Rats Fed Fish Oil Diet Research Articles

Effect of Coconut Water Extract on the Growth and Serum Biochemical Values of Rats Fed Fish Oil Diet

To justify the safety of ethanolic extract of coconut water (CWE), a study with rats was conducted. Wistar rats fedfish oil diet and rats fed the standard diei of AIN93G groupi were orally administrated 0.8 ml CWE/rat twice per day for 14 days. During experimental period, the behavior and growth of rats were monitored. At the end of experiment, the rats were anaesthetized and the internal organs were taken and measuredfor each weight. Blood was taken from the hearth and then analyzed for serum biochemical values and TBARS. The results, no adverse effects of CWE administration were observed as evaluated by growth pattern, food intake and feces appearance. Serum biochemical values and weight of internal organs of CWE receiving rats were comparable to those of untreated control. SeruniTBARS value of rats fedfish oil was significantly higher than that of those ed standard diet, however, oral administratibn of CWE did not significantly decreaie serum TBARS

Effect of degree of unsaturation in dietary fatty acids on arachidonic acid mobilization by peritoneal macrophages

Cells from rats fed with a tripalmitin diet showed a depletion of phospholipid arachidonate and n-3 fatty acids such as eicosapentaenoic and docosahexaenoic acids (EPA and DHA). In rats fed fish oil diet, a significant reduction in arachidonic acid (AA) content was observed whereas EPA and DHA were incorporated into membranes lipids. These changes in lipid composition of membranes did not affect cellular adherence, phagocytic capability, or [3H]AA incorporation. However, both tripalmitin and fish oil diets induced a decrease in [3H]AA mobilization stimulated by 4 beta-phorbol-12-myristate 13-acetate, A23187, or opsonized-zymosan in rat peritoneal macrophages. These results demonstrate that the antiinflammatory effects of essential fatty acids deficiency or n-3 enrichment diets may be associated with a decreased AA mobilization in resident rat peritoneal macrophages treated with proinflammatory agents.

Antioxidative Effect of Coconut (Cocos nucifera L.) Water Extract on TBARS Value in Liver of Rats Fed Fish Oil Diet

Ethanolic extract of the coconut water showed antioxidant activity in the in vitro assay using an emulsified-aqueous system containing linoleic acid and /3-carotene. To evaluate the possible antioxidative effect in vivo, a study with rats fed fish oil diet, was performed. Oral administration of the coconut water extract (CWE, 340mg/mL) did not influence food intake, daily body weight gain nor average liver weight of rats. It was investigated that the increase of liver TBARS value of rats fed fish oil diet was significantly (P < 0.01) suppressed by oral administration 0.8 ml of the coconut water extract (CWE 340 mg/ml)/rat twice per day during 14 days of experimental period. Oral administration of coconut water extract also tended to suppress the decreasing effect of fish oil diet on liver ascorbic acid. Liver total glutathione of rats fish oil diet was significantly lower than that of rats fed AIN-93G standard diet, but oral administration of the coconut water did not significantly change the liver glutathione. SOD activity in liver homogenate of rats fed fish oil diet and orally administrated by coconut water extract was significantly higher than that of those orallyadministrated by water. The result showed that the antioxidative properties of the coconut water extract have been demonstrated by in vivo assay using rats fed fish oil diet.

Effect of fish oil and coconut oil diet on the LDL receptor activity of rat liver plasma membranes

The influence of 4 weeks treatment with fish oil and coconut oil enriched diets on the chemical composition of rat liver plasma membranes and LDL and on the binding of LDL to liver membranes was investigated. Rats fed fish oil diet showed a total, LDL and HDL plasma cholesterol concentration lower than the values observed in rats fed coconut oil and to a lesser extent lower than those of rats fed standard laboratory diet. LDL of rats on fish oil diet had a relative percentage of cholesterol and phospholipid lower, while that of triacylglycerol was greater. Furthermore, fish oil feeding was associated with a greater concentration of n − 3 fatty acids and a lower arachidonic and linoleic acid content in LDL. Liver plasma membranes isolated from fish oil rats showed a higher percentage of n − 3 fatty acids, while only a trace amount of these fatty acids was found in control and coconut oil fed animals. In binding experiments performed with LDL and liver membranes from fish oil fed rats and control rats, binding affinity ( K d = 3.47 ± 0.93 and 4.56 ± 1.27, respectively) was significantly higher ( P < 0.05) as compared to that found using membranes and lipoprotein from coconut oil fed rats ( K d = 6.82 ± 2.69. In cross-binding experiments performed with fish oil LDL and coconut oil liver plasma membranes or coconut oil LDL and fish oil liver plasma membranes, the LDL binding affinity was comparable and similar to that found in fish oil fed animals. No difference was found in the B max among all the groups of binding experiments. Our data seem to indicate that during fish oil diet the higher binding affinity of LDL to liver plasma membranes might be partly responsible of the hypocholesterolemic action of marine oil rich diet as compared to saturated diet. Furthermore, the modifications of binding affinity induced by changes of LDL and membranes source, suggest that lipoprotein and liver plasma membrane composition may be an important variable in binding studies.

Effect of Dietary Lipids on Accumulation of Pentachlorobenzene

The present experiments were conducted to examine the effect of dietary lipid on the accumulation of pentachlorobenzene (PECB). Male Sprague Dawley rats (4-week-old) were fed diets of 10% or 20% soybean oil, 10% lard, 10% olive oil or 10% fish oil either containing or not containing 50mg% PECB for 2 weeks.Significant reductions of accumulated PECB in perirenal and epididymal fats were observed in rats fed the fish oil diet, while the content in the liver was comparable with those in rats fed other lipid diets. Although fecal excretion of PECB was significantly higher in rats fed the olive oil diet than those in rats fed other diets, the amount was only 6.3% of the estimated daily dose. Induction of cytochrome P-450 by PECB in hepatic microsomes was not observed only in rats fed fish oil diet. On the other hand, triglyceride in the liver and the weight of adipose tissues decreased in rats fed the fish oil diet.The above results suggest that feeding of fish oil may reduce accumulation of fat-soluble xenobiotics in adipose tissues and the mechanism may be related to the metabolism of triglyceride rather than an induction of drug-metabolizing enzyme(s).