Reaction Of H2O2 Research Articles

Monolayer Amphiphiles Hydrophobicize MoS2-Mediated Real-Time Water Removal for Efficient Waterproof Hydrogen Detection.

Ensuring water-fouling-free operation of semiconductor-based gas sensors is essential to maintaining their accuracy, reliability, and stability across diverse applications. Despite the use of hydrophobic strategies to prevent external water intrusion, addressing in situ-produced water transport during H2 detection remains a challenge. Herein, we construct a novel waterproof H2 sensor by integrating single-atom Ru(III) self-assembly with monolayer amphiphiles embedded in MoS2. The unique monolayer structure enables the sensor to detect H2 in the presence of water, as well as facilitate the self-transport of in situ-generated water from the H2-O2 reaction during H2 detection. Molecular dynamics simulations reveal that monolayer amphiphiles exhibit a higher water diffusion coefficient than multilayer amphiphiles, making them more advantageous for removing in situ-produced water. Deployable on mobile platforms, it enables wireless H2cat detection for up to 6 months, without the introduction of protective membranes against dust and water ingress. This work not only enhances the performance of H2 detection but also introduces a new concept for the advancement of stable water-sensitive sensors.

Redox proteomic analysis of H2O2 -treated Jurkat cells and effects of bicarbonate and knockout of peroxiredoxins 1 and 2

Oxidation of thiol proteins and redox signaling occurs in cells exposed to H2O2 but mechanisms are unclear. We used redox proteomics to seek evidence of oxidation of specific proteins either by a mechanism involving reaction of H2O2 with CO2/bicarbonate to give the more reactive peroxymonocarbonate, or via a relay involving peroxiredoxins (Prdxs). Changes in oxidation state of specific Cys-SH residues on treating Jurkat T lymphoma cells with H2O2 were measured by isotopically labeling reduced thiols and analysis by mass spectrometry. The effects of bicarbonate and of knocking out either Prdx1 or Prdx2 were examined. Approximately 14,000 Cys-peptides were detected, of which ∼ 1% underwent 2-10 fold loss in thiol content with H2O2. Those showing the most oxidation were not affected by the presence of bicarbonate or knockout of either Prdx. Consistent with previous evidence that bicarbonate potentiates inactivation of glyceraldehyde-3-phosphate dehydrogenase, the GAPDH active site Cys residues were significantly more sensitive to H2O2 when bicarbonate was present. Several other proteins were identified as promising candidates for further investigation. Although we identified some potential protein candidates for Prdx- dependent oxidation, most of the significant differences between KO and WT cells were seen in proteins for which H2O2 unexpectedly increased their CysSH content over untreated cells. We conclude that facilitation of protein oxidation by bicarbonate or Prdx-mediated relays is restricted to a small number of proteins and is insufficient to explain the majority of the oxidation of cell thiols that occurs in response to H2O2.

Effect of ultrasound assisted H2O2 degradation on longan polysaccharide: degradation kinetics, physicochemical properties and prebiotic activity

This study aimed to investigate the effect of ultrasound-assisted H2O2 (US/H2O2) reaction on degradation parameters and kinetics, physicochemical properties and prebiotic activity of longan polysaccharide (LP). Results showed that US/H2O2 had a synergistic effect on the degradation of LP, and its kinetic equation followed to the fist – order model. US/H2O2 degradation did not change the chemical and monosaccharide composition of LP but altered their ratio. Compared with LP, three degraded polysaccharides (DLPs) displayed lower molecular weight, particle size and viscosity, but higher solubility. SEM and AFM revealed that US/H2O2 degradation led to significant differences in the microstructure and solution conformation of LP. Moreover, LP and DLPs showed different proliferation effects on four lactobacilli and bifidobacteria strains, among which DLP-8 (degraded for 8 h) exhibited the strongest prebiotic activity. US/H2O2 could be effectively applied to the degradation of LP to improve its physicochemical properties and bioactivities.

Shattering the Water Window: Comprehensive Mapping of Faradaic Reactions on Bioelectronics Electrodes.

It is generally accepted that for safe use of neural interface electrodes, irreversible faradaic reactions should be avoided in favor of capacitive charge injection. However, in some cases, faradaic reactions can be desirable for controlling specific (electro)physiological outcomes or for biosensing purposes. This study aims to systematically map the basic faradaic reactions occurring at bioelectronic electrode interfaces. We analyze archetypical platinum-iridium (PtIr), the most commonly used electrode material in biomedical implants. By providing a detailed guide to these reactions and the factors that influence them, we offer a valuable resource for researchers seeking to suppress or exploit faradaic reactions in various electrode materials. We employed a combination of electrochemical techniques and direct quantification methods, including amperometric, potentiometric, and spectrophotometric assays, to measure O2, H2, pH, H2O2, Cl2/OCl-, and soluble platinum and iridium ions. We compared phosphate-buffered saline (PBS) with an unbuffered electrolyte and complex cell culture media containing proteins. Our results reveal that the "water window"─the potential range without significant water electrolysis─varies depending on the electrolyte used. In the culture medium that is rich with redox-active species, a window of potentials where no faradaic process occurs essentially does not exist. Under cathodic polarizations, significant pH increases (alkalization) were observed, while anodic water splitting competes with other processes in media, preventing prevalent acidification. We quantified the oxygen reduction reaction and accumulation of H2O2 as a byproduct. PtIr efficiently deoxygenates the electrolyte under low cathodic polarizations, generating local hypoxia. Under anodic polarizations, chloride oxidation competes with oxygen evolution, producing relatively high and cytotoxic concentrations of hypochlorite (OCl-) under certain conditions. These oxidative processes occur alongside PtIr dissolution through the formation of soluble salts. Our findings indicate that the conventional understanding of the water window is an oversimplification. Important faradaic reactions, such as oxygen reduction and chloride oxidation, occur within or near the edges of the water window. Furthermore, the definition of the water window significantly depends on the electrolyte composition, with PBS yielding different results compared with culture media.

PM2.5 episodes in northern Taiwan under southerly winds in late winter

When northern Taiwan is influenced locally, PM2.5 events occur in specific conditions. If it is also affected by long-range transport from mainland China in the northwest, the probability of these PM2.5 events increases. The current study applies WRF/CMAQ to study such case but focus on the local. From April 7 to 9, 2019, the Pacific high pressure extended westward, causing the prevailing weak southerly wind near Taiwan. The intensity of the Pacific high pressure weakened, and the wind speed near Taiwan also weakened. Therefore, the PM2.5 concentration in Taiwan increased. In northern Taiwan, the hourly PM2.5 concentration will inevitably increase to approximately 70 μg m−3. The ISAM of the CMAQ model demonstrates that local contributions to daily PM2.5 concentrations at the four representative monitoring stations in northern Taiwan are 3.5 to 16.2 μg m−3, greater than those from central, southern, and eastern Taiwan, from 1.8 to 6.7 μg m−3. We use the integrated process rate (IPR) and integrated reaction rate (IRR) of CMAQ to explore the formation mechanisms of PM2.5. The main causes of the increase in PM2.5 concentration are horizontal advection (HADV), aerosol processes (AERO), emissions (EMIS), and a small amount of cloud processes and aqueous chemistry (CLDS). The main source of NO3− is the reaction between OH and NO2 during the day and the reaction between N2O5 and water vapor at night. The gaseous HNO3 concentration reaches a peak near noon or soon after. The aerosol ANO3 concentration is high in the early morning. We also use the Sulfur Tracking Method (STM) of CMAQ to explore the sources of SO42−. Mainly it is from the local reaction of SO2 and H2O2. Finally, we apply AERO7 in CMAQ to explore the main sources of carbon components. The organic carbon (OC) concentration is much greater than the element carbon (EC) concentration, which indicates a strong local photochemical effect in northern Taiwan. OC mainly comes from low-volatility/semivolatile oxidized combustion OC, followed by low-volatility/semivolatile POA. Similar weather conditions occur in autumn, winter, and spring. When the weak southerly wind was around Taiwan, PM2.5 in northern Taiwan was noticed.

Multivalent Pt/Ti3C2Tx Nanocomposite-Based Immunochromatographic Sensor for Colorimetric/Temperature/Pressure Trimodal Detection.

Multimodal immunochromatographic sensors (ICSs) have acquired extensive attention since they not only provide reliable results by comparing the different output signals but also flexibly respond to various application environments. Herein, an ICS with triple signal outputs including colorimetry, temperature, and pressure was developed for sensitive detection of chlorothalonil. The multivalent Pt/Ti3C2Tx nanoparticles as signal tags were facilely synthesized by loading PtNPs onto single-layer Ti3C2Tx nanosheets with high surface area. The acquired Pt/Ti3C2TxNPs accelerated the rate-limiting step of the aerogenesis reaction of H2O2 for producing intensive pressure signals due to their significant catalase-mimic activity. Meanwhile, they showed desirable photothermal conversion efficiency in the near-infrared region for producing significant temperature signals. Furthermore, their deep color also allowed facile colorimetry by using the naked eye. Based on a competitive immunoassay, chlorothalonil was detected as a model analyte on this trimodal ICS platform. The detection limits for pressure, temperature, and colorimetric modes were 0.04, 0.09, and 5 ng mL-1, respectively. The recoveries for detecting chlorothalonil supplemented in Astragalus and Honeysuckle with pressure mode were 84.0-110% and 108-114%, respectively. Therefore, the ICS presented a portable, sensitive, accurate, and flexible multimodal strategy suitable for point-of-care testing.

Chemical effect of H2 on NH3 combustion in an O2 environment via molecular dynamics simulations

Due to the imperative to reduce carbon emissions, NH3-H2 mixed combustion has garnered significant attention. However, the knowledge of the reaction kinetics of NH3-H2-O2 mixtures at the molecular level remains limited. In this paper, the reactive force-field (ReaxFF) method was employed to study the microscale reaction kinetics of the NH3-H2-O2 mixture with varying hydrogen ratios (HR). The results of the microcanonical ensemble simulations indicated that the addition of H2 significantly increased the combustion performance of NH3. However, H2 did not change the two primary reaction paths of NH3 oxidation (NH3 → N2H2 → N2 and NH3 → NO → N2). The addition H2 not only led to a shift in the initiation reactions from NH3-O2 to H2-O2 reactions, but also notably enhanced the concentration of HO2 and OH radicals (e.g., O2 + H2 → HO2 + H), which accelerated the extraction of H from NxHy and subsequently facilitated the immediate formation of N2. Furthermore, the ignition delay decreased from 120 ps to 40 ps, and the activation energies decreased from 295.46 kJ/mol to 162.12 kJ/mol as HR increased from 0 to 0.3 under the equivalence ratio of 0.4. These key findings can guide the development of clean NH₃ combustion technology.

Catalytic selectivity in activating peroxymonocarbonate with a commercial MnO2 catalyst for water remediation

An in-situ formed peroxymonocarbonate (HCO4-) oxidation system from an equilibrium reaction of H2O2 and HCO3– was established for eliminating organic pollutants in water. With H2O2 as a co-existing oxidant in the system, a commercial available MnO2 was able to proceed selective catalytic activation of HCO4- to generate reactive oxygen species (ROS), such as ·OH and CO3·-. The MnO2 catalyst showed excellent catalytic ability, structural stability and recyclability in the HCO4--based oxidation system for removing tetracycline. The degradation rate of tetracycline reached 86 % and the catalyst could be used for at least 5 times with only 0.1 ppm of Mn leached in water. The kinetic study indicated that HCO4- was more related to the degradation of tetracycline and generated ·OH at the yield of 40 % was primarily from HCO4- activation rather than H2O2 activation. The evaluation of ROS and quenching experiments suggested that CO3·- was most responsible for tetracycline degradation. The catalyst characterization and reaction mechanism indicated that low-valent Mn species were the active sites for HCO4- activation. Finally, in-situ formation of HCO4- derived from CO2 in Na2CO3 solution with H2O2 was preliminarily explored, presenting a MnO2-catalzyed CO2-derived HCO4--based oxidation system for pollutant control. This study shows that a cheap and commercial available MnO2 can be an efficient, facile and selective catalyst for in-situ formed HCO4--based advanced oxidation processes.

A Schottky/Z-Scheme Hybrid for Augmented Photocatalytic H2 and H2O2 Production.

The prodigious employment of fossil fuels to conquer the global energy demand is becoming a dreadful threat to the human society. This predicament is appealing for a potent photocatalyst that can generate alternate energy sources via solar to chemical energy conversion. With this interest, we have fabricated a ternary heterostructure of Ti3C2 nanosheet modified g-C3N4/Bi2O3 (MCNRBO) Z-scheme photocatalyst through self-assembly process. The morphological analysis clearly evidenced the close interfacial interaction between g-C3N4 nanorod, Bi2O3 and Ti3C2 nanosheets. The oxygen vacancy created on Bi2O3 surface, as suggested by XPS and EPR analysis, supported the Z-scheme heterojunction formation between g-C3N4 nanorod and Bi2O3 nanosheets. The collaborative effect of Z-scheme and Schottky junction significantly reduced charge transfer resistance promoting separation efficiency of excitons as indicated from PL and EIS analysis. The potential of MCNRBO towards photocatalytic application was investigated by H2O2 and H2 evolution reaction. A superior photocatalytic H2O2 and H2 production rate for MCNRBO is observed, which are respectively around 5 and 18 folds higher as compared to pristine CNR nanorod. The present work encourages for the development of a noble, eco-benign and immensely efficient dual heterojunction based photocatalyst, which can acts as saviour of human society from energy crisis.

Performance and Mechanism of the Modified Group Regulated the MIL-101(Fe) Type Fenton-like Catalysts.

In order to avoid the disadvantages of the Fenton process in wastewater treatment and reduce the cost of wastewater treatment, a series of MIL-101(Fe)-X (X = -OH, -NH2, -NO2, -H) solid Fenton catalysts were successfully prepared. The performance of these Fenton-like catalysts was studied with the Fenton experiment as a reference and methylene blue (MB) as an organic pollutant. The effects of the H2O2 concentration, catalyst dosage, and reaction pH on catalytic performance were systematically studied. The research had shown that the optimal concentration of H2O2 for catalytic reactions was 0.10 mmol/L and the pH was 3. At this point, their catalytic degradation MB performance was superior to the Fenton reaction and photocatalytic reaction. When the H2O2 participated in the reaction, the performance of MIL-101(Fe)-X (X = -OH, -NH2, -NO2, -H) in catalyzing the degradation of MB followed the rule of -OH > -NH2 > -NO2 > -H. This was due to the synergistic effect of Fenton-like catalysis and photocatalytic degradation in the catalytic degradation of MB. In addition, the electron paramagnetic resonance and electrospray ionization mass spectrometry showed that the hydroxyl radical (·OH) generated during the catalytic process first underwent a redox reaction with the highly electronegative functional groups in the MB molecule, and finally oxidized it to CO2 and H2O. This study successfully prepared commercially applicable Fenton-like catalysts and explored their optimal reaction conditions. This provides a technical reference for wastewater treatment.

Synergistic oxidation of levofloxacin in electro-peroxone system with enhanced in-situ N, P self-standing carbon cathode derived from Chlorella: In-situ H2O2 generation, peroxone activation and catalytic ozonation

An intrinsic N, P self-standing carbon block (NP SSCB) cathode prepared with Chlorella as a precursor was used to enhance the electro-peroxone (EP) process under acidic conditions through a temperature modulation strategy. The results exhibited that NP SSCB-800-EP had a synergistic effect on levofloxacin (LEV) degradation by 42.1% and produced in situ H2O2 up to 70 mg L−1. Quenching and EPR tests confirmed that ·OH, HO2·/O2·- and 1O2 were responsible for degradation of LEV. The quantitative results showed that EP process produced radicals in the order of 1O2> HO2·/O2·->·OH. X-ray photoelectron spectroscopy (XPS) and density functional theory (DFT) calculations under acidic conditions confirmed that pyridinic N, pyrrolic N, C-O-P and the defects were active sites for in-situ H2O2 production. Furthermore, pyridinic N, pyrrolic N and C-P-O groups contributed to the reaction of H2O2 with O3 to form HO2/O2·- and O3·- intermediates, free from overcoming the limitations associated with traditional HO2− production. Graphitic N and C3-PO were possible active sites for triggering the 1O2 and *Oad production. This work provides a new strategy for the preparation of self-standing carbon-based cathodes and achieves multiple functions of EP process under acidic conditions, presenting new insights into structure-functional relationships in carbon-based advanced oxidation processes.

Analysis of factors influencing on Electro-Fenton and research on combination technology (II): a review.

The principle of Fenton reagent is to produce ·OH by mixing H2O2 and Fe2+ to realize the oxidation of organic pollutants, although Fenton reagent has the advantages of non-toxicity and short reaction time, but there are its related defects. The Fenton-like technology has been widely studied because of its various forms and better results than the traditional Fenton technology in terms of pollutant degradation efficiency. This paper reviews the electro-Fenton technology among the Fenton-like technologies and provides an overview of the homogeneous electro-Fenton. It also focuses on summarizing the effects of factors such as H2O2, reactant concentration, reactor volume and electrode quality, reaction time and voltage (potential) on the efficiency of electro-Fenton process. It is shown that appropriate enhancement of H2O2 concentration, voltage (potential) and reaction volume can help to improve the process efficiency; the process efficiency also can be improved by increasing the reaction time and electrode quality. Feeding modes of H2O2 have different effects on process efficiency. Finally, a considerable number of experimental studies have shown that the combination of electro-Fenton with ultrasound, anodic oxidation and electrocoagulation technologies is superior to the single electro-Fenton process in terms of pollutant degradation.

Next-generation of a Fe-Ce double variable-valence metals modulated high-efficiency nanozyme

High catalytic efficiency is the continuous pursuit for the preparation of high-performance nanozymes. In this work, a Fe-Ce double variable-valence metals nanozyme (Fe-Ce-MOL) was constructed based on metal–organic layers (MOLs), a class of two-dimensional metal–organic frameworks with ultra-thin structure, for peroxidase (POD) mimicking. Fe-Ce-MOL exhibited significantly enhanced catalytic activity in comparison with that of natural horseradish peroxidase and single variable-valence metal nanozyme (Fe-Zr-MOL). Density functional theory calculations demonstrated that the Fe site of Fe-Ce-MOL had a stronger adsorption effect on H2O2 compared to Fe-Zr-MOL and more heat was released in the catalytic reaction of H2O2, making the reaction easier to occur. Moreover, there was more electron transfer between Fe and Ce double variable-valence metals, also accelerating the catalytic process. These results indicated that the ultra-thin structure of Fe-Ce-MOL provided more catalytic sites and Fe and Ce double variable-valence metals could regulate the process of oxidation–reduction and promote the efficiency of electron transfer synergistically. As a proof of concept, the proposed Fe-Ce-MOL was used in biosensing and antibacterial applications. These results suggested that Fe-Ce-MOL had great potential as a new-generation nanozyme. This work pioneers a new approach for the rational design of highly efficient nanozymes.

Based on polydopamine-coated metal organic framework multifunctional nanoplatform for enhanced photothermal/sonodynamicand treatment combined with checkpoint blockade therapy

To overcome the low efficacy of sonodynamic therapy (SDT) caused by hypoxia in the tumor microenvironment, we developed a multiple anti-tumor nanoplatform with synergistic SDT, photothermal therapy (PTT), and ferroptosis effects. PCN-224@FcCaO2/Mn/dihydroartemisinin/imiquimod/PDA (PFC) was prepared by modified with dihydroartemisinin (DHA), imiquimod (R837), CaO2, ferrocene (Fc) and Mn2+ on the PCN-224 (Cu) to achieve self-replenishment of H2O2/O2 and GSH consumption. FcCaO2 decomposed into H2O2 in the tumor microenvironment, triggering the Fenton effect to produce OH, and Cu2+ reduced the potential loss of OH by the depletion of GSH. Under ultrasonic (US) and laser irradiation, PFC exhibits exciting PTT and SDT effects from polydopamine (PDA) and PCN-224. Mn2+ not only promoted the reaction of H2O2 to produce O2 to effectively enhance SDT but also induced tumor cell apoptosis by Mn2+ combined with DHA. PFC induced ferroptosis via Fe interaction with DHA to produce ROS and reduce the expression of GPX4. The released R837 and tumor-associated antigens from SDT/PTT can produce damage associated molecular patterns (DAMPs), which can initiate adaptive immune responses to kill cancer cells, and released again to promote the tumor immune cycle. What's more, SDT/PTT and ferroptosis combined with aPD-L1 can effectively suppress both primary and distant tumor growth.

Fe-single-atom catalysts boosting electrochemiluminescence via bipolar electrode integrated with its peroxidase-like activity for bioanalysis

Multifunctional single-atom catalysts (SACs) have been extensively investigated as outstanding signal amplifiers in bioanalysis field. Herein, a type of Fe single-atom catalysts with Fe-nitrogen coordination sites in nitrogen-doped carbon (Fe–N/C SACs) was synthesized and demonstrated to possess both catalase and peroxidase-like activity. Utilizing Fe–N/C SACs as dual signal amplifier, an efficient bipolar electrode (BPE)-based electrochemiluminescence (ECL) immunoassay was presented for determination of prostate-specific antigen (PSA). The cathode pole of the BPE-ECL platform modified with Fe–N/C SACs is served as the sensing side and luminol at the anode as signal output side. Fe–N/C SACs could catalyze decomposition of H2O2 via their high catalase-like activity and then increase the Faraday current, which can boost the ECL of luminol due to the electroneutrality in a closed BPE system. Meanwhile, in the presence of the target, glucose oxidase (GOx)-Au NPs-Ab2 was introduced through specific immunoreaction, which catalyzes the formation of H2O2. Subsequently, Fe–N/C SACs with peroxidase-like activity catalyze the reaction of H2O2 and 4-chloro-1-naphthol (4-CN) to generate insoluble precipitates, which hinders electron transfer and then inhibits the ECL at the anode. Thus, dual signal amplification of Fe–N/C SACs was achieved by increasing the initial ECL and inhibiting the ECL in the presence of target. The assay exhibits sensitive detection of PSA linearly from 1.0 pg/mL to 100 ng/mL with a detection limit of 0.62 pg/mL. The work demonstrated a new ECL enhancement strategy of SACs via BPE system and expands the application of SACs in bioanalysis field.

Thermal rate constants and kinetic isotope effects of the H + H2O2 reactions: barrier height and reaction energy from single- and multireference methods.

One of the more significant sub-mechanisms of H2/O2 combustion involves the reaction of hydrogen peroxide with hydrogen atoms (H + H2O2), resulting in the production of OH + H2O (R1) and H2 + HO2 (R2) paths. Previous experimental and ab initio calculations reveal some variations in the barrier height for (R1). To improve the energetics of both (R1) and (R2), single reference and multireference ab initio methods are employed, and the rate constants and H/D kinetic isotope effects (KIEs) are calculated as a function of temperature. For (R1), the best results for the barrier height and reaction energies computed with the CASPT2(15,11)/aug-cc-pV6Z are 5.2 and - 70.3kcal.mol-1, respectively. CCSD(T)/aug-cc-pV5Z + CV (core-valence) calculations for (R2) give 9.7 and - 15.6kcal.mol-1 to those parameters. The CVT/SCT rate constants of both paths agree well with the fitted rate constants from uncertainty-weighted statistical analysis of the 14-mechanism of H2/O2. The kinetic isotopic effect (kH/kD) for the reaction D + H2O2 → DH + HO2 was found to be 0.47, which is in excellent agreement with the experimental value of 0.43. The structures of reactants, transition state, and products of (R1) and (R2) are calculated with the aug-cc-pVTZ basis set and M062X DFT, CCSD(T), and CASSCF methods. The barrier heights and reaction energies of (R1) and (R2) are computed using the M06-2X, CCSD(T), MRCI, and CASPT2 methods and various basis sets. The rate constants are calculated with the variational transition state theory including multidimensional tunneling corrections (VTST-MT), with potential energy surfaces built by the M06-2X/aug-cc-pVTZ approach.

Immobilized enzymatic membrane surfaces for biocatalytic organics removal and fouling resistance

This research reported on the immobilization of environmentally friendly enzymes, such as horseradish peroxidase (HRP) and laccase (L), along with the hydrophilic zwitterionic compound l-DOPA on nano-filtration (NF) membranes. This approach introduced biocatalytic membranes, leveraging combined effects between membranes and enzymes. The aim was to systematically assess the efficacy of the enzymatic modified membrane (HRP-NF) in degrading colors in the wastewater, as well as enhancing the membrane resistance toward organic fouling. The enzymatic immobilized membrane demonstrated 96.3 ± 1.8% to 96.6 ± 1.9% removal of colors, and 65.2 ± 1.3% to 67.2 ± 1.3% removal of TOC. This result was underpinned by the insights obtained from the radical scavenger coumarin, which was employed to trap and confirm the formation of PRs through the reaction of enzymes and H2O2. Furthermore, membranes modified with enzymes exhibited significantly improved antifouling properties. The HRP-NF membrane experienced an 8% decline in flux, while the co-immobilized HRP-L-NF membrane demonstrated as low as 6% flux decline, contributed by the synergistic effect of increased hydrophilicity and biocatalytic effects. These findings confirmed that the immobilized enzymatic surface has added function of degrading contaminants in addition to separation function of nanofiltration membrane. These l-DOPA-immobilized enzymatic membranes offered a promising hybrid biocatalytic membrane to eliminate dyes and mitigate membrane fouling, which can be applied in many industrial and domestic water and wastewater treatment.

A near-infrared fluorescent probe based FRET for ratiometric sensing of H2O2 and viscosity in live cells

Hydrogen peroxide (H2O2) and viscosity play vital roles in the cellular environment as signaling molecule and microenvironment parameter, respectively, and are associated with many physiological and pathological processes in biological systems. We developed a near-infrared fluorescent probe, CQ, which performed colorimetric and ratiometric detection of H2O2 and viscosity based on the FRET mechanism, and was capable of monitoring changes in viscosity and H2O2 levels simultaneously through two different channels. Based on the specific reaction of H2O2 with borate ester, CQ exhibited a significant ratiometric response to H2O2 with a large Stokes shift of 221 nm, a detection limit of 0.87 μM, a near-infrared emission wavelength of 671 nm, a response time of 1 h, a wide detection ranges of 0.87–800 μM and a high energy transfer efficiency of 99.9 %. CQ could also recognize viscosity by the TICT mechanism, and efficiently detect viscosity changes caused by food thickeners. More importantly, CQ could successfully detect endogenous/exogenous H2O2 and viscosity in live HeLa cells, which was expected to be a practical tool for detecting H2O2 and viscosity in live cells.

Enhancing the treatment efficiency of three-dimensional electro-Fenton system for organic wastewater: An analysis from the comprehensive perspective of H2O2 generation

For optimizing H2O2 generation in three-dimensional electro-Fenton (3D-EF) to enhance efficacy in treating organic wastewater, a comprehensive analysis from various perspectives of H2O2 generation was provided. By quantifying the generation of H2O2 within the reaction system and comparing it with the changing trends in organic wastewater treatment efficiency, a close correlation between H2O2 generation and organic wastewater treatment was revealed. A reliable and accurate kinetic model for H2O2 generation was formulated by building upon the reactions of H2O2 with electrodes and coexisting substances. The mathematical formulation of the kinetic model pinpointed pivotal factors influencing H2O2 generation, including current density, dissolved oxygen concentration, and the Fe catalytic activity substance. The experimental data and fitted results under various conditions indicated that the model could reasonably and accurately describe the relationship between pivotal factors and the quantity of generated H2O2. Crucial strategies were proposed to enhance H2O2 generation in the 3D-EF system by drawing from the impact rules of pivotal factors on H2O2 generation. These strategies primarily focused on improving electrode performance, optimizing reactor conditions, and refining particle electrode preparation processes.

Multifunctional Gold Nanozyme-Engineered Amphotericin B for Enhanced Antifungal Infection Therapy.

Chronic wounds of significant severity and acute injuries are highly vulnerable to fungal infections, drastically impeding the expected wound healing trajectory. The clinical use of antifungal therapeutic drug is hampered by poor solubility, high toxicity and adverse reactions, thereby necessitating the urgent development of novel antifungal therapy strategy. Herein, this study proposes a new strategy to enhance the bioactivity of small-molecule antifungal drugs based on multifunctional metal nanozyme engineering, using amphotericin B (AmB) as an example. AmB-decorated gold nanoparticles (AmB@AuNPs) are synthesized by a facile one-pot reaction strategy, and the AmB@AuNPs exhibit superior peroxidase (POD)-like enzyme activity, with maximal reaction rates (Vmax) 3.4 times higher than that of AuNPs for the catalytic reaction of H2O2. Importantly, the enzyme-like activity of AuNPs significantly enhanced the antifungal properties of AmB, and the minimum inhibitory concentrations of AmB@AuNPs against Candida albicans (C. albicans) and Saccharomyces cerevisiae (S. cerevisiae) W303 are reduced by 1.6-fold and 50-fold, respectively, as compared with AmB alone. Concurrent in vivo studies conducted on fungal-infected wounds in mice underscored the fundamentally superior antifungal ability and biosafety of AmB@AuNPs. The proposed strategy of engineering antifungal drugs with nanozymes has great potential for enhanced therapy of fungal infections and related diseases.