Reactions Of Nucleic Acids Research Articles

Differential Staining of Nucleic Acids

1. Thionin, being a basic dye of thiazine group, stains DNA and PNA of the cell differentially in blue (α) and purple (β) respectively.2. Metachromasia of thionin ' can be observed in vitro by mixing solution of the dye with various amounts of nucleic acids of whatever type. When large excess of nucleic. acids is added with small amount of thionin, the color of the solution is blue (α); increase in the amount of the dye in comparison with that of nucleic acids causes gradual change of the color taken by the solution from blue to purple (β) and finally precipitate in the latter coloration appears.3. Marked metachromasia caused by nucleic acids in vitro is shared by toluidine blue and cresyl violet, but metachromatic staining of nucleic acids in situ with these dyes can not so readily observed as with thionin., 4. Either of the α- and βcolors taken by these dyes can readily be distinguished from the metachromasia caused by mucopolysaccharides (γ-color).5. Staining of nucleic acids with toluidine blue is partially inhibited by the presence of sodium chloride in high concentration. Thionin-nucleic acid complex can be dissociated by saturated sodium chloride solution in vitro as well as in situ. These facts suggest that nucleic acid stainings with thionin and toluidine blue are of electrostatic nature.6. Partial inhibition of the staining reaction of nucleic acids with thionin, toluidine blue, or cresyl violet by different means gives rise to PNA stained in a-color, which otherwise stains in β-color.7. On the basis of an experiment using histone and DNA, the a-color of thionin taken by cellular DNA is assumed to be resulted from competition of proteins for binding DNA. No such evidence was ob-tained with cellular PNA.8. Implications of the metachromasia of some basic dyes caused by nucleic acids and of the ints ference of proteins in nucleic acid staining with basic dyes are discussed.Acknowledgements. It is a pleasure to acknowledge my indebtedness to Dr. O. Itikawa for his encouragement and to late Miss. H. Kawata, Miss S. Takeda, Mr. T. E. Yukimura and Mr. O. Harikane for their assistance.

Cytochemical reactions of nucleic acids.

Cytochemical reactions of nucleic acids.

The reaction of nucleic acids with mustard gas

Research Article| January 01 1948 The reaction of nucleic acids with mustard gas D. T. Elmore; D. T. Elmore 1Chemistry Department, University College, Nottingham Search for other works by this author on: This Site PubMed Google Scholar J. M. Gulland; J. M. Gulland 1Chemistry Department, University College, Nottingham Search for other works by this author on: This Site PubMed Google Scholar D. O. Jordan; D. O. Jordan 1Chemistry Department, University College, Nottingham Search for other works by this author on: This Site PubMed Google Scholar H. F. W. Taylor H. F. W. Taylor 1Chemistry Department, University College, Nottingham Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1948) 42 (2): 308–316. https://doi.org/10.1042/bj0420308 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation D. T. Elmore, J. M. Gulland, D. O. Jordan, H. F. W. Taylor; The reaction of nucleic acids with mustard gas. Biochem J 1 January 1948; 42 (2): 308–316. doi: https://doi.org/10.1042/bj0420308 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1948 CAMBRIDGE UNIVERSITY PRESS1948 Article PDF first page preview Close Modal You do not currently have access to this content.

ALLERGY

<h3>GENERAL CONSIDERATIONS</h3> Cooke¹discussed the protein derivatives histamine, proteose and nucleic acid as factors in allergy. He concluded his discussion of histamine with this statement: "... with regard to clinical studies, my results are not in accord with most of those reported. I find no unresponsive phase in urticaria [it has been assumed that if a spontaneous urticarial wheal appears in a certain area, it will not reappear in that area for thirty-six to forty-eight hours], no increase of tolerance to histamine, no clinical result in chronic urticaria and no evidence that histamine is increased in the blood during general allergic reactions." Regarding proteose, he expressed the belief that clinical sensitization to derivatives of the digestion of protein, at least to those in the proteose state, existed. Regarding nucleic acid he stated that in his clinic certain patients sensitive to ragweed had been tested with nucleic acid and positive reactions

The Serological Reactivity of Nucleic Acid

Summary The reaction of nucleic acids with certain antibacterial sera has been studied and the following points made: Nucleic acids give a specific precipitate with certain antisera, in particular equine antipneumococcic sera.The reactive substance is in the euglobulin fraction of the serum.The reaction is sensitive to changes in ionic strength. Also for a given ionic strength there is a difference in the amount of precipitate obtained when different ions are present.The reaction is specifically inhibited under suitable conditions by purine nucleotides, purine nucleosides and purine bases. Pyrimidine bases show only weak inhibition and pentoses and phosphates do not inhibit the reaction at all.