An experimental study was performed to investigate the effects of nozzle spacing between twin rectangular jets on the non-linear momentum interference and spatial evolution of large-scale flow structures. Five test cases of different spacing ratios (S/de = 1.8, 2.8, 3.7, 5.5 and 7.3) were examined. Two-point correlation, joint probability density function and swirling strength were used to characterize the large-scale structures. Turbulent/non-turbulent interface along the inner and outer shear layers were investigated. The results showed that due to the interactions in the merging region, the spatial correlation of streamwise velocity fluctuations was considerably lower along the inner shear layer than along the outer shear layer. As a result of the mixing process, the swirling strength of retrograde vortices generated along the inner shear layer was also lower than that of the prograde vortices. Along the symmetry line, longitudinal and transverse integral length scales increased linearly as the downstream distance from the jets increases. For the longitudinal length scale, the slope of the linear region was independent of the spacing ratio, while for the transverse length scale, the slope increases as the spacing ratio increases. For cases of S/de < 3.7, due to the suppression effects of the adjacent jet, the jump in the conditionally-averaged mean streamwise velocity profile around the turbulent/non-turbulent interface was lower for the inner shear layer compared to the outer shear layer.