Effect of intravenous glutamine on the protein expression changes, caspase-12 and apoptosis in renal tubulus russ male, rattusnovergicusstrain wistar, exposed by cisplastin

Abstract

Cisplatin efficacy is dependent on dose. The cisplatin-induced apoptosis pathway can go through the ER stress pathway which will activate Bax and Bak (proapoptosis) causing calcium homeostasis disorders to occur. Glutamine protective effect on caspase-12 expression on the prevention of apoptotic events in renal proximal tubular epithelial cells is unknown. This further study analyzes the effects of intravenous glutamine on caspase- 12 expression in the apoptosis of renal proximal tubular cells of male white rats exposed to cisplatin. The control group (P0) consisted of 10 individuals, group P1 on day 7 was given a single dose of 20mg / kg of intraperitoneal cisplatin injection, group P2 on day 1-7 was given intravenous glutamine injection of 100mg / kg on day 7 also given a single intraperitoneal cisplatin injection 20mg / kg. The number of cells expressing caspase-12 and the number of apoptotic cells was observed after 72 hours. Caspase-12 expression and apoptosis data were collected for processing and analysis. There is a significant effect (p <0.0001) in giving intravenous glutamine on caspase-12 expression in renal proximal tubular cell apoptosis. Glutamine can inhibit the expression of caspase-12, and the incidence of proximal tubular cell apoptosis were exposed by cisplatin.