An experimental investigation was carried out on the flapping motion of a turbulent reattaching shear layer downstream of a two-dimensional backward-facing step. The Reynolds number was 2.0 × 104, based on the free-stream velocity and the step height. The aim of this study is to analyze the flapping motion, which is featured unsteadiness of the reattaching shear layer, and its interaction with the recirculation region. High-resolution planar particle image velocimetry was used to measure the separated and reattaching shear layer in a horizontal-vertical plane. The velocity vector fields have shown the reattaching shear layer considerably flaps upwards and downwards as much in scale as approximately one step height from the middle part of recirculation region to the reattachment area. As a result, the recirculation region varies in size and the reattachment point shifts upstream and downstream. By applying singular value decomposition and proper orthogonal decomposition, the flapping motion is decomposed into multiple spatial modes, each of which represents interactions between the reattaching shear layer and recirculation region. In particular, the unsteady movement of the reattachment point is highly correlated with the flapping motion, and so is the maximum reverse flow. As a result, the flapping motion contributes substantial parts of the Reynolds shear stress and turbulent kinetic energy within the shear layer in the latter half of the reattachment length.