Due to the virtues of low-cost and high positioning accuracy, Single-Frequency Precise Point Positioning (SF-PPP) is becoming a prospective technique. However, SF-PPP is not as widely used as dual-frequency and triple-frequency PPP at present, owing to the effect of ionospheric delay residuals after model rectification. In recent years, with the evolution of multi-constellation Global Navigation Satellite Systems (multi-GNSS, i.e., GPS, BDS-2, and BDS-3), it has become possible to obtain credible and continuous positioning results using SF-PPP. However, such performance would be significantly degraded in challenging environments (i.e., boulevards, tunnels, and tall buildings). Under these circumstances, GNSS signals are obstructed, and it is difficult to provide sufficient observations for SF-PPP. Therefore, the Inertial Navigation System (INS) is employed to promote the positioning performance of SF-PPP. The PPP/INS integration is regarded as one of the most efficient approaches in GNSS-denied environments. To satisfy the request of supplying real-time positioning information, the Real-Time Services (RTS) of the International GNSS Service (IGS) provide real-time precise orbit and clock products for globally distributed users through the internet. In this paper, a real-time GPS/BDS-2/BDS-3 SF-PPP and INS tight integration model is proposed, and it is assessed using the data gathered by vehicle and real-time products afforded by CAS (Chinese Academy of Sciences), GFZ (Deutsche GeoForschungsZentrum), and WHU (Wuhan University). The outcomes illustrate the following: (1) GPS + BDS SF-PPP/INS can provide more accurate and continuous positioning solutions compared with those of GPS + BDS SF-PPP, with improvements of 52.8%, 31.1%, and 42.8% in the north, east, and vertical components, respectively. (2) In general, the orbit and clock products’ accuracies in terms of GPS afforded by the three analysis centers are consistent with each other. For BDS, the orbit product from WHU is more accurate compared to those of CAS and GFZ. However, the accuracy of the clock product afforded by WHU is lower compared with those provided by the other two centers, especially for BDS-2 satellites. (3) The positioning accuracy in terms of Root Mean Square (RMS) values based on GFZ products are much higher than the results based on CAS and WHU products in the three directions.