Rational Sequence Research Articles

An extension of an improved forced based design procedure for 3D steel structures

This paper proposes an extension of the Improved Forced Based Design procedure to 3D steel structures. The Improved Forced Based Design (IFBD) procedure consists of a more rational sequence of the design checks proposed in Eurocode 8 and involves a more realistic selection of the behaviour factor instead of selecting an empirical value based on the ductility class and lateral resisting system adopted. The design procedure was tested on a group of four 3D steel structures, composed by moment-resisting frames with three storeys height and the same plan configuration in all storeys. The plan configuration was defined in order to target lateral restrained or unrestrained systems as well as plan regular or irregular structures. The same group of structures was also designed according to the force-based process prescribed in Eurocode 8. The member sizes obtained through the two approaches were compared and the seismic performance was assessed through nonlinear static and time-history analyses. The limit states referred to structural and non-structural damage, considering the two levels design approach, which are the serviceability and the ultimate limit states, were examined. The results obtained reveal that the IFBD leads to more economical structures that still comply with the performance requirements prescribed in Eurocode 8.

It is not about what you read, but how you read it: the effects of sequencing rational and emotional messages on corporate and product brand attitudes

The study aims to examine the effects of message sequencing on attitudinal responses, namely on the effects of rational to emotional and emotional to rational message sequences on consumer attitudes toward the corporate and product brand. A between-subjects experiment was conducted using a stratified random sample. Participants were exposed to advertisements that communicate marketing messages either in a rational to emotional sequence or in an emotional to rational sequence for a product advertised by an organization. All attitudinal responses to the corporate and product brand were collected through a questionnaire that was given at the end of the experiment. The findings suggest advertisements that communicate marketing messages in an emotional to rational sequence produced more favorable attitudes toward the product brand among consumers as compared to advertisements that communicate in a rational to emotional sequence. However, no significant differences were found on consumer attitudes toward the corporate brand. These results remain true regardless of gender.

Serology in the Digital Age: Using Long Synthetic Peptides Created from Nucleic Acid Sequences as Antigens in Microarrays.

Background: Antibodies to microbes, or to autoantigens, are important markers of disease. Antibody detection (serology) can reveal both past and recent infections. There is a great need for development of rational ways of detecting and quantifying antibodies, both for humans and animals. Traditionally, serology using synthetic antigens covers linear epitopes using up to 30 amino acid peptides. Methods: We here report that peptides of 100 amino acids or longer (“megapeptides”), designed and synthesized for optimal serological performance, can successfully be used as detection antigens in a suspension multiplex immunoassay (SMIA). Megapeptides can quickly be created just from pathogen sequences. A combination of rational sequencing and bioinformatic routines for definition of diagnostically-relevant antigens can, thus, rapidly yield efficient serological diagnostic tools for an emerging infectious pathogen. Results: We designed megapeptides using bioinformatics and viral genome sequences. These long peptides were tested as antigens for the presence of antibodies in human serum to the filo-, herpes-, and polyoma virus families in a multiplex microarray system. All of these virus families contain recently discovered or emerging infectious viruses. Conclusion: Long synthetic peptides can be useful as serological diagnostic antigens, serving as biomarkers, in suspension microarrays.

A comprehensive review of genomic landscape, biomarkers and treatment sequencing in castration-resistant prostate cancer.

Hormone-naïve prostate cancer and its castration-resistant state (CRPC) are clinically and genetically heterogeneous diseases. From initiation of prostate carcinogenesis to its evolution towards therapeutic resistance, various combinations of genetic and epigenetic events occur. Schematically, progression to CRPC could be divided in two distinct pathways, either dependent or independent of the androgen receptor activity. Nevertheless, because the better knowledge of the genetic landscape of CRPC is under way, limited clinical applications are available at the moment, underlying the usefulness of prognostic and predictive biomarkers in daily practice. Despite the promising prognostic value of circulating tumor cells, no biomarker has been currently validated as a surrogate for overall survival in CRPC patients. Inversely, considerable interest has been generated with the recent finding of the splice variant AR-V7 that allows to predict resistance to abiraterone acetate and enzalutamide. However, other predictive biomarkers would be necessary to accurately guide personalized sequencing of CRPC treatment, which now includes numerous possibilities based on the six validated drugs, without accounting for those currently under investigation in the ongoing randomized controlled trials. As a consequence, only rational sequencing, which consists in choosing an agent that is not expected to have cross-resistance with previous therapy, can be currently advised.

Derecho penal económico. Perspectiva integrada.

Abstract. Regarding the concept of economic criminal law, within the context of traditional positions – the restrictive one, the open one and the eclectic critical approaches, the author aims at a conceptual approach integrated into this sophisticated area of criminal law. This integrated attitude does not arise as the mere result of the synthesis of the abovementioned traditional positions, but it is made up of other significant variables, to wit: the ontic conditions of social structure, a social anthropocentric vision, the vindication of a need for a very precise legally protected right and determining in its offensiveness, and the claim for the democratic legitimacy of this regulatory category.All of this is combined with a concrete reality which summarizes an illicit behavior or objectively abusive with respect to the normal functioning of the economy’s mechanisms or resources. Based on these ideas, a rational and minimalist sequence between the concepts of economic criminal law, macroeconomic criminal law and business criminal law may be attained, in addition to a common methodology to typify and bring these phenomena closer together and a more fluent exercise of the guarantees of a social democratic rule of law.

Heated tool ultrasonic welding of elastic containers produced from fluoroplastic film

It is shown that the application of heated tool ultrasonic welding of elastic containers using the welding process proposed in the article and the rational sequence of assembling and welding operations improve the productivity of the welding process and also the quality and strength of the welded joint, whilst reducing the material and labour costs.

Revisiting the construction of the real line

The most common constructions of the reals from the rationals attempt to “fill in the gaps,” using (equivalence classes of) Cauchy sequences of rationals or Dedekind cuts. Another technique, more familiar to logicians than to analysts, is to expand the set Q of rationals to the set QN of all sequences of rationals and then collapse back down in two stages. In the first stage, produce a structure X by identifying sequences that agree on a large set; in the second stage, further collapse X by removing its infinite elements, and collapsing its infinitesimals to 0. The latter approach, though less widely known, provides, in its intermediary construction X, an example of interesting pathologies. We show here that X is an example of a complete (even open-complete) ordered field that is not Archimedean. The field X showcases the significant role of the Archimedean property in the structure of R, as many of the fundamental properties of R are found to fail in X precisely because this property is absent, such as the Heine-Borel Theorem and the Bolzano-Weierstrass Theorem. Indeed, we show that the field X is not even metrizable. We conclude with a detailed proof, accessible to non-logicians, that the second stage of the construction succeeds in producing a complete Archimedean ordered field. Mathematics Subject Classification: 12J15, 26E35

Case Sequencing of Diagnostic Imaging Studies Performed Under General Anesthesia or Monitored Anesthesia Care During Nights and Weekends.

General anesthesia or monitored anesthesia care sometimes is provided in nonoperating room (OR) locations during nights and weekends (e.g., for magnetic resonance imaging [MRI] or computerized tomography [CT]). Rational and consistent scheduling and sequencing decisions for these diagnostic imaging procedures, including coordination with OR cases, cannot be done without knowing how long each case can wait to be started without risking a worsening of the patient's condition. We reviewed the medical records of the 81 patients who underwent diagnostic imaging procedures (78 = MRI, 3 = CT scan) under general anesthesia or monitored anesthesia care either on weekends or between 6 pm and 6 am at the University of Iowa Hospitals between March 2012 and February 2014. For 77.8% of patients, the indications could have changed clinical management within 4 hours (N = 63/81). Among the 63 imaging studies with potential immediate impact, there was documentation of results having been communicated to the treating team within 4 hours of the completion of imaging for 39 of the patients. Among the 39 patients, 15 promptly received medications or underwent procedures based on the imaging results. Thus, 15 of the 81 patients had a change in care (18.5%, 95% lower confidence limit = 11.2%). Our results are important since we showed previously that it is not possible to make rational and consistent decisions in case sequencing without knowing how long each case (including diagnostic imaging procedures) can wait to be started without a change in the patient's risk. The scheduled surgical procedure itself provides sufficient information to assess safe waiting times to start add-on cases (e.g., appendectomy). In contrast, MRI provides no context as to how potential findings will influence treatment. Our results show that the assumption cannot reasonably be made when sequencing cases that all imaging studies can or cannot wait longer than pending surgical procedures. Our results show that, for evidence-based OR management decision-making, information to decide appropriate waiting should be obtained electronically or verbally for each imaging study.

Task formalization of constructing rational product assembly sequence

Запропоновано математичні моделі, що відображають зв’язки базування та розмірні зв’язки між складальними елементами, враховують обмеження доступу, конструкторсько-технологічні та екологічні критерії складальних процесів. З їх використанням розроблено метод синтезу раціональної послідовності складання виробів, який відрізняється тим, що при побудові варіантів послідовності складання враховується комплекс технологічних та екологічних обмежень, що дозволяє забезпечити задану якість виробу та мінімізувати тривалість виробничого циклу.

Automatic intellectual signal processing in subsurface radar systems

A technique for automatic digital processing of the ice-coating radargrams, which is based on a rational sequence of modern intellectual data analysis methods, is discussed. It is demonstrated that the structural analysis of georadar measurements is an efficient tool in extracting an informative signal and investigating its cluster structure, followed by estimating the river ice thickness with the help of locally weighted polynomial-regression models and the expectation-maximization algorithm.

Teaching of real numbers by using the Archimedes–Cantor approach and computer algebra systems

Computer technologies and especially computer algebra systems (CAS) allow students to overcome some of the difficulties they encounter in the study of real numbers. The teaching of calculus can be considerably more effective with the use of CAS provided the didactics of the discipline makes it possible to reveal the full computational potential of CAS. In the case of real numbers, the Archimedes–Cantor approach satisfies this requirement. The name of Archimedes brings back the exhaustion method. Cantor's name reminds us of the use of Cauchy rational sequences to represent real numbers. The usage of CAS with the Archimedes–Cantor approach enables the discussion of various representations of real numbers such as graphical, decimal, approximate decimal with precision estimates, and representation as points on a straight line. Exercises with numbers such as e, π, the golden ratio ϕ, and algebraic irrational numbers can help students better understand the real numbers. The Archimedes–Cantor approach also reveals a deep and close relationship between real numbers and continuity, in particular the continuity of functions.

Boosting antibody developability through rational sequence optimization

The application of monoclonal antibodies as commercial therapeutics poses substantial demands on stability and properties of an antibody. Therapeutic molecules that exhibit favorable properties increase the success rate in development. However, it is not yet fully understood how the protein sequences of an antibody translates into favorable in vitro molecule properties. In this work, computational design strategies based on heuristic sequence analysis were used to systematically modify an antibody that exhibited a tendency to precipitation in vitro. The resulting series of closely related antibodies showed improved stability as assessed by biophysical methods and long-term stability experiments. As a notable observation, expression levels also improved in comparison with the wild-type candidate. The methods employed to optimize the protein sequences, as well as the biophysical data used to determine the effect on stability under conditions commonly used in the formulation of therapeutic proteins, are described. Together, the experimental and computational data led to consistent conclusions regarding the effect of the introduced mutations. Our approach exemplifies how computational methods can be used to guide antibody optimization for increased stability.

RESEARCH OF ENGINEERING TRAFFIC IN COMPUTER UZ NETWORK USING MPLS TE TECHNOLOGY

Purpose. In railway transport of Ukraine one requires the use of computer networks of different technologies: Ethernet, Token Bus, Token Ring, FDDI and others. In combined computer networks on the railway transport it is necessary to use packet switching technology in multiprotocol networks MPLS (MultiProtocol Label Switching) more effectively. They are based on the use of tags. Packet network must transmit different types of traffic with a given quality of service. The purpose of the research is development a methodology for determining the sequence of destination flows for the considered fragment of computer network of UZ. Methodology. When optimizing traffic management in MPLS networks has the important role of technology traffic engineering (Traffic Engineering, TE). The main mechanism of TE in MPLS is the use of unidirectional tunnels (MPLS TE tunnel) to specify the path of the specified traffic. The mathematical model of the problem of traffic engineering in computer network of UZ technology MPLS TE was made. Computer UZ network is represented with the directed graph, their vertices are routers of computer network, and each arc simulates communication between nodes. As an optimization criterion serves the minimum value of the maximum utilization of the TE-tunnel. Findings. The six options destination flows were determined; rational sequence of flows was found, at which the maximum utilization of TE-tunnels considered a simplified fragment of a computer UZ network does not exceed 0.5. Originality. The method of solving the problem of traffic engineering in Multiprotocol network UZ technology MPLS TE was proposed; for different classes its own way is laid, depending on the bandwidth and channel loading. Practical value. Ability to determine the values of the maximum coefficient of use of TE-tunnels in computer UZ networks based on developed software model «TraffEng». The input parameters of the model: number of routers, channel capacity, the number of streams, the average flow velocity. As a result of the model «TraffEng» choices variants of TE-tunnels with the corresponding values of the maximum coefficient of their use are presented.

Abstract 3232: Optimization of a potent stapled helix peptide that binds to Replication Protein A

Abstract Replication Protein A (RPA) is a major regulator of checkpoint activation and enhanced DNA repair in cancer cells. In response to genotoxic stress, the RPA complex binds to and protects ssDNA while serving as a scaffold to recruit critical checkpoint and DNA-damage response proteins through the N-terminal region of the 70 kDa subunit of RPA (RPA70N). Specific disruption of the protein-protein interactions mediated by the RPA70N domain has the potential to produce selective killing of cancer cells without the risk of cytotoxicity due to interference in the ssDNA-binding function. Stapled helix peptides can serve as useful tools for inhibiting protein-protein interactions. However, their utility can be limited due to difficulties often encountered during attempts to improve the binding affinity to the target. Here, we report the discovery and optimization of a potent stapled helix peptide probe, derived from the endogenous RPA binding partner ATRIP (ATR-interacting protein), that binds to and inhibits the RPA70N protein-protein interaction surface. Alanine scanning, charge abrogation, and rational sequence optimization resulted in a peptide with a 100-fold potency gain over the native sequence and improved physical characteristics. In addition to the application of these traditional strategies, we describe a novel approach for efficiently designing peptides containing unnatural amino acids. This method involves the incorporation of an unnatural amino acid inspired by the structure activity relationships of small molecules that bind to the same site on the protein. Use of this approach produced stapled peptides with dramatic increases in binding affinity to RPA70N relative to aooIn al peptide containing only natural amino acids. The optimized peptides are cell penetrant, able to enter the nucleus, and co-localize with RPA in the nucleus at sites of DNA damage. Such a peptide may serve as a probe molecule to explore both the effects of RPA inhibition on the DNA damage response and the therapeutic potential of RPA inhibition as a cancer target. Citation Format: Alex G. Waterson, Andreas O. Frank, Bhavatarini Vandgamudi, Michael D. Feldkamp, Elaine M. Souza-Fagundes, Jessica W. Luzwick, David Cortez, Edward T. Olejniczak, Olivia W. Rossanese, Walter J. Chazin, Stephen W. Fesik. Optimization of a potent stapled helix peptide that binds to Replication Protein A. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3232. doi:10.1158/1538-7445.AM2014-3232

Nursing Experience of 850 Cases on Centralized Cataract Surgery in CDPF Eyesight Recovery Project

<strong>Objective</strong>: To explore and summarize the nursing experience of concentrated cataract surgery in CDPF eyesight recovery project. <strong>Method</strong>: Retrospective analysis of the nursing experience in 850 cases of CDPF cataract patients admitted to the hospital for surgery from June 2008 to July 2014. <strong>Results</strong>:<strong> </strong>Through planned, organized education of related knowledge, all patients showed stable emotion and good attitude. They could greatly cooperate in the surgery and master postoperative self-care methods. With fewer complications, their visual acuity was improved to a certain degree. They all reached the standards of blindness exclusion. <strong>Conclusion</strong>: Organized arrangements, centralized health education, perfect preoperative examination, rational sequencing, careful check and meticulous care were the successful basis of centralized cataract surgery in CDPF eyesight recovery project, which guaranteed a smooth surgery without errors or accidents.

Radium-223 in metastatic castration resistant prostate cancer

In 2004, docetaxel was approved for the treatment of metastatic castration-resistant prostate cancer (mCRPC). For the next several years, there was a lull in drug approvals. However, from 2010 onwards, 5 additional therapies have been approved on the basis of showing a survival benefit in phase III studies. These agents include sipuleucel-T, cabazitaxel, abiraterone, enzalutamide and (most recently) radium-223. Amongst radiopharmaceuticals currently used for advanced prostate cancer (e.g. samarium-153 and strontium-89), radium-223 possesses several unique properties. As an alpha-emitting compound, the agent produces a high-energy output over a short range, facilitating selective destruction of tissue within the bone in the region of osteoblastic lesions while sparing surrounding normal tissue. The current review will outline biological rationale for radium-223 and also provide an overview of preclinical and clinical development of the agent. Rational sequencing of radium-223 and combinations, in the increasingly complex landscape of mCRPC will be discussed, along with factors influencing clinical implementation.

Rationally designed treatment for metastatic colorectal cancer: current drug development strategies.

The therapeutic landscape of metastatic colorectal cancer (mCRC) has changed substantially with the emergence of new molecularly targeted agents (MTA) used as single agents or alongside standard chemotherapy. The use of these MTAs extended the overall survival of patients with mCRC to a level that current chemotherapeutics alone could not achieve. In addition, improvement in surgical techniques and ablation modalities offer cure to a limited subset of patients with mCRC and MTAs have been found to have a significant role here too, as they aid resectability. However, for the majority of patients, mCRC remains an invariably incurable disease necessitating continued courses of combined treatment modalities. During the course of these treatments, either cytotoxic or biological, cancer cells maintain their ability to acquire mitogenic mutations which render them resistant to treatment. Key challenges remain to identify appropriate subsets of patients who will most likely benefit from these new MTAs and effectively select these based on validated biomarkers. Moreover, better knowledge of the biology of colorectal cancer and the mechanisms via which it bypasses blockade of known signalling pathways will help us design better and more rational sequencing of these treatments, so that we can maximise the survivorship of mCRC patients. This review outlines treatment strategies for known molecular alterations with new MTAs and highlights some promising strategies.

Catalog Learning: Carabid Beetles Learn to Manipulate with Innate Coherent Behavioral Patterns

One of the most fascinating problems in comparative psychology is how learning contributes to solving specific functional problems in animal life, and which forms of learning our species shares with non-human animals. Simulating a natural situation of territorial conflicts between predatory carabids and red wood ants in field and laboratory experiments, we have revealed a relatively simple and quite natural form of learning that has been overlooked. We call it catalog learning, the name we give to the ability of animals to establish associations between stimuli and coherent behavioral patterns (patterns consist of elementary motor acts that have a fixed order). Instead of budgeting their motor acts gradually, from chaotic to rational sequences in order to learn something new, which is characteristic for a conditioning response, animals seem to be “cataloguing” their repertoire of innate coherent behavioral patterns in order to optimize their response to a certain repetitive event. This form of learning can be described as “stimulus-pattern” learning. In our experiments four “wild” carabid species, whose cognitive abilities have never been studied before, modified their behavior in a rather natural manner in order to avoid damage from aggressive ants. Beetles learned to select the relevant coherent behavioral patterns from the set of seven patterns, which are common to all four species and apparently innate. We suggest that this form of learning differs from the known forms of associative learning, and speculate that it is quite universal and can be present in a wide variety of species, both invertebrate and vertebrate. This study suggests a new link between the concepts of cognition and innateness.

МодернизированныЙ метоД составлениЯ грАфикА движениЯ поездоВ С учетоМ потерЬ электроэнергИИ

Вдосконалений метод складання графіка руху поїздів, який відрізняється від існуючого застосуванням евристичного алгоритму для визначення раціональної послідовності відправлення і інтервалів між поїздами. Використання даного методу дає можливість мінімізувати втрати електроенергії в тягової мережі

Logistiks methods of resource management at repairs of the rolling-stock at the industrial enterprises

In clause the methodical approaches to logistics methods of resource management of the industrial enterprises are considered at performance of repairs of the rolling-stock. The general(common) algorithm of increase of capacity of system of repair is offered on the basis of a choice of a rational sequence of realization of methods of management of its(her) resources.