Reaction Products Research Articles

The potential meat flavoring derived from Maillard reaction products of rice protein isolate hydrolysate-xylose via the regulation of temperature and cysteine

Maillard reaction products (MRPs) derived from rice protein isolate hydrolysate and D-xylose, with or without L-cysteine, were developed as a potential meat flavoring. The combined impact of temperature (80–140 °C) and cysteine on fundamental physicochemical characteristics, antioxidant activity, and flavor of MRPs were investigated through assessments of pH, color, UV–visible spectra, fluorescence spectra, free amino acids, volatile compounds, E-nose, E-tongue, and sensory evaluation. Results suggested that increasing temperature would reduce pH, deepen color, promote volatile compounds formation, and reduce the overall umami and bitterness. Cysteine addition contributed to the color inhibition, enhancement of DPPH radical-scavenging activity and reducing power, improvement in mouthfulness and continuity, reduction of bitterness, and the formation of sulfur compounds responsible for meaty flavor. Overall, MRPs prepared at 120 °C with cysteine addition could be utilized as a potential meat flavoring with the highest antioxidant activity and relatively high mouthfulness, continuity, umami, meaty aroma, and relatively low bitterness.

Accelerating PROTACs Discovery Through a Direct-to-Biology Platform Enabled by Modular Photoclick Chemistry.

Proteolysis targeting chimeras (PROTACs) have emerged as a promising strategy for drug discovery and exploring protein functions, offering a revolutionary therapeutic modality. Currently, the predominant approach to PROTACs discovery mainly relies on an empirical design-synthesis-evaluation process involving numerous cycles of labor-intensive synthesis-purification and bioassay data collection. Therefore, the development of innovative methods to expedite PROTAC synthesis and exploration of chemical space remains highly desired. Here, a direct-to-biology strategy is reported to streamline the synthesis of PROTAC libraries on plates, enabling the seamless transfer of reaction products to cell-based bioassays without the need for additional purification. By integrating amide coupling and light-induced primary amines and o-nitrobenzyl alcohols cyclization (PANAC) photoclick chemistry into a plate-based synthetic process, this strategy produces PROTAC libraries with high efficiency and structural diversity. Moreover, by employing this platform for PROTACs screening, wesmoothly found potent PROTACseffectively inhibit triple-negative breast cancer (TNBC) cell growth and induce rapid, selective targeted degradation of cyclin-dependent kinase 9 (CDK9). The study introduces a versatile platform for assembling PROTACs on plates, followed by direct biological evaluation. This approach provides a promising opportunity for high-throughput synthesis of PROTAC libraries, thereby enhancing the efficiency of exploring chemical space and accelerating the discovery of PROTACs.

Development and Physicochemical Properties of New Cutting Fluid for Titanium Alloy Machining

This article focuses on titanium alloy research, using triethanolamine oleate as the lubricating additive, the reaction product of triethanolamine, monoethanolamine and decanoic acid as a rust inhibitor, together with modified organosilicon antifoam agent and triazine fungicide, a new type of titanium alloy cutting fluid with excellent environmental protection and rust inhibition was prepared, and its physicochemical properties were systematically investigated through a large number of experiments. The results show that the prepared cutting fluid meets all specifications and demonstrates promising application prospects.

Characterization of a unique pH-dependent amylosucrase from Deinococcus cellulosilyticus

The amylosucrase (ASase, EC 2.4.1.4) utilizes sucrose as the sole substrate to catalyze multifunctional reactions. It can naturally synthesize α-1,4-linked glucans such as amylose as well as sucrose isomers with more favorable properties than sucrose with a lower intestinal digestibility and non-cariogenic properties. The amino acid sequence of the asase gene from Deinococcus cellulosilyticus (DceAS) exhibits low homology with those of other ASases from other Deinococcus species. In this study, we cloned and expressed DceAS and demonstrated its high activity at pH 6 and pH 8 and maintained stability. It showed higher polymerization activity at pH 6 than at pH 8, but similar isomerization activity and produced more turanose and trehalulose at pH 6 than at pH 8 and produced more isomaltulose at pH 8. Furthermore, the molecular weight of DceAS was 226.6 kDa at pH 6 and 145.5 kDa at pH 8, indicating that it existed as a trimer and dimer, respectively under those conditions. Additionally, circular dichroism spectra showed that the DceAS secondary structure was different at pH 6 and pH 8. These differences in reaction products at different pHs can be harnessed to naturally produce sucrose alternatives that are more beneficial to human health.

Evolution of the nuclear spin-orbit splitting explored via the 32Si(d,p)33Si reaction using SOLARIS

The spin-orbit splitting between neutron 1p orbitals at 33Si has been deduced using the single-neutron-adding (d,p) reaction in inverse kinematics with a beam of 32Si, a long-lived radioisotope. Reaction products were analyzed by the newly implemented SOLARIS spectrometer at the reaccelerated-beam facility at the National Superconducting Cyclotron Laboratory. The measurements show reasonable agreement with shell-model calculations that incorporate modern cross-shell interactions, but they contradict the prediction of proton density depletion based on relativistic mean-field theory. The evolution of the neutron 1p-shell orbitals is systematically studied using the present and existing data in the isotonic chains of N=17, 19, and 21. In each case, a smooth decrease in the separation of the 1p3/2-1p1/2 orbitals is seen as the respective p-orbitals approach zero binding, suggesting that the finite nuclear potential strongly influences the evolution of nuclear structure in this region.

Bioinspired All‐Polyester Diblock Copolymers Made from Poly(Pentadecalactone) and Poly(3,4‐Ethylene Furanoate): Synthesis and Polymer Film Properties

AbstractBio‐based, fully degradable aliphatic‐aromatic block copolymers are synthesized from ω‐pentadecalatone and cyclic oligo(3,4‐ethylene furanoate). In the first approach, the ring‐opening polymerization of the cyclic oligo(3,4‐ethylene furanoate) is initiated by a poly(pentadecalactone) macroinitiator with a terminal hydroxy group. The reaction temperatures of the melt polymerization are 210–230 °C due to the high melting points of the oligo(3,4‐ethylene furanoate). Under these conditions, transesterification is observed. The blockiness of the reaction products depends on the reaction temperature and on the ratio of pentadecalactone to 3,4‐ethylene furanoate repeat units, which is 50:50, 80:20, and 90:10. At lower temperatures and more pentadecalactone content, the blockiness is larger. The number average molar mass of the block copolymers remains smaller than 20 000 g mol−1. In the second approach, poly(pentadecalactone) is functionalized with an alkyne group, and the OH group of the oligo(3,4‐ethylene furanoate) (molar mass 1900 g mol−1) is converted into an azide group. Connecting the two polymers in a copper‐catalyzed 1,3‐dipolar addition reaction (“click reaction”) yields block copolymers with a number average molar mass of 12 400 g mol−1. The mechanical properties of the polymer films are intermediate between those of the parent homopolymers.

Encapsulation of probiotic Lacticaseibacillus casei with whey protein isolate/soybean hull polysaccharide enhances cell viability in harsh conditions

Whey protein isolate (WPI)-soybean hull polysaccharide (SHP) Maillard reaction conjugates were used to encapsulate Lacticaseibacillus casei and determine the effect of encapsulation on bacterial viability during freeze drying, simulated pasteurization simulated gastrointestinal digestion and storage. The WPI-SHP conjugates were characterized by degree of conjugation, size-exclusion chromatography, infrared and fluorescence spectroscopy and other analyses. The degree of conjugation, the formation of Maillard reaction products and the content of high molecular weight conjugates increased with reaction time. The emulsification capacity and glass transition temperature (Tg) of the WPI-SHP conjugates peaked after 4 days of reaction, at a Tg of 140.51 ± 2.76 °C, an emulsification activity index of 9.12 m2/g and emulsion stability index of 87.01%. WPI-SHP4d had the best protection effect of probiotics under freeze drying, simulated pasteurization and gastrointestinal digestion. These findings provide valuable insights into the selection of encapsulating agents to improve probiotic viability in functional foods.

Effect of Recycled Fine Aggregates on the Mechanical and Drying Shrinkage Properties of Alkali-Activated Recycled Concrete.

In this paper, the workability, mechanical, ion leaching, and drying shrinkage properties of alkali-activated concrete with recycled coarse and fine aggregates were studied, and the pore structure and micro-morphology of different alkali-activated recycled aggregate concretes (AARACs) were characterized by using the mercury intrusion method and scanning electron microscopy, respectively. The experimental results showed that with the increase in the replacement rate of the recycled fine aggregate (RFA), the flowability showed a decreasing trend. Adding a certain amount of RFA improves the mechanical properties of the AARAC. The compressive strength at a curing age of 28 days was 65.3 MPa with 70 wt% RFA replacement. When the replacement rate of the RFA was 100 wt%, the maximum splitting tensile strength (4.5 MPa) was obtained at a curing age of 7 days. However, the addition of the RFA had little effect on the flexural strength of the AARAC. As an extension of the curing age, the splitting tensile strength, flexural strength, tension-to-compression ratio, and flexure-to-compression ratio all showed an increasing trend at first and then a decreasing trend. At a curing age of 7 days, the tension-to-compression ratio and flexure-to-compression ratio were both high (except for those of R100), indicating that the ductility and toughness of the specimen were improved. The addition of the RFA increased the drying shrinkage of the AARAC. At a curing age of 120 days, compared to the specimen without the RFA, the drying shrinkage rate of the specimen with the addition of 70 wt% RFA increased by 34.15%. As the curing age increased, the microstructure of the reaction products became denser, but the proportion of large-diameter pores increased. This study evaluated the application of RFA in AARAC. The experimental results showed that the RFA-based AARAC had acceptable mechanical and durability properties.

Spatial Distribution and Seasonal Variation of Antibiotic-Resistant Bacteria in an Urban River in Northeast China

As the largest freshwater river flowing through Harbin, the Songhua River is a standby water source. It is very important to know the species and distribution of antibiotic-resistant bacteria (ARB) in the river. In this study, five antibiotics were selected to screen and identify ARB in spring and autumn. The results showed that the concentration of cefotaxime-resistant bacteria was the highest, and the maximum concentration at S6 in spring was up to 1.40 × 104 CFU/mL. In spring and autumn, bacteria resistant to three antibiotics were screened at S1 of the Songhua River, and bacteria resistant to five antibiotics were screened at S6. No multiple antibiotic-resistant bacteria (MARB) were screened in the other four sites in autumn, while MARB were screened in the other three samples except S2 in spring. In all sample areas in spring and autumn, the probability of screening MARB at S1 and S6 was the highest, reaching 100%. The identification results of 16S rDNA polymerase chain reaction (PCR) products of ARB showed that a total of 51 ARB strains from 15 bacterial genera were screened in the Songhua River, of which 20 ARB strains were from Pseudomonas. Among the 15 bacterial genera, bacteria from 8 bacterial genera have pathogenicity. The results of this study revealed the concentration, spatial distribution, and seasonal variation of culturable ARB in the Songhua River, providing data support for the remediation of antibiotic resistance gene (ARG) pollution in the river.

A Comparison of Different Biomass Combustion Mechanisms in the Transient State

Different combustion reaction process models were used to numerically study the behavior of the temperature, velocity, and turbulence fields, as well as to gain a better understanding of the differences between the reaction products obtained with each model. Transient-state simulations were conducted for a gasifier under specific operating conditions. The standard K-epsilon (2eq) turbulence model was utilized, along with the incorporation of species transport, volumetric responses, and eddy dissipation. In this study, the impacts of one-, two-, and four-step reaction mechanisms on the mass fraction of the products of the reactions, as well as the maximum values of velocity, turbulence, and temperature, were examined. The findings demonstrated that for all mechanisms, the greater maximum values of velocity and turbulence are attained at early time steps and decrease with subsequent time steps. The temperature rises as much in the early time steps and nearly stays the same in the late time steps. In all situations examined, the species’ fraction mass varies slightly in the early time steps but becomes nearly constant in the latter time stages. Similar species mass fraction values were found for both one-step and four-step methods. The results also suggest that the lower half of the gasifier is where the highest mass fraction values are found.

Effect of Public Fillers on Cement-Stabilized Recycled Mixes of Road Performance: Mechanical Properties, Microstructure, and Durability.

In order to address the challenges of resource utilization posed by construction waste, the substitution of natural aggregate (NA) with public fill (PF) contents was investigated for load reclamation and road grassroots applications. A comprehensive assessment of road performance for the recycled mixture was conducted, focusing on parameters such as unconfined compressive strength, splitting strength, compressive resilience modulus, dry shrinkage, and frost resistance. Additionally, the impact of incorporating PF at various types and replacement ratios on the microstructure of cement-stabilized aggregate (CSA) was analyzed. The results indicated that the unconfined compressive strength of cement-stabilized recycled mixture with varying PF contents meets the base strength requirements for heavy, medium, and light traffic pavement on secondary and sub-secondary roads in China. Notably, the unconfined compressive strength and resilience modulus follow a similar pattern, reaching their peak at a 25% PF content. Microscopic examination reveals that an appropriate PF content leads to the predominant formation of C(N)-A-S-H, hydrotalcite, Ca(OH)2, and CaCO3 as paste reaction products. As the replacement of public fill increases from 0% to 25%, there is a gradual stacking of gel products, which enhances the compactness of the microstructure by cementing together unreacted particles. Consequently, this process reduces dry shrinkage strain and effectively mitigates the formation of reflection cracks. Applying large quantities of public fill to road construction can effectively deal with various waste accumulation problems and produce a novel road material with significant social, economic, and environmental benefits.

Reactive Eutectic Media for Lignocellulosic Biomass Fractionation.

This study introduces an alternative approach towards lignocellulosic biomass fractionation. For this purpose, reactive eutectic media (REM) based on ammonium formate and different organic acids are investigated, possible products are identified, and the REM are employed for lignin extraction and terminal isolation of cellulose pulp from beech wood. The method promises a considerable process intensification by simultaneous separation of high purity cellulose pulp, lignin isolation as a cationically modified species, and production of value-added chemicals from reaction products of the REM. This study puts a further focus on the generated cellulose pulp and investigates it with respect to surface charge and fiber length.

Maillard Reaction-Derived S-Doped Carbon Dots Promotes Downregulation of PPARγ, C/EBPα, and SREBP-1 Genes In-Vitro.

Carbon nanodots (CDs) are commonly found in food products and have attracted significant attention from food scientists. There is a high probability of CD exposure in humans, but its impacts on health are unclear. Therefore, health effects associated with CD consumption should be investigated. In this study, we attempted to create a model system of the Maillard reaction between cystine and glucose using a simple cooking approach. The CDs (CG-CDs) were isolated from cystine-glucose-based Maillard reaction products and characterized using fluorescence spectroscopy, X-ray diffractometer (XRD), and transmission electron microscope (TEM). Furthermore, human mesenchymal stem cells (hMCs) were used as a model to unravel the CDs' cytotoxic properties. The physiochemical assessment revealed that CG-CDs emit excitation-dependent fluorescence and possess a circular shape with sizes ranging from 2 to 13 nm. CG-CDs are predominantly composed of carbon, oxygen, and sulfur. The results of the cytotoxicity evaluation indicate good biocompatibility, where no severe toxicity was observed in hMCs up to 400 μg/mL. The DPPH assay demonstrated that CDs exert potent antioxidant abilities. The qPCR analysis revealed that CDs promote the downregulation of the key regulatory genes, PPARγ, C/EBPα, SREBP-1, and HMGCR, coupled with the upregulation of anti-inflammatory genes. Our findings suggested that, along with their excellent biocompatibility, CG-CDs may offer positive health outcomes by modulating critical genes involved in lipogenesis, homeostasis, and obesity pathogenesis.

Toxicity of vapor-gas flows from technogenic substance

The relevance. Vapor-gas flows from technogenic substances (storage wastes of the mining industry), carrying a high amount of potentially toxic elements, are a big problem for the environment and the population, because metals and metalloids in the composition of emanations are in an easily digestible bioavailable forms and are able to easily penetrate into living organisms. The main aim of this study is an assessment of toxicity for living organisms of air mixtures containing gaseous products emitted by a surfaces of mine tailings freely located near settlements and under the influence of climatic factors and microorganisms. Methods. Carrying out experiments with white rats male Wistar, analysis of organs for the content of a wide range of elements by ICP-MS on a NexION 300D mass spectrometer (PerkinElmer, USA) (PNL GGC IShPR TPU). In the experiment, a pooled sample of the waste substance was used. The sample was analyzed for the content of oxides of the silicate group by XRF using X-ray fluorescence spectrometer ARL-9900-XP (Thermo Electron Corporation, Switzerland) at the Laboratory of X-ray Spectral Analysis Methods of the IGM SB RAS; elemental analysis was carried out by ICP-MS using an ELAN-9000 DRC-e instrument Perkin Elmer, USA (HAC Plasma). Results. Under the influence of vapor-gas emanations from the substance of arsenide tailings of the Tuvacobalt plant on a group of rats male Wistar, pathological changes occurred in the experimental group. Small foci of hemorrhages formed in the lungs, the tissues became edematous and inflamed. The liver of all animals increased, the share structure became sharply expressed, the color became light beige. Single hemorrhages were found in the kidneys. The vessels of the meninges are dilated, petechial hemorrhages are observed. The synthetic function of cells and the stability of cellular and intracellular membranes were disrupted, first of all, the endothelium of blood vessels was affected, the physiological functioning of organs and tissues was disrupted with the accumulation of intermediate products of biochemical reactions, determined macroscopically. Also, dystrophy of internal organs was determined, in particular, the liver, lungs, kidneys, and brain. Elemental analysis of animal organs showed the accumulation of toxic elements in comparison with the control group. The most characteristic elements, the excess of which was recorded in most cases, are mercury and arsenic. They were unevenly distributed throughout the organs. Mercury accumulated mainly in the lungs, kidneys and liver, while arsenic accumulated mainly in the liver and brain. This may indicate the entry of mercury and arsenic ions in a biologically available form into the bloodstream by inhalation, and local irritating and toxic effects on the lungs were not prevailing, and the liver was the most damaged organ according to the results of toxicochemical and morphological studies. The homeostasis of micro and macro elements is finely regulated, and any concentration imbalance in the form of a deficiency leads to a progressive decrease and disruption of normal biological function, to severe physiological and clinical outcomes, which we observed from the results of the experiment. The data obtained proved the acute toxic effect of vapor-gas flows on living organisms, and also confirmed the possibility of the removal of many chemical elements in easily accessible and assimilable forms.

Detection of electrocatalytical and -chemical processes by means of in situ flow NMR spectroscopy

In situ studies of electrochemical processes using NMR offer valuable information on reaction mechanisms, kinetics, and species identification, making it a powerful tool in electrochemistry research. In this study, we present the design of an in situ redox-flow NMR cell that allows for a continuous flow of liquid (electrolyte) or gas, application of electrical voltage, and recording of NMR signals. The utility of this setup is demonstrated through two case studies: electrochemical copper deposition on a gold electrode and the electrochemical conversion of carbon dioxide into hydrocarbon products. Specifically, the presence of multicarbon products containing C–C bonds generated during the electrochemical reduction reaction is confirmed in the 2H NMR spectra in the latter example. These findings highlight the ability of the in situ redox-flow NMR cell to directly monitor reaction intermediates and products, thereby enabling the elucidation of reaction mechanisms for the efficient and selective production of valuable hydrocarbon products through the conversion of CO2 into value-added chemicals. In contrast to other reported in situ NMR cells, the presented cell is suitable for multiple uses, and allows detecting NMR signals not only from exhaust products but also from those formed on the catalyst surface.

Effects of Steel Slag on the Hydration Process of Solid Waste-Based Cementitious Materials.

Aiming to enhance the comprehensive utilization of steel slag (SS), a solid waste-based binder consisting of SS, granulated blast furnace slag (BFS), and desulfurization gypsum (DG) was designed and prepared. This study investigated the reaction kinetics, phase assemblages, and microstructures of the prepared solid waste-based cementitious materials with various contents of SS through hydration heat, XRD, FT-IR, SEM, TG-DSC, and MIP methods. The synergistic reaction mechanism between SS and the other two wastes (BFS and DG) is revealed. The results show that increasing SS content in the solid waste-based binder raises the pH value of the freshly prepared pastes, advances the main hydration reaction, and shortens the setting time. With the optimal SS content of 20%, the best mechanical properties are achieved, with compressive strengths of 19.2 MPa at 3 d and 58.4 MPa at 28 d, respectively. However, as the SS content continues to increase beyond 20%, the hydration process of the prepared binder is delayed. The synergistic activation effects between SS and BFS with DG enable a large amount of ettringite (AFt) formation, guaranteeing early strength development. As the reaction progresses, more reaction products CSH and Aft are precipitated. They are interlacing and overlapping, jointly refining and densifying the material's microstructure and contributing to the long-term strength gain. This study provides a reference for designing and developing solid waste-based binders and deepens the insightful understanding of the hydration mechanism of the solid waste-based binder.

The use of δ13C in CO to determine removal of CH4 by Cl radicals in the atmosphere

Abstract The reaction of CH4 with chlorine (Cl) radicals in the atmosphere is associated with an extraordinarily strong isotopic fractionation, where 12CH4 reacts about 70 ‰ faster with Cl than 13CH4. Therefore, although the Cl-based sink of CH¬4 constitutes only a small contribution to its total removal rate, the uncertainty in this small sink has been identified as one of the two largest uncertainties of isotope-based CH4 source apportionment at the global scale. The uncertainty arises from the fact that Cl levels in the atmosphere are so low that they cannot be detected directly. One very sensitive indirect method to identify and quantify the CH4 + Cl reaction in the atmosphere is the detection of the extremely 13C-depleted reaction product carbon monoxide (CO) from this reaction. This article reviews the concept of this approach, its successful application in the atmosphere, its challenges and opportunities for identifying and quantifying Cl-based removal of CH4 at the regional and global scale and its potential to detect and evaluate possible attempts to enhance CH4 removal from the atmosphere.

Mechanism of Interfacial Molecular Interactions Reveals the Intrinsic Factors for the Highly Enhanced Sensing Performance of Ag-Loaded Co3O4.

The noble metal-loaded strategy can effectively improve the gas-sensing performances of metal oxide sensors. However, the gas-solid interfacial interactions between noble metal-loaded sensing materials and gaseous species remain unclear, posing a significant challenge in correlating the physical and chemical processes during gas sensing. In this study, in situ diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) and in situ Raman spectroscopy were conducted to collaboratively investigate the interfacial interactions involved in the ethanol gas-sensing processes over Co3O4 and Ag-loaded Co3O4 sensors. In situ DRIFTS revealed differences in the compositions and quantities of sensing reaction products, as well as in the adsorption-desorption interactions of surface species, among Co3O4 and Ag-loaded Co3O4 materials. In parallel, in situ Raman spectroscopy demonstrated that the ethanol atmosphere can modulate the electron scattering of Ag-loaded Co3O4 materials but not of raw Co3O4. In situ experimental results revealed the intrinsic reason for the highly enhanced sensing performances of the Ag-loaded Co3O4 sensors toward ethanol gas, including a decreased optimal working temperature (from 250 to 150 °C), an improved gas response level (from 24 to 257), and accelerated gas recovery dynamics. This work provides an effective platform to investigate the interfacial interactions of sensing processes at the molecular level and further advances the development of high-performance gas sensors.

Behavior of Lithium Amide Under Argon Plasma.

Alkali and alkaline earth metal amides are a type of functional materials for hydrogen storage, thermal energy storage, ion conduction, and chemical transformations such as ammonia synthesis and decomposition. The thermal chemistry of lithium amide (LiNH2), as a simple but representative alkali or alkaline earth metal amide, has been well studied previously encouraged by its potentials in hydrogen storage. In comparison, little is known about the interaction of plasma and LiNH2. Herein, we report that the plasma treatment of LiNH2 in an Ar flow under ambient temperature and pressure gives rise to distinctly different reaction products and reaction pathway from that of the thermal process. We found that plasma treatment of LiNH2 leads to the formation of Li colloids, N2, and H2 as observed by UV-vis absorption, EPR, and gas products analysis. Inspired by this very unique interaction between plasma and LiNH2, a chemical loop for ammonia decomposition to N2 and H2 mediated by LiNH2 was proposed and demonstrated.

CCT6A regulates cervical cancer cell glycolysis and proliferation under hypoxic conditions via the TCAB1/TERT.

Cervical cancer is one of the most common female malignancies worldwide, a hypoxic microenvironment usually causes enhanced viability and glycolytic capacity of cervical cancer cells. The aim of this study was to investigate the association between chaperonin containing t-complex polypeptide 1 subunit 6A (CCT6A) and cell proliferation as well as hypoxic glycolysis. Bioinformatics analysis was conducted to explore the association between cervical cancer and its partner protein under hypoxic conditions, namely CCT6A. Subsequently, the expression of CCT6A was silenced, and the effects of CCT6A silencing on cervical cancer cell proliferation, cell cycle, glycolysis-related proteins, and telomerase activity were examined. Bioinformatics analysis was performed to investigate the expression of CCT6A in cervical cancer under hypoxic conditions. The expression of CCT6A was silenced in cervical cancer cells, Hela and Siha, to study its effects on cell proliferation and hypoxic glycolysis. The localization of telomerase activity-related proteins, TCAB1 and TERT, was detected using immunofluorescence, and their interaction was assessed using immunoprecipitation. A cellular hypoxia model was established, and the products of the glycolysis reaction were detected. A nude mouse tumor model was constructed, and the changes in glycolysis-related proteins in tumor tissues were examined using western blot, while Ki67 expression in tumor tissues was evaluated by immunohistochemistry. Telomerase activity was found to be enhanced in CCT6A-silenced cervical cancer cells, along with an increase in telomerase cajal body protein 1 (TCAB1) and telomerase reverse tranase (TERT) protein binding associated with telomerase activity. Additionally, the proportion of cells in the Gap 2/mitosis (G2/M) stage and the 5-ethynyl-2'-deoxyuridine (EdU) positivity rate were decreased in CCT6A-silenced cells, indicating a reduction in cell proliferation. The expression of cell cycle-related proteins, including Cyclin E, CCNA2 and cyclin-dependent kinase 2 (CDK2), was suppressed. Furthermore, under a hypoxic environment, silencing CCT6A led to a significant reduction in cell viability and downregulation of glycolysis-related proteins, such as lactate dehydrogenase A (LDHA) and hexokinase 2 (HK2). Mechanistically, silencing CCT6A may reduce telomerase activity by inhibiting the TCAB1/TERT interaction. Additionally, TERT was found to activate the promoter region of the HK2 gene, and inhibition of TERT activity reduced the transcriptional level of HK2. The study primarily explored the involvement of CCT6A in cervical cancer, yet it did not account for the myriad of other elements potentially influencing cell proliferation and glycolysis. It's essential to recognize that cervical cancer's etiology is multifaceted, shaped by an array of genetic variations, environmental influences, and protein interactions. Silencing CCT6A could effectively attenuate the upregulation of cell proliferation and glycolytic function mediated by TCAB1/TERT in cervical cancer cells.