of ethanol as the solvent. In addition to its ability to extract the compounds potential as antioxidant and F:S ratio of 1:30, it could give highest yield of extract. Variation of solvent type and variation of F:S have significant effect on the value of antioxidant activity (IC50) of the red betel leaf sample.

PENGARUH EKSTRAK DAUN SIRIH MERAH (Piper crocatum Ruiz & Pav.) TERHADAP GLUKOSA DARAH MENCIT (Mus musculus L.) JANTAN YANG DIINDUKSI SUKROSA

Patients withdiabetes mellitus (DM) continues to grow because prosperity and people's lifestyles.Treatment of diabetes often usei njections of insulin and oral antidiabetic drugs. Thetreatment has no side effects. Therefore, it is necessary to find effective drugs using plants thatred betel leaf (Piper crocatum Ruiz & Pav.). Red betel leaf contains flavonoids which are antioxidants. This study aims to determine the effect and dose of extract of red betel leaf (Piper crocatum Ruiz & Pav.) The most effective agains blood glucose in mice(Mus musculusL.) male induced sucrose.This study was an experimental study. The research was conducted in October 2015 in the Division of Laboratory Animal and Zoology Department of Biology, State University of Padang. The subject of research in the form of mice (Mus musculus L.) males totaled 24 tails. The design used was completely randomized design (CRD) with 6 treatments and 4 repetitions. The treatment is given as follows: treatment I: the diabetes control without any treatment given, treatment II: as a negative control (sucrose 3 g/kg bw), treatment III: sucrose+suspension of red betel leaf extract (dosage 0,7 g/kg bw), treatment IV: sucrose+suspension of red betel leaf extract (dosage 1,4 g/kg bw), treatment V: sucrose+suspension of red betel leaf extract (dosage of 2,1 g/kg bw) and treatment VI: sucrose+suspension extracts red betel leaf (dosage 2,8 g/kg bw).The results showed that the extract of red betel leaf (Piper crocatum Ruiz & Pav.) at a dose of 0,8 g/kg bw 1,4 g/kg bw 2,1 g/kg bw and 2,8 g/kg bw can lowers blood glucose in mice. However, the most appropriate dose in lowering blood glucose in mice (Mus musculus L.) at 2,8 g/kg bw in mice.

Red Betel Leaves Methanolic Extract (Piper crocatum Ruiz & Pav.) Increases Cytotoxic Effect of Doxorubicin on WiDr Colon Cancer Cells through Apoptosis Induction

Doxorubicin is a chemotherapeutic agent that causes a lot of side effects in high doses. Thus, combination with a co-chemotherapeutic agent which can increase its toxicity on cancer cells, is needed to reduce its therapeutic dose. Red betel leaves (Piper crocatum Ruiz & Pav.) have been known to contain flavonoids and alkaloids that have anticancer activity. This study was conducted to determine the cytotoxic effect and apoptosis induction of red betel leaves methanolic extract (RBM), doxorubicin (dox) and the combination of them on WiDr cells as model of colon cancer. RBM was extracted by soxhlet method using methanol. Cytotoxicity assay was performed using MTT assay for both single and combination treatments for 24 hours to determine IC50 and CI as their parameters. Apoptosis induction was analyzed by double staining method using ethidium bromide-acridine orange staining. Treatment of RBM and dox on WiDr cells for 24 hours showed cytotoxic activity with IC50 100 μg/mL and 1.6 μM respectively. Combination of RBM and dox performed synergism effect with CI<0.9 (p<0.05). Combination of RBM (12.5 μg/mL) and dox (0.4 μM) increased the number of apoptosis cells compared to each single treatment. Based on this study, it can be concluded that red betel leaves methanolic extract is potential to be developed as a co-chemotherapeutic agent of doxorubicin on colon cancer but still need further study to disclose the underlying molecular mechanisms.Keywords : Red betel leaves (Piper crocatum Ruiz & Pav.), doxorubicin, WiDr cells, co- chemotherapeutic agent

Methanolic Extract of Red Betel Leaves (Piper crocatum Ruiz & Pav) Perform Cytotoxic Effect and Antimigration Activity toward Metastatic Breast Cancer

Breast cancer is one type of cancer with a high mortality rate due to its metastatic property. Red betel leaves (Piper crocatum Ruiz dan Pav) has been known as herbal medicine containing biophenolic, such as apigenin and luteolin derivatives which has cytotoxic activity toward cancer cells. This study is intended to explore the inhibitory effect of Piper crocatum leaves methanolic extract (PCM) on cell proliferation and migration by using 4T1 cells as model of metastatic breast cancer. By using MTT assay, PCM performed cytotoxic activity in a dose dependent manner with IC50 value of 120 μg/mL. Wound healing assay revealed that migration inhibitory activity of PCM on 4T1 cells at the concentration of 30 µg/mL. In conclusion, PCM perform cytotoxic effect and antimigration activity toward metastatic breast cancer cells.Keywords : breast cancer cells, Piper crocatum Ruiz & Pav, cytotoxic, cell mgration

Antibacterial Effect of Red Betel (Piper crocatum Ruiz and Pav.) Extract in Combination with Vancomycin against Staphylococcus aureus

Objectives: The current study was performed to show the antibacterial effect of red betel ( Piper crocatum Ruiz and Pav.) in combination with vancomycin against Staphylococcus aureus. The reduction of S. aureus susceptibility to vancomycin was reported in 1997. The combination of isolated antibacterial compounds from plant with antibacterial drug were expected to increase antibacterial activity resulted in the reduction of resistance. Methods: The antibacterial effect of red betel extract and vancomycin separately and the combination of both materials were evaluated based on the zone of inhibition diameter using agar diffusion method. The interaction effect of extract and antibiotic combination was performed using microdilution checkerboard method. Results: There was an antibacterial effect of red betel extract started at the concentration of 150 mg/ml. The combination of red betel extract with vancomycin showed significantly greater inhibition growth of S. aureus compared to those of red betel or vancomycin alone. The addition of red betel extract to vancomycin reduced Minimum Inhibitory Concentration (MIC) vancomycin to be a-4 fold reduction against S. aureus . Moreover, the Fractional Inhibitory Concentration Index (FICI) of red betel extract and vancomycin combination was 0.3125. Conclusion: There was a synergistic effect of the red betel extract in combination with vancomycin against S. aureus . It is the potential result for the future research on the infectious treatment of S. aureus resistant to vancomycin. Key words: Piper crocatum , Vancomycin, Synergistic antibacterial, Staphylococcus aureus .