Reagent Method Research Articles

Intensification of wastewater purification of municipal solid waste landfills

The object of research is the wastewater of municipal solid waste landfills and the effectiveness of using a combination of various technological methods, in particular, a combination of reagent and biological methods to bring the quality of filtrate to regulatory requirements. In particular, a reduction in the negative impact of wastewaters of MSW landfills, which contain toxic organic substances, on the environment and human health is achieved. In the course of the research, a comparative analysis was performed of the effectiveness of using conventional and activated coagulant solution of aluminum sulfate. A certain dependence of the activation parameters and the efficiency of cleaning the filtrate of landfills are obtained. To achieve the aim set in the work, the chemical composition of the landfill filtrate was studied at different stages of operation and the parameters of the reagent wastewater treatment of landfill sites were optimized. And also studied the kinetics and revealed the laws of the coagulation process when the coagulant solution is activated. When conducting research, the proposed technology was tested, pilot tests were carried out and an analysis of the technical and economic efficiency of the technology implementation was given. When performing experimental studies, a set of methods for determining pH levels, COD (chemical oxygen demand), BOD (biological oxygen demand), etc. was used. High efficiency of using the reagent method in combination with biological treatment for filtration waters characteristic of the methanogenesis stage was proved. The proposed technology has a number of features, in particular, during the activation of the reagent solution, a magnetic field is applied, as a result of which a change in the structure of the solution and the formation of additional coagulation centers are observed. The paper presents the results of a study of the technological and economic efficiency of the proposed technology, in particular, it has been proven to reduce operating costs when introducing the considered technological scheme and the proposed hardware design. Compared with other known technologies that involve the stage of reagent cleaning, this will allow to intensify the coagulation process and reduce the estimated dose of coagulant by 25–30 %, without degrading the quality of cleaning.

CLEANING OF OIL-CONTAINING WASTEWATER

The regularities of changes in the composition of wastewater, methods of cleaning and technological schemes of the oil extraction industry and the processes of formation of wastewater of the oil extraction plant CJSC Samaraagroprompererabotka and their chemical and physical indicators are studied. The eff ectiveness of wastewater treatment by reagent methods using known, analog and new reagents, on a model solution was investigated. An analytical dependence describing the processes of increasing the effi ciency of new technological processes (discrete and binary) reagent purifi cation has been carried out. The economic effi ciency of the proposed methods for treating oil-fat-containing wastewater is determined.

Physicochemical, Qualitative and Quantitative Phytochemical Analysis of the Leaf and Bark of Bombax Ceiba L (Red Silk Cotton Tree)

Bombax ceiba L is a big deciduous tree found in tropical and subtropical regions of Asia, Africa and Australia. Conventional systems of medicine such as Ayurveda, Siddha and Unani have been highlighted the use of B. ceiba parts (bark, leaves and flower) for the treatment of manydiseases like hypertension, HIV infections, inflammation, catarrhal affection, ulcer, acne, gynecological disorders, fever, dysentery, algesia, hepatotoxicity, piles and urinary infections. The aim of the present study was to evaluate pharmacognostic, physicochemical, qualitative and quantitative phytochemical analysis of leaf and bark of B. ceiba collected from Bhopal region of Madhya Pradesh. The pharmacognostic studies out in terms of various investigations like organoleptic or morphological characters, microscopic or anatomical studies, physicochemical evaluations (loss on drying, ash value, extractive values and acid insoluble ash value). Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. The detail microscopy of bark revealed the presence of cork cell, lignified fibre, calcium oxalate crystals, xylem vessels. Physiochemical parameters such as percentage of foreign matters, ash values, loss on drying swelling index, extractive values were determined. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, alkaloids and glycosides. In the procedure of quantitative analysis of flavonoids and phenolic compound was carried out by aluminium chloride Folins and Ciocalteau reagent method .In this methodthe totalflavonoids content and phenolic content of B. ceiba ethanolic and aqueous barks extracts was found to be 6.272, 3.363 and 2.607, 1.607mg /100mg respectively and total phenolic and flavonoids content of B. ceiba ethanolic, aqueous leaves extracts was found to be 7.381, 4.590 and 3.200, 1.792 mg/100mg respectively. These information will also be helpful to differentiate B. ceiba from the closely related other species. The diverse array of phytochemicals present in the plant thus suggests its therapeutic potentials which may be explored in drug manufacturing industry as well as in traditional medicine.

Extraction, Qualitative and Quantitative Determination of Secondary Metabolites of Rumex Nepalensis Roots

In the Indian ayurvedic system of medicine, Rumex nepalensis Spreng. (Polygonaceae) commonly known as Nepal Dock has wide-spectrum therapeutic potencies and is extensively used for centuries in traditional medicine systems. They act as a possible food supplement and are largely used in pharmaceutical industry. Extracts and metabolites from this plant exhibits pharmacological activities including anti-inflammatory, antioxidant, antibacterial, antifungal, antiviral, insecticidal, purgative, analesic, antipyretic, anti-algal, central nervous system depressant, genotoxic, wound healing and skeletal muscle relaxant activity. Due to its remarkable biological activities, it has the potential to act as a rich source of drug against life threatening diseases. The aim of the present study is to examine Rumex nepalensis roots for phytochemical profile. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by Folins Ciocalteau reagent method and aluminium chloride method respectively. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids, fixed oil and fats. The total phenolics content of roots ethanolic extract was (1.658 mg/100mg), followed by flavonoids (1.048mg/100mg). The present study concluded that the crude extract of Rumex nepalensis is a potential source of various activates and this justifies its use in folkloric medicine.
 Keywords: Rumex nepalensis, Qualitative analysis, Quantitative analysis, TPC, TFC, Folins Ciocalteau

Qualitative and quantitative determination of secondary metabolites and antioxidant potential of Nymphaea nouchali flowers

In the Indian ayurvedic system of medicine, Nymphaea nouchali is used for the treatment of diabetes, inflammation, liver disorders, cutaneous diseases, blenorrhagia, urinary disorders, menorrhagia, menstruation problem, as an aphrodisiac, bitter tonic, antimicrobial agent and anti antihepatotoxic effect. The aim of the present study is to examine Nymphaea nouchali flowers for phytochemical profile, in vitro antioxidant activities. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by Folins Ciocalteau reagent method and aluminium chloride method respectively. The In vitro antioxidant activity of ethanolic extract of the flowers was assessed against nitric oxide, hydrogen peroxide assay using standard protocols. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids, fixed oil and fats. The total phenolics content of flowers ethanolic extract was (18.4 mg/100mg), followed by flavonoids (12.4mg/100mg). The activities of ethanolic flowers extract against nitric oxide and hydrogen peroxide were concentration dependent with IC50 values of 68.39 and 64.54 μg/ml respectively. The present study concluded that the crude extract of Nymphaea nouchali is a potential source of natural antioxidants and this justifies its use in folkloric medicine.
 Keywords: Nymphaea nouchali, Phytochemical, Antioxidant, Nitric oxide, Hydrogen peroxide, Phenols, Flavonoids

Phytochemical, elemental, antioxidant, antimicrobial and hypoglycemic studies of a mixed herbal product used for the management of diabetics

Medicinal plants are important sources of disease-preventing compounds, which are important for the treatment of various health challenges such as diabetes. On an aqueous extract of a herbal product (HP) used for the management of diabetes, total phenolic and flavonoid contents were determined by Folin-Ciocalteu reagent and AlCl3 method respectively. Microbiological evaluation was done by determining the total viable, yeast, mould and coliform bacteria count. The elemental analysis was carried out using atomic absorption spectrometer. The acute toxicity was done using Organization for Economic Cooperation and Development guideline while the hypoglycemic activity was evaluated using alloxan-induced diabetic rats. Flavonoids, saponins, alkaloid, cardiac glycoside, steroids and terpenoids were detected in the HP. Total flavonoid and phenolic contents obtained was 1.58±0.001mg/g quercetin equivalent and 10.84±0.003 mg/g gallic acid equivalent respectively. Heavy metals Fe and Zn were present while Cu, Cd, Cr and Pb were absent. Na and K were also present at concentrations of 3.90 and 2.20mg·kg−1 respectively. The total viable and coliform counts were found to be 1.34 x 105 and 9.0 x 104 cfu/g respectively while there was absence of mould and yeast in the HP. The LD50 of the HP was found to be above 5000 mg/kg. At dose of 125 mg/kg, the HP significantly (P<005) reduced glucose level to 143 mg/dL after 4 hours and to 123 mg/dL after 8 hours. The phytochemicals present, safety and the anti-diabetic activity justify the use of this HP in the management of diabetes.Keywords: Phytochemical screening; Elemental analysis; Herbal product; Diabetes

A cost‐effective shortcut to prepare organoselenium catalysts via decarboxylative coupling of phenylacetic acid with elemental selenium

An interesting decarboxylative coupling reaction of phenylacetic acid with elemental selenium was discovered and employed for the preparation efficient organoselenium catalysts for Baeyer–Villiger reaction and oxidative deoximation reaction. Compared with the traditionally used Grignard reagent method, the decarboxylative coupling reaction with selenium powders provides a shortcut for the preparation of organoselenium catalysts free of carcinogenic organohalide starting materials, toxic and odorous selenol intermediates and magnesium salt solid wastes. This may be helpful for reducing the cost of selenium catalysts to facilitate the application of organoselenium‐catalyzed green reactions in large‐scale production.

Development of Latent Fingermarks on Surfaces of Food-A More Realistic Approach.

Latent fingermark developing methods from food (mandarin, egg, banana, apple, potato, carrot, eggplant, onion, bell pepper, and tomato) surface has been studied by 10 methods, including powdering method, small particle reagent method, and cyanoacrylate fuming method. The foods were stored under two conditions (room temperature and refrigerator) before fingermark deposition and aged for 2, 24, 48, and 72h before fingermark development. In most of the food surfaces used in this study, cyanoacrylate fuming with 80% relative humidity was superior to the powder or small particle reagent methods. The quality of the fingermarks varied depending on the storage conditions before the fingermark deposition (in the refrigerator or at ambient temperature) and the time elapsed since the fingermark deposition. However, the storage conditions before the fingermark deposition and the time elapsed since the fingermark deposition did not show any correlation with the quality of the fingermark.

ANALYTICAL METHOD DEVELOPMENT FOR THE ESTIMATION OF DARUNAVIR BY DIAZOTIZATION AND COUPLING BY VISIBLE SPECTROPHOTOMETRY

Objectives: This proposed work describes simple and extraction free visible methods for the estimation of darunavir ethanolate (DNV) in bulk and tablet formulations. The methodology involves diazotization of DNV with nitrous acid followed by coupling with chromotropic acid (CA) (Method A)/ Bratton Marshall Reagent (Method B)/α-naphthol (Method C) to form colored products.Methods: All the methods were developed using a PerkinElmer (LAMBDA 25) UV–Visible spectrophotometer interfaced with UV Win lab software and 1 cm quartz cells. Results: Spectrophotometrically, DNV is estimated at 520 nm, 544 nm, and 464 nm for the reddish-pink color produced by CA, dark violet color with NED, and dark-greenish yellow with α-naphthol, respectively. The linear relationship was observed between absorbance and the corresponding concentration of drug in the range of 100–350 μg/mL, 10–100 μg/mL, and 10–60 μg/mL for Methods A, B, and C, respectively.Conclusion: The colorimetric methods were extensively validated as per the ICH guidelines. The developed methods were proven to be more accurate and precise by the statistical analysis.

Abstract 289: Relationship Between Myocardial Glycogen Concentrations and Amplitude Spectrum Area During Ventricular Fibrillation in a Rat Model of Cardiac Arrest and Resuscitation

Introduction: Myocardial high-energy phosphate (ATP) levels has been demonstrated correlating with amplitude spectrum area (AMSA) during ventricular fibrillation (VF) in previous experimental studies. In the present study, we investigated the relationship between AMSA and myocardial glycogen content (MGC),which can be used to reflect the status of myocardial energy metabolism indirectly during VF. Hypothesis: AMSA has a significantly correlation with MGC during VF in a rat model of cardiac arrest and resuscitation. Methods: Twenty male Sprague-Dawley rats weighing 350 to 450 g were utilized and randomized into two groups: VF and cardiopulmonary resuscitation (CPR) (VF/CPR group) or untreated VF (VF group). 5 mins of CPR was performed after 10 mins of untreated VF in VF/CPR animals. Amplitude spectrum area (AMSA) at VF 5, 10 and 15 mins were calculated from ECG signals. The rats’ hearts were quickly removed at the predetermined time of 15 min for determines the glycogen contents by the anthrone reagent method using a glycogen assay kit. Results: AMSA values significantly decreased during untreated VF in both VF and VF/CPR animals. However, much greater AMSA during CPR was achieved by the VF/CPR group in comparison with the VF group. There was a marked and negative relationship between AMSA at VF 15 min and MGC. (Figure). Conclusion: MGC was significantly and negatively correlated with AMSA during VF in this rat model of cardiac arrest and resuscitation. In clinical practice, we can use AMSA to reflect the state of myocardial energy metabolism indirectly. Figure The changes of AMSA and relationship between AMSA and glycogen content:(A) The change of AMSA between VF/CRP group and VF group;(B) The relationship between AMSA and glycogen content. AMSA, amplitude spectrum area; V, time of ventricular fibrillation; # p <0.05 vs. V4.

The reagent method of the hydrogeological wells regeneration

The paper presents the timeframe of the under-stream period of hydrogeological well operation. The causes of the hydrological wells flowrate reducing while long operation have been indicated. The methods of the hydrological wells and near-filters zone decolmatation have been described. The pulse-reagent well recovery has been shown to be the most widely used method. The filter colmatant composition has been given and the possible filter failure cause has been shown. Laboratory investigations have been done with a special equipment that can be seen on the drawing. A solution of sulfamic and triacetate acid, corrosion inhibitor, surfactant, sodium tripolyphosphate, adalic acid and water has been suggested as an invention. Using of these chemicals allowed to achieve the synergistic effect. A double swab-packer and a washing device for wells regeneration has been designed, its description and drawing have been given. The optimal time for the colmated filter regeneration has been indicated. Laboratory investigations results are given in the table. The chemic equipment used for chemicals transportation and keeping of the reagents has been described. The created solution for hydrogeological wells regeneration has been shown to be the optinal and its using efficiency is forty percent more than that of the known one. The created solution is recommended for hydrogeological wells regeneration in field works.

Increasing the water yielding capacity of natural water sediments

When carrying out water treatment processes, a significant amount of hydroxide sediments is formed, which have a low water-yielding capacity. In the paper, the sediment of sewage treatment facilities of water pipes, which is formed in the conditions of low turbidity and medium color of water in a water source, was investigated. The effect of seasonal variations in turbidity, color and temperature of the treated water on the specific resistance of sediment filtration is shown. The features of sediment formation in sedimentation tanks of various types are considered. It is shown that the conditions for the formation of sediment affect its properties. It is established that the return of washing water, realized on one of the blocks of main water treatment facilities, increases the turbidity of the water to be purified and leads to the formation of sediments that are easier to be conditioned. At the same time, the sediment, formed in two-tier sedimentation tanks, when they are conditioned, require large doses of reagents. To intensify the dewatering of sewage treatment facilities, reagent methods were investigated using lime and filler materials. The mechanism of lime action on hydroxide sediments is shown.

Reagent decontamination of liquid chrome-containing industrial wastes

Abstract The results of the research into the reagent method of liquid chrome-containing wastes decontamination at the developed trial-experimental installation are observed. The construction of double-stage reactor–reducer with active charging is presented. The perceptiveness of using iron (II) oxide and metal turnings for the utilization of chrome-containing waste with the obtaining of the sediment with certain structure and properties is demonstrated. The technological peculiarities of the iron turnings application as the reducing agent are considered. The influence of the active charging specific area and that of the temperature on the rate and completeness of the hexavalent chromium reduction reaction is defined. The installation was used at real industrial environment and its efficiency was proved.

Brown macroalgae from the Adriatic Sea as a promising source of bioactive nutrients

In the present investigation the acetone extracts of three brown marine macroalgae Dictyota dichotoma, Padaina pavonia and Sargassum vulgare were tested for antioxidant, antimicrobial and cytotoxic potential. The antioxidant activity was evaluated by free radical scavenging, superoxide anion radical scavenging and reducing power. P. pavonia extract showed more potent free radical scavenging activity (IC50 = 691.56 µg L−1) than D. dichotoma and S. vulgare extracts. Moreover, the tested extracts had effective reducing power and superoxide anion radical scavenging. The total content of phenol and flavonoid in extracts was examined using Folin–Ciocalteu reagent and aluminium chloride method and the obtained values were expressed as pyrocatechol equivalents, and as rutin equivalents, respectively. Further, the antimicrobial potential was determined by a microdilution method. Among the tested species, the extract of D. diichotoma showed the best antimicrobial activity with minimum inhibitory concentration values ranging from 0.156 to 2.5 mg L−1. Finally, the cytotoxic activity was tested using microculture tetrazolium test on the human colon carcinoma LS174 cells, human lung carcinoma A549 cells, malignant melanoma FemX cells and chronic myelogenous leukaemia K562 cells. The extract of D. dichotoma expressed stronger cytotoxic activity toward tested cell lines with IC50 values ranging from 9.76 to 50.96 µg L−1.

Reagent purification of the processing industry enterprises effluents

The article represents the results of research of the effluents purification by the reagent methods. The effluents were polluted by organic compounds of processing enterprises with small productivity. The analysis of pollution of the hydrosphere caused by the processing enterprises is carried out and the promising methods of cleaning wastewater from pollution are given. As an object of research, the streams of the production site of the fruit and vegetable juices of the Buzky Canning Factory were used. As a reagent-oxidant, sodium hypochlorite was studied. The mechanism of purification of contaminated wastewater with sodium hypochlorite is considered in detail. The task of experimental studies was to determine the flow of sodium hypochlorite where wastewater treatment is carried out to a level lower than that regulated by standards. The criterion for cleaning efficiency was two indicators of water quality: chemical oxygen consumption (COC) and dry residue. The results of the conducted studies allow us to conclude that the use of sodium hypochlorite for purification of fruit and vegetable juice from organic pollutants is promising. It is established that for effluents of the Buzky canning plant, optimal use is made for purification of effluents of hypochlorite in the ratio of 0.5 dm3/m3 of wastewater that is being purified. To ensure environmental safety from possible contamination of unreacted hypochlorite of treated wastewater, an estimation of the cost of hyprochlorite for purification and comparison of residual concentrations with normalized parameters was carried out. The kinetics of oxidation of organic impurities in effluents was also studied, which was expressed by the degree of reduction of the COC solution in the process of reacting it with sodium hypochlorite. It is proved that the kinetics of the process of oxidation of organic contaminants is described by the equation valid for monomolecular reactions. For the system under study, the rate constant of the oxidation process of organic contaminants is 0,074 1/s. The principal scheme of purification of the effluents of the Buzky canning plant by sodium hypochlorite is proposed.

Determination of pyrrolizidine alkaloids in dietary sources using a spectrophotometric method

Pyrrolizidine alkaloids (PAs) are a class of toxic compounds found in the composition of more than 6000 plants. People can be exposed to PAs by consuming phytotherapeutic products, food from crops contaminated with seeds of some species with high content of PAs, and/ or contaminated animal products like bee products. For this reason we developed and validated a method for quantitative determination of PAs, from the most frequently contaminated food sources, honey and flour. Colorimetric Ehrlich reagent method was used with standard addition (1mg/kg senecionine). The extraction solvent was methanol 50% acidified with citric acid to pH 2-3, as this solvent can be used for alkaloids and N-oxides. We found that, in extracting the alkaloid only once from the dietary sources, the percent of recovery is low (52.5% for honey, and 45.75% for flour). Using successive extractions, three times with the same solvent, the senecionine retrieval percentage increased to 86.0% for honey and 76.0% for flour. The method was validated using the following parameters: selectivity, linearity (0,25- 20 mg/ mL senecionine), accuracy (average recovery 93.5 - 107.93%) and precision (RSD 3,26-4.55%.). The calculated limit of quantification (0.174 mg/ mL) makes this method applicable for determining Pas occurring at toxic levels for consumers.

Phytochemical screening, antioxidant and antimicrobial activities of Prunella vulgaris for oral thrush

Plant imitative products have been used for medicinal purposes for centuries. In traditional Indian medicine or Ayurveda, Prunella Vulgaris and many other herbs have been used as medicine. Traditional uses of plants have led to investigating their bioactive compounds, which have resulted in the detection of a significant number of therapeutic properties. The aim of present investigation was carried out to evaluate the phytochemical, antioxidant and antimicrobial activity of chloroform and hydroalcoholic leaves extracts of Prunella Vulgaris against microbial strains causing oral infections. Both chloroform and hydroalcoholic extracts revealed the presence of carbohydrate, triterpenoids/ steroids, flavonoids, tannin, phenolic compound and saponins were absent in only the chloroform extract. The bioactivities of the leaf extracts were qualified to their phytochemical constituents. Quantitative analysis of phenolic and flavonoids was carried out by Folins Ciocalteau reagent method and aluminium chloride method respectively. The In vitro antioxidant activity of chloroform and hydroalcoholic leaves extracts of Prunella Vulgaris was assessed against 2,2-diphenyl-1-picryl- hydrazyl (DPPH) radical scavenging activity, reducing power assay using standard protocols. The antimicrobial activity of chloroform and hydroalcoholic extracts of medicinal plants was evaluated using well diffusion method against Escherichia coli and Candida albicans. The TPC in chloroform extract was higher than that of the hydroalcoholic extract with concentration being 0.443 mg/g equivalent to gallic acid. The TFC in hydroalcohoilc extract was higher than that of the chloroform extract with concentration being 0.358 mg/g equivalent to rutin. The present study recognized leaves extract of Prunella vulgaris as a promising antioxidant and antimicrobial agent. However, further investigations are needed to understand the mechanistic basis of this effect of the extract and its chemical constituents thereof.
 Keywords: Prunella vulgaris, Phytochemical, Antioxidant, Antimicrobial activity, Folins Ciocalteau reagent, Quantitative analysis

Chemical characterisation and antioxidant activity of Aphandra natalia mesocarp and its oil from the Amazon region of Ecuador

The Aphandra natalia palm is a species of monotypic genus Aphandra belonging to the Arecaceae family and is native to South America, mainly in the western part of the Amazon basin in Ecuador, Peru, and Brazil. In this study, we determined the physicochemical characteristics of A. natalia fruit mesocarp, as well as its fatty acid composition, the total polyphenol content (TPC), lipophilic antioxidant compounds and total antioxidant capacity. The fatty acids profile was determined using GC–MS analysis and TPC and antioxidant activity by Folin Ciocalteu’s reagent method and by radical scavenging activity (1,1-diphenyl-2-picrylhydrazyl free radical [DPPH]), respectively regarding their oil. A high value of 57.92% of total lipids was obtained, thus it can be considered as new source of vegetable oil. The oil extracted from A. natalia mesocarp had a high oleic acid content (71.92%), which is a characteristic closer to the composition of olive oil than traditional palm oil. TPC value (83.57 ± 1.84 mg GAE kg−1 of oil) obtained from A. natalia fruit pulp oil is similar to some olive oil varieties. However, its oil presents smaller antioxidant activity than olive oils usually have.

Alteration of Bioactive Compounds in Two Varieties of Basil (Ocimum basilicum) Grown Under Different Light Spectra

Common basil is well known for its aromatic and medicinal properties. This study was aimed to investigate effects of light spectrum on essential oil composition, antiradical activity and total phenolic and anthocyanin contents of green and purple varieties of Ocimum basilicum. Plants were placed under different spectral light compositions including red (R), blue (B), white (W), 50 % red+50 % blue (50:50 % R:B) and 70 % red+30 % blue (70:30 % R:B) provided by LED modules with same light intensity for a duration of 30 days. Antiradical activity, total phenolic and anthocyanin contents and essential oil composition were measured using the DPPH radical scavenging assay, Folin-Ciocalteu reagent, spectrophotometric and GC-MS methods, respectively. Growing both basil varieties under 70:30 % R:B light resulted in higher antiradical activity than that of other light spectra. Highest amount of total phenolic and anthocyanin contents were observed in leaves of plants grown under 70:30 % R:B light in green and purple varieties, respectively. GC-MS analysis showed that essential oil compounds in both basil varieties were influenced by spectral light composition. In green basil, chemical composition of essential oil was improved by growing them under 70:30 % R:B light, while in purple variety R light induced production of limonene, α-pinene and β-myrcene. Close correlations were found between some essential oil compounds in plants grown under different light spectra. The effects of light spectra on bioactive compounds were depended on variety and type of compound. Therefore, through exposure to specific light spectrum it is possible to alter bioactive compound composition in basil plants.

PO-347 sONE, a novel tumour suppressor lncRNA, with diminished expression level in young triple negative breast cancer (TNBC) patients with lymphnode metastasis and large tumour size

IntroductionA recent surveillance of the human transcriptome has highlighted a pivotal class of RNA known as long non-coding RNA (lncRNAs). Myriad studies have reported the vital role of lncRNAs in oncology. However, the field is still in its infancy. lncRNAs are expressed in a highly cancer type-specific manner, thus could hold a lot of promises for personalised cancer therapy. In 2015, sONE has been identified as a novel lncRNA that acts as a post-transcriptional regulator of endothelial nitric oxide synthase (NOS3) expression in cultured endothelial cells. However, the role of sONE has never been investigated in any type of malignancy including Breast Cancer (BC). Among BC patients, TNBC is associated with the worst prognosis especially in young women (<40 years old). Young TNBC patients exhibit the most aggressive behaviour of tumours. Recently, few studies have been dedicated to identify actionable targets to this hard-to-treat group of patients. Therefore, our aim is to evaluate the expression pattern of sONE and its down-stream target (NOS3) in TNBC patients and to validate the role of sONE/NOS3/NO signalling cascade in TNBC cell linesMaterial and methodsBreast tissues were collected from 70 BC patients. Ki67 levels were quantified using immunohistochemistry. Clinical data of all patients were collected. MDA-MB-231 human BC cell lines were transfected using different oligonucleotides. Total RNA was extracted and quantified using qRT-PCR. NO production was assessed using Greiss reagent method. Cellular viability and proliferation were measured using MTT, BrdU assays, respectivelyResults and discussions sONE was significantly down-regulated in TNBC patients compared to its normal counterparts. Within TNBC patients, young females showed a diminished expression level of sONE. Furthermore, sONE expression pattern was inversely correlated with tumour size and lymph node metastasis in young TNBC patients. Our results showed that NOS3 expression level was reciprocally up-regulated.On the molecular level, sONE siRNAs resulted in an increase in NOS3 levels and NO production in MDA-MB-231. On the functional level, knocking down of sONE levels resulted in a significant increase of cellular viability and cellular proliferation rates of MDA-MB-231ConclusionThis study highlights a novel prognostic value of sONE in young TNBC patients where sONE expression level was negatively correlated with the aggressiveness of the disease. Moreover, this study validated sONE/NOS3/NO as a novel tumour suppressor signalling axis in BC patients.