In order to solve the problem of slotting optimization management with automated storage and retrieval system (AS / RS), the genetic algorithms of the slotting optimization process with stereo warehouse were discussed. The process of the slotting optimization was designed by the real-number coding. The mathematical models of the slotting optimization and cargo shipping time were built, and the simulation calculation of the models was done. The stability of the shelf and the efficiency of the accessing goods were significantly improved by the optimization simulation of the distribution of cargo space, after optimizing the storage order. The algorithms of the slotting optimization with stereo warehouse could effectively enhance the frequency of the stock in & out, increasing the revenue.