This paper presents a quenching bath contamination characterization method using non-destructive acoustic emission (AE) testing. A 4-channel continuous recording AE setup with container side-mounted AE piezoelectric sensors is proposed. Cylindrical steel specimens are quenched in tap water, deionized water, 5% salt solution or 5% detergent solution. The AE peak amplitude over time characteristics are recorded and analysed. A referential signal polynomial fit with a specific confidence interval is established based on the model fit statistics. A comparison of the recorded AE signals and the referential fit is presented. The quenching bath property deviations are compared using outlying AE peak amplitude signal packets and a maximum out of bounds acceptance level is determined. Specimen microstructural analysis is performed in order to evaluate the correlation of microstructure and AE characteristics. The experimental results confirm the possibility of detecting quenching bath contamination or property deviations with regards to a predetermined referential AE signal, thus paving the way for possible industrial application.