Abstract
Introduction: Around twenty five million people in United State have at least one complications of atherosclerosis such as cardiovascular disease. Therefore screening in atherosclerotic process is important. Atherosclerosis is a multistage process that begins with damage to the endothelium due to LDL exposure. Angiography Coronary is the primary diagnostic tool for atherosclerosis. Imaging, as one way of non-invasive diagnosis, have limitation in sensitivity, specificity, and cost. Proteome urine analysis is alternative for atherosclerosis diagnostic.Objective: The aim of this study was analyse diagnostic performance of urine proteome among white rat (Rattus Novergicus strain wistar) induced by atherogenic diet.Material and methods: This study was quasi experimental in laboratory with randomized posttest only for control group design. The rat samples were divided to two groups i.e. group with atherogenic diet and non-atherogenic diet. Each group consisted of 10 rats. The treatment was done for 15 weeks. Urine sample collection was performed in week 16 of treatment. Urine proteome, collagen type I, was analyzed by ELISA to obtain biomarkers of atherosclerosis. Then determined sensitivity and specificity of the proteome urine analysis was compared with histopathology findings of the aortic arch and its branches.Result: In week 16, the researchers collected sample from 16 rats i.e. 11 rats with atherogenic diet and 5 ratswith non-atherogenic diet. Urine proteome, collagen type I, had area under the receiver operator with the characteristic curve of 98.2% (95% IK 92.6%-100%), p 0,003. The bestcut offis 0.9025 ng/ml with sensitivity of 100% and specificity of 90.9%.Conclusion: Urine proteome analyse, collagen type I, had high accuracy for atherosclerosis diagnostic.Bangladesh Journal of Medical Science Vol.15(4) 2016 p.522-528
Highlights
Around twenty five million people in United State have at least one complications of atherosclerosis such as cardiovascular disease
The proportion of deaths caused by heart disease was 4.6% from the total cause of deaths from all age2.the diagnosis of atherosclerosis before the occurrence of complications is very important
Atherosclerosis begins with endothelial damage due to exposure to LDL
Summary
Around twenty five million people in United State have at least one complications of atherosclerosis such as cardiovascular disease. Proteome urine analysis is alternative for atherosclerosis diagnostic. Conclusion: Urine proteome analyse, collagen type I, had high accuracy for atherosclerosis diagnostic. According to data from the Ministry of Health of Indonesia, the prevalence of coronary heart disease based on interviews about the symptoms is about 1.5% from the total population in Indonesia. Those diagnosed by doctor is only 0.5%2. Oxidized LDL will activate receptors on leukocytes, especially vascular cell adhesion molecule-1 (VCAM-1) and release proinflammatory cytokines Both of these processes resulted in the recruitment of leukocytes, especially monocytes and lymphocytes
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
