The present study pertains to the flexural behavior of RC beams with openings and non-metallic (GFRP) reinforcement. The main goal of preferring GFRP reinforcement over the conventional steel reinforcement was to safeguard the beams against the reinforcement corrosion. The presence of multiple regular transverse openings throughout the beam length increases the susceptibility of reinforcing bars to corrosion as the larger contact area in these beams with the outside environment increases the ingress of corrosive agents. Within the scope of the study, a total of 8 RC beams, including two reference beams without web openings, were tested under four-point bending. The test parameters were the flexural reinforcement ratio, the presence of short stirrups in the chords, and the presence of diagonal reinforcement spiraling around the openings. Since GFRP stirrups are difficult to bend, each stirrups was formed by connecting four individual FRP bars around the longitudinal bars. The opening circular geometry was adopted to avoid stress concentrations around the sharp corners of opening and to facilitate the placement and fixing of different schemes of reinforcement in the beams. The present tests depicted that the diagonal reinforcement around the openings have considerable contribution to the flexural behavior of RC beams with GFRP reinforcement and with multiple regular transverse openings. The RC beams with openings were able to approach their analytical flexural capacities in the presence of diagonal reinforcement for both moderately and heavily reinforced beam groups. The analytical deflection predictions of GFRP-RC beams with openings showed a good agreement with experimental data.