Rattus Novergicus Strain Wistar Research Articles

Anti-Inflammatory Effects of Ripe Areca Nut Ethyl Acetate Fraction in Wistar Rats with Knee Joints Osteoarthritis

Osteoarthritis (OA) is the most common form of arthritis. Meloxicam are one of NSAIDs commonly used that have many side effects. Areca nut (Areca catechu L), which contains flavonoids, can provide anti-inflammatory effects. This study aimed to determine the potential anti-inflammatory effect of ethyl acetate fraction from ripe areca nut in OA rat model. Twenty five of adults, male Rattus novergicus strain Wistar were induced to OA with Monosodium Iodoacetate (MIA), then divided into five groups that each group were given ethyl acetate fraction of ripe areca nut doses of 15mg/kgBW, 30mg/kgBW, and 60mg/kgBW, meloxicam 1.35mg/kgBW and aquadest as negative control for 14 days. Diameter of the knee joint is measured by digital metric kaliper to assess joint edema. The Histopathological examination with Haematoxyllin-Eosin stain to assess degree of inflammation on synovial. Among the areca groups, dose 30mg/kgBW had knee joint diameter reduced better and lower synovitis score. In conclusion, ethyl acetate fraction of the ripe areca nut group has anti inflammation effect in OA model but not dose-dependent.

The Effect of Calorie Restriction on The Growth of Adipose Tissue in Mice

The aging process can be slowed by reducing the calorie intake consumed by the body by calorie restriction or fasting. This study aimed to analyze the effect of calorie restriction on adipose tissue in mice. The experimental research design uses 12 months old Rattus Novergicus Strain Wistar, weighing 250–300 grams. The intervention was carried out for 14 days, then the average weight of subcutaneous and visceral fat was measured using Kruskal Wallis analysis and ANOVA. Based on the four treatment groups, the mice fasting and given a high-calorie diet (group 4) had the highest average fat weight value of 4.02 grams in the subcutaneous and 3.55 grams in the visceral area. Meanwhile, in group 1, mice that were not fasting and did not get a high-calorie diet had the lowest average fat weight values, namely 2.14 grams in the subcutaneous area and 2.72 grams in the visceral area. Based on the test results, it was found that there was no difference between subcutaneous (p=0.121) and visceral (p=0.443) fat tissue in all groups. Calorie restriction is beneficial for delaying aging and increasing mammal life span, but intermittent calorie restriction does not affect adipose tissue.

Effect of intravenous glutamine on the protein expression changes, caspase-12 and apoptosis in renal tubulus russ male, rattusnovergicusstrain wistar, exposed by cisplastin

Cisplatin efficacy is dependent on dose. The cisplatin-induced apoptosis pathway can go through the ER stress pathway which will activate Bax and Bak (proapoptosis) causing calcium homeostasis disorders to occur. Glutamine protective effect on caspase-12 expression on the prevention of apoptotic events in renal proximal tubular epithelial cells is unknown. This further study analyzes the effects of intravenous glutamine on caspase- 12 expression in the apoptosis of renal proximal tubular cells of male white rats exposed to cisplatin. The control group (P0) consisted of 10 individuals, group P1 on day 7 was given a single dose of 20mg / kg of intraperitoneal cisplatin injection, group P2 on day 1-7 was given intravenous glutamine injection of 100mg / kg on day 7 also given a single intraperitoneal cisplatin injection 20mg / kg. The number of cells expressing caspase-12 and the number of apoptotic cells was observed after 72 hours. Caspase-12 expression and apoptosis data were collected for processing and analysis. There is a significant effect (p <0.0001) in giving intravenous glutamine on caspase-12 expression in renal proximal tubular cell apoptosis. Glutamine can inhibit the expression of caspase-12, and the incidence of proximal tubular cell apoptosis were exposed by cisplatin.

PENGARUH SEDIAAN DEKOK DAUN ZAITUN (Olea europaea L.) TERHADAP PENURUNAN TEKANAN DARAH PADA TIKUS PUTIH GALUR WISTAR (Rattus norvegicus strain wistar) JANTAN YANG DIINDUKSI NATRIUM KLORIDA 5,5%

According toWHOhypertension about 26.4% of world population who have suffered hypertension. It wouldprobably increase up to 29.2% in 2025. Usually,hypertension medical care uses convensional medicine, however itpossessessome side effects and could causecomplication.For that reason, herbal medicine is needed for safer andmore effective purpose. One of herbal medicines is olive leaf (Olea europea L).It aimed to figure out effect of oliveleaf extract (Olea europaea L.) on declining blood pressurein whitemale rat wistar strain (Rattus novergicus strainwistar) after 5.5% Natrium Chloride (NaCl) induction.24 of rats were divided into 4 groups of treatments and 5.5%NaCl inductions. Group I, as a control withaquadest treatment, Group II was inducted with 540 mgs of olive leafextract, Group III was inducted with 1080mgs of olive leaf extract, and Group IV was inducted with 2160 mgs ofolive leaf extract. Data were Analyzed byOne way ANOVA then followed with Post-hoc test using LeastSignificant Differences (LSD) test. From the group I, the mean of pretest was 117.83/150.83mmHg and the meanof post test was117.83/141.67 mmHg. Group II, the mean of pretest was 123.67/156,00 mmHg and the mean of posttest was87.00/116,00 mmHg. Group III, the mean of pretest was 123.50/156.83 mmHg and the mean of post testwasand the mean of post test was 81.67/112,50 mmHg. Group IV the mean of pretest was 119.00/156.50 mmHgandthe mean of post test was 80.00/105.00 mmHg.There were effects of olive leaf extract (Olea europaea L.) in 540mgs, 1080 mgs and 2160 mgs toblood pressure declining in white male rat wistar strain (Rattus novergicus strainwistar) after 5.5% NatriumChloride (NaCl) induction. The higest blood pressure declining was in Group IV of 2160mg olive leaf extractinduction.

Therapeutic Effect of Statins and LPS Antibody on Proinflammatory Mediator and Biochemical Markers of Sepsis Rat Model Induced by E. coli

This study aims to determine the effect of statins and LPS antibodies administration combined with E. coli intraperitoneal injection toward proinflammatory mediators (TNF-?, hs-CRP, PCT, and MDA) urea, BUN, and creatinine) and liver function (SGPT, SGOT, and total bilirubin) in sepsis rats. This study used 30 male white rats (Rattus novergicus strain Wistar) divided into 6 groups induced by E. coli bacteria combined with statins, LPS antibody (Ab-LPS), and a combination of statins+Ab-LPS. Examination of proinflammatory mediators (TNF-?, hs-CRP, PCT, and MDA) was carried out by the ELISA method through the examination of biochemical markers namely renal function (urea, BUN, and creatinine) and liver function (SGPT, SGOT, and total bilirubin) were analyzed by using the autoanalyzer. The data obtained were statistically analyzed using the T-test. The result shows that E. coli+statins gave significant changes on the MDA level at 0th hour administration and TNF-? and hs-CRP at 3rd hour administration. The E. coli+Ab-LPS treatment showed significant changes at the 0th hour administration for MDA, creatinine, and total bilirubin and at the 3rd hour administration for MDA. The E. coli+statins+Ab-LPS treatment showed significant changes in MDA, creatinine, and total bilirubin at 0th hour administration and on creatinine and total bilirubin at 3rd hour administration.

The Effect of Glutamine Supplementation on Ileum Histopathology of Rats with Acute and Chronic EPEC-Induced Diarrhea

BACKGROUND: Glutamine is a non-essential amino acid as the main fuel in the gastrointestinal mucosa. By its various gastrointestinal functions, glutamine is thought to increase the protection of the intestinal mucosa against local or systemic injury from diarrhea. 
 AIM: This study aims to determine the relationship between glutamine supplementation and ileum histopathology in rats with acute and chronic diarrhea induced by EPEC.
 MATERIAL AND METHODS: The research was conducted in an experimental laboratory with a randomized posttest-only control group design. A total of 30 Rattus novergicus strain Wistar were divided into 5 groups. The treatment group was induced to have diarrhea using EPEC at a dose of 108 CFU/ml, after each group diagnosed by acute and chronic diarrhea, followed by glutamine supplementation at a dose of 810 mg/200 g for 14 days. The intestinal histopathology of each group was assessed and their levels of inflammation classified based on the Barthel-Mandja inflammatory score.
 RESULT: The result showed that significant differences in inflammation levels between the negative control group, acute diarrhea group, chronic diarrhea group, acute diarrhea group with glutamine, and chronic diarrhea group with glutamine were found (p<0.05). The highest level of inflammation was found in the acute diarrhea group.
 CONCLUSION: This study concluded that glutamine supplementation has an effect on the ileum histopathology of rats with acute and chronic diarrhea.
 Keywords: Enteropathogenic E. Coli, Glutamine, gastrointestinal tract, pathology, ileum.

EFEKTIFITAS EKSTRAK DAUN KELOR (Moringa oleifera lam) DALAM MEMPERBAIKI PROFIL SERUM IRON, SATURASI TRANSFERIN, DAN TOTAL IRON BINDING CAPACITY TIKUS PUTIH JANTAN DIET RENDAH ZAT BESI

Background : Iron deficinecy can cause anemia which affect health and work productivity. Moringa oleifera leaves contain a lot of iron and vitamin C, are thought to increase serum iron levels, transferin saturation, and improve total iron binding capacity.Objectives : To determine the effectiveness of Moringa oleifera lam. leaf extract on serum iron levels, transferin saturation, and total iron binding capacity in white rats (Rattus novergicus strain wistar) given a diet low in iron (Fe).Materials and methods : true experimental research using post test only control group design. The object of theis study was thirty male white mice (Rattus novergicus strain wistar) who were given a low iron diet. The study was divided into negative control group, positive control, treatment groups with a dose of Moringa leaf extract 400 mg/kg BW, 800 mg/kgBW, 1,600 mg/kg/BW, an the ferro fumaras control group (FF) 175 mg/200 BW.Results and discussion : Multivariate manova serum iron 0.001 (p<0.05), TBC 0.19 (P>0.05), and transferin saturation 0.001(p<0.05). In the post Hoc Tukey the mean serum iron and transferin saturation appeared to differ significant in the negative control group with all the other treatment groups, but did not show a significant diffrence beetwen the positive control group, FF Group, and the treatment groups.Conclusion : The adminstration of Moringa oleifera leaf extractwas less significant in improving serum iron levels, transferin saturation, and total iron binding capacity of male white mice induced by a low iron diet.

EFEKTIVITAS PEMBERIAN EKSTRAK IKAN HARUAN (Channa striata) TERHADAP JUMLAH NEUTROFIL PADA PROSES PENYEMBUHAN ULKUS TRAUMATIKUS

<p><strong><em>Background:</em></strong><em> </em><em>Channa striata contains important compounds in the process of tissue synthesis and important role in wound healing, such as albumin, zinc (Zn), copper (Cu), iron (Fe) and unsaturated fat acids. <strong>Purpose :</strong> To determine the effect of Channa striata extract to the amount of neutrophil in healing process of traumatic ulcer. <strong>Material and Method :</strong> The design of this study used randomized post test only control group design. 18 wistar rats were divided into 6 groups, i.e: K-1 (no treatment for 1 day), K-3 (no treatment for 3 days), K+1 (giving hyaluronic acid 0.2% for 1 day), K+3 (giving hyaluronic acis 0.2% for 3 days), P1 (giving Channa striata extract 100% for 1 day), P3 (giving Channa striata extract 100% for 3 days). <strong>Results :</strong> There was a decrease in the number of neutrophil in the treatment group. The mean and standart deviation of the number of neutrophil in the K-1 group (75,96±3,65), K-3 (50,76±2,04), K+1(72,96±2,97),K+3(45,06±2,83),P1(54,20±1,31),P3(32,50±,85). <strong>Conclusion :</strong> The using of Channa striata extract 100% for 3 days are the most effective in decreasing the amount of neutrophil in healing process of traumatic ulcer of Rattus Novergicus Strain Wistar.</em></p>

The Effect of Belimbing Wuluh (Averrhoa Bilimbi L.) and Red Guava (Psidium Guajava L.) Extract on Fasting Blood Glucose Levels of Diabetic Wistar Rats Model

Diabetes mellitus is a metabolic disease with special characteristic which increased blood glucose level (hiperglicemic), its happens because of the abnormality of insulin secretion, insulin mechanisme or both of them. Belimbing wuluh and red guava extract can become alternative functional drink that contain rich of vitamin C to help controlling blood glucose level. The aimed of this study was to determined the effect of belimbing wuluh and red guava extract on blood glucose levels of Rattus novergicus strain Wistar diabetic model. This research was an true experimental study with a randomized post-test control group design. Twentyfour (24) male wistar strain rats weighing 200-250 grams, aged 2-3 months, were divided into 4 groups. The control group (K-) non-diabetic rats, the control group (K+) diabetic rats given standard diet, and the treatment group diabetic rats given standard diet and belimbing wuluh and red guava extract intervetion doses 10,7 ml/day (P1) and 21ml/day (P2). Measurement of pretest posttest fasting blood glucose levels was carried out using biolyzer. The data were analyzed by One Way Anova test. The results showed that there were significant differences in delta pretest and posttest fasting blood glucose levels between all groups. Blood glucose levels decreased at 55.6%. In conclusion, belimbing wuluh and red guava extract 21ml/day most effectively decreased fasting blood glucose levels in diabetic wistar rats moldel.
 
 Keywords: belimbing wuluh, blood glucose level, diabetes mellitus, red guava

Effect of Antihipertensive Drugs And Banana (Musa Sp.) To Potassium Serum Levels of Hypertensive Wistar Rats Model

Consumption of antihypertensive drugs has the potential to cause drug and food interactions. Captopril/lisinopril can increase potassium levels by inhibiting the activity of renin angiotensin aldosterone. If captopril/lisinopril is consumed together with bananas, there is a risk of hyperkalemia because bananas are a high pottasium fruits. The aimed of this study was to determined the effect of captopril/lisinopril given with banana (Musa Sp) on potassium serum levels of Rattus novergicus strain Wistar hypertension model. This research was an experimental study with a randomized post-test control group design.Thirty (30) male wistar strain rats aged 2-3 months, weighing 200-250 grams were divided into 6 groups. The control group (K-) non-hypertensive rats, the control group (K+) hypertension rats+standard diet, the treatment group (P1) hypertension rats + standard diet + captopril 2.25 mg/kgBB, the treatment group (P2) hypertensive rats + standard diet + lisinopril 0.6 mg/kgBB, treatment group (P3) hypertension rats + standard diet + captopril with banana and treatment group (P4) hypertension rats + standard diet + lisinopril with bananas. Measurement of potassium serum levels was carried out using electrolyte analyzer. Furthermore, the data were analyzed by One Way ANOVA test. The results showed that there were no significant differences in potassium serum levels between all groups. In conclusion, both of captopril 2.25 mg/kgBB and lisinopril 0.6 mg/kgBB given together with banana 9g/kgBB for 3 days hasn’t caused drug and food interactions.
 Keywords: banana fruits, captopril, lisinopril, potassium serum

Effect of Ipomoea Batatas L. Leaves Extract on Percentage Healing of Traumatic Ulcer in Oral Mucosa (Rattus Novergicus Strain Wistar)

Effect of Ipomoea Batatas L. Leaves Extract on Percentage Healing of Traumatic Ulcer in Oral Mucosa (Rattus Novergicus Strain Wistar)

Pengaruh Pemberian Alga Coklat (Sargassum sp.) Terhadap Enzim Katalase Kelenjar Submandibularis Tikus Rattus Norvegicus Strain Wistar Akibat Iradiasi Linear Energy Transfer (LET) Rendah

Background : Background: Intraoral radiography use some lower LET (Linear Energy Transfer) and could penetrate submandibular salivary gland. Radiography have negative impact which is decrease catalase enzyme of human body. Brown algae (Sargassum sp.) has a flavonoid antioxidant, polysaccharides as Fucoidan and alginat (Na-alginat) can be used for immunomodulator, antioxidative and activation modulation of immune. Purpose: To knowing effectiveness of brown algae (Sargassum sp.) on activity catalase enzyme submandibular salivary gland Rattus Novergicus strain Wistar with irradiation low LET. M aterial and Methods : 28 samples of Rattus Novergicus strain Wistar, weight 200gr, age 2-3months, gender male, sample divide into 4 groups, K1 (control with brown algae dosage 0,018mg/kgbw) K2 (use brown algae and irradiation 4 times), K3 (use brown algae and irradiation 8 times), K4 (use brown algae and irradiation 14 times). Brown algae been given 7days before apply irradiotion on day 8, then did euthanasia and took submandibular salivary gland. After that did measurement activity of catalase enzyme and counted by spectrophotometer with 240 λ. Result : Data were analyze by Shapiro-wilk , One Way ANOVA and Bonferroni . The activity of catalase enzyme have increased; 0,2586 ± 0,1050 (K1), 0,2595 ± 0,0630 (K2), 0,3252 ± 0,1663 (K3), 0,3668 ± 0,0852 (K4) but theres no significant differences activity of catalase enzyme between one group to other group. Conclusion : Brown algae dosage 0,018mg/kgbw can’t increase activity of catalase enzyme on Rattus Novergicus strain Wistar.

Diagnostic Test of Urine Proteome during Atherosclerotic Process Among White Rat (Rattus Novergicus Strain Wistar) Induced by Atherogenic Diet

Introduction: Around twenty five million people in United State have at least one complications of atherosclerosis such as cardiovascular disease. Therefore screening in atherosclerotic process is important. Atherosclerosis is a multistage process that begins with damage to the endothelium due to LDL exposure. Angiography Coronary is the primary diagnostic tool for atherosclerosis. Imaging, as one way of non-invasive diagnosis, have limitation in sensitivity, specificity, and cost. Proteome urine analysis is alternative for atherosclerosis diagnostic.Objective: The aim of this study was analyse diagnostic performance of urine proteome among white rat (Rattus Novergicus strain wistar) induced by atherogenic diet.Material and methods: This study was quasi experimental in laboratory with randomized posttest only for control group design. The rat samples were divided to two groups i.e. group with atherogenic diet and non-atherogenic diet. Each group consisted of 10 rats. The treatment was done for 15 weeks. Urine sample collection was performed in week 16 of treatment. Urine proteome, collagen type I, was analyzed by ELISA to obtain biomarkers of atherosclerosis. Then determined sensitivity and specificity of the proteome urine analysis was compared with histopathology findings of the aortic arch and its branches.Result: In week 16, the researchers collected sample from 16 rats i.e. 11 rats with atherogenic diet and 5 ratswith non-atherogenic diet. Urine proteome, collagen type I, had area under the receiver operator with the characteristic curve of 98.2% (95% IK 92.6%-100%), p 0,003. The bestcut offis 0.9025 ng/ml with sensitivity of 100% and specificity of 90.9%.Conclusion: Urine proteome analyse, collagen type I, had high accuracy for atherosclerosis diagnostic.Bangladesh Journal of Medical Science Vol.15(4) 2016 p.522-528

LBPS 01-04 SUB CHRONIC TOXICITY EVALUATION OF POLYSACCHARIDE PEPTIDE GANODERMA LUCIDUM IN AORTA

Objective: Atherosclerosis as bakbone of Cardiovascular disease have increase burdenly in world wide. Dyslipidemia, oxidative process and inflammation either free radical were cornerstone to advancing atherosclerotic process. Ganoderma Lucidum has acceptance and widely use since 2000 years ago in asia. Polysaccharide peptide Ganoderma Lucidum have numerous effect as anti inflammation, anti oxidant, anti lipidemia, and anti diabetic. The aim for this study is to determined and examined sub chronic toxicity of Polysaccharide peptide administration in aorta rattus novergicus strain wistar. Design and Method: This study design as animal experimental study with control in 4 group of rattus novergicus strain wistar both male and female rats and give Polysaccharide peptide with dose of 0, 300, 600 and 1200 mg/kg once daily for 90 days. Parameter measured were clinical symptoms of toxicity, evaluation of histopathological structure and blood chemistry with descriptive statistic with t-test and non parametric with ANOVA One way with p < 0.05 was significant. Furthermore Tuckey test is used to determine between 2 groups. Results: Polysaccharide Peptide Ganoderma Lucidum affect in physical obervation clinical symptoms of toxixity and gross organ were no significant toxicity symptoms and from gross examination found that condition of aortic organ remain unchanged and from organ pathology found no change in histopathological aortic organ even after a dose of 1200 mg/kg. Conclusions: Polysaccharide peptide ganoderma lucidum has negatve toxicity in sub chronic test and there are not found any changes in morphology of both group. Polysaccharide peptide has good safety profile to next use in human trial.

EFEK ISOFLAVON FITOESTROGEN DARI EKSTRAK Pueraria lobata TERHADAP MEMORI DAN AKTIVITAS KOLINERGIK DI HIPPOKAMPUS CA1 PADA TIKUS HIPOESTROGEN

Data from retrospective and case-conttrole showed that estrogen replacement therapy (ERT) could prevent ang delayed againstAlzheimer Disease (AD). The using of ERT is still controversial because of their long-term side effect. Isoflavon as a part of phytoestrogenhas potential effect as an alternative substitute for ERT. The aim of this study is to examine the effect of phytoestrogen from Pueraria lobataextract in increasing memory’s function and whether it occur by the increased of cholinergic activity in CA1 hippocampus. Exploration studyperformed by three step. First, determined genistein and daidzein concentration in Pueraria lobata extract, as basic of dose determining.Second, oophorectomy performed to made a hypoestrogenic rat’s model; and third, determined method memory’s function in rats. This studyused twenty five female Rattus novergicus strain Wistar, 10 -11 weeks of age, five rats were normal rats (N) as first group and twenty ratswere hypoestrogenic rats for 2nd, 3rd, 4th, and 5th groups. All of the hypoestrogenic rat were devided into 4 groups, each 5 rats. Hypoestrogenicrats group without Isoflavon Genistein Daidzein supplement (OVX), hypoestrogenic rats group with Isoflavon Genistein Daidzeinsupplement (OVX+IGD) dosage of 15, 30, and 60 mg/kgweight/day each. The IGD was given for 21 days. The Morris Water Morris(MWM) test was performed in 17th, 18th, 19th, 20th, and 21st day. On 22nd day rats were killed. Frozen section of the brain was performedimmediately. Cholinergic activities on CA1 hippocampus were examined with AChE staining. A significant difference was found onmemory’s function and cholinergic activities on CA1 hippocampus between OVX and OVX+IGD groups at all doses (p value = 0,000 ofeach). No significant difference on IGD effect toward brain weight (p=0,936) and pyramidal cell number on CA1 hippocampus (p=0,961).It was suggest that increased memory’s function on OVX+IGD groups were caused by the increased of cholinergic activities on CA1hippocampus.Key word : Isoflavones; phytoestrogen; Genistein; Daidzein; Pueraria lobata; Memory; cholinergic.

PENGARUH PEMBERIAN DIET TINGGI KALSIUM TERHADAP PENURUNAN BERAT BADAN PADA Rattus novergicus galur wistar

ABSTRACT Calcium diet has an important role in energy metabolism regulation especially intracellular Ca2+ which is the key tarrange the metabolism of adiposity fat and triasilgliserol save. When intracellular Ca2+ increase it makes the stimulatioof gen lipogenic and lipogenesis also forcing of lipolysis and finally increasing the fat fitting and increasing adiposity. Thlow level of calcium absorbtion increases the production of calsitirol which could stimulate adiposity Ca2+ influx and thconcequance is the increase of adiposity, so it needs high calcium diet to delay lipogenesis increase of lipolysis, lipioksidaxion, to reduce fat adiposity and weight. The research used  pretest – postest control group design. Trial animawere male rat of Rattus novergicus strain wistar. The research consisted of 2 phases. The first phase was increasing thrat weight by giving normal diet with calcium of 0,4% for all kinds of rats for 4 weeks. The second phase by giving calciumdiet with different doses that is  group A as control group (0,4% Ca as normal diet), group B 0,8% Ca (Ca as normal diet +0,4% Ca as CaCO3), group C 1,2% Ca (Ca as normal diet + 0,8% Ca as CaCO3) and  group D 1,6% Ca(Ca as normadiet + 1,2% Ca as CaCO3) for 4 weeks. To find out the difference of calcium diet it used statistical test Oneway Anova